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Quantitative measurements of absorption spectra in scattering media by low-coherence spectroscopy

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Abstract

Low-coherence spectroscopy (LCS) is a spectroscopic method that allows for quantitative and localized assessment of absorption spectra by combining reflection spectroscopy with low-coherence interferometry. We describe absorption coefficient (μa) measurements by LCS in tissue simulating phantoms with varying scattering and absorbing properties. We used LCS in the 455680nm wavelength range with a spectral resolution of 8nm to obtain μa spectra with ±0.5mm1 accuracy. We conclude that LCS is a promising technique for the in vivo determination of tissue chromophore concentrations.

© 2009 Optical Society of America

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