Abstract

We present an optical trapping system combining individually addressable multiple laser traps with fluorescence spectroscopy. An in-line set of 64 near-IR laser diodes is used to create a line of individually addressable traps inside a microfluidic chip. This system is completed by an excitation/detection line for spectrally resolved fluorescence imaging of trapped particles. Highly parallel trapping in a constant flow (up to a few millimeters per second), fast particle handling rates (up to a few particles per second), and the possibility of recording fluorescence spectra of trapped objects lead to a performing bioanalytical platform, e.g., for highly parallel analysis and sorting.

© 2011 Optical Society of America

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2010 (1)

2007 (1)

G. Boer, R. Johann, J. Rohner, F. Merenda, G. Delacrétaz, Ph. Renaud, and R. P. Salathe, Rev. Sci. Instrum. 78, 116101 (2007).
[CrossRef] [PubMed]

2006 (1)

D. McGloin, Phil. Trans. R. Soc. A 364, 3521 (2006).
[CrossRef] [PubMed]

2005 (4)

E. A. Abbondanzieri, W. J. Greenleaf, J. W. Shaevitz, R. Landick, and S. M. Block, Nature 438, 460 (2005).
[CrossRef] [PubMed]

R. Agarwal, K. Ladavac, Y. Roichman, G. Yu, C. M. Lieber, and D. G. Grier, Opt. Express 13, 8906 (2005).
[CrossRef] [PubMed]

R. W. Applegate Jr., J. Squier, T. Vestad, J. Oakey, and D. W. M. Marr, in Conference on Lasers and Electro-Optics/Quantum Electronics and Laser Science and Photonic Applications Systems Technologies, OSA Technical Digest (CD) (Optical Society of America, 2005), paper CFN2.
[PubMed]

D. S. Kaputa, A. N. Kuzmin, A. V. Kachynski, A. N. Cartwright, and P. N. Prasad, Appl. Opt. 44, 3963 (2005).
[CrossRef] [PubMed]

2004 (1)

D. L. J. Vossen, A. van der Horst, M. Dogterom, and A. Van Blaaderen, Rev. Sci. Instrum. 75, 2960 (2004).
[CrossRef]

2002 (2)

R. L. Eriksen, P. C. Mogensen, and J. Gluckstad, Opt. Lett. 27, 267 (2002).
[CrossRef]

J. E. Curtis, B. A. Koss, and D. G. Grier, Opt. Commun. 207, 169 (2002).
[CrossRef]

2001 (1)

1997 (1)

1994 (1)

1986 (1)

1970 (1)

A. Ashkin, Phys. Rev. Lett. 24, 156 (1970).
[CrossRef]

Abbondanzieri, E. A.

E. A. Abbondanzieri, W. J. Greenleaf, J. W. Shaevitz, R. Landick, and S. M. Block, Nature 438, 460 (2005).
[CrossRef] [PubMed]

Agarwal, R.

Applegate, R. W.

R. W. Applegate Jr., J. Squier, T. Vestad, J. Oakey, and D. W. M. Marr, in Conference on Lasers and Electro-Optics/Quantum Electronics and Laser Science and Photonic Applications Systems Technologies, OSA Technical Digest (CD) (Optical Society of America, 2005), paper CFN2.
[PubMed]

Ashkin, A.

Axner, O.

Berns, M. W.

Bjorkholm, J. E.

Block, S. M.

E. A. Abbondanzieri, W. J. Greenleaf, J. W. Shaevitz, R. Landick, and S. M. Block, Nature 438, 460 (2005).
[CrossRef] [PubMed]

Boer, G.

G. Boer, R. Johann, J. Rohner, F. Merenda, G. Delacrétaz, Ph. Renaud, and R. P. Salathe, Rev. Sci. Instrum. 78, 116101 (2007).
[CrossRef] [PubMed]

Cartwright, A. N.

Chu, S.

Curtis, J. E.

J. E. Curtis, B. A. Koss, and D. G. Grier, Opt. Commun. 207, 169 (2002).
[CrossRef]

Delacrétaz, G.

G. Boer, R. Johann, J. Rohner, F. Merenda, G. Delacrétaz, Ph. Renaud, and R. P. Salathe, Rev. Sci. Instrum. 78, 116101 (2007).
[CrossRef] [PubMed]

Dogterom, M.

D. L. J. Vossen, A. van der Horst, M. Dogterom, and A. Van Blaaderen, Rev. Sci. Instrum. 75, 2960 (2004).
[CrossRef]

Dziedzic, J. M.

Eriksen, R. L.

Fallman, E.

Gluckstad, J.

Greenleaf, W. J.

E. A. Abbondanzieri, W. J. Greenleaf, J. W. Shaevitz, R. Landick, and S. M. Block, Nature 438, 460 (2005).
[CrossRef] [PubMed]

Grier, D. G.

Johann, R.

G. Boer, R. Johann, J. Rohner, F. Merenda, G. Delacrétaz, Ph. Renaud, and R. P. Salathe, Rev. Sci. Instrum. 78, 116101 (2007).
[CrossRef] [PubMed]

Kachynski, A. V.

Kagawa, K.

Kaputa, D. S.

Kong, L.

Koss, B. A.

J. E. Curtis, B. A. Koss, and D. G. Grier, Opt. Commun. 207, 169 (2002).
[CrossRef]

Kuzmin, A. N.

Ladavac, K.

Landick, R.

E. A. Abbondanzieri, W. J. Greenleaf, J. W. Shaevitz, R. Landick, and S. M. Block, Nature 438, 460 (2005).
[CrossRef] [PubMed]

Li, Y.-Q.

Lieber, C. M.

Marr, D. W. M.

R. W. Applegate Jr., J. Squier, T. Vestad, J. Oakey, and D. W. M. Marr, in Conference on Lasers and Electro-Optics/Quantum Electronics and Laser Science and Photonic Applications Systems Technologies, OSA Technical Digest (CD) (Optical Society of America, 2005), paper CFN2.
[PubMed]

McGloin, D.

D. McGloin, Phil. Trans. R. Soc. A 364, 3521 (2006).
[CrossRef] [PubMed]

Merenda, F.

G. Boer, R. Johann, J. Rohner, F. Merenda, G. Delacrétaz, Ph. Renaud, and R. P. Salathe, Rev. Sci. Instrum. 78, 116101 (2007).
[CrossRef] [PubMed]

Mogensen, P. C.

Oakey, J.

R. W. Applegate Jr., J. Squier, T. Vestad, J. Oakey, and D. W. M. Marr, in Conference on Lasers and Electro-Optics/Quantum Electronics and Laser Science and Photonic Applications Systems Technologies, OSA Technical Digest (CD) (Optical Society of America, 2005), paper CFN2.
[PubMed]

Ogura, Y.

Prasad, P. N.

Renaud, Ph.

G. Boer, R. Johann, J. Rohner, F. Merenda, G. Delacrétaz, Ph. Renaud, and R. P. Salathe, Rev. Sci. Instrum. 78, 116101 (2007).
[CrossRef] [PubMed]

Rohner, J.

G. Boer, R. Johann, J. Rohner, F. Merenda, G. Delacrétaz, Ph. Renaud, and R. P. Salathe, Rev. Sci. Instrum. 78, 116101 (2007).
[CrossRef] [PubMed]

Roichman, Y.

Salathe, R. P.

G. Boer, R. Johann, J. Rohner, F. Merenda, G. Delacrétaz, Ph. Renaud, and R. P. Salathe, Rev. Sci. Instrum. 78, 116101 (2007).
[CrossRef] [PubMed]

Setlow, P.

Shaevitz, J. W.

E. A. Abbondanzieri, W. J. Greenleaf, J. W. Shaevitz, R. Landick, and S. M. Block, Nature 438, 460 (2005).
[CrossRef] [PubMed]

Sonek, G. J.

Squier, J.

R. W. Applegate Jr., J. Squier, T. Vestad, J. Oakey, and D. W. M. Marr, in Conference on Lasers and Electro-Optics/Quantum Electronics and Laser Science and Photonic Applications Systems Technologies, OSA Technical Digest (CD) (Optical Society of America, 2005), paper CFN2.
[PubMed]

Tanida, J.

Van Blaaderen, A.

D. L. J. Vossen, A. van der Horst, M. Dogterom, and A. Van Blaaderen, Rev. Sci. Instrum. 75, 2960 (2004).
[CrossRef]

van der Horst, A.

D. L. J. Vossen, A. van der Horst, M. Dogterom, and A. Van Blaaderen, Rev. Sci. Instrum. 75, 2960 (2004).
[CrossRef]

Vestad, T.

R. W. Applegate Jr., J. Squier, T. Vestad, J. Oakey, and D. W. M. Marr, in Conference on Lasers and Electro-Optics/Quantum Electronics and Laser Science and Photonic Applications Systems Technologies, OSA Technical Digest (CD) (Optical Society of America, 2005), paper CFN2.
[PubMed]

Vossen, D. L. J.

D. L. J. Vossen, A. van der Horst, M. Dogterom, and A. Van Blaaderen, Rev. Sci. Instrum. 75, 2960 (2004).
[CrossRef]

Wright, W. H.

Yu, G.

Zhang, P.

Appl. Opt. (4)

Nature (1)

E. A. Abbondanzieri, W. J. Greenleaf, J. W. Shaevitz, R. Landick, and S. M. Block, Nature 438, 460 (2005).
[CrossRef] [PubMed]

Opt. Commun. (1)

J. E. Curtis, B. A. Koss, and D. G. Grier, Opt. Commun. 207, 169 (2002).
[CrossRef]

Opt. Express (1)

Opt. Lett. (3)

Phil. Trans. R. Soc. A (1)

D. McGloin, Phil. Trans. R. Soc. A 364, 3521 (2006).
[CrossRef] [PubMed]

Phys. Rev. Lett. (1)

A. Ashkin, Phys. Rev. Lett. 24, 156 (1970).
[CrossRef]

Rev. Sci. Instrum. (2)

D. L. J. Vossen, A. van der Horst, M. Dogterom, and A. Van Blaaderen, Rev. Sci. Instrum. 75, 2960 (2004).
[CrossRef]

G. Boer, R. Johann, J. Rohner, F. Merenda, G. Delacrétaz, Ph. Renaud, and R. P. Salathe, Rev. Sci. Instrum. 78, 116101 (2007).
[CrossRef] [PubMed]

Other (1)

R. W. Applegate Jr., J. Squier, T. Vestad, J. Oakey, and D. W. M. Marr, in Conference on Lasers and Electro-Optics/Quantum Electronics and Laser Science and Photonic Applications Systems Technologies, OSA Technical Digest (CD) (Optical Society of America, 2005), paper CFN2.
[PubMed]

Supplementary Material (1)

» Media 1: MOV (529 KB)     

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Figures (4)

Fig. 1
Fig. 1

Simplified schematic description of the system. LD, laser diodes; L1-L4, lenses; BS1, BS2, beam splitters; MO, microscope objective. Trapping and fluorescence excitation occur at the focal plane of the MO.

Fig. 2
Fig. 2

Microfluidic system. Inset: Control of flow direction illustration. Filling tanks (A, B, C) allow dynamic reactant handling; syringe pumps (D, E) allow accurate flow control.

Fig. 3
Fig. 3

Steps of the filling traps process.

Fig. 4
Fig. 4

(a) Individual trap handling and (b) simultaneous fluorescence measurement (Media 1).

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