Abstract

We present first results on a method enabling mechanical scanning-free tomography with submicrometer axial resolution by multiple-wavelength digital holographic microscopy. By sequentially acquiring reflection holograms and summing 20 wavefronts equally spaced in spatial frequency in the 485670nm range, we are able to achieve a slice-by-slice tomographic reconstruction with a 0.61μm axial resolution in a biological medium. The method is applied to erythrocytes investigation to retrieve the cellular membrane profile in three dimensions.

© 2009 Optical Society of America

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2008 (4)

B. Rappaz, A. Barbul, Y. Emery, R. Korenstein, C. Depeursinge, P. J. Magistretti, and P. Marquet, Cytometry, Part A 73A, 895 (2008).
[CrossRef]

M. Debailleul, B. Simon, V. Georges, O. Haeberlé, and V. Lauer, Meas. Sci. Technol. 19, 074009 (2008).
[CrossRef]

M. C. Potcoava and M. K. Kim, Meas. Sci. Technol. 19, 074010 (2008).
[CrossRef]

B. Rappaz, F. Charrière, C. Depeursinge, P. J. Magistretti, and P. Marquet, Opt. Lett. 33, 744 (2008).
[CrossRef] [PubMed]

2007 (2)

L. Yu and Z. Chen, Opt. Express 15, 878 (2007).
[CrossRef] [PubMed]

W. Choi, C. Fang-Yen, K. Badizadegan, S. Oh, N. Lue, R. R. Dasari, and M. S. Feld, Nat. Methods 4, 717 (2007).
[CrossRef] [PubMed]

2006 (4)

2005 (4)

2000 (1)

Aspert, N.

Badizadegan, K.

W. Choi, C. Fang-Yen, K. Badizadegan, S. Oh, N. Lue, R. R. Dasari, and M. S. Feld, Nat. Methods 4, 717 (2007).
[CrossRef] [PubMed]

G. Popescu, T. Ikeda, C. Best, K. Badizadegan, R. Dasari, and M. Feld, J. Biomed. Opt. 10, 060503 (2005).
[CrossRef]

Barbul, A.

B. Rappaz, A. Barbul, Y. Emery, R. Korenstein, C. Depeursinge, P. J. Magistretti, and P. Marquet, Cytometry, Part A 73A, 895 (2008).
[CrossRef]

Best, C.

G. Popescu, T. Ikeda, C. Best, K. Badizadegan, R. Dasari, and M. Feld, J. Biomed. Opt. 10, 060503 (2005).
[CrossRef]

Bredebusch, I.

B. Kemper, D. Carl, J. Schnekenburger, I. Bredebusch, M. Schafer, W. Domschke, and G. von Bally, J. Biomed. Opt. 11, 034005 (2006).
[CrossRef]

Carl, D.

B. Kemper, D. Carl, J. Schnekenburger, I. Bredebusch, M. Schafer, W. Domschke, and G. von Bally, J. Biomed. Opt. 11, 034005 (2006).
[CrossRef]

Charrière, F.

Chen, Z.

Choi, W.

W. Choi, C. Fang-Yen, K. Badizadegan, S. Oh, N. Lue, R. R. Dasari, and M. S. Feld, Nat. Methods 4, 717 (2007).
[CrossRef] [PubMed]

Colomb, T.

Cuche, E.

Dasari, R.

G. Popescu, T. Ikeda, C. Best, K. Badizadegan, R. Dasari, and M. Feld, J. Biomed. Opt. 10, 060503 (2005).
[CrossRef]

Dasari, R. R.

W. Choi, C. Fang-Yen, K. Badizadegan, S. Oh, N. Lue, R. R. Dasari, and M. S. Feld, Nat. Methods 4, 717 (2007).
[CrossRef] [PubMed]

Debailleul, M.

M. Debailleul, B. Simon, V. Georges, O. Haeberlé, and V. Lauer, Meas. Sci. Technol. 19, 074009 (2008).
[CrossRef]

Depeursinge, C.

Domschke, W.

B. Kemper, D. Carl, J. Schnekenburger, I. Bredebusch, M. Schafer, W. Domschke, and G. von Bally, J. Biomed. Opt. 11, 034005 (2006).
[CrossRef]

Emery, Y.

Fang-Yen, C.

W. Choi, C. Fang-Yen, K. Badizadegan, S. Oh, N. Lue, R. R. Dasari, and M. S. Feld, Nat. Methods 4, 717 (2007).
[CrossRef] [PubMed]

Feld, M.

G. Popescu, T. Ikeda, C. Best, K. Badizadegan, R. Dasari, and M. Feld, J. Biomed. Opt. 10, 060503 (2005).
[CrossRef]

Feld, M. S.

W. Choi, C. Fang-Yen, K. Badizadegan, S. Oh, N. Lue, R. R. Dasari, and M. S. Feld, Nat. Methods 4, 717 (2007).
[CrossRef] [PubMed]

Georges, V.

M. Debailleul, B. Simon, V. Georges, O. Haeberlé, and V. Lauer, Meas. Sci. Technol. 19, 074009 (2008).
[CrossRef]

Haeberlé, O.

M. Debailleul, B. Simon, V. Georges, O. Haeberlé, and V. Lauer, Meas. Sci. Technol. 19, 074009 (2008).
[CrossRef]

Herminjard, S.

Ikeda, T.

G. Popescu, T. Ikeda, C. Best, K. Badizadegan, R. Dasari, and M. Feld, J. Biomed. Opt. 10, 060503 (2005).
[CrossRef]

Kemper, B.

B. Kemper, D. Carl, J. Schnekenburger, I. Bredebusch, M. Schafer, W. Domschke, and G. von Bally, J. Biomed. Opt. 11, 034005 (2006).
[CrossRef]

Kim, M. K.

M. C. Potcoava and M. K. Kim, Meas. Sci. Technol. 19, 074010 (2008).
[CrossRef]

M. K. Kim, Opt. Express 7, 305 (2000).
[CrossRef] [PubMed]

Korenstein, R.

B. Rappaz, A. Barbul, Y. Emery, R. Korenstein, C. Depeursinge, P. J. Magistretti, and P. Marquet, Cytometry, Part A 73A, 895 (2008).
[CrossRef]

Kühn, J.

Lathi, B.

B. Lathi, Signal Processing and Linear System (Berkeley-Cambridge, 1998).

Lauer, V.

M. Debailleul, B. Simon, V. Georges, O. Haeberlé, and V. Lauer, Meas. Sci. Technol. 19, 074009 (2008).
[CrossRef]

Lue, N.

W. Choi, C. Fang-Yen, K. Badizadegan, S. Oh, N. Lue, R. R. Dasari, and M. S. Feld, Nat. Methods 4, 717 (2007).
[CrossRef] [PubMed]

Magistretti, P. J.

Marian, A.

Marquet, P.

Martina, M.

F. Moritz and M. Martina, J. Biomed. Opt. 10, 064019 (2005).
[CrossRef]

Montfort, F.

Moritz, F.

F. Moritz and M. Martina, J. Biomed. Opt. 10, 064019 (2005).
[CrossRef]

Oh, S.

W. Choi, C. Fang-Yen, K. Badizadegan, S. Oh, N. Lue, R. R. Dasari, and M. S. Feld, Nat. Methods 4, 717 (2007).
[CrossRef] [PubMed]

Popescu, G.

G. Popescu, T. Ikeda, C. Best, K. Badizadegan, R. Dasari, and M. Feld, J. Biomed. Opt. 10, 060503 (2005).
[CrossRef]

Potcoava, M. C.

M. C. Potcoava and M. K. Kim, Meas. Sci. Technol. 19, 074010 (2008).
[CrossRef]

Rappaz, B.

Schafer, M.

B. Kemper, D. Carl, J. Schnekenburger, I. Bredebusch, M. Schafer, W. Domschke, and G. von Bally, J. Biomed. Opt. 11, 034005 (2006).
[CrossRef]

Schnekenburger, J.

B. Kemper, D. Carl, J. Schnekenburger, I. Bredebusch, M. Schafer, W. Domschke, and G. von Bally, J. Biomed. Opt. 11, 034005 (2006).
[CrossRef]

Simon, B.

M. Debailleul, B. Simon, V. Georges, O. Haeberlé, and V. Lauer, Meas. Sci. Technol. 19, 074009 (2008).
[CrossRef]

von Bally, G.

B. Kemper, D. Carl, J. Schnekenburger, I. Bredebusch, M. Schafer, W. Domschke, and G. von Bally, J. Biomed. Opt. 11, 034005 (2006).
[CrossRef]

Yu, L.

Appl. Opt. (2)

Cytometry, Part A (1)

B. Rappaz, A. Barbul, Y. Emery, R. Korenstein, C. Depeursinge, P. J. Magistretti, and P. Marquet, Cytometry, Part A 73A, 895 (2008).
[CrossRef]

J. Biomed. Opt. (3)

B. Kemper, D. Carl, J. Schnekenburger, I. Bredebusch, M. Schafer, W. Domschke, and G. von Bally, J. Biomed. Opt. 11, 034005 (2006).
[CrossRef]

G. Popescu, T. Ikeda, C. Best, K. Badizadegan, R. Dasari, and M. Feld, J. Biomed. Opt. 10, 060503 (2005).
[CrossRef]

F. Moritz and M. Martina, J. Biomed. Opt. 10, 064019 (2005).
[CrossRef]

Meas. Sci. Technol. (2)

M. C. Potcoava and M. K. Kim, Meas. Sci. Technol. 19, 074010 (2008).
[CrossRef]

M. Debailleul, B. Simon, V. Georges, O. Haeberlé, and V. Lauer, Meas. Sci. Technol. 19, 074009 (2008).
[CrossRef]

Nat. Methods (1)

W. Choi, C. Fang-Yen, K. Badizadegan, S. Oh, N. Lue, R. R. Dasari, and M. S. Feld, Nat. Methods 4, 717 (2007).
[CrossRef] [PubMed]

Opt. Express (3)

Opt. Lett. (3)

Other (1)

B. Lathi, Signal Processing and Linear System (Berkeley-Cambridge, 1998).

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Figures (4)

Fig. 1
Fig. 1

Nominal filter function T ( r P Q ) for 20 equally k-spaced wavelengths in the 485 670 nm range.

Fig. 2
Fig. 2

Experimental configuration. (a) Optical setup. BS, beam splitter; M, mirror; DL, delay line; BK7, BK7 glass block; BE, beam-expander; L, lens; MO, microscope objective; S, specimen. (b) Sample arrangement.

Fig. 3
Fig. 3

Results for two different RBCs. (a) and (b) Reflection DHM phase images; (c) and (d) tomographic X Z cuts through the cell along the dashed curve in (a) and (b) with no weighting function. (e) and (f) Same as (c) and (d) but with an α = 3.8 Kaiser weighting function; (g) and (h) peak-detected results applied on a zoomed region (white dashed rectangle) of (e) and (f).

Fig. 4
Fig. 4

(a) 3D representation of the series of peak-detected tomographic cuts as in Fig. 3g on the RBC of Fig. 3a; (b) lateral view of (a).

Equations (5)

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Ψ ( Q ) V A ( P ) e i k ¯ r P Q T ( r P Q ) d 3 r P Q ,
T ( r P Q ) = sin ( Δ k r P Q N 2 ) sin ( Δ k r P Q 1 2 ) .
Ψ ( Q ) = j = 0 N 1 w j Ψ j ( Q ) ,
Ψ ( x , y , z ) = j = 0 N 1 w j Ψ j ( x , y , 0 ) Ψ j ( x , y , 0 ) e i φ j e i φ z ,
w ( k ) = J 0 ( α 1 4 ( k N Δ k ) 2 ) J 0 ( α ) ,

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