Abstract

Quantification of biomarkers using multispectral optoacoustic tomography can be challenging due to photon fluence variations with depth and spatially heterogeneous tissue optical properties. Herein we introduce a spectral ratio approach that accounts for photon fluence variations. The performance and imaging improvement achieved with the proposed method is showcased both numerically and experimentally in phantoms and mice.

© 2011 Optical Society of America

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References

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  1. X. Wang, Y. Pang, G. Ku, X. Xie, G. Stoica, and L. V. Wang, Nat. Biotechnol. 21, 803 (2003).
    [CrossRef] [PubMed]
  2. D. Razansky, M. Distel, C. Vinegoni, R. Ma, N. Perrimon, R. W. Koster, and V. Ntziachristos, Nat. Photon. 3, 412(2009).
    [CrossRef]
  3. D. Razansky, C. Vinegoni, and V. Ntziachristos, Opt. Lett. 32, 2891 (2007).
    [CrossRef] [PubMed]
  4. B. T. Cox, S. R. Arridge, and P. C. Beard, J. Opt. Soc. Am. A 26, 443 (2009).
    [CrossRef]
  5. T. Jetzfellner, D. Razansky, A. Rosenthal, R. Schulz, K. H. Englmeier, and V. Ntziachristos, Appl. Phys. Lett. 95, 013703 (2009).
    [CrossRef]
  6. A. Rosenthal, D. Razansky, and V. Ntziachristos, IEEE Trans. Med. Imaging 28, 1997 (2009).
    [CrossRef] [PubMed]
  7. V. Ntziachristos, C.-H. Tung, C. Bremer, and R. Weissleder, Nat. Med. 8, 757 (2002).
    [CrossRef] [PubMed]
  8. A. Rosenthal, D. Razansky, and V. Ntziachristos, IEEE Trans. Med. Imaging 29, 1275 (2010).
    [CrossRef] [PubMed]
  9. M. Xu and L. V. Wang, Phys. Rev. E 71, 016706 (2005).
    [CrossRef]
  10. T. Jetzfellner, A. Rosenthal, K. H. Englmeier, A. Dima, M. Á. A. Caballero, D. Razansky, and V. Ntziachristos, Appl. Phys. Lett. 98, 163701 (2011).
    [CrossRef]
  11. A. Buehler, E. Herzog, D. Razansky, and V. Ntziachristos, Opt. Lett. 35, 2475 (2010).
    [CrossRef] [PubMed]

2011 (1)

T. Jetzfellner, A. Rosenthal, K. H. Englmeier, A. Dima, M. Á. A. Caballero, D. Razansky, and V. Ntziachristos, Appl. Phys. Lett. 98, 163701 (2011).
[CrossRef]

2010 (2)

A. Buehler, E. Herzog, D. Razansky, and V. Ntziachristos, Opt. Lett. 35, 2475 (2010).
[CrossRef] [PubMed]

A. Rosenthal, D. Razansky, and V. Ntziachristos, IEEE Trans. Med. Imaging 29, 1275 (2010).
[CrossRef] [PubMed]

2009 (4)

D. Razansky, M. Distel, C. Vinegoni, R. Ma, N. Perrimon, R. W. Koster, and V. Ntziachristos, Nat. Photon. 3, 412(2009).
[CrossRef]

B. T. Cox, S. R. Arridge, and P. C. Beard, J. Opt. Soc. Am. A 26, 443 (2009).
[CrossRef]

T. Jetzfellner, D. Razansky, A. Rosenthal, R. Schulz, K. H. Englmeier, and V. Ntziachristos, Appl. Phys. Lett. 95, 013703 (2009).
[CrossRef]

A. Rosenthal, D. Razansky, and V. Ntziachristos, IEEE Trans. Med. Imaging 28, 1997 (2009).
[CrossRef] [PubMed]

2007 (1)

2005 (1)

M. Xu and L. V. Wang, Phys. Rev. E 71, 016706 (2005).
[CrossRef]

2003 (1)

X. Wang, Y. Pang, G. Ku, X. Xie, G. Stoica, and L. V. Wang, Nat. Biotechnol. 21, 803 (2003).
[CrossRef] [PubMed]

2002 (1)

V. Ntziachristos, C.-H. Tung, C. Bremer, and R. Weissleder, Nat. Med. 8, 757 (2002).
[CrossRef] [PubMed]

Arridge, S. R.

Beard, P. C.

Bremer, C.

V. Ntziachristos, C.-H. Tung, C. Bremer, and R. Weissleder, Nat. Med. 8, 757 (2002).
[CrossRef] [PubMed]

Buehler, A.

Caballero, M. Á. A.

T. Jetzfellner, A. Rosenthal, K. H. Englmeier, A. Dima, M. Á. A. Caballero, D. Razansky, and V. Ntziachristos, Appl. Phys. Lett. 98, 163701 (2011).
[CrossRef]

Cox, B. T.

Dima, A.

T. Jetzfellner, A. Rosenthal, K. H. Englmeier, A. Dima, M. Á. A. Caballero, D. Razansky, and V. Ntziachristos, Appl. Phys. Lett. 98, 163701 (2011).
[CrossRef]

Distel, M.

D. Razansky, M. Distel, C. Vinegoni, R. Ma, N. Perrimon, R. W. Koster, and V. Ntziachristos, Nat. Photon. 3, 412(2009).
[CrossRef]

Englmeier, K. H.

T. Jetzfellner, A. Rosenthal, K. H. Englmeier, A. Dima, M. Á. A. Caballero, D. Razansky, and V. Ntziachristos, Appl. Phys. Lett. 98, 163701 (2011).
[CrossRef]

T. Jetzfellner, D. Razansky, A. Rosenthal, R. Schulz, K. H. Englmeier, and V. Ntziachristos, Appl. Phys. Lett. 95, 013703 (2009).
[CrossRef]

Herzog, E.

Jetzfellner, T.

T. Jetzfellner, A. Rosenthal, K. H. Englmeier, A. Dima, M. Á. A. Caballero, D. Razansky, and V. Ntziachristos, Appl. Phys. Lett. 98, 163701 (2011).
[CrossRef]

T. Jetzfellner, D. Razansky, A. Rosenthal, R. Schulz, K. H. Englmeier, and V. Ntziachristos, Appl. Phys. Lett. 95, 013703 (2009).
[CrossRef]

Koster, R. W.

D. Razansky, M. Distel, C. Vinegoni, R. Ma, N. Perrimon, R. W. Koster, and V. Ntziachristos, Nat. Photon. 3, 412(2009).
[CrossRef]

Ku, G.

X. Wang, Y. Pang, G. Ku, X. Xie, G. Stoica, and L. V. Wang, Nat. Biotechnol. 21, 803 (2003).
[CrossRef] [PubMed]

Ma, R.

D. Razansky, M. Distel, C. Vinegoni, R. Ma, N. Perrimon, R. W. Koster, and V. Ntziachristos, Nat. Photon. 3, 412(2009).
[CrossRef]

Ntziachristos, V.

T. Jetzfellner, A. Rosenthal, K. H. Englmeier, A. Dima, M. Á. A. Caballero, D. Razansky, and V. Ntziachristos, Appl. Phys. Lett. 98, 163701 (2011).
[CrossRef]

A. Buehler, E. Herzog, D. Razansky, and V. Ntziachristos, Opt. Lett. 35, 2475 (2010).
[CrossRef] [PubMed]

A. Rosenthal, D. Razansky, and V. Ntziachristos, IEEE Trans. Med. Imaging 29, 1275 (2010).
[CrossRef] [PubMed]

A. Rosenthal, D. Razansky, and V. Ntziachristos, IEEE Trans. Med. Imaging 28, 1997 (2009).
[CrossRef] [PubMed]

T. Jetzfellner, D. Razansky, A. Rosenthal, R. Schulz, K. H. Englmeier, and V. Ntziachristos, Appl. Phys. Lett. 95, 013703 (2009).
[CrossRef]

D. Razansky, M. Distel, C. Vinegoni, R. Ma, N. Perrimon, R. W. Koster, and V. Ntziachristos, Nat. Photon. 3, 412(2009).
[CrossRef]

D. Razansky, C. Vinegoni, and V. Ntziachristos, Opt. Lett. 32, 2891 (2007).
[CrossRef] [PubMed]

V. Ntziachristos, C.-H. Tung, C. Bremer, and R. Weissleder, Nat. Med. 8, 757 (2002).
[CrossRef] [PubMed]

Pang, Y.

X. Wang, Y. Pang, G. Ku, X. Xie, G. Stoica, and L. V. Wang, Nat. Biotechnol. 21, 803 (2003).
[CrossRef] [PubMed]

Perrimon, N.

D. Razansky, M. Distel, C. Vinegoni, R. Ma, N. Perrimon, R. W. Koster, and V. Ntziachristos, Nat. Photon. 3, 412(2009).
[CrossRef]

Razansky, D.

T. Jetzfellner, A. Rosenthal, K. H. Englmeier, A. Dima, M. Á. A. Caballero, D. Razansky, and V. Ntziachristos, Appl. Phys. Lett. 98, 163701 (2011).
[CrossRef]

A. Buehler, E. Herzog, D. Razansky, and V. Ntziachristos, Opt. Lett. 35, 2475 (2010).
[CrossRef] [PubMed]

A. Rosenthal, D. Razansky, and V. Ntziachristos, IEEE Trans. Med. Imaging 29, 1275 (2010).
[CrossRef] [PubMed]

A. Rosenthal, D. Razansky, and V. Ntziachristos, IEEE Trans. Med. Imaging 28, 1997 (2009).
[CrossRef] [PubMed]

T. Jetzfellner, D. Razansky, A. Rosenthal, R. Schulz, K. H. Englmeier, and V. Ntziachristos, Appl. Phys. Lett. 95, 013703 (2009).
[CrossRef]

D. Razansky, M. Distel, C. Vinegoni, R. Ma, N. Perrimon, R. W. Koster, and V. Ntziachristos, Nat. Photon. 3, 412(2009).
[CrossRef]

D. Razansky, C. Vinegoni, and V. Ntziachristos, Opt. Lett. 32, 2891 (2007).
[CrossRef] [PubMed]

Rosenthal, A.

T. Jetzfellner, A. Rosenthal, K. H. Englmeier, A. Dima, M. Á. A. Caballero, D. Razansky, and V. Ntziachristos, Appl. Phys. Lett. 98, 163701 (2011).
[CrossRef]

A. Rosenthal, D. Razansky, and V. Ntziachristos, IEEE Trans. Med. Imaging 29, 1275 (2010).
[CrossRef] [PubMed]

T. Jetzfellner, D. Razansky, A. Rosenthal, R. Schulz, K. H. Englmeier, and V. Ntziachristos, Appl. Phys. Lett. 95, 013703 (2009).
[CrossRef]

A. Rosenthal, D. Razansky, and V. Ntziachristos, IEEE Trans. Med. Imaging 28, 1997 (2009).
[CrossRef] [PubMed]

Schulz, R.

T. Jetzfellner, D. Razansky, A. Rosenthal, R. Schulz, K. H. Englmeier, and V. Ntziachristos, Appl. Phys. Lett. 95, 013703 (2009).
[CrossRef]

Stoica, G.

X. Wang, Y. Pang, G. Ku, X. Xie, G. Stoica, and L. V. Wang, Nat. Biotechnol. 21, 803 (2003).
[CrossRef] [PubMed]

Tung, C.-H.

V. Ntziachristos, C.-H. Tung, C. Bremer, and R. Weissleder, Nat. Med. 8, 757 (2002).
[CrossRef] [PubMed]

Vinegoni, C.

D. Razansky, M. Distel, C. Vinegoni, R. Ma, N. Perrimon, R. W. Koster, and V. Ntziachristos, Nat. Photon. 3, 412(2009).
[CrossRef]

D. Razansky, C. Vinegoni, and V. Ntziachristos, Opt. Lett. 32, 2891 (2007).
[CrossRef] [PubMed]

Wang, L. V.

M. Xu and L. V. Wang, Phys. Rev. E 71, 016706 (2005).
[CrossRef]

X. Wang, Y. Pang, G. Ku, X. Xie, G. Stoica, and L. V. Wang, Nat. Biotechnol. 21, 803 (2003).
[CrossRef] [PubMed]

Wang, X.

X. Wang, Y. Pang, G. Ku, X. Xie, G. Stoica, and L. V. Wang, Nat. Biotechnol. 21, 803 (2003).
[CrossRef] [PubMed]

Weissleder, R.

V. Ntziachristos, C.-H. Tung, C. Bremer, and R. Weissleder, Nat. Med. 8, 757 (2002).
[CrossRef] [PubMed]

Xie, X.

X. Wang, Y. Pang, G. Ku, X. Xie, G. Stoica, and L. V. Wang, Nat. Biotechnol. 21, 803 (2003).
[CrossRef] [PubMed]

Xu, M.

M. Xu and L. V. Wang, Phys. Rev. E 71, 016706 (2005).
[CrossRef]

Appl. Phys. Lett. (2)

T. Jetzfellner, D. Razansky, A. Rosenthal, R. Schulz, K. H. Englmeier, and V. Ntziachristos, Appl. Phys. Lett. 95, 013703 (2009).
[CrossRef]

T. Jetzfellner, A. Rosenthal, K. H. Englmeier, A. Dima, M. Á. A. Caballero, D. Razansky, and V. Ntziachristos, Appl. Phys. Lett. 98, 163701 (2011).
[CrossRef]

IEEE Trans. Med. Imaging (2)

A. Rosenthal, D. Razansky, and V. Ntziachristos, IEEE Trans. Med. Imaging 29, 1275 (2010).
[CrossRef] [PubMed]

A. Rosenthal, D. Razansky, and V. Ntziachristos, IEEE Trans. Med. Imaging 28, 1997 (2009).
[CrossRef] [PubMed]

J. Opt. Soc. Am. A (1)

Nat. Biotechnol. (1)

X. Wang, Y. Pang, G. Ku, X. Xie, G. Stoica, and L. V. Wang, Nat. Biotechnol. 21, 803 (2003).
[CrossRef] [PubMed]

Nat. Med. (1)

V. Ntziachristos, C.-H. Tung, C. Bremer, and R. Weissleder, Nat. Med. 8, 757 (2002).
[CrossRef] [PubMed]

Nat. Photon. (1)

D. Razansky, M. Distel, C. Vinegoni, R. Ma, N. Perrimon, R. W. Koster, and V. Ntziachristos, Nat. Photon. 3, 412(2009).
[CrossRef]

Opt. Lett. (2)

Phys. Rev. E (1)

M. Xu and L. V. Wang, Phys. Rev. E 71, 016706 (2005).
[CrossRef]

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Figures (3)

Fig. 1
Fig. 1

Experimental measurements from a tissue mimicking phantom. (a) Spectral difference and (b) spectral ratio using the IMMI method. (c) Plot along the dashed line of (a) and (b): black, assumed correct profile; red, spectral difference; blue, spectral ratio. (d) Spectral ratio using the back projection method.

Fig. 2
Fig. 2

Spectral difference and ratio methods from a phantom contained two identical round insertions, as indicated by the open circles on (a). The insertions (A, B) had μ a ( λ 1 ) = 0.42 cm 1 ) and μ a ( λ 2 ) = 0.4 cm 1 and the same μ s = 10 cm 1 , the background had an absorption coefficient of μ a = 0.35 cm 1 for both wavelengths. (a) Reconstructed image at λ 1 , capturing the effects of photon distribution in the phantom, typical of the IMMI reconstructions. (b) Spectral difference. (c) Reconstructed profiles along the white line on (b). (d) Spectral ratio of the two images reconstructed at λ 1 , λ 2 .

Fig. 3
Fig. 3

(a) Optoacoustic image of a mouse with ICG filled tubes at 800 nm . (b) Sketch of the mouse and the implanted tubes. (c) Spectral difference. (d) Spectral ratio. (e) Profiles along the dashed lines of (b) for spectral ratio and spectral difference. (f) Superimposed image of (d) on (a) after application of a threshold at I = 0.5 .

Equations (3)

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H ( λ , r ) μ a ( λ , r ) U ( λ , r ) ,
Δ H ( r ) = H ( λ 1 , r ) H ( λ 2 , r ) U ( λ 1 , r ) [ μ a ( λ 1 , r ) q μ a ( λ 2 , r ) ] .
I μ a ( λ 1 , r ) q μ a ( λ 2 , r ) μ a ( λ 1 , r ) .

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