Fast 3D-resolved fluorescence and phosphorescence lifetime imaging in a sample consisting of human fibroblasts stained with Rhodamine DHPE, seeded inside a collagen matrix and treated, in a PBS buffer containing a 1 mM TDRT ruthenium-based oxygen sensor. PLIM images were acquired at 300 kHz modulation frequency. PLIM movie sequences corresponding to phosphorescence lifetime-resolved image from phase data measurements throughout a 3D matrix. For details, see Choi et al., Opt. Express 20, 26219 (2012).