Abstract
A sophisticated laser microbeam system has been combined with an image-processing computer system (Gould-De Anza IP5500) to study numerous problems in ceil biology and genetics. The lasers are two Nd:YAG lasers with output in the picosecond (25–100 psec) and nanosecond (10-nsec) domains and wavelengths at 1.06 μm, 532 nm, 355 nm, and 266 nm. In addition, a dye laser is pumped with the nanosecond 532-nm and 355-nm YAG wavelengths to yield a tunable spectrum of 217–800 nm. Cellular systems are observed through a Zeiss Axiomat microscope that focuses the laser beam to spot diameters of less than 1 μm. The microscope images are directed to either a Newvicon or low-light, intensified television camera and subsequently directed to the computer system for real-time digitization, manipulation, and redisplay. Cellular systems studied by this approach are cell migration, function of the mitotic apparatus, myocardial cell contractility, and gene expression. (Invited paper, 30 min.)
© 1984 Optical Society of America
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