Abstract

We propose an optical configuration in which floating particles in a microfluidic chamber can be characterized by an interference microscopy configuration to obtain quantitative phase-contrast maps. The configuration is simply made by two laser beams from the same laser source. One beam provides the optical forces for driving the particle along appropriate paths, but at same time works as the object illumination beam in the holographic microscope. The second beam plays the role of the reference beam, allowing recording of an interference fringe pattern (i.e., the digital hologram) in an out-of-focus image plane. The system and method are illustrated and experimental results are offered for polymeric particles as well as for in vitro cells with the aim to demonstrate the approach.

© 2011 Optical Society of America

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References

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2011 (8)

C. Song, T.-D. Luong, T. Fook Kong, N.-T. Nguyen, and A. K. Asundi, Opt. Lett. 36, 657 (2011).
[CrossRef] [PubMed]

J. A. Rodrigo, A. M. Caravaca-Aguirre, T. Alieva, G. Cristóbal, and M. L. Calvo, Opt. Express 19, 5232 (2011).
[CrossRef] [PubMed]

M. J. Padgett and R. Di Leonardo, Lab Chip 11, 1196 (2011).
[CrossRef] [PubMed]

S. O. Isikman, W. Bishara, S. Mavandadi, F. W. Yu, S. Feng, R. Lau, and A. Ozcan, Proc. Natl. Acad. Sci. USA 108, 7296 (2011).
[CrossRef] [PubMed]

Z. WangL. Millet, M. Mir, H. Ding, S. Unarunotai, J. Rogers, M. U. Gillette, and G. Popescu, Opt. Express 19, 1016 (2011).
[CrossRef] [PubMed]

S. O. Isikman, W. Bishara, H. Zhu, and A. Ozcan, Appl. Phys. Lett. 98, 161109 (2011).
[CrossRef] [PubMed]

A. El Mallahi and F. Dubois, Opt. Express 19, 6684 (2011).
[CrossRef] [PubMed]

B. Kemper, A. Vollmer, C. E. Rommel, J. Schnekenburger, and G. von Bally, J. Biomed. Opt. 16, 026014 (2011).
[CrossRef] [PubMed]

2010 (9)

2008 (2)

2007 (2)

L. MiccioD. Alfieri, S. Grilli, P. Ferraro, A. Finizio, L. De Petrocellis, and S. D. Nicola, Appl. Phys. Lett. 90, 041104 (2007).
[CrossRef]

Y. Park, G. Popescu, K. Badizadegan, R. R. Dasari, and M. S. Feld, Opt. Lett. 32, 811 (2007).
[CrossRef] [PubMed]

2006 (3)

F. Dubois, C. Yourassowsky, O. Monnom, J. C. Legros, O. Debeir, P. Van Ham, R. Kiss, and C. Decaestecker, J. Biomed. Opt. 11, 054032 (2006).
[CrossRef] [PubMed]

D. Psaltis, S. R. Quake, and C. Yang, Nature 442, 381 (2006).
[CrossRef] [PubMed]

P. Ferraro, D. Alferi, S. De Nicola, L. De Petrocellis, A. Finizio, and G. Pierattini, Opt. Lett. 31, 1405 (2006).
[CrossRef] [PubMed]

2004 (1)

H. Yang, N. Halliwell, and J. Coupland, Meas. Sci. Technol. 15, 694 (2004).
[CrossRef]

2003 (2)

2001 (1)

Alferi, D.

Alfieri, D.

L. MiccioD. Alfieri, S. Grilli, P. Ferraro, A. Finizio, L. De Petrocellis, and S. D. Nicola, Appl. Phys. Lett. 90, 041104 (2007).
[CrossRef]

Alieva, T.

Antkowiak, M.

Ash, W. M.

Asundi, A. K.

Badizadegan, K.

Balduzzi, D.

Bishara, W.

S. O. Isikman, W. Bishara, H. Zhu, and A. Ozcan, Appl. Phys. Lett. 98, 161109 (2011).
[CrossRef] [PubMed]

S. O. Isikman, W. Bishara, S. Mavandadi, F. W. Yu, S. Feng, R. Lau, and A. Ozcan, Proc. Natl. Acad. Sci. USA 108, 7296 (2011).
[CrossRef] [PubMed]

T.-W. Su, S. O. Isikman, W. Bishara, D. Tseng, A. Erlinger, and A. Ozcan, Opt. Express 18, 9690 (2010).
[CrossRef] [PubMed]

W. Bishara, H. Zhu, and A. Ozcan, Opt. Express 18, 27499(2010).
[CrossRef]

Bursac, N.

Calvo, M. L.

Caravaca-Aguirre, A. M.

Charrière, F.

Cheong, F. C.

Cižmár, T.

T. Čižmár, M. Mazilu, and K. Dholakia, Nat. Photonics 4, 388 (2010).
[CrossRef]

Coppola, G.

Coupland, J.

H. Yang, N. Halliwell, and J. Coupland, Meas. Sci. Technol. 15, 694 (2004).
[CrossRef]

Cristóbal, G.

DaneshPanah, M.

M. DaneshPanah, S. Zwick, F. Schaal, M. Warber, B. Javidi, and W. Osten, J. Disp. Technol. 6, 490 (2010).
[CrossRef]

Dasari, R. R.

De Nicola, S.

De Petrocellis, L.

L. MiccioD. Alfieri, S. Grilli, P. Ferraro, A. Finizio, L. De Petrocellis, and S. D. Nicola, Appl. Phys. Lett. 90, 041104 (2007).
[CrossRef]

P. Ferraro, D. Alferi, S. De Nicola, L. De Petrocellis, A. Finizio, and G. Pierattini, Opt. Lett. 31, 1405 (2006).
[CrossRef] [PubMed]

Debeir, O.

F. Dubois, C. Yourassowsky, O. Monnom, J. C. Legros, O. Debeir, P. Van Ham, R. Kiss, and C. Decaestecker, J. Biomed. Opt. 11, 054032 (2006).
[CrossRef] [PubMed]

Decaestecker, C.

F. Dubois, C. Yourassowsky, O. Monnom, J. C. Legros, O. Debeir, P. Van Ham, R. Kiss, and C. Decaestecker, J. Biomed. Opt. 11, 054032 (2006).
[CrossRef] [PubMed]

Depeursinge, C.

Dholakia, K.

Di Caprio, G.

Di Leonardo, R.

M. J. Padgett and R. Di Leonardo, Lab Chip 11, 1196 (2011).
[CrossRef] [PubMed]

Ding, H.

Dubois, F.

A. El Mallahi and F. Dubois, Opt. Express 19, 6684 (2011).
[CrossRef] [PubMed]

F. Dubois, C. Yourassowsky, O. Monnom, J. C. Legros, O. Debeir, P. Van Ham, R. Kiss, and C. Decaestecker, J. Biomed. Opt. 11, 054032 (2006).
[CrossRef] [PubMed]

El Mallahi, A.

Erlinger, A.

Feld, M. S.

Feng, S.

S. O. Isikman, W. Bishara, S. Mavandadi, F. W. Yu, S. Feng, R. Lau, and A. Ozcan, Proc. Natl. Acad. Sci. USA 108, 7296 (2011).
[CrossRef] [PubMed]

Ferraro, P.

Finizio, A.

Fook Kong, T.

Galli, A.

Gillette, M. U.

Gioffré, M.

Grier, D. G.

Grilli, S.

Gunn-Moore, F. J.

Halliwell, N.

H. Yang, N. Halliwell, and J. Coupland, Meas. Sci. Technol. 15, 694 (2004).
[CrossRef]

Hart, S. J.

Isikman, S. O.

S. O. Isikman, W. Bishara, S. Mavandadi, F. W. Yu, S. Feng, R. Lau, and A. Ozcan, Proc. Natl. Acad. Sci. USA 108, 7296 (2011).
[CrossRef] [PubMed]

S. O. Isikman, W. Bishara, H. Zhu, and A. Ozcan, Appl. Phys. Lett. 98, 161109 (2011).
[CrossRef] [PubMed]

T.-W. Su, S. O. Isikman, W. Bishara, D. Tseng, A. Erlinger, and A. Ozcan, Opt. Express 18, 9690 (2010).
[CrossRef] [PubMed]

Javidi, B.

M. DaneshPanah, S. Zwick, F. Schaal, M. Warber, B. Javidi, and W. Osten, J. Disp. Technol. 6, 490 (2010).
[CrossRef]

Kemper, B.

B. Kemper, A. Vollmer, C. E. Rommel, J. Schnekenburger, and G. von Bally, J. Biomed. Opt. 16, 026014 (2011).
[CrossRef] [PubMed]

Kim, M. K.

Kiss, R.

F. Dubois, C. Yourassowsky, O. Monnom, J. C. Legros, O. Debeir, P. Van Ham, R. Kiss, and C. Decaestecker, J. Biomed. Opt. 11, 054032 (2006).
[CrossRef] [PubMed]

Krishnatreya, B. J.

Lall, A. A.

Lau, R.

S. O. Isikman, W. Bishara, S. Mavandadi, F. W. Yu, S. Feng, R. Lau, and A. Ozcan, Proc. Natl. Acad. Sci. USA 108, 7296 (2011).
[CrossRef] [PubMed]

Legros, J. C.

F. Dubois, C. Yourassowsky, O. Monnom, J. C. Legros, O. Debeir, P. Van Ham, R. Kiss, and C. Decaestecker, J. Biomed. Opt. 11, 054032 (2006).
[CrossRef] [PubMed]

Luong, T.-D.

Magistretti, P. J.

Magro, C.

Marquet, P.

Mavandadi, S.

S. O. Isikman, W. Bishara, S. Mavandadi, F. W. Yu, S. Feng, R. Lau, and A. Ozcan, Proc. Natl. Acad. Sci. USA 108, 7296 (2011).
[CrossRef] [PubMed]

Mazilu, M.

T. Čižmár, M. Mazilu, and K. Dholakia, Nat. Photonics 4, 388 (2010).
[CrossRef]

Meucci, R.

Miccio, L.

G. Coppola, G. Di Caprio, M. Gioffré, R. Puglisi, D. Balduzzi, A. Galli, L. Miccio, M. Paturzo, S. Grilli, A. Finizio, and P. Ferraro, Opt. Lett. 35, 3390 (2010).
[CrossRef] [PubMed]

L. MiccioD. Alfieri, S. Grilli, P. Ferraro, A. Finizio, L. De Petrocellis, and S. D. Nicola, Appl. Phys. Lett. 90, 041104 (2007).
[CrossRef]

Millet, L.

Mir, M.

Monnom, O.

F. Dubois, C. Yourassowsky, O. Monnom, J. C. Legros, O. Debeir, P. Van Ham, R. Kiss, and C. Decaestecker, J. Biomed. Opt. 11, 054032 (2006).
[CrossRef] [PubMed]

Nguyen, N.-T.

Nicola, S. D.

L. MiccioD. Alfieri, S. Grilli, P. Ferraro, A. Finizio, L. De Petrocellis, and S. D. Nicola, Appl. Phys. Lett. 90, 041104 (2007).
[CrossRef]

Osten, W.

M. DaneshPanah, S. Zwick, F. Schaal, M. Warber, B. Javidi, and W. Osten, J. Disp. Technol. 6, 490 (2010).
[CrossRef]

Ozcan, A.

S. O. Isikman, W. Bishara, S. Mavandadi, F. W. Yu, S. Feng, R. Lau, and A. Ozcan, Proc. Natl. Acad. Sci. USA 108, 7296 (2011).
[CrossRef] [PubMed]

S. O. Isikman, W. Bishara, H. Zhu, and A. Ozcan, Appl. Phys. Lett. 98, 161109 (2011).
[CrossRef] [PubMed]

W. Bishara, H. Zhu, and A. Ozcan, Opt. Express 18, 27499(2010).
[CrossRef]

T.-W. Su, S. O. Isikman, W. Bishara, D. Tseng, A. Erlinger, and A. Ozcan, Opt. Express 18, 9690 (2010).
[CrossRef] [PubMed]

Padgett, M. J.

M. J. Padgett and R. Di Leonardo, Lab Chip 11, 1196 (2011).
[CrossRef] [PubMed]

Park, Y.

Paturzo, M.

Pierattini, G.

Popescu, G.

Psaltis, D.

D. Psaltis, S. R. Quake, and C. Yang, Nature 442, 381 (2006).
[CrossRef] [PubMed]

Puglisi, R.

Quake, S. R.

D. Psaltis, S. R. Quake, and C. Yang, Nature 442, 381 (2006).
[CrossRef] [PubMed]

Rappaz, B.

Rodrigo, J. A.

Rogers, J.

Rommel, C. E.

B. Kemper, A. Vollmer, C. E. Rommel, J. Schnekenburger, and G. von Bally, J. Biomed. Opt. 16, 026014 (2011).
[CrossRef] [PubMed]

Satterwhite, L. L.

Schaal, F.

M. DaneshPanah, S. Zwick, F. Schaal, M. Warber, B. Javidi, and W. Osten, J. Disp. Technol. 6, 490 (2010).
[CrossRef]

Schnekenburger, J.

B. Kemper, A. Vollmer, C. E. Rommel, J. Schnekenburger, and G. von Bally, J. Biomed. Opt. 16, 026014 (2011).
[CrossRef] [PubMed]

Shaked, N. T.

Song, C.

Su, T.-W.

Terray, A.

Torres-Mapa, M. L.

Tseng, D.

Unarunotai, S.

Van Ham, P.

F. Dubois, C. Yourassowsky, O. Monnom, J. C. Legros, O. Debeir, P. Van Ham, R. Kiss, and C. Decaestecker, J. Biomed. Opt. 11, 054032 (2006).
[CrossRef] [PubMed]

Vollmer, A.

B. Kemper, A. Vollmer, C. E. Rommel, J. Schnekenburger, and G. von Bally, J. Biomed. Opt. 16, 026014 (2011).
[CrossRef] [PubMed]

von Bally, G.

B. Kemper, A. Vollmer, C. E. Rommel, J. Schnekenburger, and G. von Bally, J. Biomed. Opt. 16, 026014 (2011).
[CrossRef] [PubMed]

Wang, Z.

Warber, M.

M. DaneshPanah, S. Zwick, F. Schaal, M. Warber, B. Javidi, and W. Osten, J. Disp. Technol. 6, 490 (2010).
[CrossRef]

Wax, A.

Yang, C.

D. Psaltis, S. R. Quake, and C. Yang, Nature 442, 381 (2006).
[CrossRef] [PubMed]

Yang, H.

H. Yang, N. Halliwell, and J. Coupland, Meas. Sci. Technol. 15, 694 (2004).
[CrossRef]

Yourassowsky, C.

F. Dubois, C. Yourassowsky, O. Monnom, J. C. Legros, O. Debeir, P. Van Ham, R. Kiss, and C. Decaestecker, J. Biomed. Opt. 11, 054032 (2006).
[CrossRef] [PubMed]

Yu, F. W.

S. O. Isikman, W. Bishara, S. Mavandadi, F. W. Yu, S. Feng, R. Lau, and A. Ozcan, Proc. Natl. Acad. Sci. USA 108, 7296 (2011).
[CrossRef] [PubMed]

Zhu, H.

S. O. Isikman, W. Bishara, H. Zhu, and A. Ozcan, Appl. Phys. Lett. 98, 161109 (2011).
[CrossRef] [PubMed]

W. Bishara, H. Zhu, and A. Ozcan, Opt. Express 18, 27499(2010).
[CrossRef]

Zwick, S.

M. DaneshPanah, S. Zwick, F. Schaal, M. Warber, B. Javidi, and W. Osten, J. Disp. Technol. 6, 490 (2010).
[CrossRef]

Appl. Opt. (1)

Appl. Phys. Lett. (2)

L. MiccioD. Alfieri, S. Grilli, P. Ferraro, A. Finizio, L. De Petrocellis, and S. D. Nicola, Appl. Phys. Lett. 90, 041104 (2007).
[CrossRef]

S. O. Isikman, W. Bishara, H. Zhu, and A. Ozcan, Appl. Phys. Lett. 98, 161109 (2011).
[CrossRef] [PubMed]

Biomed. Opt. Express (2)

J. Biomed. Opt. (2)

F. Dubois, C. Yourassowsky, O. Monnom, J. C. Legros, O. Debeir, P. Van Ham, R. Kiss, and C. Decaestecker, J. Biomed. Opt. 11, 054032 (2006).
[CrossRef] [PubMed]

B. Kemper, A. Vollmer, C. E. Rommel, J. Schnekenburger, and G. von Bally, J. Biomed. Opt. 16, 026014 (2011).
[CrossRef] [PubMed]

J. Disp. Technol. (1)

M. DaneshPanah, S. Zwick, F. Schaal, M. Warber, B. Javidi, and W. Osten, J. Disp. Technol. 6, 490 (2010).
[CrossRef]

Lab Chip (1)

M. J. Padgett and R. Di Leonardo, Lab Chip 11, 1196 (2011).
[CrossRef] [PubMed]

Meas. Sci. Technol. (1)

H. Yang, N. Halliwell, and J. Coupland, Meas. Sci. Technol. 15, 694 (2004).
[CrossRef]

Nat. Photonics (1)

T. Čižmár, M. Mazilu, and K. Dholakia, Nat. Photonics 4, 388 (2010).
[CrossRef]

Nature (2)

D. Psaltis, S. R. Quake, and C. Yang, Nature 442, 381 (2006).
[CrossRef] [PubMed]

D. G. Grier, Nature 424, 810 (2003).
[CrossRef] [PubMed]

Opt. Express (9)

Opt. Lett. (5)

Proc. Natl. Acad. Sci. USA (1)

S. O. Isikman, W. Bishara, S. Mavandadi, F. W. Yu, S. Feng, R. Lau, and A. Ozcan, Proc. Natl. Acad. Sci. USA 108, 7296 (2011).
[CrossRef] [PubMed]

Supplementary Material (2)

» Media 1: MOV (3699 KB)     
» Media 2: MOV (944 KB)     

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Figures (5)

Fig. 1
Fig. 1

Optical configuration adopted. The “on-axis” or object illumination beam captures and drives the particle, while the “off-axis” or reference beam generates the hologram (displayed as inset).

Fig. 2
Fig. 2

Sketch of the forces acting on the sample. (a)–(c) Three different positions of a cell while guided in the funnel (Media 1).

Fig. 3
Fig. 3

Images in the focal plane of the first MO. (a) Image of the two separated beams. (b) A particle passes through the on-axis object beam.

Fig. 4
Fig. 4

Reconstructed amplitude of a latex particle.

Fig. 5
Fig. 5

Reconstructed phase maps of (a) a mouse cell and (b) a duplicating mouse cell (Media 2).

Equations (3)

Equations on this page are rendered with MathJax. Learn more.

F scat = n 0 2 c 0 2 π 0 π / 2 I ( r , z ) [ 1 + R cos 2 θ T 2 cos 2 ( θ ϑ ) + R cos 2 θ 1 + R 2 + 2 R cos 2 ϑ ] · ( D p 2 ) 2 sin 2 θ d θ d Θ ,
F grad = n 0 2 c 0 2 π 0 π / 2 I ( r , z ) [ R sin 2 θ T 2 sin 2 ( θ ϑ ) + R sin 2 θ 1 + R 2 + 2 R cos 2 ϑ ] · ( D p 2 ) 2 sin 2 θ cos Θ d θ d Θ ,
I ( r , z ) = 2 P ω 2 ( z ) exp ( 2 r 2 ω 2 ( z ) ) ,

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