Abstract

We have developed a common-path multimodal optical microscopy system that is capable of using a single optical source and a single camera to image amplitude, phase, and fluorescence features of a biological specimen. This is achieved by varying either contrast enhancement filters at the Fourier plane and/or neutral density/fluorescence filters in front of the CCD camera. The feasibility of the technique is demonstrated by obtaining brightfield, fluorescence, phase-contrast, spatially filtered, brightfield+fluorescence, phase+fluorescence, and edge-enhanced+fluorescence images of the same Drosophila embryo without the need for image registration and fusion. This comprehensive microscope has the capability of providing both structural and functional information and may be used for applications such as studying live-cell dynamics and in high throughput microscopy and automated microscopy.

© 2009 Optical Society of America

Full Article  |  PDF Article
OSA Recommended Articles
Multi-view image fusion improves resolution in three-dimensional microscopy

Jim Swoger, Peter Verveer, Klaus Greger, Jan Huisken, and Ernst H.K. Stelzer
Opt. Express 15(13) 8029-8042 (2007)

Open-top selective plane illumination microscope for conventionally mounted specimens

Ryan McGorty, Harrison Liu, Daichi Kamiyama, Zhiqiang Dong, Su Guo, and Bo Huang
Opt. Express 23(12) 16142-16153 (2015)

MultiFocus Polarization Microscope (MF-PolScope) for 3D polarization imaging of up to 25 focal planes simultaneously

Sara Abrahamsson, Molly McQuilken, Shalin B. Mehta, Amitabh Verma, Johannes Larsch, Rob Ilic, Rainer Heintzmann, Cornelia I. Bargmann, Amy S. Gladfelter, and Rudolf Oldenbourg
Opt. Express 23(6) 7734-7754 (2015)

References

You do not have subscription access to this journal. Citation lists with outbound citation links are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription

Cited By

You do not have subscription access to this journal. Cited by links are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription

Figures (1)

You do not have subscription access to this journal. Figure files are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription

Metrics

You do not have subscription access to this journal. Article level metrics are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription