Abstract

The surface plasmon-coupled emission microscope provides high sensitivity for surface imaging. However, it suffers from a distorted donut-shape point-spread function (PSF). Here we report an effective yet simple method to correct for the distortion by introducing a spiral phase plate. This modification converts the donut PSF into one that is single lobed, which is preferable for imaging. The optical performance of the system is characterized and compared with previous publications. This technique provides more than twofold lateral resolution enhancement.

© 2010 Optical Society of America

Full Article  |  PDF Article

References

  • View by:
  • |
  • |
  • |

  1. E. Yeatman and E. Ash, Electron. Lett. 23, 1091 (1987).
    [CrossRef]
  2. B. Rothenhäusler and W. Knoll, Nature 332, 615 (1988).
    [CrossRef]
  3. K.-F. Giebel, C. Bechinger, S. Herminghaus, M. Riedel, P. Leiderer, U. Weiland, and M. Bastmeyer, Biophys. J. 76, 509 (1999).
    [CrossRef] [PubMed]
  4. B. Liedberg, C. Nylander, and I. Lundstrom, Sens. Actuators 4, 299 (1983).
    [CrossRef]
  5. J. Lakowicz, J. Malicka, I. Gryczynski, and Z. Gryczynski, Biochem. Biophys. Res. Commun. 307, 435 (2003).
    [CrossRef] [PubMed]
  6. J. Borejdo, N. Calander, Z. Gryczynski, and I. Gryczynski, Opt. Express 14, 7878 (2006).
    [CrossRef] [PubMed]
  7. T. P. Burghardt, J. E. Charlesworth, M. F. Halstead, J. E. Tarara, and K. Ajtai, Biophys. J. 90, 4662 (2006).
    [CrossRef] [PubMed]
  8. J. Borejdo, Z. Gryczynski, N. Calander, P. Muthu, and I. Gryczynski, Biophys. J. 91, 2626 (2006).
    [CrossRef] [PubMed]
  9. W. T. Tang, E. Chung, Y.-H. Kim, P. T. C. So, and C. J. R. Sheppard, Opt. Express 15, 4634 (2007).
    [CrossRef] [PubMed]
  10. E. Chung, Y.-H. Kim, W. T. Tang, C. J. R. Sheppard, and P. T. C. So, Opt. Lett. 34, 2366 (2009).
    [CrossRef] [PubMed]
  11. C. J. R. Sheppard and K. G. Larkin, Optik 107, 79 (1997).
  12. D. P. Biss, K. S. Youngworth, and T. Brown, Appl. Opt. 45, 470 (2006).
    [CrossRef] [PubMed]
  13. W. T. Tang, Elijah Y. S. Yew, and C. J. R. Sheppard, Opt. Lett. 34, 2147 (2009).
    [CrossRef] [PubMed]

2009 (2)

2007 (1)

2006 (4)

J. Borejdo, N. Calander, Z. Gryczynski, and I. Gryczynski, Opt. Express 14, 7878 (2006).
[CrossRef] [PubMed]

T. P. Burghardt, J. E. Charlesworth, M. F. Halstead, J. E. Tarara, and K. Ajtai, Biophys. J. 90, 4662 (2006).
[CrossRef] [PubMed]

J. Borejdo, Z. Gryczynski, N. Calander, P. Muthu, and I. Gryczynski, Biophys. J. 91, 2626 (2006).
[CrossRef] [PubMed]

D. P. Biss, K. S. Youngworth, and T. Brown, Appl. Opt. 45, 470 (2006).
[CrossRef] [PubMed]

2003 (1)

J. Lakowicz, J. Malicka, I. Gryczynski, and Z. Gryczynski, Biochem. Biophys. Res. Commun. 307, 435 (2003).
[CrossRef] [PubMed]

1999 (1)

K.-F. Giebel, C. Bechinger, S. Herminghaus, M. Riedel, P. Leiderer, U. Weiland, and M. Bastmeyer, Biophys. J. 76, 509 (1999).
[CrossRef] [PubMed]

1997 (1)

C. J. R. Sheppard and K. G. Larkin, Optik 107, 79 (1997).

1988 (1)

B. Rothenhäusler and W. Knoll, Nature 332, 615 (1988).
[CrossRef]

1987 (1)

E. Yeatman and E. Ash, Electron. Lett. 23, 1091 (1987).
[CrossRef]

1983 (1)

B. Liedberg, C. Nylander, and I. Lundstrom, Sens. Actuators 4, 299 (1983).
[CrossRef]

Ajtai, K.

T. P. Burghardt, J. E. Charlesworth, M. F. Halstead, J. E. Tarara, and K. Ajtai, Biophys. J. 90, 4662 (2006).
[CrossRef] [PubMed]

Ash, E.

E. Yeatman and E. Ash, Electron. Lett. 23, 1091 (1987).
[CrossRef]

Bastmeyer, M.

K.-F. Giebel, C. Bechinger, S. Herminghaus, M. Riedel, P. Leiderer, U. Weiland, and M. Bastmeyer, Biophys. J. 76, 509 (1999).
[CrossRef] [PubMed]

Bechinger, C.

K.-F. Giebel, C. Bechinger, S. Herminghaus, M. Riedel, P. Leiderer, U. Weiland, and M. Bastmeyer, Biophys. J. 76, 509 (1999).
[CrossRef] [PubMed]

Biss, D. P.

Borejdo, J.

J. Borejdo, Z. Gryczynski, N. Calander, P. Muthu, and I. Gryczynski, Biophys. J. 91, 2626 (2006).
[CrossRef] [PubMed]

J. Borejdo, N. Calander, Z. Gryczynski, and I. Gryczynski, Opt. Express 14, 7878 (2006).
[CrossRef] [PubMed]

Brown, T.

Burghardt, T. P.

T. P. Burghardt, J. E. Charlesworth, M. F. Halstead, J. E. Tarara, and K. Ajtai, Biophys. J. 90, 4662 (2006).
[CrossRef] [PubMed]

Calander, N.

J. Borejdo, N. Calander, Z. Gryczynski, and I. Gryczynski, Opt. Express 14, 7878 (2006).
[CrossRef] [PubMed]

J. Borejdo, Z. Gryczynski, N. Calander, P. Muthu, and I. Gryczynski, Biophys. J. 91, 2626 (2006).
[CrossRef] [PubMed]

Charlesworth, J. E.

T. P. Burghardt, J. E. Charlesworth, M. F. Halstead, J. E. Tarara, and K. Ajtai, Biophys. J. 90, 4662 (2006).
[CrossRef] [PubMed]

Chung, E.

Giebel, K.-F.

K.-F. Giebel, C. Bechinger, S. Herminghaus, M. Riedel, P. Leiderer, U. Weiland, and M. Bastmeyer, Biophys. J. 76, 509 (1999).
[CrossRef] [PubMed]

Gryczynski, I.

J. Borejdo, N. Calander, Z. Gryczynski, and I. Gryczynski, Opt. Express 14, 7878 (2006).
[CrossRef] [PubMed]

J. Borejdo, Z. Gryczynski, N. Calander, P. Muthu, and I. Gryczynski, Biophys. J. 91, 2626 (2006).
[CrossRef] [PubMed]

J. Lakowicz, J. Malicka, I. Gryczynski, and Z. Gryczynski, Biochem. Biophys. Res. Commun. 307, 435 (2003).
[CrossRef] [PubMed]

Gryczynski, Z.

J. Borejdo, N. Calander, Z. Gryczynski, and I. Gryczynski, Opt. Express 14, 7878 (2006).
[CrossRef] [PubMed]

J. Borejdo, Z. Gryczynski, N. Calander, P. Muthu, and I. Gryczynski, Biophys. J. 91, 2626 (2006).
[CrossRef] [PubMed]

J. Lakowicz, J. Malicka, I. Gryczynski, and Z. Gryczynski, Biochem. Biophys. Res. Commun. 307, 435 (2003).
[CrossRef] [PubMed]

Halstead, M. F.

T. P. Burghardt, J. E. Charlesworth, M. F. Halstead, J. E. Tarara, and K. Ajtai, Biophys. J. 90, 4662 (2006).
[CrossRef] [PubMed]

Herminghaus, S.

K.-F. Giebel, C. Bechinger, S. Herminghaus, M. Riedel, P. Leiderer, U. Weiland, and M. Bastmeyer, Biophys. J. 76, 509 (1999).
[CrossRef] [PubMed]

Kim, Y.-H.

Knoll, W.

B. Rothenhäusler and W. Knoll, Nature 332, 615 (1988).
[CrossRef]

Lakowicz, J.

J. Lakowicz, J. Malicka, I. Gryczynski, and Z. Gryczynski, Biochem. Biophys. Res. Commun. 307, 435 (2003).
[CrossRef] [PubMed]

Larkin, K. G.

C. J. R. Sheppard and K. G. Larkin, Optik 107, 79 (1997).

Leiderer, P.

K.-F. Giebel, C. Bechinger, S. Herminghaus, M. Riedel, P. Leiderer, U. Weiland, and M. Bastmeyer, Biophys. J. 76, 509 (1999).
[CrossRef] [PubMed]

Liedberg, B.

B. Liedberg, C. Nylander, and I. Lundstrom, Sens. Actuators 4, 299 (1983).
[CrossRef]

Lundstrom, I.

B. Liedberg, C. Nylander, and I. Lundstrom, Sens. Actuators 4, 299 (1983).
[CrossRef]

Malicka, J.

J. Lakowicz, J. Malicka, I. Gryczynski, and Z. Gryczynski, Biochem. Biophys. Res. Commun. 307, 435 (2003).
[CrossRef] [PubMed]

Muthu, P.

J. Borejdo, Z. Gryczynski, N. Calander, P. Muthu, and I. Gryczynski, Biophys. J. 91, 2626 (2006).
[CrossRef] [PubMed]

Nylander, C.

B. Liedberg, C. Nylander, and I. Lundstrom, Sens. Actuators 4, 299 (1983).
[CrossRef]

Riedel, M.

K.-F. Giebel, C. Bechinger, S. Herminghaus, M. Riedel, P. Leiderer, U. Weiland, and M. Bastmeyer, Biophys. J. 76, 509 (1999).
[CrossRef] [PubMed]

Rothenhäusler, B.

B. Rothenhäusler and W. Knoll, Nature 332, 615 (1988).
[CrossRef]

Sheppard, C. J. R.

So, P. T. C.

Tang, W. T.

Tarara, J. E.

T. P. Burghardt, J. E. Charlesworth, M. F. Halstead, J. E. Tarara, and K. Ajtai, Biophys. J. 90, 4662 (2006).
[CrossRef] [PubMed]

Weiland, U.

K.-F. Giebel, C. Bechinger, S. Herminghaus, M. Riedel, P. Leiderer, U. Weiland, and M. Bastmeyer, Biophys. J. 76, 509 (1999).
[CrossRef] [PubMed]

Yeatman, E.

E. Yeatman and E. Ash, Electron. Lett. 23, 1091 (1987).
[CrossRef]

Yew, Elijah Y. S.

Youngworth, K. S.

Appl. Opt. (1)

Biochem. Biophys. Res. Commun. (1)

J. Lakowicz, J. Malicka, I. Gryczynski, and Z. Gryczynski, Biochem. Biophys. Res. Commun. 307, 435 (2003).
[CrossRef] [PubMed]

Biophys. J. (3)

T. P. Burghardt, J. E. Charlesworth, M. F. Halstead, J. E. Tarara, and K. Ajtai, Biophys. J. 90, 4662 (2006).
[CrossRef] [PubMed]

J. Borejdo, Z. Gryczynski, N. Calander, P. Muthu, and I. Gryczynski, Biophys. J. 91, 2626 (2006).
[CrossRef] [PubMed]

K.-F. Giebel, C. Bechinger, S. Herminghaus, M. Riedel, P. Leiderer, U. Weiland, and M. Bastmeyer, Biophys. J. 76, 509 (1999).
[CrossRef] [PubMed]

Electron. Lett. (1)

E. Yeatman and E. Ash, Electron. Lett. 23, 1091 (1987).
[CrossRef]

Nature (1)

B. Rothenhäusler and W. Knoll, Nature 332, 615 (1988).
[CrossRef]

Opt. Express (2)

Opt. Lett. (2)

Optik (1)

C. J. R. Sheppard and K. G. Larkin, Optik 107, 79 (1997).

Sens. Actuators (1)

B. Liedberg, C. Nylander, and I. Lundstrom, Sens. Actuators 4, 299 (1983).
[CrossRef]

Cited By

OSA participates in CrossRef's Cited-By Linking service. Citing articles from OSA journals and other participating publishers are listed here.

Alert me when this article is cited.


Figures (4)

Fig. 1
Fig. 1

Setup of modified SPCE microscopy using a high-NA TIRF objective, with an SPP inserted in the infinity-corrected optical path of the detection optics.

Fig. 2
Fig. 2

Emission at the BFP of the objective at time instance t = 0 . (a) Polarization pattern of the electric field without the SPP and (b) changes in polarization when the SPP is inserted. The insets show that the intensity at the BFP remain the same.

Fig. 3
Fig. 3

(a) Comparison of the vectorial OTF of SPCEM without SPP (solid curve) and with SPP (dashed curve) (b) Characteristics of SPP errors: dip ratio I 0 (plus signs) and normalized FWHM (crosses) as a function of misalignment Δ w ; I 0 (circles) and FWHM (diamonds) as a function of wavelength mismatch Δ λ λ .

Fig. 4
Fig. 4

Comparison between simulation and experimental results. Simulated PSF (a) without SPP and (b) with SPP; experimental PSF (c) without SPP and (d) with SPP. Image scale is 2 μ m × 2 μ m .

Equations (2)

Equations on this page are rendered with MathJax. Learn more.

d = d 0 + λ ϕ 2 π ( n 1 ) ,
H OTF ( l ) = FT 2 D [ h IPSF ( r ) ] = i x , y , z P i ( l ) P i * ( l ) ,

Metrics