Abstract

We present a method, intensity fluctuation modulation (IFM), to obtain a full-field laser speckle microvessel image. Different from laser speckle contrast analysis, IFM imaging is insensitive to flow velocity and can be used to reconstruct microvessel images with higher spatial resolution and SNR. An in vivo animal experiment on a mouse pinna is conducted to demonstrate that IFM imaging is capable of achieving laser speckle microangiography.

© 2013 Optical Society of America

Full Article  |  PDF Article

References

  • View by:
  • |
  • |
  • |

  1. G. Michelson, J. Welzenbach, I. Pal, and J. Harazny, Br. J. Ophthalmol. 82, 1294 (1998).
    [CrossRef]
  2. A. Serov, B. Steinacher, and T. Lasser, Opt. Express 13, 3681 (2005).
    [CrossRef]
  3. M. Atlan, M. Gross, B. C. Forget, T. Vitalis, A. Rancillac, and A. K. Dunn, Opt. Lett. 31, 2762 (2006).
    [CrossRef]
  4. M. Atlan and M. Gross, Rev. Sci. Instrum. 77, 116103 (2006).
    [CrossRef]
  5. A. Serov and T. Lasser, Opt. Express 13, 6416 (2005).
    [CrossRef]
  6. M. Draijer, E. Hondebrink, T. Leeuwen, and W. Steenbergen, Opt. Express 17, 3211 (2009).
    [CrossRef]
  7. J. D. Briers and S. Webster, Opt. Commun. 116, 36 (1995).
    [CrossRef]
  8. A. K. Dunn, H. Bolay, M. A. Moskowitz, and D. A. Boas, J. Cereb. Blood Flow Metab. 21, 195 (2001).
    [CrossRef]
  9. H. Cheng, Q. Luo, Z. Wang, H. Gong, S. Chen, and W. Liang, Appl. Opt. 42, 5759 (2003).
    [CrossRef]
  10. B. Choi, N. M. Kang, and S. Nelson, Microvasc. Res. 68, 143 (2004).
    [CrossRef]
  11. H. Cheng, Q. Luo, Q. Liu, Q. Lu, H. Gong, and S. Zeng, Phys. Med. Biol. 49, 1347 (2004).
    [CrossRef]
  12. P. Li, S. Ni, L. Zhang, S. Zeng, and Q. Luo, Opt. Lett. 31, 1824 (2006).
    [CrossRef]
  13. H. Cheng, Y. Yan, and T. Q. Duong, Opt. Express 16, 10214 (2008).
    [CrossRef]
  14. D. Wang, A. Moyer, and M. Henderson, Appl. Opt. 51, 7576 (2012).
    [CrossRef]
  15. M. Paturzo, A. Finizio, P. Memmolo, R. Puglisi, D. Balduzzi, A. Galli, and P. Ferraro, Lab Chip 12, 3073 (2012).
    [CrossRef]
  16. V. Bianco, M. Paturzo, A. Finizio, D. Balduzzi, R. Puglisi, A. Galli, and P. Ferraro, Opt. Lett. 37, 4212 (2012).
    [CrossRef]
  17. S. Yuan, A. Devor, D. A. Boas, and A. K. Dunn, Appl. Opt. 44, 1823 (2005).
    [CrossRef]

2012

2009

2008

2006

2005

2004

B. Choi, N. M. Kang, and S. Nelson, Microvasc. Res. 68, 143 (2004).
[CrossRef]

H. Cheng, Q. Luo, Q. Liu, Q. Lu, H. Gong, and S. Zeng, Phys. Med. Biol. 49, 1347 (2004).
[CrossRef]

2003

2001

A. K. Dunn, H. Bolay, M. A. Moskowitz, and D. A. Boas, J. Cereb. Blood Flow Metab. 21, 195 (2001).
[CrossRef]

1998

G. Michelson, J. Welzenbach, I. Pal, and J. Harazny, Br. J. Ophthalmol. 82, 1294 (1998).
[CrossRef]

1995

J. D. Briers and S. Webster, Opt. Commun. 116, 36 (1995).
[CrossRef]

Atlan, M.

Balduzzi, D.

M. Paturzo, A. Finizio, P. Memmolo, R. Puglisi, D. Balduzzi, A. Galli, and P. Ferraro, Lab Chip 12, 3073 (2012).
[CrossRef]

V. Bianco, M. Paturzo, A. Finizio, D. Balduzzi, R. Puglisi, A. Galli, and P. Ferraro, Opt. Lett. 37, 4212 (2012).
[CrossRef]

Bianco, V.

Boas, D. A.

S. Yuan, A. Devor, D. A. Boas, and A. K. Dunn, Appl. Opt. 44, 1823 (2005).
[CrossRef]

A. K. Dunn, H. Bolay, M. A. Moskowitz, and D. A. Boas, J. Cereb. Blood Flow Metab. 21, 195 (2001).
[CrossRef]

Bolay, H.

A. K. Dunn, H. Bolay, M. A. Moskowitz, and D. A. Boas, J. Cereb. Blood Flow Metab. 21, 195 (2001).
[CrossRef]

Briers, J. D.

J. D. Briers and S. Webster, Opt. Commun. 116, 36 (1995).
[CrossRef]

Chen, S.

Cheng, H.

Choi, B.

B. Choi, N. M. Kang, and S. Nelson, Microvasc. Res. 68, 143 (2004).
[CrossRef]

Devor, A.

Draijer, M.

Dunn, A. K.

Duong, T. Q.

Ferraro, P.

M. Paturzo, A. Finizio, P. Memmolo, R. Puglisi, D. Balduzzi, A. Galli, and P. Ferraro, Lab Chip 12, 3073 (2012).
[CrossRef]

V. Bianco, M. Paturzo, A. Finizio, D. Balduzzi, R. Puglisi, A. Galli, and P. Ferraro, Opt. Lett. 37, 4212 (2012).
[CrossRef]

Finizio, A.

V. Bianco, M. Paturzo, A. Finizio, D. Balduzzi, R. Puglisi, A. Galli, and P. Ferraro, Opt. Lett. 37, 4212 (2012).
[CrossRef]

M. Paturzo, A. Finizio, P. Memmolo, R. Puglisi, D. Balduzzi, A. Galli, and P. Ferraro, Lab Chip 12, 3073 (2012).
[CrossRef]

Forget, B. C.

Galli, A.

M. Paturzo, A. Finizio, P. Memmolo, R. Puglisi, D. Balduzzi, A. Galli, and P. Ferraro, Lab Chip 12, 3073 (2012).
[CrossRef]

V. Bianco, M. Paturzo, A. Finizio, D. Balduzzi, R. Puglisi, A. Galli, and P. Ferraro, Opt. Lett. 37, 4212 (2012).
[CrossRef]

Gong, H.

H. Cheng, Q. Luo, Q. Liu, Q. Lu, H. Gong, and S. Zeng, Phys. Med. Biol. 49, 1347 (2004).
[CrossRef]

H. Cheng, Q. Luo, Z. Wang, H. Gong, S. Chen, and W. Liang, Appl. Opt. 42, 5759 (2003).
[CrossRef]

Gross, M.

Harazny, J.

G. Michelson, J. Welzenbach, I. Pal, and J. Harazny, Br. J. Ophthalmol. 82, 1294 (1998).
[CrossRef]

Henderson, M.

Hondebrink, E.

Kang, N. M.

B. Choi, N. M. Kang, and S. Nelson, Microvasc. Res. 68, 143 (2004).
[CrossRef]

Lasser, T.

Leeuwen, T.

Li, P.

Liang, W.

Liu, Q.

H. Cheng, Q. Luo, Q. Liu, Q. Lu, H. Gong, and S. Zeng, Phys. Med. Biol. 49, 1347 (2004).
[CrossRef]

Lu, Q.

H. Cheng, Q. Luo, Q. Liu, Q. Lu, H. Gong, and S. Zeng, Phys. Med. Biol. 49, 1347 (2004).
[CrossRef]

Luo, Q.

Memmolo, P.

M. Paturzo, A. Finizio, P. Memmolo, R. Puglisi, D. Balduzzi, A. Galli, and P. Ferraro, Lab Chip 12, 3073 (2012).
[CrossRef]

Michelson, G.

G. Michelson, J. Welzenbach, I. Pal, and J. Harazny, Br. J. Ophthalmol. 82, 1294 (1998).
[CrossRef]

Moskowitz, M. A.

A. K. Dunn, H. Bolay, M. A. Moskowitz, and D. A. Boas, J. Cereb. Blood Flow Metab. 21, 195 (2001).
[CrossRef]

Moyer, A.

Nelson, S.

B. Choi, N. M. Kang, and S. Nelson, Microvasc. Res. 68, 143 (2004).
[CrossRef]

Ni, S.

Pal, I.

G. Michelson, J. Welzenbach, I. Pal, and J. Harazny, Br. J. Ophthalmol. 82, 1294 (1998).
[CrossRef]

Paturzo, M.

V. Bianco, M. Paturzo, A. Finizio, D. Balduzzi, R. Puglisi, A. Galli, and P. Ferraro, Opt. Lett. 37, 4212 (2012).
[CrossRef]

M. Paturzo, A. Finizio, P. Memmolo, R. Puglisi, D. Balduzzi, A. Galli, and P. Ferraro, Lab Chip 12, 3073 (2012).
[CrossRef]

Puglisi, R.

M. Paturzo, A. Finizio, P. Memmolo, R. Puglisi, D. Balduzzi, A. Galli, and P. Ferraro, Lab Chip 12, 3073 (2012).
[CrossRef]

V. Bianco, M. Paturzo, A. Finizio, D. Balduzzi, R. Puglisi, A. Galli, and P. Ferraro, Opt. Lett. 37, 4212 (2012).
[CrossRef]

Rancillac, A.

Serov, A.

Steenbergen, W.

Steinacher, B.

Vitalis, T.

Wang, D.

Wang, Z.

Webster, S.

J. D. Briers and S. Webster, Opt. Commun. 116, 36 (1995).
[CrossRef]

Welzenbach, J.

G. Michelson, J. Welzenbach, I. Pal, and J. Harazny, Br. J. Ophthalmol. 82, 1294 (1998).
[CrossRef]

Yan, Y.

Yuan, S.

Zeng, S.

P. Li, S. Ni, L. Zhang, S. Zeng, and Q. Luo, Opt. Lett. 31, 1824 (2006).
[CrossRef]

H. Cheng, Q. Luo, Q. Liu, Q. Lu, H. Gong, and S. Zeng, Phys. Med. Biol. 49, 1347 (2004).
[CrossRef]

Zhang, L.

Appl. Opt.

Br. J. Ophthalmol.

G. Michelson, J. Welzenbach, I. Pal, and J. Harazny, Br. J. Ophthalmol. 82, 1294 (1998).
[CrossRef]

J. Cereb. Blood Flow Metab.

A. K. Dunn, H. Bolay, M. A. Moskowitz, and D. A. Boas, J. Cereb. Blood Flow Metab. 21, 195 (2001).
[CrossRef]

Lab Chip

M. Paturzo, A. Finizio, P. Memmolo, R. Puglisi, D. Balduzzi, A. Galli, and P. Ferraro, Lab Chip 12, 3073 (2012).
[CrossRef]

Microvasc. Res.

B. Choi, N. M. Kang, and S. Nelson, Microvasc. Res. 68, 143 (2004).
[CrossRef]

Opt. Commun.

J. D. Briers and S. Webster, Opt. Commun. 116, 36 (1995).
[CrossRef]

Opt. Express

Opt. Lett.

Phys. Med. Biol.

H. Cheng, Q. Luo, Q. Liu, Q. Lu, H. Gong, and S. Zeng, Phys. Med. Biol. 49, 1347 (2004).
[CrossRef]

Rev. Sci. Instrum.

M. Atlan and M. Gross, Rev. Sci. Instrum. 77, 116103 (2006).
[CrossRef]

Cited By

OSA participates in CrossRef's Cited-By Linking service. Citing articles from OSA journals and other participating publishers are listed here.

Alert me when this article is cited.


Figures (4)

Fig. 1.
Fig. 1.

Schematic diagram of the IFM experimental setup.

Fig. 2.
Fig. 2.

(a) MD and (b) temporal speckle contrast via the velocity of a moving porcelain plate.

Fig. 3.
Fig. 3.

(a) IFM image and (b) temporal speckle contrast image. Curves of the (c) MD and (d) the temporal speckle contrast along the longitudinal dashed lines in (a) and (b).

Fig. 4.
Fig. 4.

(a) Flow images of an in vivo mouse pinna obtain by (a) IFM and (b) TSCA. Profiles of the (c) MD and (d) contrast along the horizontal dashed lines in (a) and (b).

Equations (1)

Equations on this page are rendered with MathJax. Learn more.

M(x,y)=ID(x,y)/IS(x,y),

Metrics