Abstract

We have developed a laser-scanning optical-resolution photoacoustic microscopy method that can potentially fuse with existing optical microscopic imaging modalities. To acquire an image, the ultrasonic transducer is kept stationary during data acquisition, and only the laser light is raster scanned by an xy galvanometer scanner. A lateral resolution of 7.8μm and a circular field of view with a diameter of 6mm were achieved in an optically clear medium. Using a laser system working at a pulse repetition rate of 1024Hz, the data acquisition time for an image consisting of 256×256  pixels was less than 2 min.

© 2009 Optical Society of America

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2008

2007

Laser Institute of America, American National Standard for Safe Use of Lasers ANSI Z136.1-2007 (American National Standards Institute, Inc., 2007).

H. F. Zhang, K. Maslov, and L. V. Wang, Nat. Protocols 4, 797 (2007).
[CrossRef]

2006

H. F. Zhang, K. Maslov, G. Stoica, and L. V. Wang, Nat. Biotechnol. 24, 848 (2006).
[CrossRef] [PubMed]

S. Tang, T. B. Krasieva, Z. Chen, and B. J. Tromberg, J. Biomed. Opt. 11, 020502 (2006).
[CrossRef] [PubMed]

2005

D. A. Sipkins, X. Wei, J. W. Wu, J. M. Runnels, D. Côté, T. K. Means, A. D. Luster, D. T. Scadden, and C. P. Lin, Nature 435, 969 (2005).
[CrossRef] [PubMed]

2003

A. A. Oraevsky and A. A. Karabutov, in Biomedical Photonics Handbook, T.Vo-Dinh, ed. (CRC Press, 2003), Vol. PM125, Chap. 34.

X. Wang, Y. Pang, G. Ku, X. Xie, G. Stoica, and L. V. Wang, Nat. Biotechnol. 25, 114 (2003).

Bosschaart, N.

Chen, Z.

S. Tang, T. B. Krasieva, Z. Chen, and B. J. Tromberg, J. Biomed. Opt. 11, 020502 (2006).
[CrossRef] [PubMed]

Côté, D.

D. A. Sipkins, X. Wei, J. W. Wu, J. M. Runnels, D. Côté, T. K. Means, A. D. Luster, D. T. Scadden, and C. P. Lin, Nature 435, 969 (2005).
[CrossRef] [PubMed]

Galanzha, E. I.

Hu, S.

Karabutov, A. A.

A. A. Oraevsky and A. A. Karabutov, in Biomedical Photonics Handbook, T.Vo-Dinh, ed. (CRC Press, 2003), Vol. PM125, Chap. 34.

Keller, M. D.

Khlebtsov, N. G.

Krasieva, T. B.

S. Tang, T. B. Krasieva, Z. Chen, and B. J. Tromberg, J. Biomed. Opt. 11, 020502 (2006).
[CrossRef] [PubMed]

Ku, G.

X. Wang, Y. Pang, G. Ku, X. Xie, G. Stoica, and L. V. Wang, Nat. Biotechnol. 25, 114 (2003).

Lin, C. P.

D. A. Sipkins, X. Wei, J. W. Wu, J. M. Runnels, D. Côté, T. K. Means, A. D. Luster, D. T. Scadden, and C. P. Lin, Nature 435, 969 (2005).
[CrossRef] [PubMed]

Luster, A. D.

D. A. Sipkins, X. Wei, J. W. Wu, J. M. Runnels, D. Côté, T. K. Means, A. D. Luster, D. T. Scadden, and C. P. Lin, Nature 435, 969 (2005).
[CrossRef] [PubMed]

Mahadevan-Jansen, A.

Maslov, K.

K. Maslov, H. F. Zhang, S. Hu, and L. V. Wang, Opt. Lett. 33, 929 (2008).
[CrossRef] [PubMed]

H. F. Zhang, K. Maslov, and L. V. Wang, Nat. Protocols 4, 797 (2007).
[CrossRef]

H. F. Zhang, K. Maslov, G. Stoica, and L. V. Wang, Nat. Biotechnol. 24, 848 (2006).
[CrossRef] [PubMed]

Means, T. K.

D. A. Sipkins, X. Wei, J. W. Wu, J. M. Runnels, D. Côté, T. K. Means, A. D. Luster, D. T. Scadden, and C. P. Lin, Nature 435, 969 (2005).
[CrossRef] [PubMed]

Oraevsky, A. A.

A. A. Oraevsky and A. A. Karabutov, in Biomedical Photonics Handbook, T.Vo-Dinh, ed. (CRC Press, 2003), Vol. PM125, Chap. 34.

Pang, Y.

X. Wang, Y. Pang, G. Ku, X. Xie, G. Stoica, and L. V. Wang, Nat. Biotechnol. 25, 114 (2003).

Patil, C. A.

Runnels, J. M.

D. A. Sipkins, X. Wei, J. W. Wu, J. M. Runnels, D. Côté, T. K. Means, A. D. Luster, D. T. Scadden, and C. P. Lin, Nature 435, 969 (2005).
[CrossRef] [PubMed]

Scadden, D. T.

D. A. Sipkins, X. Wei, J. W. Wu, J. M. Runnels, D. Côté, T. K. Means, A. D. Luster, D. T. Scadden, and C. P. Lin, Nature 435, 969 (2005).
[CrossRef] [PubMed]

Shashkov, E. V.

Sipkins, D. A.

D. A. Sipkins, X. Wei, J. W. Wu, J. M. Runnels, D. Côté, T. K. Means, A. D. Luster, D. T. Scadden, and C. P. Lin, Nature 435, 969 (2005).
[CrossRef] [PubMed]

Stoica, G.

H. F. Zhang, K. Maslov, G. Stoica, and L. V. Wang, Nat. Biotechnol. 24, 848 (2006).
[CrossRef] [PubMed]

X. Wang, Y. Pang, G. Ku, X. Xie, G. Stoica, and L. V. Wang, Nat. Biotechnol. 25, 114 (2003).

Tang, S.

S. Tang, T. B. Krasieva, Z. Chen, and B. J. Tromberg, J. Biomed. Opt. 11, 020502 (2006).
[CrossRef] [PubMed]

Tromberg, B. J.

S. Tang, T. B. Krasieva, Z. Chen, and B. J. Tromberg, J. Biomed. Opt. 11, 020502 (2006).
[CrossRef] [PubMed]

Tuchin, V. V.

van Leeuwen, T. G.

Wang, L. V.

K. Maslov, H. F. Zhang, S. Hu, and L. V. Wang, Opt. Lett. 33, 929 (2008).
[CrossRef] [PubMed]

H. F. Zhang, K. Maslov, and L. V. Wang, Nat. Protocols 4, 797 (2007).
[CrossRef]

H. F. Zhang, K. Maslov, G. Stoica, and L. V. Wang, Nat. Biotechnol. 24, 848 (2006).
[CrossRef] [PubMed]

X. Wang, Y. Pang, G. Ku, X. Xie, G. Stoica, and L. V. Wang, Nat. Biotechnol. 25, 114 (2003).

Wang, X.

X. Wang, Y. Pang, G. Ku, X. Xie, G. Stoica, and L. V. Wang, Nat. Biotechnol. 25, 114 (2003).

Wei, X.

D. A. Sipkins, X. Wei, J. W. Wu, J. M. Runnels, D. Côté, T. K. Means, A. D. Luster, D. T. Scadden, and C. P. Lin, Nature 435, 969 (2005).
[CrossRef] [PubMed]

Wu, J. W.

D. A. Sipkins, X. Wei, J. W. Wu, J. M. Runnels, D. Côté, T. K. Means, A. D. Luster, D. T. Scadden, and C. P. Lin, Nature 435, 969 (2005).
[CrossRef] [PubMed]

Xie, X.

X. Wang, Y. Pang, G. Ku, X. Xie, G. Stoica, and L. V. Wang, Nat. Biotechnol. 25, 114 (2003).

Zhang, H. F.

K. Maslov, H. F. Zhang, S. Hu, and L. V. Wang, Opt. Lett. 33, 929 (2008).
[CrossRef] [PubMed]

H. F. Zhang, K. Maslov, and L. V. Wang, Nat. Protocols 4, 797 (2007).
[CrossRef]

H. F. Zhang, K. Maslov, G. Stoica, and L. V. Wang, Nat. Biotechnol. 24, 848 (2006).
[CrossRef] [PubMed]

Zharov, V. P.

J. Biomed. Opt.

S. Tang, T. B. Krasieva, Z. Chen, and B. J. Tromberg, J. Biomed. Opt. 11, 020502 (2006).
[CrossRef] [PubMed]

Nat. Biotechnol.

H. F. Zhang, K. Maslov, G. Stoica, and L. V. Wang, Nat. Biotechnol. 24, 848 (2006).
[CrossRef] [PubMed]

X. Wang, Y. Pang, G. Ku, X. Xie, G. Stoica, and L. V. Wang, Nat. Biotechnol. 25, 114 (2003).

Nat. Protocols

H. F. Zhang, K. Maslov, and L. V. Wang, Nat. Protocols 4, 797 (2007).
[CrossRef]

Nature

D. A. Sipkins, X. Wei, J. W. Wu, J. M. Runnels, D. Côté, T. K. Means, A. D. Luster, D. T. Scadden, and C. P. Lin, Nature 435, 969 (2005).
[CrossRef] [PubMed]

Opt. Lett.

Other

Laser Institute of America, American National Standard for Safe Use of Lasers ANSI Z136.1-2007 (American National Standards Institute, Inc., 2007).

A. A. Oraevsky and A. A. Karabutov, in Biomedical Photonics Handbook, T.Vo-Dinh, ed. (CRC Press, 2003), Vol. PM125, Chap. 34.

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Figures (3)

Fig. 1
Fig. 1

Schematic of the LSOR-PAM experimental setup.

Fig. 2
Fig. 2

Quantification of the FOV and the spatial resolutions. (a) LSOR-PAM image of the printed mesh grid. (b) LSOR-PAM image of the USAF resolution target. (c) PA values at the cross sections highlighted by a-a and b-b in (b). (d) Estimate of axial resolution from a typical A-line acquired from blood vessel. sd, shift distance.

Fig. 3
Fig. 3

LSOR-PAM image of vasculature in a Swiss Webster mouse ear in vivo. (a) Photograph of the ear. (b) LSOR-PAM image of vasculature within the marked region in (a). Two pairs of parallel veins and arteries are highlighted by 1 and 2.

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