Abstract

Recently, we have used polarimetry as a method for assessing the linear retardance of infarcted myocardium. While linear retardance reflects tissue anisotropy, experimental geometry has a confounding effect due to dependence of the linear retardance on the orientation of the sample with respect to the probing beam. Here, polarimetry imaging of an 8mm diameter birefringent polystyrene sphere of known anisotropy axis was used to test a dual-projection method by which the anisotropy axis and its true magnitude can be reconstructed, thus eliminating the confounding effect of anisotropy axis orientation. Feasibility is demonstrated in ex-vivo tissue imaging.

© 2010 Optical Society of America

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  1. J. Ramella-Roman, K. Lee, S. Prahl, and S. Jacques, J. Biomed Opt. 9, 1305 (2004).
    [CrossRef] [PubMed]
  2. Z. Tannous, M. Al-Arashi, S. Shah, and A. Yaroslavsky, Lasers Surg. Med. 41, 10 (2009).
    [CrossRef] [PubMed]
  3. P. Bagnaninchi, Y. Yang, M. Bonesi, G. Maffulli, C. Phelan, I. Meglinski, A. El Haj, and N. Naffulli, Phys. Med. Biol. 55, 3777 (2010).
    [CrossRef] [PubMed]
  4. M. van Turnhout, S. Kranenbarg, and J. van Leeuwen, J. Biomed Opt. 14, 054018 (2009).
    [CrossRef] [PubMed]
  5. X. Li and G. Yao, Appl. Opt. 48, 2625 (2009).
    [CrossRef] [PubMed]
  6. N. Ghosh, M. Wood, S. Li, R. Weisel, B. Wilson, R. Li, and I. A. Vitkin, J. Biophoton. 2, 145 (2009).
    [CrossRef]
  7. M. Wood, N. Ghosh, M. A. Wallenburg, S. Li, R. Weisel, B. Wilson, R. Li, and I. A. Vitkin, “Polarization birefringence measurements for characterizing the myocardium, including healthy, infarcted and stem cell treated regenerating cardiac tissues,” J. Biomed Opt. (to be published).
  8. B. Saleh and M. Teich, Fundamentals of Photonics, 2nd ed. (Wiley-Interscience, 2007).
  9. N. Ugryumova, J. Jacobs, M. Bonesi, and S. J. Matcher, Osteoarthr. Cartil. 17, 33 (2009).
    [CrossRef]
  10. M. A. Wallenburg, M. Pop, M. Wood, N. Ghosh, G. A. Wright, and I. A. Vitkin, J. Innov. Opt. Health Sci. 3, 109 (2010).
    [CrossRef]
  11. N. Ghosh, M. Wood, and I. A. Vitkin, J. Biomed Opt. 13, 044036 (2008).
    [CrossRef] [PubMed]

2010 (2)

P. Bagnaninchi, Y. Yang, M. Bonesi, G. Maffulli, C. Phelan, I. Meglinski, A. El Haj, and N. Naffulli, Phys. Med. Biol. 55, 3777 (2010).
[CrossRef] [PubMed]

M. A. Wallenburg, M. Pop, M. Wood, N. Ghosh, G. A. Wright, and I. A. Vitkin, J. Innov. Opt. Health Sci. 3, 109 (2010).
[CrossRef]

2009 (5)

Z. Tannous, M. Al-Arashi, S. Shah, and A. Yaroslavsky, Lasers Surg. Med. 41, 10 (2009).
[CrossRef] [PubMed]

M. van Turnhout, S. Kranenbarg, and J. van Leeuwen, J. Biomed Opt. 14, 054018 (2009).
[CrossRef] [PubMed]

N. Ghosh, M. Wood, S. Li, R. Weisel, B. Wilson, R. Li, and I. A. Vitkin, J. Biophoton. 2, 145 (2009).
[CrossRef]

N. Ugryumova, J. Jacobs, M. Bonesi, and S. J. Matcher, Osteoarthr. Cartil. 17, 33 (2009).
[CrossRef]

X. Li and G. Yao, Appl. Opt. 48, 2625 (2009).
[CrossRef] [PubMed]

2008 (1)

N. Ghosh, M. Wood, and I. A. Vitkin, J. Biomed Opt. 13, 044036 (2008).
[CrossRef] [PubMed]

2004 (1)

J. Ramella-Roman, K. Lee, S. Prahl, and S. Jacques, J. Biomed Opt. 9, 1305 (2004).
[CrossRef] [PubMed]

Al-Arashi, M.

Z. Tannous, M. Al-Arashi, S. Shah, and A. Yaroslavsky, Lasers Surg. Med. 41, 10 (2009).
[CrossRef] [PubMed]

Bagnaninchi, P.

P. Bagnaninchi, Y. Yang, M. Bonesi, G. Maffulli, C. Phelan, I. Meglinski, A. El Haj, and N. Naffulli, Phys. Med. Biol. 55, 3777 (2010).
[CrossRef] [PubMed]

Bonesi, M.

P. Bagnaninchi, Y. Yang, M. Bonesi, G. Maffulli, C. Phelan, I. Meglinski, A. El Haj, and N. Naffulli, Phys. Med. Biol. 55, 3777 (2010).
[CrossRef] [PubMed]

N. Ugryumova, J. Jacobs, M. Bonesi, and S. J. Matcher, Osteoarthr. Cartil. 17, 33 (2009).
[CrossRef]

El Haj, A.

P. Bagnaninchi, Y. Yang, M. Bonesi, G. Maffulli, C. Phelan, I. Meglinski, A. El Haj, and N. Naffulli, Phys. Med. Biol. 55, 3777 (2010).
[CrossRef] [PubMed]

Ghosh, N.

M. A. Wallenburg, M. Pop, M. Wood, N. Ghosh, G. A. Wright, and I. A. Vitkin, J. Innov. Opt. Health Sci. 3, 109 (2010).
[CrossRef]

N. Ghosh, M. Wood, S. Li, R. Weisel, B. Wilson, R. Li, and I. A. Vitkin, J. Biophoton. 2, 145 (2009).
[CrossRef]

N. Ghosh, M. Wood, and I. A. Vitkin, J. Biomed Opt. 13, 044036 (2008).
[CrossRef] [PubMed]

M. Wood, N. Ghosh, M. A. Wallenburg, S. Li, R. Weisel, B. Wilson, R. Li, and I. A. Vitkin, “Polarization birefringence measurements for characterizing the myocardium, including healthy, infarcted and stem cell treated regenerating cardiac tissues,” J. Biomed Opt. (to be published).

Jacobs, J.

N. Ugryumova, J. Jacobs, M. Bonesi, and S. J. Matcher, Osteoarthr. Cartil. 17, 33 (2009).
[CrossRef]

Jacques, S.

J. Ramella-Roman, K. Lee, S. Prahl, and S. Jacques, J. Biomed Opt. 9, 1305 (2004).
[CrossRef] [PubMed]

Kranenbarg, S.

M. van Turnhout, S. Kranenbarg, and J. van Leeuwen, J. Biomed Opt. 14, 054018 (2009).
[CrossRef] [PubMed]

Lee, K.

J. Ramella-Roman, K. Lee, S. Prahl, and S. Jacques, J. Biomed Opt. 9, 1305 (2004).
[CrossRef] [PubMed]

Li, R.

N. Ghosh, M. Wood, S. Li, R. Weisel, B. Wilson, R. Li, and I. A. Vitkin, J. Biophoton. 2, 145 (2009).
[CrossRef]

M. Wood, N. Ghosh, M. A. Wallenburg, S. Li, R. Weisel, B. Wilson, R. Li, and I. A. Vitkin, “Polarization birefringence measurements for characterizing the myocardium, including healthy, infarcted and stem cell treated regenerating cardiac tissues,” J. Biomed Opt. (to be published).

Li, S.

N. Ghosh, M. Wood, S. Li, R. Weisel, B. Wilson, R. Li, and I. A. Vitkin, J. Biophoton. 2, 145 (2009).
[CrossRef]

M. Wood, N. Ghosh, M. A. Wallenburg, S. Li, R. Weisel, B. Wilson, R. Li, and I. A. Vitkin, “Polarization birefringence measurements for characterizing the myocardium, including healthy, infarcted and stem cell treated regenerating cardiac tissues,” J. Biomed Opt. (to be published).

Li, X.

Maffulli, G.

P. Bagnaninchi, Y. Yang, M. Bonesi, G. Maffulli, C. Phelan, I. Meglinski, A. El Haj, and N. Naffulli, Phys. Med. Biol. 55, 3777 (2010).
[CrossRef] [PubMed]

Matcher, S. J.

N. Ugryumova, J. Jacobs, M. Bonesi, and S. J. Matcher, Osteoarthr. Cartil. 17, 33 (2009).
[CrossRef]

Meglinski, I.

P. Bagnaninchi, Y. Yang, M. Bonesi, G. Maffulli, C. Phelan, I. Meglinski, A. El Haj, and N. Naffulli, Phys. Med. Biol. 55, 3777 (2010).
[CrossRef] [PubMed]

Naffulli, N.

P. Bagnaninchi, Y. Yang, M. Bonesi, G. Maffulli, C. Phelan, I. Meglinski, A. El Haj, and N. Naffulli, Phys. Med. Biol. 55, 3777 (2010).
[CrossRef] [PubMed]

Phelan, C.

P. Bagnaninchi, Y. Yang, M. Bonesi, G. Maffulli, C. Phelan, I. Meglinski, A. El Haj, and N. Naffulli, Phys. Med. Biol. 55, 3777 (2010).
[CrossRef] [PubMed]

Pop, M.

M. A. Wallenburg, M. Pop, M. Wood, N. Ghosh, G. A. Wright, and I. A. Vitkin, J. Innov. Opt. Health Sci. 3, 109 (2010).
[CrossRef]

Prahl, S.

J. Ramella-Roman, K. Lee, S. Prahl, and S. Jacques, J. Biomed Opt. 9, 1305 (2004).
[CrossRef] [PubMed]

Ramella-Roman, J.

J. Ramella-Roman, K. Lee, S. Prahl, and S. Jacques, J. Biomed Opt. 9, 1305 (2004).
[CrossRef] [PubMed]

Saleh, B.

B. Saleh and M. Teich, Fundamentals of Photonics, 2nd ed. (Wiley-Interscience, 2007).

Shah, S.

Z. Tannous, M. Al-Arashi, S. Shah, and A. Yaroslavsky, Lasers Surg. Med. 41, 10 (2009).
[CrossRef] [PubMed]

Tannous, Z.

Z. Tannous, M. Al-Arashi, S. Shah, and A. Yaroslavsky, Lasers Surg. Med. 41, 10 (2009).
[CrossRef] [PubMed]

Teich, M.

B. Saleh and M. Teich, Fundamentals of Photonics, 2nd ed. (Wiley-Interscience, 2007).

Ugryumova, N.

N. Ugryumova, J. Jacobs, M. Bonesi, and S. J. Matcher, Osteoarthr. Cartil. 17, 33 (2009).
[CrossRef]

van Leeuwen, J.

M. van Turnhout, S. Kranenbarg, and J. van Leeuwen, J. Biomed Opt. 14, 054018 (2009).
[CrossRef] [PubMed]

van Turnhout, M.

M. van Turnhout, S. Kranenbarg, and J. van Leeuwen, J. Biomed Opt. 14, 054018 (2009).
[CrossRef] [PubMed]

Vitkin, I. A.

M. A. Wallenburg, M. Pop, M. Wood, N. Ghosh, G. A. Wright, and I. A. Vitkin, J. Innov. Opt. Health Sci. 3, 109 (2010).
[CrossRef]

N. Ghosh, M. Wood, S. Li, R. Weisel, B. Wilson, R. Li, and I. A. Vitkin, J. Biophoton. 2, 145 (2009).
[CrossRef]

N. Ghosh, M. Wood, and I. A. Vitkin, J. Biomed Opt. 13, 044036 (2008).
[CrossRef] [PubMed]

M. Wood, N. Ghosh, M. A. Wallenburg, S. Li, R. Weisel, B. Wilson, R. Li, and I. A. Vitkin, “Polarization birefringence measurements for characterizing the myocardium, including healthy, infarcted and stem cell treated regenerating cardiac tissues,” J. Biomed Opt. (to be published).

Wallenburg, M. A.

M. A. Wallenburg, M. Pop, M. Wood, N. Ghosh, G. A. Wright, and I. A. Vitkin, J. Innov. Opt. Health Sci. 3, 109 (2010).
[CrossRef]

M. Wood, N. Ghosh, M. A. Wallenburg, S. Li, R. Weisel, B. Wilson, R. Li, and I. A. Vitkin, “Polarization birefringence measurements for characterizing the myocardium, including healthy, infarcted and stem cell treated regenerating cardiac tissues,” J. Biomed Opt. (to be published).

Weisel, R.

N. Ghosh, M. Wood, S. Li, R. Weisel, B. Wilson, R. Li, and I. A. Vitkin, J. Biophoton. 2, 145 (2009).
[CrossRef]

M. Wood, N. Ghosh, M. A. Wallenburg, S. Li, R. Weisel, B. Wilson, R. Li, and I. A. Vitkin, “Polarization birefringence measurements for characterizing the myocardium, including healthy, infarcted and stem cell treated regenerating cardiac tissues,” J. Biomed Opt. (to be published).

Wilson, B.

N. Ghosh, M. Wood, S. Li, R. Weisel, B. Wilson, R. Li, and I. A. Vitkin, J. Biophoton. 2, 145 (2009).
[CrossRef]

M. Wood, N. Ghosh, M. A. Wallenburg, S. Li, R. Weisel, B. Wilson, R. Li, and I. A. Vitkin, “Polarization birefringence measurements for characterizing the myocardium, including healthy, infarcted and stem cell treated regenerating cardiac tissues,” J. Biomed Opt. (to be published).

Wood, M.

M. A. Wallenburg, M. Pop, M. Wood, N. Ghosh, G. A. Wright, and I. A. Vitkin, J. Innov. Opt. Health Sci. 3, 109 (2010).
[CrossRef]

N. Ghosh, M. Wood, S. Li, R. Weisel, B. Wilson, R. Li, and I. A. Vitkin, J. Biophoton. 2, 145 (2009).
[CrossRef]

N. Ghosh, M. Wood, and I. A. Vitkin, J. Biomed Opt. 13, 044036 (2008).
[CrossRef] [PubMed]

M. Wood, N. Ghosh, M. A. Wallenburg, S. Li, R. Weisel, B. Wilson, R. Li, and I. A. Vitkin, “Polarization birefringence measurements for characterizing the myocardium, including healthy, infarcted and stem cell treated regenerating cardiac tissues,” J. Biomed Opt. (to be published).

Wright, G. A.

M. A. Wallenburg, M. Pop, M. Wood, N. Ghosh, G. A. Wright, and I. A. Vitkin, J. Innov. Opt. Health Sci. 3, 109 (2010).
[CrossRef]

Yang, Y.

P. Bagnaninchi, Y. Yang, M. Bonesi, G. Maffulli, C. Phelan, I. Meglinski, A. El Haj, and N. Naffulli, Phys. Med. Biol. 55, 3777 (2010).
[CrossRef] [PubMed]

Yao, G.

Yaroslavsky, A.

Z. Tannous, M. Al-Arashi, S. Shah, and A. Yaroslavsky, Lasers Surg. Med. 41, 10 (2009).
[CrossRef] [PubMed]

Appl. Opt. (1)

J. Biomed Opt. (3)

N. Ghosh, M. Wood, and I. A. Vitkin, J. Biomed Opt. 13, 044036 (2008).
[CrossRef] [PubMed]

M. van Turnhout, S. Kranenbarg, and J. van Leeuwen, J. Biomed Opt. 14, 054018 (2009).
[CrossRef] [PubMed]

J. Ramella-Roman, K. Lee, S. Prahl, and S. Jacques, J. Biomed Opt. 9, 1305 (2004).
[CrossRef] [PubMed]

J. Biophoton. (1)

N. Ghosh, M. Wood, S. Li, R. Weisel, B. Wilson, R. Li, and I. A. Vitkin, J. Biophoton. 2, 145 (2009).
[CrossRef]

J. Innov. Opt. Health Sci. (1)

M. A. Wallenburg, M. Pop, M. Wood, N. Ghosh, G. A. Wright, and I. A. Vitkin, J. Innov. Opt. Health Sci. 3, 109 (2010).
[CrossRef]

Lasers Surg. Med. (1)

Z. Tannous, M. Al-Arashi, S. Shah, and A. Yaroslavsky, Lasers Surg. Med. 41, 10 (2009).
[CrossRef] [PubMed]

Osteoarthr. Cartil. (1)

N. Ugryumova, J. Jacobs, M. Bonesi, and S. J. Matcher, Osteoarthr. Cartil. 17, 33 (2009).
[CrossRef]

Phys. Med. Biol. (1)

P. Bagnaninchi, Y. Yang, M. Bonesi, G. Maffulli, C. Phelan, I. Meglinski, A. El Haj, and N. Naffulli, Phys. Med. Biol. 55, 3777 (2010).
[CrossRef] [PubMed]

Other (2)

M. Wood, N. Ghosh, M. A. Wallenburg, S. Li, R. Weisel, B. Wilson, R. Li, and I. A. Vitkin, “Polarization birefringence measurements for characterizing the myocardium, including healthy, infarcted and stem cell treated regenerating cardiac tissues,” J. Biomed Opt. (to be published).

B. Saleh and M. Teich, Fundamentals of Photonics, 2nd ed. (Wiley-Interscience, 2007).

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Figures (2)

Fig. 1
Fig. 1

(a) Relative geometry of the sphere anisotropy axis (dark arrow) and the probing beam. The elevation angle φ is the angle between the anisotropy axis and the imaging plane ( x y plane); the azimuthal angle θ is the orientation of the projection of the anisotropy axis on the imaging plane. Only the outgoing beam is shown for clarity. (b) Schematic of the polarimetry imaging system: imaging is done with two different positions of the sphere, separated by a rotation of α about the vertical (y) axis. P, polarizer; L i , lenses; QWP i , quarter-wave plate; A, analyzer. The incident beam is either on axis or off axis by χ (shown here). (c) Apparent and reconstructed linear retardance for different elevation angles φ, and theoretical apparent linear retardance [Eq. (2)] for a sphere with real linear re tardance δ = 41.4 ° . Hollow markers, on axis ( χ = 0 ° ); solid markers, off axis ( χ = 8 ° ).

Fig. 2
Fig. 2

Apparent linear retardance δ app (°) of a pig septum sample at (a) χ = 0 ° and (b) χ = 22 ° . (c) Elevation angle φ. (d) Reconstructed linear retardance δ . Field of view for all: 17 mm × 10 mm .

Equations (3)

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1 n 2 = cos 2 φ n e 2 + sin 2 φ n o 2 ,
δ app = 2 π λ · d · Δ n · [ ( n o n e ) 2 cos 2 φ + sin 2 φ ] 1 / 2 1 n e n o 1 , δ app = 2 π λ · d · Δ n · F ( n e n o , φ ) ,
δ A = δ · F ( φ A ) = δ · F ( tan 1 ( u z u x 2 + u y 2 ) ) ; θ A = tan 1 ( u y u x ) δ B = δ · F ( φ B ) = δ · F ( tan 1 ( u z u x 2 + u y 2 ) ) ; θ B = tan 1 ( u y u x ) .

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