Abstract

We present single-exposure super-resolved interferometric microscopy (SESRIM) as a novel approach capable of providing one-dimensional (1-D) super-resolution (SR) imaging in holographic microscopy using a single illumination shot. The single-exposure SR working principle is achieved by combining angular and wavelength multiplexing incoming from a set of tilted beams with different wavelengths where each wavelength is tuned with the red–green–blue (RGB) channels of a color CCD. Thus, the information included in each color channel is retrieved by holographic recording using a single-color CCD capture and by analyzing the RGB channels. Finally, 1-D SR imaging is obtained after the digital postprocessing stage yielding the generation of a synthetic aperture. Experimental re sults are reported validating the proposed SESRIM approach while an extension of the proposed approach to the two-dimensional case is considered.

© 2011 Optical Society of America

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References

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  19. T. Kreis, Handbook of Holographic Interferometry: Optical and Digital Methods (Wiley-VCH, 2005).

2011 (1)

2010 (4)

V. Mico and Z. Zalevsky, J. Biomed. Opt. 15, 046027 (2010).
[CrossRef] [PubMed]

L. Granero, V. Micó, Z. Zalevsky, and J. García, Appl. Opt. 49, 845 (2010).
[CrossRef] [PubMed]

J. Zhao, X. Yan, W. Sun, and J. Di, Opt. Lett. 35, 3519 (2010).
[CrossRef] [PubMed]

J. Bühl, H. Babovsky, A. Kiessling, and R. Kowarschik, Opt. Commun. 283, 3631 (2010).
[CrossRef]

2008 (3)

2007 (1)

2006 (3)

2004 (1)

2003 (1)

1974 (1)

1971 (1)

1873 (1)

E. Abbe, Archiv. Mikr. Anat. 9, 413 (1873).
[CrossRef]

Abbe, E.

E. Abbe, Archiv. Mikr. Anat. 9, 413 (1873).
[CrossRef]

Alexandrov, S. A.

S. A. Alexandrov, T. R. Hillman, T. Gutzler, and D. D. Sampson, Phys. Rev. Lett. 97, 168102 (2006).
[CrossRef] [PubMed]

Babovsky, H.

J. Bühl, H. Babovsky, A. Kiessling, and R. Kowarschik, Opt. Commun. 283, 3631 (2010).
[CrossRef]

Brueck, S. R. J.

Bühl, J.

J. Bühl, H. Babovsky, A. Kiessling, and R. Kowarschik, Opt. Commun. 283, 3631 (2010).
[CrossRef]

Di, J.

Ferreira, C.

García, J.

García-Martínez, P.

Granero, L.

Gutzler, T.

S. A. Alexandrov, T. R. Hillman, T. Gutzler, and D. D. Sampson, Phys. Rev. Lett. 97, 168102 (2006).
[CrossRef] [PubMed]

Hillman, T. R.

S. A. Alexandrov, T. R. Hillman, T. Gutzler, and D. D. Sampson, Phys. Rev. Lett. 97, 168102 (2006).
[CrossRef] [PubMed]

Kiessling, A.

J. Bühl, H. Babovsky, A. Kiessling, and R. Kowarschik, Opt. Commun. 283, 3631 (2010).
[CrossRef]

Kowarschik, R.

J. Bühl, H. Babovsky, A. Kiessling, and R. Kowarschik, Opt. Commun. 283, 3631 (2010).
[CrossRef]

Kreis, T.

T. Kreis, Handbook of Holographic Interferometry: Optical and Digital Methods (Wiley-VCH, 2005).

Kuznetsova, Y.

Liu, H.

Ma, J.

Mendlovic, D.

Z. Zalevsky and D. Mendlovic, Optical Super Resolution (Springer, 2002).

Mico, V.

Micó, V.

Neumann, A.

Osten, W.

Pedrini, G.

Sampson, D. D.

S. A. Alexandrov, T. R. Hillman, T. Gutzler, and D. D. Sampson, Phys. Rev. Lett. 97, 168102 (2006).
[CrossRef] [PubMed]

Sato, T.

Schwarz, C. J.

Situ, G.

Sun, W.

Ueda, M.

Yamagishi, G.

Yan, X.

Yuan, C.

Zalevsky, Z.

Zhai, H.

Zhao, J.

Appl. Opt. (3)

Archiv. Mikr. Anat. (1)

E. Abbe, Archiv. Mikr. Anat. 9, 413 (1873).
[CrossRef]

J. Biomed. Opt. (1)

V. Mico and Z. Zalevsky, J. Biomed. Opt. 15, 046027 (2010).
[CrossRef] [PubMed]

J. Opt. Soc. Am. (1)

J. Opt. Soc. Am. A (1)

Opt. Commun. (2)

V. Mico, Z. Zalevsky, and J. García, Opt. Commun. 281, 4273 (2008).
[CrossRef]

J. Bühl, H. Babovsky, A. Kiessling, and R. Kowarschik, Opt. Commun. 283, 3631 (2010).
[CrossRef]

Opt. Express (4)

Opt. Lett. (3)

Phys. Rev. Lett. (1)

S. A. Alexandrov, T. R. Hillman, T. Gutzler, and D. D. Sampson, Phys. Rev. Lett. 97, 168102 (2006).
[CrossRef] [PubMed]

Other (2)

Z. Zalevsky and D. Mendlovic, Optical Super Resolution (Springer, 2002).

T. Kreis, Handbook of Holographic Interferometry: Optical and Digital Methods (Wiley-VCH, 2005).

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Figures (4)

Fig. 1
Fig. 1

Experimental setup for SESRIM validation where BS is a beam splitter cube.

Fig. 2
Fig. 2

Schematic chart of the SESRIM approach.

Fig. 3
Fig. 3

Experimental results using a swine sperm biosample: (a) the RGB holograms, (b) the conventional image, (c) the SR image. Scale bars in cases (b) and (c) are 10 μm .

Fig. 4
Fig. 4

Two-dimensional extension of the proposed method by using time multiplexing: (a), (b) the horizontal and vertical RGB holograms, respectively, (c) the conventional low-resolution image, (d) the generated SA, and (e), (f) the super-resolved images in 1-D and 2-D, respectively. The insets show magnified regions of the images to clearly visualize the SR effect.

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