Abstract

We demonstrate a technique for differential coherent anti-Stokes Raman scattering (CARS) microscopy employing linearly chirped femtosecond laser pulses. By replicating the exciting pump-Stokes pulse pairs to create a pulse train at twice the laser repetition rate, and controlling the instantaneous frequency difference of each pair by glass dispersion, we can adjust the Raman frequency probed by each pair in an intrinsically stable and cost-effective way. The resulting CARS intensities are detected by a single photomultiplier as sum and difference using phase-sensitive frequency filtering. We demonstrate imaging of polymer beads and living cells with suppressed nonresonant CARS background and improved chemical sensitivity.

© 2009 Optical Society of America

Full Article  |  PDF Article
OSA Recommended Articles
Chirped-probe-pulse femtosecond coherent anti-Stokes Raman scattering concentration measurements

Daniel R. Richardson, Robert P. Lucht, Waruna D. Kulatilaka, Sukesh Roy, and James R. Gord
J. Opt. Soc. Am. B 30(1) 188-196 (2013)

Single laser source for multimodal coherent anti-Stokes Raman scattering microscopy

Adrian F. Pegoraro, Aaron D. Slepkov, Andrew Ridsdale, John Paul Pezacki, and Albert Stolow
Appl. Opt. 49(25) F10-F17 (2010)

Elliptically polarized coherent anti-Stokes Raman scattering microscopy

Fake Lu, Wei Zheng, and Zhiwei Huang
Opt. Lett. 33(23) 2842-2844 (2008)

References

You do not have subscription access to this journal. Citation lists with outbound citation links are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription

Cited By

You do not have subscription access to this journal. Cited by links are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription

Figures (4)

You do not have subscription access to this journal. Figure files are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription

Metrics

You do not have subscription access to this journal. Article level metrics are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription