Abstract

We have developed a new microscopy design that can achieve wide field-of-view (FOV) imaging and yet possesses resolution that is comparable to a conventional microscope. In our design, the sample is illuminated by a holographically projected light-spot grid. We acquire images by translating the sample across the grid and detecting the transmissions. We have built a prototype system with an FOV of 6mm×5mm and acquisition time of 2.5s. The resolution is fundamentally limited by the spot size—our demonstrated average FWHM spot diameter was 0.74μm. We demonstrate the prototype by imaging a U.S. Air Force target and a lily anther. This technology is scalable and represents a cost-effective way to implement wide FOV microscopy systems.

© 2010 Optical Society of America

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2009

S. Seo, T. W. Su, D. K. Tseng, A. Erlinger, and A. Ozcan, Lab Chip 9, 777 (2009).
[CrossRef] [PubMed]

F. Kalkum, S. Broch, T. Brands, and K. Buse, Appl. Phys. B 95, 637 (2009).
[CrossRef]

2008

X. Q. Cui, L. M. Lee, X. Heng, W. W. Zhong, P. W. Sternberg, D. Psaltis, and C. H. Yang, Proc. Natl. Acad. Sci. USA 105, 10670 (2008).
[CrossRef] [PubMed]

2007

M. Oheim, Br. J. Pharmacol. 152, 1 (2007).
[CrossRef] [PubMed]

2006

J. Ho, A. V. Parwani, D. M. Jukic, Y. Yagi, L. Anthony, and J. R. Gilbertson, Hum. Pathol. 37, 322 (2006).
[CrossRef] [PubMed]

2004

C. H. Sow, A. A. Bettiol, Y. Y. G. Lee, F. C. Cheong, C. T. Lim, and F. Watt, Appl. Phys. B 78, 705 (2004).
[CrossRef]

2001

W. B. Xu, M. H. Jericho, I. A. Meinertzhagen, and H. J. Kreuzer, Proc. Natl. Acad. Sci. USA 98, 11301 (2001).
[CrossRef] [PubMed]

1999

1988

Anthony, L.

J. Ho, A. V. Parwani, D. M. Jukic, Y. Yagi, L. Anthony, and J. R. Gilbertson, Hum. Pathol. 37, 322 (2006).
[CrossRef] [PubMed]

Bettiol, A. A.

C. H. Sow, A. A. Bettiol, Y. Y. G. Lee, F. C. Cheong, C. T. Lim, and F. Watt, Appl. Phys. B 78, 705 (2004).
[CrossRef]

Brands, T.

F. Kalkum, S. Broch, T. Brands, and K. Buse, Appl. Phys. B 95, 637 (2009).
[CrossRef]

Broch, S.

F. Kalkum, S. Broch, T. Brands, and K. Buse, Appl. Phys. B 95, 637 (2009).
[CrossRef]

Buse, K.

F. Kalkum, S. Broch, T. Brands, and K. Buse, Appl. Phys. B 95, 637 (2009).
[CrossRef]

Cheong, F. C.

C. H. Sow, A. A. Bettiol, Y. Y. G. Lee, F. C. Cheong, C. T. Lim, and F. Watt, Appl. Phys. B 78, 705 (2004).
[CrossRef]

Cui, X. Q.

X. Q. Cui, L. M. Lee, X. Heng, W. W. Zhong, P. W. Sternberg, D. Psaltis, and C. H. Yang, Proc. Natl. Acad. Sci. USA 105, 10670 (2008).
[CrossRef] [PubMed]

Durnin, J.

Eberly, J. H.

Erlinger, A.

S. Seo, T. W. Su, D. K. Tseng, A. Erlinger, and A. Ozcan, Lab Chip 9, 777 (2009).
[CrossRef] [PubMed]

Gilbertson, J. R.

J. Ho, A. V. Parwani, D. M. Jukic, Y. Yagi, L. Anthony, and J. R. Gilbertson, Hum. Pathol. 37, 322 (2006).
[CrossRef] [PubMed]

Heng, X.

X. Q. Cui, L. M. Lee, X. Heng, W. W. Zhong, P. W. Sternberg, D. Psaltis, and C. H. Yang, Proc. Natl. Acad. Sci. USA 105, 10670 (2008).
[CrossRef] [PubMed]

Ho, J.

J. Ho, A. V. Parwani, D. M. Jukic, Y. Yagi, L. Anthony, and J. R. Gilbertson, Hum. Pathol. 37, 322 (2006).
[CrossRef] [PubMed]

Jericho, M. H.

W. B. Xu, M. H. Jericho, I. A. Meinertzhagen, and H. J. Kreuzer, Proc. Natl. Acad. Sci. USA 98, 11301 (2001).
[CrossRef] [PubMed]

Jukic, D. M.

J. Ho, A. V. Parwani, D. M. Jukic, Y. Yagi, L. Anthony, and J. R. Gilbertson, Hum. Pathol. 37, 322 (2006).
[CrossRef] [PubMed]

Kalkum, F.

F. Kalkum, S. Broch, T. Brands, and K. Buse, Appl. Phys. B 95, 637 (2009).
[CrossRef]

Kreuzer, H. J.

W. B. Xu, M. H. Jericho, I. A. Meinertzhagen, and H. J. Kreuzer, Proc. Natl. Acad. Sci. USA 98, 11301 (2001).
[CrossRef] [PubMed]

Lee, L. M.

X. Q. Cui, L. M. Lee, X. Heng, W. W. Zhong, P. W. Sternberg, D. Psaltis, and C. H. Yang, Proc. Natl. Acad. Sci. USA 105, 10670 (2008).
[CrossRef] [PubMed]

Lee, Y. Y. G.

C. H. Sow, A. A. Bettiol, Y. Y. G. Lee, F. C. Cheong, C. T. Lim, and F. Watt, Appl. Phys. B 78, 705 (2004).
[CrossRef]

Lim, C. T.

C. H. Sow, A. A. Bettiol, Y. Y. G. Lee, F. C. Cheong, C. T. Lim, and F. Watt, Appl. Phys. B 78, 705 (2004).
[CrossRef]

Liu, W. H.

Meinertzhagen, I. A.

W. B. Xu, M. H. Jericho, I. A. Meinertzhagen, and H. J. Kreuzer, Proc. Natl. Acad. Sci. USA 98, 11301 (2001).
[CrossRef] [PubMed]

Miceli, J. J.

Oheim, M.

M. Oheim, Br. J. Pharmacol. 152, 1 (2007).
[CrossRef] [PubMed]

Ozcan, A.

S. Seo, T. W. Su, D. K. Tseng, A. Erlinger, and A. Ozcan, Lab Chip 9, 777 (2009).
[CrossRef] [PubMed]

Parwani, A. V.

J. Ho, A. V. Parwani, D. M. Jukic, Y. Yagi, L. Anthony, and J. R. Gilbertson, Hum. Pathol. 37, 322 (2006).
[CrossRef] [PubMed]

Psaltis, D.

X. Q. Cui, L. M. Lee, X. Heng, W. W. Zhong, P. W. Sternberg, D. Psaltis, and C. H. Yang, Proc. Natl. Acad. Sci. USA 105, 10670 (2008).
[CrossRef] [PubMed]

W. H. Liu and D. Psaltis, Opt. Lett. 24, 1340 (1999).
[CrossRef]

Seo, S.

S. Seo, T. W. Su, D. K. Tseng, A. Erlinger, and A. Ozcan, Lab Chip 9, 777 (2009).
[CrossRef] [PubMed]

Sow, C. H.

C. H. Sow, A. A. Bettiol, Y. Y. G. Lee, F. C. Cheong, C. T. Lim, and F. Watt, Appl. Phys. B 78, 705 (2004).
[CrossRef]

Sternberg, P. W.

X. Q. Cui, L. M. Lee, X. Heng, W. W. Zhong, P. W. Sternberg, D. Psaltis, and C. H. Yang, Proc. Natl. Acad. Sci. USA 105, 10670 (2008).
[CrossRef] [PubMed]

Su, T. W.

S. Seo, T. W. Su, D. K. Tseng, A. Erlinger, and A. Ozcan, Lab Chip 9, 777 (2009).
[CrossRef] [PubMed]

Tseng, D. K.

S. Seo, T. W. Su, D. K. Tseng, A. Erlinger, and A. Ozcan, Lab Chip 9, 777 (2009).
[CrossRef] [PubMed]

Watt, F.

C. H. Sow, A. A. Bettiol, Y. Y. G. Lee, F. C. Cheong, C. T. Lim, and F. Watt, Appl. Phys. B 78, 705 (2004).
[CrossRef]

Xu, W. B.

W. B. Xu, M. H. Jericho, I. A. Meinertzhagen, and H. J. Kreuzer, Proc. Natl. Acad. Sci. USA 98, 11301 (2001).
[CrossRef] [PubMed]

Yagi, Y.

J. Ho, A. V. Parwani, D. M. Jukic, Y. Yagi, L. Anthony, and J. R. Gilbertson, Hum. Pathol. 37, 322 (2006).
[CrossRef] [PubMed]

Yang, C. H.

X. Q. Cui, L. M. Lee, X. Heng, W. W. Zhong, P. W. Sternberg, D. Psaltis, and C. H. Yang, Proc. Natl. Acad. Sci. USA 105, 10670 (2008).
[CrossRef] [PubMed]

Zhong, W. W.

X. Q. Cui, L. M. Lee, X. Heng, W. W. Zhong, P. W. Sternberg, D. Psaltis, and C. H. Yang, Proc. Natl. Acad. Sci. USA 105, 10670 (2008).
[CrossRef] [PubMed]

Appl. Phys. B

F. Kalkum, S. Broch, T. Brands, and K. Buse, Appl. Phys. B 95, 637 (2009).
[CrossRef]

C. H. Sow, A. A. Bettiol, Y. Y. G. Lee, F. C. Cheong, C. T. Lim, and F. Watt, Appl. Phys. B 78, 705 (2004).
[CrossRef]

Br. J. Pharmacol.

M. Oheim, Br. J. Pharmacol. 152, 1 (2007).
[CrossRef] [PubMed]

Hum. Pathol.

J. Ho, A. V. Parwani, D. M. Jukic, Y. Yagi, L. Anthony, and J. R. Gilbertson, Hum. Pathol. 37, 322 (2006).
[CrossRef] [PubMed]

Lab Chip

S. Seo, T. W. Su, D. K. Tseng, A. Erlinger, and A. Ozcan, Lab Chip 9, 777 (2009).
[CrossRef] [PubMed]

Opt. Lett.

Proc. Natl. Acad. Sci. USA

X. Q. Cui, L. M. Lee, X. Heng, W. W. Zhong, P. W. Sternberg, D. Psaltis, and C. H. Yang, Proc. Natl. Acad. Sci. USA 105, 10670 (2008).
[CrossRef] [PubMed]

W. B. Xu, M. H. Jericho, I. A. Meinertzhagen, and H. J. Kreuzer, Proc. Natl. Acad. Sci. USA 98, 11301 (2001).
[CrossRef] [PubMed]

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Figures (3)

Fig. 1
Fig. 1

(a) System setup of the wide FOV microscope system and (b) scanning mechanism employed for imaging.

Fig. 2
Fig. 2

(a) In-line holographic recording scheme for making the hologram, (b) generation of focus grid illumination by holographic reconstruction using the hologram, (c) schematic of the focus grid, and (d)–(f) observation of focus spots from different regions of the focus grid, as indicated in (c), under a microscope using a 60 × objective.

Fig. 3
Fig. 3

(a) Wide FOV image of a U.S. Air Force target, with an effective FOV of 6 mm × 5 mm , as indicated in the large dashed rectangle, (b) expanded view of the smallest feature (groups 6 and 7) of the target, as indicated in (a), (c) wide FOV image of a lily anther, with an effective FOV of 6 mm × 5 mm , as indicated in the large dashed rectangle, and (d) expanded view of the small rectangle area as indicated in (c). S.D., scanning direction.

Equations (1)

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δ x = d sin ( θ ) , δ y = v / F ,

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