Abstract

We present multiphoton microendoscopy with a rotational probe and a 1μm fiber-based femtosecond laser. The rotational probe is based on a double-clad photonic crystal fiber, a gradient index lens, a microprism, and a rotational microelectronicmechanical system (MEMS) motor. The MEMS motor has a diameter of 2.2mm and can provide 360° full-view rotation. The fiber laser provides ultrashort pulses with a central wavelength at 1.034μm and a repetition rate of 50MHz. Second-harmonic-generation images of rat-tail tendon and fish scale are demonstrated with the rotational probe-based multiphoton system.

© 2009 Optical Society of America

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References

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  1. F. Helmchen, W. Denk, Nat. Med. 2, 932 (2005).
    [CrossRef]
  2. B. J. Tromberg, in Handbook of Biomedical Nonlinear Optical Microscopy, B.R.Masters and P.T. C.So, eds. (Oxford U. Press, 2008), pp. 707-715.
  3. M. Balu, T. Baldacchini, J. Carter, T. B. Krasieva, R. Zadoyan, and B. J. Tromberg, J. Biomed. Opt. 14, 010508-1 (2009).
    [CrossRef]
  4. J. C. Jung and M. J. Schnitzer, Opt. Lett. 28, 902 (2003).
    [CrossRef] [PubMed]
  5. B. A. Flusberg, E. D. Cocker, W. Piyawattanametha, J. C. Jung, E. L. M. Cheung, and M. J. Schnitzer, Nat. Med. 2, 941 (2005).
    [CrossRef]
  6. M. T. Myaing, J. Y. Ye, T. B. Norris, T. Thomas, J. R. Baker Jr., W. J. Wadsworth, G. Bouwmans, J. C. Knight, and P. St. J. Russell, Opt. Lett. 28, 1224 (2003).
    [CrossRef] [PubMed]
  7. W. Göbel, J. N. D. Kerr, A. L. Nimmerjahn, and F. Helmchen, Opt. Lett. 29, 2521 (2004).
    [CrossRef] [PubMed]
  8. L. Fu, X. Gan, and M. Gu, Opt. Express 13, 5528 (2005).
    [CrossRef] [PubMed]
  9. W. Jung, S. Tang, D. T. McCormic, T. Xie, Y. Ahn, J. Su, I. V. Tomov, T. B. Krasieva, B. J. Tromberg, and Z. Chen, Opt. Lett. 33, 1324 (2008).
    [CrossRef] [PubMed]
  10. M. T. Myaing, D. J. MacDonald, and X. Li, Opt. Lett. 31, 1076 (2006).
    [CrossRef] [PubMed]
  11. C. J. Engelbrecht1, R. S. Johnston, E. J. Seibel, and F. Helmchen, Opt. Express 16, 5556 (2008).
    [CrossRef]
  12. H. Lim, F. Ö. Ilday, and F. W. Wise, Opt. Lett. 28, 660 (2003).
    [CrossRef] [PubMed]
  13. J. Su, J. Zhang, L. Yu, and Z. Chen, Opt. Express 15, 10390 (2007).
    [CrossRef] [PubMed]

2009 (1)

M. Balu, T. Baldacchini, J. Carter, T. B. Krasieva, R. Zadoyan, and B. J. Tromberg, J. Biomed. Opt. 14, 010508-1 (2009).
[CrossRef]

2008 (2)

2007 (1)

2006 (1)

2005 (3)

B. A. Flusberg, E. D. Cocker, W. Piyawattanametha, J. C. Jung, E. L. M. Cheung, and M. J. Schnitzer, Nat. Med. 2, 941 (2005).
[CrossRef]

F. Helmchen, W. Denk, Nat. Med. 2, 932 (2005).
[CrossRef]

L. Fu, X. Gan, and M. Gu, Opt. Express 13, 5528 (2005).
[CrossRef] [PubMed]

2004 (1)

2003 (3)

Ahn, Y.

Baker, J. R.

Baldacchini, T.

M. Balu, T. Baldacchini, J. Carter, T. B. Krasieva, R. Zadoyan, and B. J. Tromberg, J. Biomed. Opt. 14, 010508-1 (2009).
[CrossRef]

Balu, M.

M. Balu, T. Baldacchini, J. Carter, T. B. Krasieva, R. Zadoyan, and B. J. Tromberg, J. Biomed. Opt. 14, 010508-1 (2009).
[CrossRef]

Bouwmans, G.

Carter, J.

M. Balu, T. Baldacchini, J. Carter, T. B. Krasieva, R. Zadoyan, and B. J. Tromberg, J. Biomed. Opt. 14, 010508-1 (2009).
[CrossRef]

Chen, Z.

Cheung, E. L. M.

B. A. Flusberg, E. D. Cocker, W. Piyawattanametha, J. C. Jung, E. L. M. Cheung, and M. J. Schnitzer, Nat. Med. 2, 941 (2005).
[CrossRef]

Cocker, E. D.

B. A. Flusberg, E. D. Cocker, W. Piyawattanametha, J. C. Jung, E. L. M. Cheung, and M. J. Schnitzer, Nat. Med. 2, 941 (2005).
[CrossRef]

Denk, W.

F. Helmchen, W. Denk, Nat. Med. 2, 932 (2005).
[CrossRef]

Engelbrecht1, C. J.

Flusberg, B. A.

B. A. Flusberg, E. D. Cocker, W. Piyawattanametha, J. C. Jung, E. L. M. Cheung, and M. J. Schnitzer, Nat. Med. 2, 941 (2005).
[CrossRef]

Fu, L.

Gan, X.

Göbel, W.

Gu, M.

Helmchen, F.

Ilday, F. Ö.

Johnston, R. S.

Jung, J. C.

B. A. Flusberg, E. D. Cocker, W. Piyawattanametha, J. C. Jung, E. L. M. Cheung, and M. J. Schnitzer, Nat. Med. 2, 941 (2005).
[CrossRef]

J. C. Jung and M. J. Schnitzer, Opt. Lett. 28, 902 (2003).
[CrossRef] [PubMed]

Jung, W.

Kerr, J. N. D.

Knight, J. C.

Krasieva, T. B.

M. Balu, T. Baldacchini, J. Carter, T. B. Krasieva, R. Zadoyan, and B. J. Tromberg, J. Biomed. Opt. 14, 010508-1 (2009).
[CrossRef]

W. Jung, S. Tang, D. T. McCormic, T. Xie, Y. Ahn, J. Su, I. V. Tomov, T. B. Krasieva, B. J. Tromberg, and Z. Chen, Opt. Lett. 33, 1324 (2008).
[CrossRef] [PubMed]

Li, X.

Lim, H.

MacDonald, D. J.

McCormic, D. T.

Myaing, M. T.

Nimmerjahn, A. L.

Norris, T. B.

Piyawattanametha, W.

B. A. Flusberg, E. D. Cocker, W. Piyawattanametha, J. C. Jung, E. L. M. Cheung, and M. J. Schnitzer, Nat. Med. 2, 941 (2005).
[CrossRef]

Russell, P. St. J.

Schnitzer, M. J.

B. A. Flusberg, E. D. Cocker, W. Piyawattanametha, J. C. Jung, E. L. M. Cheung, and M. J. Schnitzer, Nat. Med. 2, 941 (2005).
[CrossRef]

J. C. Jung and M. J. Schnitzer, Opt. Lett. 28, 902 (2003).
[CrossRef] [PubMed]

Seibel, E. J.

Su, J.

Tang, S.

Thomas, T.

Tomov, I. V.

Tromberg, B. J.

M. Balu, T. Baldacchini, J. Carter, T. B. Krasieva, R. Zadoyan, and B. J. Tromberg, J. Biomed. Opt. 14, 010508-1 (2009).
[CrossRef]

W. Jung, S. Tang, D. T. McCormic, T. Xie, Y. Ahn, J. Su, I. V. Tomov, T. B. Krasieva, B. J. Tromberg, and Z. Chen, Opt. Lett. 33, 1324 (2008).
[CrossRef] [PubMed]

B. J. Tromberg, in Handbook of Biomedical Nonlinear Optical Microscopy, B.R.Masters and P.T. C.So, eds. (Oxford U. Press, 2008), pp. 707-715.

Wadsworth, W. J.

Wise, F. W.

Xie, T.

Ye, J. Y.

Yu, L.

Zadoyan, R.

M. Balu, T. Baldacchini, J. Carter, T. B. Krasieva, R. Zadoyan, and B. J. Tromberg, J. Biomed. Opt. 14, 010508-1 (2009).
[CrossRef]

Zhang, J.

J. Biomed. Opt. (1)

M. Balu, T. Baldacchini, J. Carter, T. B. Krasieva, R. Zadoyan, and B. J. Tromberg, J. Biomed. Opt. 14, 010508-1 (2009).
[CrossRef]

Nat. Med. (2)

B. A. Flusberg, E. D. Cocker, W. Piyawattanametha, J. C. Jung, E. L. M. Cheung, and M. J. Schnitzer, Nat. Med. 2, 941 (2005).
[CrossRef]

F. Helmchen, W. Denk, Nat. Med. 2, 932 (2005).
[CrossRef]

Opt. Express (3)

Opt. Lett. (6)

Other (1)

B. J. Tromberg, in Handbook of Biomedical Nonlinear Optical Microscopy, B.R.Masters and P.T. C.So, eds. (Oxford U. Press, 2008), pp. 707-715.

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Figures (4)

Fig. 1
Fig. 1

Diagram of the multiphoton imaging system. A, fiber laser oscillator; B, fiber amplifier; C, grating pair compressor; D, the microprobe, data acquisition, and processing system. WDM, wavelength-division multiplexer; PCF, photonic crystal fiber; HWP, half-wave plate; PMT, photomultiplier tube; AMP, amplifier.

Fig. 2
Fig. 2

Double-logarithmic plot of the SHG intensity from KDP crystal power as a function of incident power.

Fig. 3
Fig. 3

Diagram of the microrotational probe. The probe is based on a DCPCF, a GRIN lens, a microprism, and a rotational MEMS motor. (b) Photograph of the microprism glued to a MEMS motor together with a United States dime coin.

Fig. 4
Fig. 4

SHG images of (a) a rat tail tendon and (b) a fish scale. Scale bar is 25 μ m .

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