Abstract

We present a fast and flexible fluorescence lifetime imaging microscopy which uses a two-dimensional acousto-optic deflector to achieve fast beam scanning across the sample and provides random access to the regions of interests (ROI). Experimental results using standard fluorescent dye and biological samples show that this system can make addressable fluorescence lifetime measurements and perform fast and flexible fluorescence lifetime imaging particularly to the discontinuous ROI in the sample.

© 2013 Optical Society of America

Full Article  |  PDF Article
OSA Recommended Articles
Digital micromirror device as a spatial illuminator for fluorescence lifetime and hyperspectral imaging

Artur Bednarkiewicz, Mounir Bouhifd, and Maurice P. Whelan
Appl. Opt. 47(9) 1193-1199 (2008)

Ultrafast, large-field multiphoton microscopy based on an acousto-optic deflector and a spatial light modulator

Yonghong Shao, Wan Qin, Honghai Liu, Junle Qu, Xiang Peng, Hanben Niu, and Bruce Z. Gao
Opt. Lett. 37(13) 2532-2534 (2012)

Fast two-dimensional standing-wave total-internal-reflection fluorescence microscopy using acousto-optic deflectors

Olga Gliko, William E. Brownell, and Peter Saggau
Opt. Lett. 34(6) 836-838 (2009)

References

You do not have subscription access to this journal. Citation lists with outbound citation links are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription

Cited By

You do not have subscription access to this journal. Cited by links are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription

Figures (4)

You do not have subscription access to this journal. Figure files are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription

Metrics

You do not have subscription access to this journal. Article level metrics are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription