Fluorescence lifetime imaging microscopy (FLIM) offers a noninvasive approach for characterizing the biochemical composition of biological tissue. In recent years, there has been an increasing interest in the application of multispectral FLIM for medical diagnosis. Central to the clinical translation of FLIM technology is the development of robust, fast, and cost-effective FLIM instrumentation suitable for in vivo tissue imaging. Unfortunately, the predominant multispectral FLIM approaches suffer from limitations that impede the development of high-speed instruments for in vivo applications. We present a cost-effective scanning multispectral FLIM implementation capable of achieving pixel rates on the order of tens of kilohertz, which will facilitate the evaluation of FLIM for in vivo applications.
© 2010 Optical Society of AmericaFull Article | PDF Article
Yicong Wu, Wei Zheng, and Jianan Y. Qu
Opt. Lett. 31(21) 3122-3124 (2006)
Riccardo Cicchi, Alfonso Crisci, Alessandro Cosci, Gabriella Nesi, Dimitrios Kapsokalyvas, Saverio Giancane, Marco Carini, and Francesco S. Pavone
Opt. Express 18(4) 3840-3849 (2010)
Jesung Park, Javier A. Jo, Sebina Shrestha, Paritosh Pande, Qiujie Wan, and Brian E. Applegate
Biomed. Opt. Express 1(1) 186-200 (2010)