Abstract

We demonstrate subwavelength sectioning on biological samples with a conventional confocal microscope. This optical sectioning is achieved by the phenomenon of supercritical angle fluorescence, wherein only a fluorophore next to the interface of a refractive index discontinuity can emit propagating components of radiation into the so-called forbidden angles. The simplicity of this technique allows it to be integrated with a high numerical aperture confocal scanning microscope by only a simple modification on the detection channel. Confocal-supercritical angular fluorescence microscopy would be a powerful tool to achieve high-resolution surface imaging, especially for membrane imaging in biological samples.

© 2014 Optical Society of America

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2013 (1)

D. Axelrod, Biophys. J. 104, 1401 (2013).
[CrossRef]

2012 (1)

T. Barroca, K. Balaa, S. Lévêque-Fort, and E. Fort, Phys. Rev. Lett. 108, 218101 (2012).
[CrossRef]

2011 (2)

2010 (1)

C. M. Winterflood, T. Ruckstuhl, D. Verdes, and S. Seeger, Phys. Rev. Lett. 105, 108103 (2010).
[CrossRef]

2009 (1)

2008 (1)

E. Fort and S. Grésillon, J. Phys. D 41, 013001 (2008).
[CrossRef]

2007 (1)

2006 (2)

J. P. Overington, B. Al-Lazikani, and A. L. Hopkins, Nat. Rev. Drug Discov. 5, 993 (2006).
[CrossRef]

A. L. Mattheyses and D. Axelrod, J. Biomed. Opt. 11, 014006 (2006).
[CrossRef]

2004 (2)

2003 (1)

T. Ruckstuhl, M. Rankl, and S. Seeger, Biosens. Bioelectron. 18, 1193 (2003).
[CrossRef]

2001 (1)

D. Axelrod, J. Biomed. Opt. 6, 6 (2001).
[CrossRef]

2000 (1)

T. Ruckstuhl, J. Enderlein, S. Jung, and S. Seeger, Anal. Chem. 72, 2117 (2000).
[CrossRef]

Al-Lazikani, B.

J. P. Overington, B. Al-Lazikani, and A. L. Hopkins, Nat. Rev. Drug Discov. 5, 993 (2006).
[CrossRef]

Axelrod, D.

D. Axelrod, Biophys. J. 104, 1401 (2013).
[CrossRef]

A. L. Mattheyses and D. Axelrod, J. Biomed. Opt. 11, 014006 (2006).
[CrossRef]

D. Axelrod, J. Biomed. Opt. 6, 6 (2001).
[CrossRef]

Balaa, K.

T. Barroca, K. Balaa, S. Lévêque-Fort, and E. Fort, Phys. Rev. Lett. 108, 218101 (2012).
[CrossRef]

T. Barroca, K. Balaa, J. Delahaye, S. Lévêque-Fort, and E. Fort, Opt. Lett. 36, 3051 (2011).
[CrossRef]

Barroca, T.

T. Barroca, K. Balaa, S. Lévêque-Fort, and E. Fort, Phys. Rev. Lett. 108, 218101 (2012).
[CrossRef]

T. Barroca, K. Balaa, J. Delahaye, S. Lévêque-Fort, and E. Fort, Opt. Lett. 36, 3051 (2011).
[CrossRef]

Chon, J. W.

Chung, E.

Delahaye, J.

Enderlein, J.

T. Ruckstuhl, J. Enderlein, S. Jung, and S. Seeger, Anal. Chem. 72, 2117 (2000).
[CrossRef]

Fort, E.

T. Barroca, K. Balaa, S. Lévêque-Fort, and E. Fort, Phys. Rev. Lett. 108, 218101 (2012).
[CrossRef]

T. Barroca, K. Balaa, J. Delahaye, S. Lévêque-Fort, and E. Fort, Opt. Lett. 36, 3051 (2011).
[CrossRef]

E. Fort and S. Grésillon, J. Phys. D 41, 013001 (2008).
[CrossRef]

Grésillon, S.

E. Fort and S. Grésillon, J. Phys. D 41, 013001 (2008).
[CrossRef]

Gu, M.

Hopkins, A. L.

J. P. Overington, B. Al-Lazikani, and A. L. Hopkins, Nat. Rev. Drug Discov. 5, 993 (2006).
[CrossRef]

Jung, S.

T. Ruckstuhl, J. Enderlein, S. Jung, and S. Seeger, Anal. Chem. 72, 2117 (2000).
[CrossRef]

Kano, H.

Kim, Y.-H.

Lévêque-Fort, S.

T. Barroca, K. Balaa, S. Lévêque-Fort, and E. Fort, Phys. Rev. Lett. 108, 218101 (2012).
[CrossRef]

T. Barroca, K. Balaa, J. Delahaye, S. Lévêque-Fort, and E. Fort, Opt. Lett. 36, 3051 (2011).
[CrossRef]

Mattheyses, A. L.

A. L. Mattheyses and D. Axelrod, J. Biomed. Opt. 11, 014006 (2006).
[CrossRef]

Overington, J. P.

J. P. Overington, B. Al-Lazikani, and A. L. Hopkins, Nat. Rev. Drug Discov. 5, 993 (2006).
[CrossRef]

Rankl, M.

T. Ruckstuhl, M. Rankl, and S. Seeger, Biosens. Bioelectron. 18, 1193 (2003).
[CrossRef]

Ruckstuhl, T.

T. Ruckstuhl, D. Verdes, C. Winterflood, and S. Seeger, Opt. Express 19, 6836 (2011).
[CrossRef]

C. M. Winterflood, T. Ruckstuhl, D. Verdes, and S. Seeger, Phys. Rev. Lett. 105, 108103 (2010).
[CrossRef]

T. Ruckstuhl and D. Verdes, Opt. Express 12, 4246 (2004).
[CrossRef]

T. Ruckstuhl, M. Rankl, and S. Seeger, Biosens. Bioelectron. 18, 1193 (2003).
[CrossRef]

T. Ruckstuhl, J. Enderlein, S. Jung, and S. Seeger, Anal. Chem. 72, 2117 (2000).
[CrossRef]

Seeger, S.

T. Ruckstuhl, D. Verdes, C. Winterflood, and S. Seeger, Opt. Express 19, 6836 (2011).
[CrossRef]

C. M. Winterflood, T. Ruckstuhl, D. Verdes, and S. Seeger, Phys. Rev. Lett. 105, 108103 (2010).
[CrossRef]

T. Ruckstuhl, M. Rankl, and S. Seeger, Biosens. Bioelectron. 18, 1193 (2003).
[CrossRef]

T. Ruckstuhl, J. Enderlein, S. Jung, and S. Seeger, Anal. Chem. 72, 2117 (2000).
[CrossRef]

Sheppard, C. J.

So, P. T.

Tang, W. T.

Terakado, G.

Verdes, D.

Watanabe, K.

Winterflood, C.

Winterflood, C. M.

C. M. Winterflood, T. Ruckstuhl, D. Verdes, and S. Seeger, Phys. Rev. Lett. 105, 108103 (2010).
[CrossRef]

Anal. Chem. (1)

T. Ruckstuhl, J. Enderlein, S. Jung, and S. Seeger, Anal. Chem. 72, 2117 (2000).
[CrossRef]

Appl. Opt. (2)

Biophys. J. (1)

D. Axelrod, Biophys. J. 104, 1401 (2013).
[CrossRef]

Biosens. Bioelectron. (1)

T. Ruckstuhl, M. Rankl, and S. Seeger, Biosens. Bioelectron. 18, 1193 (2003).
[CrossRef]

J. Biomed. Opt. (2)

D. Axelrod, J. Biomed. Opt. 6, 6 (2001).
[CrossRef]

A. L. Mattheyses and D. Axelrod, J. Biomed. Opt. 11, 014006 (2006).
[CrossRef]

J. Phys. D (1)

E. Fort and S. Grésillon, J. Phys. D 41, 013001 (2008).
[CrossRef]

Nat. Rev. Drug Discov. (1)

J. P. Overington, B. Al-Lazikani, and A. L. Hopkins, Nat. Rev. Drug Discov. 5, 993 (2006).
[CrossRef]

Opt. Express (3)

Opt. Lett. (1)

Phys. Rev. Lett. (2)

T. Barroca, K. Balaa, S. Lévêque-Fort, and E. Fort, Phys. Rev. Lett. 108, 218101 (2012).
[CrossRef]

C. M. Winterflood, T. Ruckstuhl, D. Verdes, and S. Seeger, Phys. Rev. Lett. 105, 108103 (2010).
[CrossRef]

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