Abstract

Fourier transform second-harmonic generation (SHG) microscopy has been applied to quantitatively compare the information content between SHG images obtained from the forward and backward direction for three tissue types: porcine tendon, sclera, and ear cartilage. Both signal types yield consistent information on the preferred orientation of collagen fibers. For all specimens, the Fourier transform of the forward and backward SHG images produces several overlapping peaks in the magnitude spectrum at various depths into the tissues, indicating that some information present in the forward SHG images can be extracted from the backward SHG images. This study highlights the potential of backward SHG microscopy for medical diagnostics.

© 2009 Optical Society of America

Full Article  |  PDF Article

References

  • View by:
  • |
  • |
  • |

  1. A. Zoumi, A. Yeh, and B. J. Tromberg, Proc. Natl. Acad. Sci. USA 99, 11014 (2002).
    [CrossRef] [PubMed]
  2. A. H. Reshak, J. Microsc. 234, 280 (2009).
    [CrossRef] [PubMed]
  3. L. Moreaux, O. Sandre, M. Blanchard-Desce, and J. Mertz, Opt. Lett. 25, 320 (2000).
    [CrossRef]
  4. F. Tiaho, G. Recher, and D. Rouede, Opt. Express 15, 12286 (2007).
    [CrossRef] [PubMed]
  5. N. Morishige, A. Wahlert, M. Kenney, D. Brown, K. Kawamoto, T. Chikama, T. Nishida, and J. V. Jester, Invest. Ophthalmol. Visual Sci. 48, 1087 (2007).
    [CrossRef]
  6. J. Omens, T. R. Miller, and J. W. Covell, Cardiovasc. Res. 33, 351 (1997).
    [CrossRef] [PubMed]
  7. S. V. Plotnikov, A. M. Kenny, S. J. Walsh, B. Zubrowski, C. Joseph, V. L. Scranton, G. A. Kuchel, D. Dauser, M. S. Xu, C. C. Pilbeam, D. J. Adams, R. P. Dougherty, P. J. Campagnola, and W. A. Mohler, J. Biomed. Opt. 13, 11 (2008).
    [CrossRef]
  8. A. H. Reshak, Micron 40, 750 (2009).
    [CrossRef] [PubMed]
  9. P. J. Campagnola and L. M. Loew, Nat. Biotechnol. 21, 1356 (2003).
    [CrossRef] [PubMed]
  10. R. M. Williams, W. R. Zipfel, and W. W. Webb, Biophys. J. 88, 1377 (2005).
    [CrossRef]
  11. P. Bianchini and A. Diaspro, J. Biophotonics 1, 443 (2008).
    [CrossRef]
  12. F. Legare, C. Pfeffer, and B. R. Olsen, Biophys. J. 93, 1312 (2007).
    [CrossRef] [PubMed]
  13. K. Konig, J. Biophotonics 1, 13 (2008).
    [CrossRef]
  14. H. Wen Lo, M. Lin, C. Hsueh, W. Chen, S. Lin, and S. Jee, Proc. SPIE 6860, 68600F1 (2008).
  15. S. W. Chu, J. Med. Biol. Eng. 27, 177 (2007).
  16. R. Ambekar Ramachandra Rao, M. R. Mehta, and K. C. Toussaint, Jr., Opt. Express 17, 14534 (2009).
    [CrossRef] [PubMed]
  17. M. Han, G. Giese, and J. F. Bille, Opt. Express 13, 5791 (2005).
    [CrossRef] [PubMed]

2009

2008

K. Konig, J. Biophotonics 1, 13 (2008).
[CrossRef]

H. Wen Lo, M. Lin, C. Hsueh, W. Chen, S. Lin, and S. Jee, Proc. SPIE 6860, 68600F1 (2008).

S. V. Plotnikov, A. M. Kenny, S. J. Walsh, B. Zubrowski, C. Joseph, V. L. Scranton, G. A. Kuchel, D. Dauser, M. S. Xu, C. C. Pilbeam, D. J. Adams, R. P. Dougherty, P. J. Campagnola, and W. A. Mohler, J. Biomed. Opt. 13, 11 (2008).
[CrossRef]

P. Bianchini and A. Diaspro, J. Biophotonics 1, 443 (2008).
[CrossRef]

2007

F. Legare, C. Pfeffer, and B. R. Olsen, Biophys. J. 93, 1312 (2007).
[CrossRef] [PubMed]

N. Morishige, A. Wahlert, M. Kenney, D. Brown, K. Kawamoto, T. Chikama, T. Nishida, and J. V. Jester, Invest. Ophthalmol. Visual Sci. 48, 1087 (2007).
[CrossRef]

S. W. Chu, J. Med. Biol. Eng. 27, 177 (2007).

F. Tiaho, G. Recher, and D. Rouede, Opt. Express 15, 12286 (2007).
[CrossRef] [PubMed]

2005

M. Han, G. Giese, and J. F. Bille, Opt. Express 13, 5791 (2005).
[CrossRef] [PubMed]

R. M. Williams, W. R. Zipfel, and W. W. Webb, Biophys. J. 88, 1377 (2005).
[CrossRef]

2003

P. J. Campagnola and L. M. Loew, Nat. Biotechnol. 21, 1356 (2003).
[CrossRef] [PubMed]

2002

A. Zoumi, A. Yeh, and B. J. Tromberg, Proc. Natl. Acad. Sci. USA 99, 11014 (2002).
[CrossRef] [PubMed]

2000

1997

J. Omens, T. R. Miller, and J. W. Covell, Cardiovasc. Res. 33, 351 (1997).
[CrossRef] [PubMed]

Adams, D. J.

S. V. Plotnikov, A. M. Kenny, S. J. Walsh, B. Zubrowski, C. Joseph, V. L. Scranton, G. A. Kuchel, D. Dauser, M. S. Xu, C. C. Pilbeam, D. J. Adams, R. P. Dougherty, P. J. Campagnola, and W. A. Mohler, J. Biomed. Opt. 13, 11 (2008).
[CrossRef]

Bianchini, P.

P. Bianchini and A. Diaspro, J. Biophotonics 1, 443 (2008).
[CrossRef]

Bille, J. F.

Blanchard-Desce, M.

Brown, D.

N. Morishige, A. Wahlert, M. Kenney, D. Brown, K. Kawamoto, T. Chikama, T. Nishida, and J. V. Jester, Invest. Ophthalmol. Visual Sci. 48, 1087 (2007).
[CrossRef]

Campagnola, P. J.

S. V. Plotnikov, A. M. Kenny, S. J. Walsh, B. Zubrowski, C. Joseph, V. L. Scranton, G. A. Kuchel, D. Dauser, M. S. Xu, C. C. Pilbeam, D. J. Adams, R. P. Dougherty, P. J. Campagnola, and W. A. Mohler, J. Biomed. Opt. 13, 11 (2008).
[CrossRef]

P. J. Campagnola and L. M. Loew, Nat. Biotechnol. 21, 1356 (2003).
[CrossRef] [PubMed]

Chen, W.

H. Wen Lo, M. Lin, C. Hsueh, W. Chen, S. Lin, and S. Jee, Proc. SPIE 6860, 68600F1 (2008).

Chikama, T.

N. Morishige, A. Wahlert, M. Kenney, D. Brown, K. Kawamoto, T. Chikama, T. Nishida, and J. V. Jester, Invest. Ophthalmol. Visual Sci. 48, 1087 (2007).
[CrossRef]

Chu, S. W.

S. W. Chu, J. Med. Biol. Eng. 27, 177 (2007).

Covell, J. W.

J. Omens, T. R. Miller, and J. W. Covell, Cardiovasc. Res. 33, 351 (1997).
[CrossRef] [PubMed]

Dauser, D.

S. V. Plotnikov, A. M. Kenny, S. J. Walsh, B. Zubrowski, C. Joseph, V. L. Scranton, G. A. Kuchel, D. Dauser, M. S. Xu, C. C. Pilbeam, D. J. Adams, R. P. Dougherty, P. J. Campagnola, and W. A. Mohler, J. Biomed. Opt. 13, 11 (2008).
[CrossRef]

Diaspro, A.

P. Bianchini and A. Diaspro, J. Biophotonics 1, 443 (2008).
[CrossRef]

Dougherty, R. P.

S. V. Plotnikov, A. M. Kenny, S. J. Walsh, B. Zubrowski, C. Joseph, V. L. Scranton, G. A. Kuchel, D. Dauser, M. S. Xu, C. C. Pilbeam, D. J. Adams, R. P. Dougherty, P. J. Campagnola, and W. A. Mohler, J. Biomed. Opt. 13, 11 (2008).
[CrossRef]

Giese, G.

Han, M.

Hsueh, C.

H. Wen Lo, M. Lin, C. Hsueh, W. Chen, S. Lin, and S. Jee, Proc. SPIE 6860, 68600F1 (2008).

Jee, S.

H. Wen Lo, M. Lin, C. Hsueh, W. Chen, S. Lin, and S. Jee, Proc. SPIE 6860, 68600F1 (2008).

Jester, J. V.

N. Morishige, A. Wahlert, M. Kenney, D. Brown, K. Kawamoto, T. Chikama, T. Nishida, and J. V. Jester, Invest. Ophthalmol. Visual Sci. 48, 1087 (2007).
[CrossRef]

Joseph, C.

S. V. Plotnikov, A. M. Kenny, S. J. Walsh, B. Zubrowski, C. Joseph, V. L. Scranton, G. A. Kuchel, D. Dauser, M. S. Xu, C. C. Pilbeam, D. J. Adams, R. P. Dougherty, P. J. Campagnola, and W. A. Mohler, J. Biomed. Opt. 13, 11 (2008).
[CrossRef]

Kawamoto, K.

N. Morishige, A. Wahlert, M. Kenney, D. Brown, K. Kawamoto, T. Chikama, T. Nishida, and J. V. Jester, Invest. Ophthalmol. Visual Sci. 48, 1087 (2007).
[CrossRef]

Kenney, M.

N. Morishige, A. Wahlert, M. Kenney, D. Brown, K. Kawamoto, T. Chikama, T. Nishida, and J. V. Jester, Invest. Ophthalmol. Visual Sci. 48, 1087 (2007).
[CrossRef]

Kenny, A. M.

S. V. Plotnikov, A. M. Kenny, S. J. Walsh, B. Zubrowski, C. Joseph, V. L. Scranton, G. A. Kuchel, D. Dauser, M. S. Xu, C. C. Pilbeam, D. J. Adams, R. P. Dougherty, P. J. Campagnola, and W. A. Mohler, J. Biomed. Opt. 13, 11 (2008).
[CrossRef]

Konig, K.

K. Konig, J. Biophotonics 1, 13 (2008).
[CrossRef]

Kuchel, G. A.

S. V. Plotnikov, A. M. Kenny, S. J. Walsh, B. Zubrowski, C. Joseph, V. L. Scranton, G. A. Kuchel, D. Dauser, M. S. Xu, C. C. Pilbeam, D. J. Adams, R. P. Dougherty, P. J. Campagnola, and W. A. Mohler, J. Biomed. Opt. 13, 11 (2008).
[CrossRef]

Legare, F.

F. Legare, C. Pfeffer, and B. R. Olsen, Biophys. J. 93, 1312 (2007).
[CrossRef] [PubMed]

Lin, M.

H. Wen Lo, M. Lin, C. Hsueh, W. Chen, S. Lin, and S. Jee, Proc. SPIE 6860, 68600F1 (2008).

Lin, S.

H. Wen Lo, M. Lin, C. Hsueh, W. Chen, S. Lin, and S. Jee, Proc. SPIE 6860, 68600F1 (2008).

Lo, H. Wen

H. Wen Lo, M. Lin, C. Hsueh, W. Chen, S. Lin, and S. Jee, Proc. SPIE 6860, 68600F1 (2008).

Loew, L. M.

P. J. Campagnola and L. M. Loew, Nat. Biotechnol. 21, 1356 (2003).
[CrossRef] [PubMed]

Mehta, M. R.

Mertz, J.

Miller, T. R.

J. Omens, T. R. Miller, and J. W. Covell, Cardiovasc. Res. 33, 351 (1997).
[CrossRef] [PubMed]

Mohler, W. A.

S. V. Plotnikov, A. M. Kenny, S. J. Walsh, B. Zubrowski, C. Joseph, V. L. Scranton, G. A. Kuchel, D. Dauser, M. S. Xu, C. C. Pilbeam, D. J. Adams, R. P. Dougherty, P. J. Campagnola, and W. A. Mohler, J. Biomed. Opt. 13, 11 (2008).
[CrossRef]

Moreaux, L.

Morishige, N.

N. Morishige, A. Wahlert, M. Kenney, D. Brown, K. Kawamoto, T. Chikama, T. Nishida, and J. V. Jester, Invest. Ophthalmol. Visual Sci. 48, 1087 (2007).
[CrossRef]

Nishida, T.

N. Morishige, A. Wahlert, M. Kenney, D. Brown, K. Kawamoto, T. Chikama, T. Nishida, and J. V. Jester, Invest. Ophthalmol. Visual Sci. 48, 1087 (2007).
[CrossRef]

Olsen, B. R.

F. Legare, C. Pfeffer, and B. R. Olsen, Biophys. J. 93, 1312 (2007).
[CrossRef] [PubMed]

Omens, J.

J. Omens, T. R. Miller, and J. W. Covell, Cardiovasc. Res. 33, 351 (1997).
[CrossRef] [PubMed]

Pfeffer, C.

F. Legare, C. Pfeffer, and B. R. Olsen, Biophys. J. 93, 1312 (2007).
[CrossRef] [PubMed]

Pilbeam, C. C.

S. V. Plotnikov, A. M. Kenny, S. J. Walsh, B. Zubrowski, C. Joseph, V. L. Scranton, G. A. Kuchel, D. Dauser, M. S. Xu, C. C. Pilbeam, D. J. Adams, R. P. Dougherty, P. J. Campagnola, and W. A. Mohler, J. Biomed. Opt. 13, 11 (2008).
[CrossRef]

Plotnikov, S. V.

S. V. Plotnikov, A. M. Kenny, S. J. Walsh, B. Zubrowski, C. Joseph, V. L. Scranton, G. A. Kuchel, D. Dauser, M. S. Xu, C. C. Pilbeam, D. J. Adams, R. P. Dougherty, P. J. Campagnola, and W. A. Mohler, J. Biomed. Opt. 13, 11 (2008).
[CrossRef]

Rao, R. Ambekar Ramachandra

Recher, G.

Reshak, A. H.

A. H. Reshak, Micron 40, 750 (2009).
[CrossRef] [PubMed]

A. H. Reshak, J. Microsc. 234, 280 (2009).
[CrossRef] [PubMed]

Rouede, D.

Sandre, O.

Scranton, V. L.

S. V. Plotnikov, A. M. Kenny, S. J. Walsh, B. Zubrowski, C. Joseph, V. L. Scranton, G. A. Kuchel, D. Dauser, M. S. Xu, C. C. Pilbeam, D. J. Adams, R. P. Dougherty, P. J. Campagnola, and W. A. Mohler, J. Biomed. Opt. 13, 11 (2008).
[CrossRef]

Tiaho, F.

Toussaint, K. C.

Tromberg, B. J.

A. Zoumi, A. Yeh, and B. J. Tromberg, Proc. Natl. Acad. Sci. USA 99, 11014 (2002).
[CrossRef] [PubMed]

Wahlert, A.

N. Morishige, A. Wahlert, M. Kenney, D. Brown, K. Kawamoto, T. Chikama, T. Nishida, and J. V. Jester, Invest. Ophthalmol. Visual Sci. 48, 1087 (2007).
[CrossRef]

Walsh, S. J.

S. V. Plotnikov, A. M. Kenny, S. J. Walsh, B. Zubrowski, C. Joseph, V. L. Scranton, G. A. Kuchel, D. Dauser, M. S. Xu, C. C. Pilbeam, D. J. Adams, R. P. Dougherty, P. J. Campagnola, and W. A. Mohler, J. Biomed. Opt. 13, 11 (2008).
[CrossRef]

Webb, W. W.

R. M. Williams, W. R. Zipfel, and W. W. Webb, Biophys. J. 88, 1377 (2005).
[CrossRef]

Williams, R. M.

R. M. Williams, W. R. Zipfel, and W. W. Webb, Biophys. J. 88, 1377 (2005).
[CrossRef]

Xu, M. S.

S. V. Plotnikov, A. M. Kenny, S. J. Walsh, B. Zubrowski, C. Joseph, V. L. Scranton, G. A. Kuchel, D. Dauser, M. S. Xu, C. C. Pilbeam, D. J. Adams, R. P. Dougherty, P. J. Campagnola, and W. A. Mohler, J. Biomed. Opt. 13, 11 (2008).
[CrossRef]

Yeh, A.

A. Zoumi, A. Yeh, and B. J. Tromberg, Proc. Natl. Acad. Sci. USA 99, 11014 (2002).
[CrossRef] [PubMed]

Zipfel, W. R.

R. M. Williams, W. R. Zipfel, and W. W. Webb, Biophys. J. 88, 1377 (2005).
[CrossRef]

Zoumi, A.

A. Zoumi, A. Yeh, and B. J. Tromberg, Proc. Natl. Acad. Sci. USA 99, 11014 (2002).
[CrossRef] [PubMed]

Zubrowski, B.

S. V. Plotnikov, A. M. Kenny, S. J. Walsh, B. Zubrowski, C. Joseph, V. L. Scranton, G. A. Kuchel, D. Dauser, M. S. Xu, C. C. Pilbeam, D. J. Adams, R. P. Dougherty, P. J. Campagnola, and W. A. Mohler, J. Biomed. Opt. 13, 11 (2008).
[CrossRef]

Biophys. J.

R. M. Williams, W. R. Zipfel, and W. W. Webb, Biophys. J. 88, 1377 (2005).
[CrossRef]

F. Legare, C. Pfeffer, and B. R. Olsen, Biophys. J. 93, 1312 (2007).
[CrossRef] [PubMed]

Cardiovasc. Res.

J. Omens, T. R. Miller, and J. W. Covell, Cardiovasc. Res. 33, 351 (1997).
[CrossRef] [PubMed]

Invest. Ophthalmol. Visual Sci.

N. Morishige, A. Wahlert, M. Kenney, D. Brown, K. Kawamoto, T. Chikama, T. Nishida, and J. V. Jester, Invest. Ophthalmol. Visual Sci. 48, 1087 (2007).
[CrossRef]

J. Biomed. Opt.

S. V. Plotnikov, A. M. Kenny, S. J. Walsh, B. Zubrowski, C. Joseph, V. L. Scranton, G. A. Kuchel, D. Dauser, M. S. Xu, C. C. Pilbeam, D. J. Adams, R. P. Dougherty, P. J. Campagnola, and W. A. Mohler, J. Biomed. Opt. 13, 11 (2008).
[CrossRef]

J. Biophotonics

K. Konig, J. Biophotonics 1, 13 (2008).
[CrossRef]

P. Bianchini and A. Diaspro, J. Biophotonics 1, 443 (2008).
[CrossRef]

J. Med. Biol. Eng.

S. W. Chu, J. Med. Biol. Eng. 27, 177 (2007).

J. Microsc.

A. H. Reshak, J. Microsc. 234, 280 (2009).
[CrossRef] [PubMed]

Micron

A. H. Reshak, Micron 40, 750 (2009).
[CrossRef] [PubMed]

Nat. Biotechnol.

P. J. Campagnola and L. M. Loew, Nat. Biotechnol. 21, 1356 (2003).
[CrossRef] [PubMed]

Opt. Express

Opt. Lett.

Proc. Natl. Acad. Sci. USA

A. Zoumi, A. Yeh, and B. J. Tromberg, Proc. Natl. Acad. Sci. USA 99, 11014 (2002).
[CrossRef] [PubMed]

Proc. SPIE

H. Wen Lo, M. Lin, C. Hsueh, W. Chen, S. Lin, and S. Jee, Proc. SPIE 6860, 68600F1 (2008).

Cited By

OSA participates in CrossRef's Cited-By Linking service. Citing articles from OSA journals and other participating publishers are listed here.

Alert me when this article is cited.


Figures (3)

Fig. 1
Fig. 1

Forward (left, red) and backward (right, green) SHG images of collagen fibers in the tendon, sclera, and ear cartilage at various depths within the sample. Scale bar: 15 μ m (same for all samples). The arrows represent the preferred orientation of the fibers within each region of interest A, B, and C.

Fig. 2
Fig. 2

Difference in the preferred orientation Δ γ of collagen fibers between forward and backward SHG versus depth for the tendon, the sclera, and the ear. The regions of interest are the same as those shown in Fig. 1.

Fig. 3
Fig. 3

Plots of spatial frequency peaks of the three regions of interest at various depths for the tendon, the sclera, and the ear. See text for details.

Metrics