Abstract

We propose three-dimensional (3D) optical microscopy using axially distributed image sensing. In the proposed method, the micro-objects are optically magnified and their axially distributed images are recorded by moving the image sensor along a common optical axis. The 3D volumetric images are generated from the recorded axial image set using a computational reconstruction algorithm based on ray backprojection. Preliminary experimental results are presented. To the best of our knowledge, this is the first report on 3D optical microscopy using axially distributed sensing.

© 2010 Optical Society of America

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References

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2009 (2)

2006 (5)

F. Charrière, A. Marian, F. Montfort, J. Kuehn, T. Colomb, E. Cuche, P. Marquet, and C. Depeursinge, Opt. Lett. 31, 178 (2006).
[CrossRef] [PubMed]

B. Javidi, I. Moon, and S. Yeom, Opt. Express 14, 12096 (2006).
[CrossRef] [PubMed]

A. Stern and B. Javidi, Proc. IEEE 94, 591 (2006).
[CrossRef]

F. Okano, J. Arai, K. Mitani, and M. Okui, Proc. IEEE 94, 490 (2006).
[CrossRef]

Y. Frauel, T. Naughton, O. Matoba, E. Tahajuerce, and B. Javidi, Proc. IEEE 94, 636 (2006).
[CrossRef]

2005 (3)

2004 (1)

2002 (1)

1999 (1)

1968 (1)

1949 (1)

D. Gabor, Proc. Roy. Soc. A 197, 454 (1949).
[CrossRef]

1908 (1)

G. Lippmann, C. R. Acad. Sci. Paris Ser. IV 146, 446(1908).

Alfieri, D.

Arai, J.

F. Okano, J. Arai, K. Mitani, and M. Okui, Proc. IEEE 94, 490 (2006).
[CrossRef]

Benton, S. A.

S. A. Benton and V. M. Bove, Jr., Holographic Imaging(Wiley, 2008).
[CrossRef]

Bove, V. M.

S. A. Benton and V. M. Bove, Jr., Holographic Imaging(Wiley, 2008).
[CrossRef]

Burckhardt, C. B.

Charrière, F.

Colomb, T.

Coppola, G.

Cuche, E.

DaneshPanah, M.

Depeursinge, C.

Dubois, F.

Ferraro, P.

Finizio, A.

Frauel, Y.

Y. Frauel, T. Naughton, O. Matoba, E. Tahajuerce, and B. Javidi, Proc. IEEE 94, 636 (2006).
[CrossRef]

Gabor, D.

D. Gabor, Proc. Roy. Soc. A 197, 454 (1949).
[CrossRef]

Grilli, S.

Jang, J.-S.

Javidi, B.

Joannes, L.

Kreis, T.

T. Kreis, Handbook of Holographic Interferometry(Wiley, 2005).

Kuehn, J.

Legros, J.-C.

Levoy, M.

M. Levoy, Z. Zhang, and I. McDowall, J. Microsc. 235, 144 (2009).
[CrossRef] [PubMed]

Lippmann, G.

G. Lippmann, C. R. Acad. Sci. Paris Ser. IV 146, 446(1908).

Marian, A.

Marquet, P.

Martinez-Corral, M.

M. Martinez-Corral and G. Saavedra, in Progress in Optics (Elsevier, 2009), Vol. 53, pp. 1-68.
[CrossRef]

Martínez-León, L.

Matoba, O.

Y. Frauel, T. Naughton, O. Matoba, E. Tahajuerce, and B. Javidi, Proc. IEEE 94, 636 (2006).
[CrossRef]

McDowall, I.

M. Levoy, Z. Zhang, and I. McDowall, J. Microsc. 235, 144 (2009).
[CrossRef] [PubMed]

Mitani, K.

F. Okano, J. Arai, K. Mitani, and M. Okui, Proc. IEEE 94, 490 (2006).
[CrossRef]

Montfort, F.

Moon, I.

Naughton, T.

Y. Frauel, T. Naughton, O. Matoba, E. Tahajuerce, and B. Javidi, Proc. IEEE 94, 636 (2006).
[CrossRef]

Nicola, S. D.

Okano, F.

F. Okano, J. Arai, K. Mitani, and M. Okui, Proc. IEEE 94, 490 (2006).
[CrossRef]

Okui, M.

F. Okano, J. Arai, K. Mitani, and M. Okui, Proc. IEEE 94, 490 (2006).
[CrossRef]

Osten, W.

Pawley, J. B.

J. B. Pawley, Handbook of Biological Confocal Microscopy (Springer, 2006).
[CrossRef]

Pedrini, G.

Pierattini, G.

Saavedra, G.

M. Martinez-Corral and G. Saavedra, in Progress in Optics (Elsevier, 2009), Vol. 53, pp. 1-68.
[CrossRef]

Schulein, R.

Sibarita, J. B.

J. B. Sibarita, Adv. Biochem. Eng. Biotech. 95, 201(2005).

Stern, A.

A. Stern and B. Javidi, Proc. IEEE 94, 591 (2006).
[CrossRef]

Striano, V.

Tahajuerce, E.

Y. Frauel, T. Naughton, O. Matoba, E. Tahajuerce, and B. Javidi, Proc. IEEE 94, 636 (2006).
[CrossRef]

Yeom, S.

Zhang, Z.

M. Levoy, Z. Zhang, and I. McDowall, J. Microsc. 235, 144 (2009).
[CrossRef] [PubMed]

Adv. Biochem. Eng. Biotech. (1)

J. B. Sibarita, Adv. Biochem. Eng. Biotech. 95, 201(2005).

Appl. Opt. (2)

C. R. Acad. Sci. Paris Ser. IV (1)

G. Lippmann, C. R. Acad. Sci. Paris Ser. IV 146, 446(1908).

J. Microsc. (1)

M. Levoy, Z. Zhang, and I. McDowall, J. Microsc. 235, 144 (2009).
[CrossRef] [PubMed]

J. Opt. Soc. Am. (1)

Opt. Express (2)

Opt. Lett. (4)

Proc. IEEE (3)

Y. Frauel, T. Naughton, O. Matoba, E. Tahajuerce, and B. Javidi, Proc. IEEE 94, 636 (2006).
[CrossRef]

A. Stern and B. Javidi, Proc. IEEE 94, 591 (2006).
[CrossRef]

F. Okano, J. Arai, K. Mitani, and M. Okui, Proc. IEEE 94, 490 (2006).
[CrossRef]

Proc. Roy. Soc. A (1)

D. Gabor, Proc. Roy. Soc. A 197, 454 (1949).
[CrossRef]

Other (4)

M. Martinez-Corral and G. Saavedra, in Progress in Optics (Elsevier, 2009), Vol. 53, pp. 1-68.
[CrossRef]

S. A. Benton and V. M. Bove, Jr., Holographic Imaging(Wiley, 2008).
[CrossRef]

T. Kreis, Handbook of Holographic Interferometry(Wiley, 2005).

J. B. Pawley, Handbook of Biological Confocal Microscopy (Springer, 2006).
[CrossRef]

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Figures (5)

Fig. 1
Fig. 1

Setup of proposed 3D microscopy using axially distributed image sensing.

Fig. 2
Fig. 2

Pickup process of the axially distributed image sensing.

Fig. 3
Fig. 3

(a) Photograph of an insect used in the experiments. (b) Photograph of the experimental setup for the optical microscope system.

Fig. 4
Fig. 4

Examples of the axially distributed recorded elemental images: (a) first elemental image and (b) thirtieth elemental image.

Fig. 5
Fig. 5

3D computational reconstruction of the insect. Two reconstructed slices of the insect at different longitudinal distances from the sensor: (a) reconstruction at z = 350 mm and (b) at z = 450 mm .

Equations (3)

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M = M 1 M 2 = M 1 ( f l 1 f ) .
δ c z K 2 g r c z K 1 .
R ( x , y , z ) = 1 K k = 0 K 1 E k ( x S k ( z ) / S 0 ( z ) , y S k ( z ) / S 0 ( z ) , z ) = 1 K k = 0 K 1 E k ( z z k Δ z x , z z - k Δ z y , z ) .

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