Abstract

We present an algorithm to estimate the location of single fluorescent molecule with both high speed and high precision. This algorithm is based on finding the subpixel position with maximum radial symmetry in a pixelated single molecule fluorescence image. Compared with conventional algorithms, this algorithm does not rely on point-spread-function or noise model. Through numerical simulation and experimental analysis, we found that this algorithm exhibits localization precision very close to the maximum likelihood estimator (MLE), while executes ∼1000 times faster than the MLE and ∼6 times faster than the fluoroBancroft algorithm.

© 2012 Optical Society of America

PDF Article

References

  • View by:
  • |
  • |
  • |

  1. B. Huang, H. Babcock, and X. W. Zhuang, Cell 143, 1047 (2010).
    [CrossRef]
  2. R. J. Ober, S. Ram, and E. S. Ward, Biophys. J. 86, 1185 (2004).
    [CrossRef]
  3. Z. L. Shen and S. B. Andersson, IEEE Trans. Signal Process. 59, 4041 (2011).
    [CrossRef]
  4. B. Yu, D. N. Chen, J. L. Qu, and H. B. Niu, Opt. Lett. 36, 4317 (2011).
    [CrossRef]
  5. R. Starr, S. Stahlheber, and A. Small, Opt. Lett. 37, 413 (2012).
    [CrossRef]
  6. T. W. Quan, P. C. Li, F. Long, S. Q. Zeng, Q. M. Luo, P. N. Hedde, G. U. Nienhaus, and Z. L. Huang, Opt. Express 18, 11867 (2010).
    [CrossRef]
  7. C. S. Smith, N. Joseph, B. Rieger, and K. A. Lidke, Nat. Meth. 7, 373 (2010).
    [CrossRef]
  8. Z. L. Huang, H. Y. Zhu, F. Long, H. Q. Ma, L. S. Qin, Y. F. Liu, J. P. Ding, Z. H. Zhang, Q. M. Luo, and S. Q. Zeng, Opt. Express 19, 19156 (2011).
    [CrossRef]
  9. P. Friedl and D. Gilmour, Nat. Rev. Mol. Cell Biol. 10, 445 (2009).
    [CrossRef]
  10. J. B. Pawley, Handbook of Biological Confocal Microscopy, 3rd ed. (Springer, 2006).
  11. P. N. Hedde, J. Fuchs, F. Oswald, J. Wiedenmann, and G. U. Nienhaus, Nat. Meth. 6, 689 (2009).
    [CrossRef]
  12. K. Nienhaus, G. U. Nienhaus, J. Wiedenmann, and H. Nar, Proc. Natl. Acad. Sci. USA 102, 9156 (2005).
    [CrossRef]

2012

2011

2010

B. Huang, H. Babcock, and X. W. Zhuang, Cell 143, 1047 (2010).
[CrossRef]

C. S. Smith, N. Joseph, B. Rieger, and K. A. Lidke, Nat. Meth. 7, 373 (2010).
[CrossRef]

T. W. Quan, P. C. Li, F. Long, S. Q. Zeng, Q. M. Luo, P. N. Hedde, G. U. Nienhaus, and Z. L. Huang, Opt. Express 18, 11867 (2010).
[CrossRef]

2009

P. Friedl and D. Gilmour, Nat. Rev. Mol. Cell Biol. 10, 445 (2009).
[CrossRef]

P. N. Hedde, J. Fuchs, F. Oswald, J. Wiedenmann, and G. U. Nienhaus, Nat. Meth. 6, 689 (2009).
[CrossRef]

2005

K. Nienhaus, G. U. Nienhaus, J. Wiedenmann, and H. Nar, Proc. Natl. Acad. Sci. USA 102, 9156 (2005).
[CrossRef]

2004

R. J. Ober, S. Ram, and E. S. Ward, Biophys. J. 86, 1185 (2004).
[CrossRef]

Andersson, S. B.

Z. L. Shen and S. B. Andersson, IEEE Trans. Signal Process. 59, 4041 (2011).
[CrossRef]

Babcock, H.

B. Huang, H. Babcock, and X. W. Zhuang, Cell 143, 1047 (2010).
[CrossRef]

Chen, D. N.

Ding, J. P.

Friedl, P.

P. Friedl and D. Gilmour, Nat. Rev. Mol. Cell Biol. 10, 445 (2009).
[CrossRef]

Fuchs, J.

P. N. Hedde, J. Fuchs, F. Oswald, J. Wiedenmann, and G. U. Nienhaus, Nat. Meth. 6, 689 (2009).
[CrossRef]

Gilmour, D.

P. Friedl and D. Gilmour, Nat. Rev. Mol. Cell Biol. 10, 445 (2009).
[CrossRef]

Hedde, P. N.

Huang, B.

B. Huang, H. Babcock, and X. W. Zhuang, Cell 143, 1047 (2010).
[CrossRef]

Huang, Z. L.

Joseph, N.

C. S. Smith, N. Joseph, B. Rieger, and K. A. Lidke, Nat. Meth. 7, 373 (2010).
[CrossRef]

Li, P. C.

Lidke, K. A.

C. S. Smith, N. Joseph, B. Rieger, and K. A. Lidke, Nat. Meth. 7, 373 (2010).
[CrossRef]

Liu, Y. F.

Long, F.

Luo, Q. M.

Ma, H. Q.

Nar, H.

K. Nienhaus, G. U. Nienhaus, J. Wiedenmann, and H. Nar, Proc. Natl. Acad. Sci. USA 102, 9156 (2005).
[CrossRef]

Nienhaus, G. U.

T. W. Quan, P. C. Li, F. Long, S. Q. Zeng, Q. M. Luo, P. N. Hedde, G. U. Nienhaus, and Z. L. Huang, Opt. Express 18, 11867 (2010).
[CrossRef]

P. N. Hedde, J. Fuchs, F. Oswald, J. Wiedenmann, and G. U. Nienhaus, Nat. Meth. 6, 689 (2009).
[CrossRef]

K. Nienhaus, G. U. Nienhaus, J. Wiedenmann, and H. Nar, Proc. Natl. Acad. Sci. USA 102, 9156 (2005).
[CrossRef]

Nienhaus, K.

K. Nienhaus, G. U. Nienhaus, J. Wiedenmann, and H. Nar, Proc. Natl. Acad. Sci. USA 102, 9156 (2005).
[CrossRef]

Niu, H. B.

Ober, R. J.

R. J. Ober, S. Ram, and E. S. Ward, Biophys. J. 86, 1185 (2004).
[CrossRef]

Oswald, F.

P. N. Hedde, J. Fuchs, F. Oswald, J. Wiedenmann, and G. U. Nienhaus, Nat. Meth. 6, 689 (2009).
[CrossRef]

Pawley, J. B.

J. B. Pawley, Handbook of Biological Confocal Microscopy, 3rd ed. (Springer, 2006).

Qin, L. S.

Qu, J. L.

Quan, T. W.

Ram, S.

R. J. Ober, S. Ram, and E. S. Ward, Biophys. J. 86, 1185 (2004).
[CrossRef]

Rieger, B.

C. S. Smith, N. Joseph, B. Rieger, and K. A. Lidke, Nat. Meth. 7, 373 (2010).
[CrossRef]

Shen, Z. L.

Z. L. Shen and S. B. Andersson, IEEE Trans. Signal Process. 59, 4041 (2011).
[CrossRef]

Small, A.

Smith, C. S.

C. S. Smith, N. Joseph, B. Rieger, and K. A. Lidke, Nat. Meth. 7, 373 (2010).
[CrossRef]

Stahlheber, S.

Starr, R.

Ward, E. S.

R. J. Ober, S. Ram, and E. S. Ward, Biophys. J. 86, 1185 (2004).
[CrossRef]

Wiedenmann, J.

P. N. Hedde, J. Fuchs, F. Oswald, J. Wiedenmann, and G. U. Nienhaus, Nat. Meth. 6, 689 (2009).
[CrossRef]

K. Nienhaus, G. U. Nienhaus, J. Wiedenmann, and H. Nar, Proc. Natl. Acad. Sci. USA 102, 9156 (2005).
[CrossRef]

Yu, B.

Zeng, S. Q.

Zhang, Z. H.

Zhu, H. Y.

Zhuang, X. W.

B. Huang, H. Babcock, and X. W. Zhuang, Cell 143, 1047 (2010).
[CrossRef]

Biophys. J.

R. J. Ober, S. Ram, and E. S. Ward, Biophys. J. 86, 1185 (2004).
[CrossRef]

Cell

B. Huang, H. Babcock, and X. W. Zhuang, Cell 143, 1047 (2010).
[CrossRef]

IEEE Trans. Signal Process.

Z. L. Shen and S. B. Andersson, IEEE Trans. Signal Process. 59, 4041 (2011).
[CrossRef]

Nat. Meth.

C. S. Smith, N. Joseph, B. Rieger, and K. A. Lidke, Nat. Meth. 7, 373 (2010).
[CrossRef]

P. N. Hedde, J. Fuchs, F. Oswald, J. Wiedenmann, and G. U. Nienhaus, Nat. Meth. 6, 689 (2009).
[CrossRef]

Nat. Rev. Mol. Cell Biol.

P. Friedl and D. Gilmour, Nat. Rev. Mol. Cell Biol. 10, 445 (2009).
[CrossRef]

Opt. Express

Opt. Lett.

Proc. Natl. Acad. Sci. USA

K. Nienhaus, G. U. Nienhaus, J. Wiedenmann, and H. Nar, Proc. Natl. Acad. Sci. USA 102, 9156 (2005).
[CrossRef]

Other

J. B. Pawley, Handbook of Biological Confocal Microscopy, 3rd ed. (Springer, 2006).

Cited By

OSA participates in CrossRef's Cited-By Linking service. Citing articles from OSA journals and other participating publishers are listed here.

Alert me when this article is cited.


Metrics