A wavelength-coded multifocal microscope incorporating multiplexed and wavelength-coded holographic gratings to generate wavelength-selective multifocal planes is presented. The focal planes are longitudinally spaced on the object plane, and each focal plane is probed by a designated wavelength. The recording of the multiplexed gratings takes place at a single wavelength by utilizing the Bragg degeneracy property; thus the maximum sensitive wavelength of blue is used for recording, but the device is operated at a broad wavelength band of interest, all the way to red . We present the design, implementation, and experimental image data demonstrating this microscope’s ability to obtain biological tissue structures simultaneously at different focal planes using broadband illumination by LEDs.
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