Abstract

In in-line holographic microscopy a pinhole illuminates an object and a CCD-detector directly records the hologram in a pixel-pitch-dependent distance. A rapidly calculating exact reconstruction technique using a reorganized hologram with a low number of pixels, the tile superposition technique, is presented. The algorithm is applied on imaging of a 2μm bead cluster, and it is compared with other reconstruction techniques. The high-contrast image corresponds to an NA of 0.7. A full 4  megapixel reconstruction with a resolution approaching the diffraction limit is possible in less than a minute. The technique is a base for high-resolution wide-field imaging by multispot illumination.

© 2009 Optical Society of America

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References

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    [CrossRef] [PubMed]
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    [CrossRef]
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  9. R. Riesenberg, M. Kanka, and J. Bergmann, Proc. SPIE 6630, 66300I (2007).
    [CrossRef]

2008 (2)

2007 (1)

R. Riesenberg, M. Kanka, and J. Bergmann, Proc. SPIE 6630, 66300I (2007).
[CrossRef]

1999 (1)

1992 (1)

H. J. Kreuzer, K. Nakamura, A. Wierzbicki, H.-W. Fink, and H. Schmid, Ultramicroscopy 45, 381 (1992).
[CrossRef]

1948 (1)

D. Gabor, Nature 161, 777 (1948).
[CrossRef] [PubMed]

Alvarez-Palacio, D. C.

Bergmann, J.

R. Riesenberg, M. Kanka, and J. Bergmann, Proc. SPIE 6630, 66300I (2007).
[CrossRef]

R. Riesenberg, M. Kanka, and J. Bergmann, in Proceedings of the Deutsche Gesellschaft für Angewandte Optik (2006).

Fink, H.-W.

H. J. Kreuzer, K. Nakamura, A. Wierzbicki, H.-W. Fink, and H. Schmid, Ultramicroscopy 45, 381 (1992).
[CrossRef]

Gabor, D.

D. Gabor, Nature 161, 777 (1948).
[CrossRef] [PubMed]

Garcia-Sucerquia, J.

Goodman, J. W.

J. W. Goodman, Introduction to Fourier Optics (McGraw-Hill, 1996).

Kanka, M.

R. Riesenberg, M. Kanka, and J. Bergmann, Proc. SPIE 6630, 66300I (2007).
[CrossRef]

R. Riesenberg, M. Kanka, and J. Bergmann, in Proceedings of the Deutsche Gesellschaft für Angewandte Optik (2006).

Kreuzer, H. J.

J. Garcia-Sucerquia, D. C. Alvarez-Palacio, and H. J. Kreuzer, Appl. Opt. 47, 4723 (2008).
[CrossRef] [PubMed]

H. J. Kreuzer, K. Nakamura, A. Wierzbicki, H.-W. Fink, and H. Schmid, Ultramicroscopy 45, 381 (1992).
[CrossRef]

Nakamura, K.

H. J. Kreuzer, K. Nakamura, A. Wierzbicki, H.-W. Fink, and H. Schmid, Ultramicroscopy 45, 381 (1992).
[CrossRef]

Ohzu, H.

Osten, W.

Pedrini, G.

Riesenberg, R.

R. Riesenberg, M. Kanka, and J. Bergmann, Proc. SPIE 6630, 66300I (2007).
[CrossRef]

R. Riesenberg, M. Kanka, and J. Bergmann, in Proceedings of the Deutsche Gesellschaft für Angewandte Optik (2006).

Schmid, H.

H. J. Kreuzer, K. Nakamura, A. Wierzbicki, H.-W. Fink, and H. Schmid, Ultramicroscopy 45, 381 (1992).
[CrossRef]

Takaki, Y.

Wang, D.

Wierzbicki, A.

H. J. Kreuzer, K. Nakamura, A. Wierzbicki, H.-W. Fink, and H. Schmid, Ultramicroscopy 45, 381 (1992).
[CrossRef]

Yaroslavsky, L.

L. Yaroslavsky, Digital Holography and Digital Image Processing: Principles, Methods, Algorithms (Kluwer Academic, 2004).

Zhang, F.

Zhao, J.

Appl. Opt. (3)

Nature (1)

D. Gabor, Nature 161, 777 (1948).
[CrossRef] [PubMed]

Proc. SPIE (1)

R. Riesenberg, M. Kanka, and J. Bergmann, Proc. SPIE 6630, 66300I (2007).
[CrossRef]

Ultramicroscopy (1)

H. J. Kreuzer, K. Nakamura, A. Wierzbicki, H.-W. Fink, and H. Schmid, Ultramicroscopy 45, 381 (1992).
[CrossRef]

Other (3)

R. Riesenberg, M. Kanka, and J. Bergmann, in Proceedings of the Deutsche Gesellschaft für Angewandte Optik (2006).

J. W. Goodman, Introduction to Fourier Optics (McGraw-Hill, 1996).

L. Yaroslavsky, Digital Holography and Digital Image Processing: Principles, Methods, Algorithms (Kluwer Academic, 2004).

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Figures (3)

Fig. 1
Fig. 1

In-line holographic microscopy.

Fig. 2
Fig. 2

Scheme of the tile superposition technique for hologram reconstruction.

Fig. 3
Fig. 3

Reconstructions of transparent 2 μ m beads by a microscopic in-line holographic setup. (a) The Fresnel approximation cannot resolve the beads. (b) The DIHM algorithm based on the Kirchhoff–Helmholtz transform resolves most of the beads. (c) The proposed tile superposition propagation delivers high-contrast imaging corresponding to the used detection NA of 0.7.

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