Abstract

Nonlinear phase dispersion spectroscopy is introduced as a means to retrieve wideband, high spectral resolution profiles of the wavelength-dependent real part of the refractive index. The method is based on detecting dispersion effects imparted to a light field with low coherence transmitted through a thin sample and detected interferometrically in the spectral domain. The same sampled signal is also processed to yield quantitative phase maps and spectral information regarding the total attenuation coefficient using spectral-domain phase microscopy and spectroscopic optical coherence tomography (SOCT), respectively. Proof-of-concept experiments using fluorescent and nonfluorescent polystyrene beads and another using a red blood cell demonstrate the ability of the method to quantify various absorptive/dispersive features. The increased sensitivity of this method, novel to our knowledge, is compared to intensity-based spectroscopy (e.g., SOCT), and potential applications are discussed.

© 2011 Optical Society of America

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References

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2011 (3)

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[CrossRef] [PubMed]

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[CrossRef]

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[CrossRef] [PubMed]

2010 (4)

2009 (3)

2008 (3)

2005 (1)

2000 (2)

1971 (1)

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Chowdhury, S.

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[CrossRef] [PubMed]

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Drexler, W.

Ellerbee, A.

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Feld, M. S.

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[CrossRef] [PubMed]

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[CrossRef] [PubMed]

Suresh, S.

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[CrossRef] [PubMed]

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[CrossRef] [PubMed]

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[CrossRef] [PubMed]

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Biomed. Opt. Express (1)

J. Biomed. Opt. (1)

N. T. Shaked, L. L. Satterwhite, M. J. Telen, G. A. Truskey, and A. Wax, J. Biomed. Opt. 16, 030506 (2011).
[CrossRef] [PubMed]

J. Opt. Soc. Am. (1)

Nat. Photon. (1)

F. E. Robles, C. Wilson, G. Grant, and A. Wax, Nat. Photon. , doi:10.1038/nphoton.2011.257 (2011).
[CrossRef]

Opt. Lett. (12)

Proc. Natl. Acad. Sci. USA (1)

Y. Park, M. Diez-Silva, G. Popescu, G. Lykotrafitis, W. Choi, M. S. Feld, and S. Suresh, Proc. Natl. Acad. Sci. USA 105, 13730 (2008).
[CrossRef] [PubMed]

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Figures (3)

Fig. 1
Fig. 1

(a) Unwrapped phase of an interferogram acquired from the center of the fluorescent bead and the line of best fit used to correct for dispersion effects. (b) Topological map of nonfluorescent (clear) and fluorescent (fluoro) beads.

Fig. 2
Fig. 2

(a) Negative log of the normalized spectrum from the center of each bead. (b) True color representation of the sample superposed on the topological map. (c) Changes in the RI for a point at the center of each bead. (d) Negative log of the standard deviation of the changes in the real part of the RI superposed with the topological map.

Fig. 3
Fig. 3

(a) Topological map of a healthy RBC. (b) True color representation superposed with the topological map. (c) Negative log of the standard deviation of the changes in the real part of the RI superposed with the topological map. (d) Representative spectral profile of the real part of the RI.

Equations (1)

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I INT ( ω ) e μ tot ( ω ) d · e i ( ω / c 0 ) · 2 d n 0 · e i ( ω / c 0 ) · 2 d Δ n ( ω ) ,

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