Abstract

Recently developed optical-resolution photoacoustic microscopy (OR-PAM), which is based on the detection of optical absorption contrast, is complementary to other optical microscopy modalities such as optical confocal microscopy, optical coherence tomography, and multiphoton microscopy. A hybrid optical–mechanical scanning configuration increases the imaging speed of OR-PAM significantly, facilitating many demanding biomedical applications. With a high-pulse-repetition-rate laser, the hybrid-scanning OR-PAM can acquire one-dimensional depth-resolved images (A-lines) at 5kHz and two-dimensional B-scan images containing 800 A-lines at 6.25Hz. We demonstrated in vivo in a mouse three-dimensional imaging of the iris vasculature in 128s for an 800×800×200 data set and of the ear vasculature in 256s for an 800×1600×200 data set.

© 2010 Optical Society of America

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References

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2010 (1)

G. Ku, K. Maslov, L. Li, and L. V. Wang, J. Biomed. Opt. 15, 09322SSR (2010).
[CrossRef]

2009 (2)

2008 (3)

B. Rao, L. Yu, H. K. Chiang, L. C. Zacharias, R. M. Kurtz, B. D. Kuppermann, and Z. Chen, J. Biomed. Opt. 13, 040505 (2008).
[CrossRef] [PubMed]

L. V. Wang, Med. Phys. 35, 5758 (2008).
[CrossRef]

K. Maslov, H. F. Zhang, S. Hu, and L. V. Wang, Opt. Lett. 33, 929 (2008).
[CrossRef] [PubMed]

2005 (1)

2003 (1)

1999 (1)

L. V. Wang, X. Zhao, H. Sun, and G. Ku, Rev. Sci. Instrum. 70, 3744 (1999).
[CrossRef]

Chen, Z.

B. Rao, L. Yu, H. K. Chiang, L. C. Zacharias, R. M. Kurtz, B. D. Kuppermann, and Z. Chen, J. Biomed. Opt. 13, 040505 (2008).
[CrossRef] [PubMed]

Chiang, H. K.

B. Rao, L. Yu, H. K. Chiang, L. C. Zacharias, R. M. Kurtz, B. D. Kuppermann, and Z. Chen, J. Biomed. Opt. 13, 040505 (2008).
[CrossRef] [PubMed]

Hu, S.

Jiao, S.

Ku, G.

G. Ku, K. Maslov, L. Li, and L. V. Wang, J. Biomed. Opt. 15, 09322SSR (2010).
[CrossRef]

X. Wang, Y. Pang, G. Ku, G. Stoica, and L. V. Wang, Opt. Lett. 28, 1739 (2003).
[CrossRef] [PubMed]

L. V. Wang, X. Zhao, H. Sun, and G. Ku, Rev. Sci. Instrum. 70, 3744 (1999).
[CrossRef]

Kuppermann, B. D.

B. Rao, L. Yu, H. K. Chiang, L. C. Zacharias, R. M. Kurtz, B. D. Kuppermann, and Z. Chen, J. Biomed. Opt. 13, 040505 (2008).
[CrossRef] [PubMed]

Kurtz, R. M.

B. Rao, L. Yu, H. K. Chiang, L. C. Zacharias, R. M. Kurtz, B. D. Kuppermann, and Z. Chen, J. Biomed. Opt. 13, 040505 (2008).
[CrossRef] [PubMed]

Li, L.

G. Ku, K. Maslov, L. Li, and L. V. Wang, J. Biomed. Opt. 15, 09322SSR (2010).
[CrossRef]

Maslov, K.

Pang, Y.

Puliafito, C. A.

Rao, B.

B. Rao, L. Yu, H. K. Chiang, L. C. Zacharias, R. M. Kurtz, B. D. Kuppermann, and Z. Chen, J. Biomed. Opt. 13, 040505 (2008).
[CrossRef] [PubMed]

Stoica, G.

Sun, H.

L. V. Wang, X. Zhao, H. Sun, and G. Ku, Rev. Sci. Instrum. 70, 3744 (1999).
[CrossRef]

Wang, L.

L. Wang, Nature Photon. 3, 503 (2009).
[CrossRef]

Wang, L. V.

Wang, X.

Xie, Z.

Yu, L.

B. Rao, L. Yu, H. K. Chiang, L. C. Zacharias, R. M. Kurtz, B. D. Kuppermann, and Z. Chen, J. Biomed. Opt. 13, 040505 (2008).
[CrossRef] [PubMed]

Zacharias, L. C.

B. Rao, L. Yu, H. K. Chiang, L. C. Zacharias, R. M. Kurtz, B. D. Kuppermann, and Z. Chen, J. Biomed. Opt. 13, 040505 (2008).
[CrossRef] [PubMed]

Zhang, H. F.

Zhao, X.

L. V. Wang, X. Zhao, H. Sun, and G. Ku, Rev. Sci. Instrum. 70, 3744 (1999).
[CrossRef]

J. Biomed. Opt. (2)

B. Rao, L. Yu, H. K. Chiang, L. C. Zacharias, R. M. Kurtz, B. D. Kuppermann, and Z. Chen, J. Biomed. Opt. 13, 040505 (2008).
[CrossRef] [PubMed]

G. Ku, K. Maslov, L. Li, and L. V. Wang, J. Biomed. Opt. 15, 09322SSR (2010).
[CrossRef]

Med. Phys. (1)

L. V. Wang, Med. Phys. 35, 5758 (2008).
[CrossRef]

Nature Photon. (1)

L. Wang, Nature Photon. 3, 503 (2009).
[CrossRef]

Opt. Lett. (4)

Rev. Sci. Instrum. (1)

L. V. Wang, X. Zhao, H. Sun, and G. Ku, Rev. Sci. Instrum. 70, 3744 (1999).
[CrossRef]

Other (1)

Laser Institute of America, American National Standard for Safe Use of Lasers ANSI Z136.1-2000 (American National Standards Institute, Inc., 2000).

Supplementary Material (2)

» Media 1: MPG (4839 KB)     
» Media 2: MPG (3435 KB)     

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Figures (5)

Fig. 1
Fig. 1

Schematic of the hybrid-scanning optical-resolution photoacoustic microscopy system.

Fig. 2
Fig. 2

(A) Light microscopy image and (B) maximum amplitude projection PA image of the 6th and 7th groups in a USAF-1951 resolution target acquired by the hybrid-scanning optical-resolution photoacoustic microscopy system. The second element [identified by an arrow (red online)] in the 7th group was resolved in the PA image.

Fig. 3
Fig. 3

(A) B-mode PA image ( 1.5 mm × 4.0 mm ) scanning on the back side of an alignment plate (LCPA1, Thorlabs). (B) Peak PA signal amplitude versus depth.

Fig. 4
Fig. 4

Maximum amplitude projection PA images of the iris vasculature in a mouse (A) under dim ambient illumination condition and (C) bright ambient light illumination condition. Both the major artery circle (AC) and the minor artery circle are shown in the iris with larger pupil size in dim ambient light illumination condition. Their rendered 3D images are shown in (B) and (D) accordingly, and their 3D movies are provided in supplemental files Media 1 and Media 2.

Fig. 5
Fig. 5

(A) Photograph of a live mouse ear. (B) Maximum amplitude projection PA image of the mouse ear vasculature acquired in vivo.

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