Abstract

By detecting the transient four-wave mixing from gold nanoparticles in resonance with their surface plasmon, we demonstrate a multiphoton imaging modality suited for cell microscopy. Four-wave mixing is measured free from background using a three-beam excitation geometry and heterodyne detection. We achieve a spatial resolution of 140nm in-plane and 470nm in the axial direction, surpassing the one-photon diffraction limit. With this technique, high-contrast photostable imaging of Golgi structures is demonstrated in HepG2 cells labeled with gold nanoparticles of 10nm and 5nm diameter.

© 2009 Optical Society of America

Full Article  |  PDF Article
OSA Recommended Articles
Inorganic nanoparticles for optical bioimaging

Daniel Jaque, Cyrille Richard, Bruno Viana, Kohei Soga, Xiaogang Liu, and Jose García Solé
Adv. Opt. Photon. 8(1) 1-103 (2016)

Imaging the uptake of gold nanoshells in live cells using plasmon resonance enhanced four wave mixing microscopy

Natalie Garrett, Matt Whiteman, and Julian Moger
Opt. Express 19(18) 17563-17574 (2011)

Shot-noise limited localization of single 20 nm gold particles with nanometer spatial precision within microseconds

Ying-Hsiu Lin, Wei-Lin Chang, and Chia-Lung Hsieh
Opt. Express 22(8) 9159-9170 (2014)

References

You do not have subscription access to this journal. Citation lists with outbound citation links are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription

Cited By

You do not have subscription access to this journal. Cited by links are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription

Figures (3)

You do not have subscription access to this journal. Figure files are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription

Metrics

You do not have subscription access to this journal. Article level metrics are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription