Abstract

We present a fiber-optic low-coherence imaging technique, termed spectral-domain differential interference contrast microscopy (SD-DIC), for quantitative DIC imaging of both reflective surfaces and transparent biological specimens. SD-DIC combines the common-path nature of a Nomarski DIC interferometer with the high sensitivity of spectral-domain low-coherence interferometry to obtain high-resolution, quantitative measurements of optical pathlength gradients from a single point on the sample. Full-field imaging can be achieved by scanning the sample. A reflected-light SD-DIC system was demonstrated using a USAF resolution target as the phase object. Live cardiomyocytes were also imaged, achieving a resolution of 36pm for pathlength gradient measurements. The dynamics of cardiomyocyte contraction were recorded with high sensitivity at selected sites on the cells.

© 2011 Optical Society of America

Full Article  |  PDF Article
OSA Recommended Articles
Automated multi-parameter measurement of cardiomyocytes dynamics with digital holographic microscopy

Benjamin Rappaz, Inkyu Moon, Faliu Yi, Bahram Javidi, Pierre Marquet, and Gerardo Turcatti
Opt. Express 23(10) 13333-13347 (2015)

Gradient field microscopy of unstained specimens

Taewoo Kim, Shamira Sridharan, and Gabriel Popescu
Opt. Express 20(6) 6737-6745 (2012)

Dynamic DIC by digital holography microscopy for enhancing phase-contrast visualization

Lisa Miccio, Andrea Finizio, Roberto Puglisi, Donatella Balduzzi, Andrea Galli, and Pietro Ferraro
Biomed. Opt. Express 2(2) 331-344 (2011)

References

You do not have subscription access to this journal. Citation lists with outbound citation links are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription

Cited By

You do not have subscription access to this journal. Cited by links are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription

Figures (3)

You do not have subscription access to this journal. Figure files are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription

Equations (1)

You do not have subscription access to this journal. Equations are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription

Metrics

You do not have subscription access to this journal. Article level metrics are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription