Abstract

We demonstrate image-based aberration correction in a third-harmonic generation (THG) microscope. We describe a robust, mostly sample-independent correction scheme relying on prior measurement of the influence of aberration modes produced by a deformable mirror on the quality of THG images. We find that using image sharpness as an image quality metric, correction of N aberration modes is achieved using 2(2N+1) measurements in a variety of samples. We also report aberration correction in combined multiharmonic and two-photon excited fluorescence experiments. Finally, we demonstrate time-dependent adaptive THG imaging in developing embryonic tissue.

© 2009 Optical Society of America

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  1. P. N. Marsh, D. Burns, and J. M. Girkin, Opt. Express 11, 1123 (2003).
  2. M. Rueckel, J. Mack-Bucher, and W. Denk, Proc. Natl. Acad. Sci. USA 103, 17137 (2006).
    [CrossRef]
  3. M. J. Booth, M. A. A. Neil, R. Juškaitis, and T. Wilson, Proc. Natl. Acad. Sci. USA 99, 5788 (2002).
    [CrossRef]
  4. D. Débarre, E. J. Botcherby, M. J. Booth, and T. Wilson, Opt. Express 16, 9290 (2008).
    [CrossRef]
  5. D. Débarre, E. J. Botcherby, T. Watanabe, S. Srinivas, M. J. Booth, and T. Wilson, Opt. Lett. 16, 2495 (2009).
    [CrossRef]
  6. Y. Barad, H. Eisenberg, M. Horowitz, and Y. Silberberg, Appl. Phys. Lett. 70, 922 (1997).
    [CrossRef]
  7. D. Débarre, W. Supatto, and E. Beaurepaire, Opt. Lett. 30, 2134 (2005).
    [CrossRef]
  8. N. Olivier and E. Beaurepaire, Opt. Express 16, 14703 (2008).
    [CrossRef]
  9. R. Herloski, J. Opt. Soc. Am. A 2, 1027 (1985).
    [CrossRef]
  10. D. Débarre, W. Supatto, E. Farge, B. Moulia, M.-C. Schanne-Klein, and E. Beaurepaire, Opt. Lett. 29, 2881 (2004).
    [CrossRef]

2009 (1)

D. Débarre, E. J. Botcherby, T. Watanabe, S. Srinivas, M. J. Booth, and T. Wilson, Opt. Lett. 16, 2495 (2009).
[CrossRef]

2008 (2)

2006 (1)

M. Rueckel, J. Mack-Bucher, and W. Denk, Proc. Natl. Acad. Sci. USA 103, 17137 (2006).
[CrossRef]

2005 (1)

2004 (1)

2003 (1)

2002 (1)

M. J. Booth, M. A. A. Neil, R. Juškaitis, and T. Wilson, Proc. Natl. Acad. Sci. USA 99, 5788 (2002).
[CrossRef]

1997 (1)

Y. Barad, H. Eisenberg, M. Horowitz, and Y. Silberberg, Appl. Phys. Lett. 70, 922 (1997).
[CrossRef]

1985 (1)

Barad, Y.

Y. Barad, H. Eisenberg, M. Horowitz, and Y. Silberberg, Appl. Phys. Lett. 70, 922 (1997).
[CrossRef]

Beaurepaire, E.

Booth, M. J.

D. Débarre, E. J. Botcherby, T. Watanabe, S. Srinivas, M. J. Booth, and T. Wilson, Opt. Lett. 16, 2495 (2009).
[CrossRef]

D. Débarre, E. J. Botcherby, M. J. Booth, and T. Wilson, Opt. Express 16, 9290 (2008).
[CrossRef]

M. J. Booth, M. A. A. Neil, R. Juškaitis, and T. Wilson, Proc. Natl. Acad. Sci. USA 99, 5788 (2002).
[CrossRef]

Botcherby, E. J.

D. Débarre, E. J. Botcherby, T. Watanabe, S. Srinivas, M. J. Booth, and T. Wilson, Opt. Lett. 16, 2495 (2009).
[CrossRef]

D. Débarre, E. J. Botcherby, M. J. Booth, and T. Wilson, Opt. Express 16, 9290 (2008).
[CrossRef]

Burns, D.

Débarre, D.

Denk, W.

M. Rueckel, J. Mack-Bucher, and W. Denk, Proc. Natl. Acad. Sci. USA 103, 17137 (2006).
[CrossRef]

Eisenberg, H.

Y. Barad, H. Eisenberg, M. Horowitz, and Y. Silberberg, Appl. Phys. Lett. 70, 922 (1997).
[CrossRef]

Farge, E.

Girkin, J. M.

Herloski, R.

Horowitz, M.

Y. Barad, H. Eisenberg, M. Horowitz, and Y. Silberberg, Appl. Phys. Lett. 70, 922 (1997).
[CrossRef]

Juškaitis, R.

M. J. Booth, M. A. A. Neil, R. Juškaitis, and T. Wilson, Proc. Natl. Acad. Sci. USA 99, 5788 (2002).
[CrossRef]

Mack-Bucher, J.

M. Rueckel, J. Mack-Bucher, and W. Denk, Proc. Natl. Acad. Sci. USA 103, 17137 (2006).
[CrossRef]

Marsh, P. N.

Moulia, B.

Neil, M. A. A.

M. J. Booth, M. A. A. Neil, R. Juškaitis, and T. Wilson, Proc. Natl. Acad. Sci. USA 99, 5788 (2002).
[CrossRef]

Olivier, N.

Rueckel, M.

M. Rueckel, J. Mack-Bucher, and W. Denk, Proc. Natl. Acad. Sci. USA 103, 17137 (2006).
[CrossRef]

Schanne-Klein, M.-C.

Silberberg, Y.

Y. Barad, H. Eisenberg, M. Horowitz, and Y. Silberberg, Appl. Phys. Lett. 70, 922 (1997).
[CrossRef]

Srinivas, S.

D. Débarre, E. J. Botcherby, T. Watanabe, S. Srinivas, M. J. Booth, and T. Wilson, Opt. Lett. 16, 2495 (2009).
[CrossRef]

Supatto, W.

Watanabe, T.

D. Débarre, E. J. Botcherby, T. Watanabe, S. Srinivas, M. J. Booth, and T. Wilson, Opt. Lett. 16, 2495 (2009).
[CrossRef]

Wilson, T.

D. Débarre, E. J. Botcherby, T. Watanabe, S. Srinivas, M. J. Booth, and T. Wilson, Opt. Lett. 16, 2495 (2009).
[CrossRef]

D. Débarre, E. J. Botcherby, M. J. Booth, and T. Wilson, Opt. Express 16, 9290 (2008).
[CrossRef]

M. J. Booth, M. A. A. Neil, R. Juškaitis, and T. Wilson, Proc. Natl. Acad. Sci. USA 99, 5788 (2002).
[CrossRef]

Appl. Phys. Lett. (1)

Y. Barad, H. Eisenberg, M. Horowitz, and Y. Silberberg, Appl. Phys. Lett. 70, 922 (1997).
[CrossRef]

J. Opt. Soc. Am. A (1)

Opt. Express (3)

Opt. Lett. (3)

Proc. Natl. Acad. Sci. USA (2)

M. Rueckel, J. Mack-Bucher, and W. Denk, Proc. Natl. Acad. Sci. USA 103, 17137 (2006).
[CrossRef]

M. J. Booth, M. A. A. Neil, R. Juškaitis, and T. Wilson, Proc. Natl. Acad. Sci. USA 99, 5788 (2002).
[CrossRef]

Supplementary Material (4)

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