Abstract

Optical microcavities are high sensitivity transducers able to detect single nanoparticles and molecules. However, the specificity of detection is dependent on the availability of an appropriate targeting moiety with minimal cross-reactivity. In the present work, an alternative approach is shown. Namely, using biotin-functionalized toroidal microcavities, the dissociation constant of biotin to two different streptavidin complexes (free and polystyrene bead) is determined. Based on the difference in affinity and in mass transport, the two complexes are identified from a mixture. By leveraging information in the binding site, improved specificity can be achieved.

© 2012 Optical Society of America

Full Article  |  PDF Article
OSA Recommended Articles
Protein detection on biotin-derivatized polyallylamine by optical microring resonators

Daniela Ullien, Peter J. Harmsma, Shahina M. C. Abdulla, Bart M. de Boer, Duco Bosma, Ernst J. R. Sudhölter, Louis C. P. M. de Smet, and Wolter F. Jager
Opt. Express 22(13) 16585-16594 (2014)

Protein biosensing with fluorescent microcapillaries

S. Lane, P. West, A. François, and A. Meldrum
Opt. Express 23(3) 2577-2590 (2015)

Whispering gallery mode sensors

Matthew R. Foreman, Jon D. Swaim, and Frank Vollmer
Adv. Opt. Photon. 7(2) 168-240 (2015)

References

You do not have subscription access to this journal. Citation lists with outbound citation links are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription

Cited By

You do not have subscription access to this journal. Cited by links are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription

Figures (3)

You do not have subscription access to this journal. Figure files are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription

Equations (1)

You do not have subscription access to this journal. Equations are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription

Metrics

You do not have subscription access to this journal. Article level metrics are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription