Abstract

Quantitative fluorescence spectroscopic Mueller matrix measurements from the connective tissue regions of human cervical tissue reveal intriguing fluorescence diattenuation and polarizance effects. Interestingly, the estimated fluorescence linear diattenuation and polarizance parameters were considerably reduced in the precancerous tissues as compared to the normal ones. These polarimetry effects of the autofluorescence were found to originate from anisotropically organized collagen molecular structures present in the connective tissues. Consequently, the reduction of the magnitude of these polarimetric parameters at higher grades of precancer was attributed to the loss of anisotropic organization of collagen, which was also confirmed by control experiments. These results indicate that fluorescence spectral diattenuation and polarizance parameters may serve as potentially useful diagnostic metrics.

© 2014 Optical Society of America

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N. Ghosh and I. A. Vitkin, J. Biomed. Opt. 16, 110801 (2011).
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V. V. Tuchin, L. Wang, and D. À. Zimnyakov, Optical Polarization in Biomedical Applications (Springer-Verlag, 2006).

Appl. Opt. (1)

Biophys. J. (1)

D. Arifler, I. Pavlova, A. Gillenwater, and R. R. Kortum, Biophys. J. 92, 3260 (2007).
[CrossRef]

Connect. Tissue Res. (1)

P. F. Davison, D. J. Cannon, and L. P. Andersson, Connect. Tissue Res. 1, 205 (1972).
[CrossRef]

IEEE J. Quantum Electron. (1)

R. R. Alfano, G. C. Tang, A. Pradhan, W. Lam, D. S. J. Choy, and E. Opher, IEEE J. Quantum Electron. 23, 1806 (1987).
[CrossRef]

J. Am. Chem. Soc. (1)

U. Eliav and G. Navon, J. Am. Chem. Soc. 128, 15956 (2006).
[CrossRef]

J. Biomed. Opt. (3)

R. Drezek, K. Sokolov, U. Utzinger, I. Boiko, A. Malpica, M. Follen, and R. R. Kortum, J. Biomed. Opt. 6, 385 (2001).
[CrossRef]

Y. Wu and J. Y. Qu, J. Biomed. Opt. 11, 054023 (2006).
[CrossRef]

N. Ghosh and I. A. Vitkin, J. Biomed. Opt. 16, 110801 (2011).
[CrossRef]

J. Opt. Soc. Am. A (1)

Nat. Rev. Cancer (1)

N. Thekkek and R. R. Kortum, Nat. Rev. Cancer 8, 725 (2008).
[CrossRef]

Neoplasia (1)

N. Ramanujam, Neoplasia 2, 89 (2000).
[CrossRef]

Oncogene (1)

T. Bogenrieder and M. Herlyn, Oncogene 22, 6524 (2003).
[CrossRef]

Opt. Express (3)

Opt. Lett. (2)

Other (2)

J. Lackowicz, Principles of Fluorescence Spectroscopy (Plenum, 1983).

V. V. Tuchin, L. Wang, and D. À. Zimnyakov, Optical Polarization in Biomedical Applications (Springer-Verlag, 2006).

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Figures (3)

Fig. 1.
Fig. 1.

4×4 fluorescence spectroscopic (λem=475750nm) Mueller matrix recorded (in the exact backscattering configuration) with 405 nm excitation, from the connective tissue region (stroma) of a typical grade-III precancerous tissue section.

Fig. 2.
Fig. 2.

Spectral variation of (a) fluorescence linear diattenuation dlinfl and (b) fluorescence linear polarizance plinfl parameters (shown by triangles) for normal (open symbol) and precancerous (solid symbol) connective tissue sections. The insets of (a) and (b) show the fluorescence circular diattenuation and circular polarizance parameters, respectively (shown by circles). The values of the parameters reported here are the mean values over 15 precancerous and five normal tissue sections, respectively, and the error bars represent corresponding standard deviations.

Fig. 3.
Fig. 3.

Spectral variations of (a) fluorescence linear diattenuation dlinfl and (b) fluorescence linear polarizance plinfl for the untreated (open triangle) and acetic-acid-treated (solid triangle) type-I bovine collagen samples.

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