Abstract

We have developed a laser-scanning optical-resolution photoacoustic microscopy method that can potentially fuse with existing optical microscopic imaging modalities. To acquire an image, the ultrasonic transducer is kept stationary during data acquisition, and only the laser light is raster scanned by an x-y galvanometer scanner. A lateral resolution of 7.8 μm and a circular field of view with a diameter of 6 mm were achieved in an optically clear medium. Using a laser system working at a pulse repetition rate of 1024 Hz, the data acquisition time for an image consisting of 256×256 pixels was less than 2 min.

© 2009 Optical Society of America

PDF Article

References

  • View by:
  • |
  • |
  • |

  1. D. A. Sipkins, X. Wei, J. W. Wu, J. M. Runnels, D. Côté, T. K. Means, A. D. Luster, D. T. Scadden, and C. P. Lin, Nature 435, 969 (2005).
    [CrossRef]
  2. C. A. Patil, N. Bosschaart, M. D. Keller, T. G. van Leeuwen, and A. Mahadevan-Jansen, Opt. Lett. 33, 1135 (2008).
    [CrossRef]
  3. S. Tang, T. B. Krasieva, Z. Chen, and B. J. Tromberg, J. Biomed. Opt. 11, 020502 (2006).
    [CrossRef]
  4. A. A. Oraevsky and A. A. Karabutov, in Biomedical Photonics Handbook, T.Vo-Dinh, ed. (CRC Press, 2003), Vol. PM125, Chap. 34.
  5. X. Wang, Y. Pang, G. Ku, X. Xie, G. Stoica, and L. V. Wang, Nat. Biotechnol. 25, 114 (2003).
  6. H. F. Zhang, K. Maslov, and L. V. Wang, Nat. Protocols 4, 797 (2007).
    [CrossRef]
  7. H. F. Zhang, K. Maslov, G. Stoica, and L. V. Wang, Nat. Biotechnol. 24, 848 (2006).
    [CrossRef]
  8. K. Maslov, H. F. Zhang, S. Hu, and L. V. Wang, Opt. Lett. 33, 929 (2008).
    [CrossRef]
  9. V. P. Zharov, E. I. Galanzha, E. V. Shashkov, N. G. Khlebtsov, and V. V. Tuchin, Opt. Lett. 31, 3623 (2008).
    [CrossRef]
  10. Laser Institute of America, American National Standard for Safe Use of Lasers ANSI Z136.1-2007 (American National Standards Institute, Inc., 2007).

2008 (3)

2007 (2)

Laser Institute of America, American National Standard for Safe Use of Lasers ANSI Z136.1-2007 (American National Standards Institute, Inc., 2007).

H. F. Zhang, K. Maslov, and L. V. Wang, Nat. Protocols 4, 797 (2007).
[CrossRef]

2006 (2)

H. F. Zhang, K. Maslov, G. Stoica, and L. V. Wang, Nat. Biotechnol. 24, 848 (2006).
[CrossRef]

S. Tang, T. B. Krasieva, Z. Chen, and B. J. Tromberg, J. Biomed. Opt. 11, 020502 (2006).
[CrossRef]

2005 (1)

D. A. Sipkins, X. Wei, J. W. Wu, J. M. Runnels, D. Côté, T. K. Means, A. D. Luster, D. T. Scadden, and C. P. Lin, Nature 435, 969 (2005).
[CrossRef]

2003 (2)

A. A. Oraevsky and A. A. Karabutov, in Biomedical Photonics Handbook, T.Vo-Dinh, ed. (CRC Press, 2003), Vol. PM125, Chap. 34.

X. Wang, Y. Pang, G. Ku, X. Xie, G. Stoica, and L. V. Wang, Nat. Biotechnol. 25, 114 (2003).

Bosschaart, N.

Chen, Z.

S. Tang, T. B. Krasieva, Z. Chen, and B. J. Tromberg, J. Biomed. Opt. 11, 020502 (2006).
[CrossRef]

Côté, D.

D. A. Sipkins, X. Wei, J. W. Wu, J. M. Runnels, D. Côté, T. K. Means, A. D. Luster, D. T. Scadden, and C. P. Lin, Nature 435, 969 (2005).
[CrossRef]

Galanzha, E. I.

Hu, S.

Karabutov, A. A.

A. A. Oraevsky and A. A. Karabutov, in Biomedical Photonics Handbook, T.Vo-Dinh, ed. (CRC Press, 2003), Vol. PM125, Chap. 34.

Keller, M. D.

Khlebtsov, N. G.

Krasieva, T. B.

S. Tang, T. B. Krasieva, Z. Chen, and B. J. Tromberg, J. Biomed. Opt. 11, 020502 (2006).
[CrossRef]

Ku, G.

X. Wang, Y. Pang, G. Ku, X. Xie, G. Stoica, and L. V. Wang, Nat. Biotechnol. 25, 114 (2003).

Lin, C. P.

D. A. Sipkins, X. Wei, J. W. Wu, J. M. Runnels, D. Côté, T. K. Means, A. D. Luster, D. T. Scadden, and C. P. Lin, Nature 435, 969 (2005).
[CrossRef]

Luster, A. D.

D. A. Sipkins, X. Wei, J. W. Wu, J. M. Runnels, D. Côté, T. K. Means, A. D. Luster, D. T. Scadden, and C. P. Lin, Nature 435, 969 (2005).
[CrossRef]

Mahadevan-Jansen, A.

Maslov, K.

K. Maslov, H. F. Zhang, S. Hu, and L. V. Wang, Opt. Lett. 33, 929 (2008).
[CrossRef]

H. F. Zhang, K. Maslov, and L. V. Wang, Nat. Protocols 4, 797 (2007).
[CrossRef]

H. F. Zhang, K. Maslov, G. Stoica, and L. V. Wang, Nat. Biotechnol. 24, 848 (2006).
[CrossRef]

Means, T. K.

D. A. Sipkins, X. Wei, J. W. Wu, J. M. Runnels, D. Côté, T. K. Means, A. D. Luster, D. T. Scadden, and C. P. Lin, Nature 435, 969 (2005).
[CrossRef]

Oraevsky, A. A.

A. A. Oraevsky and A. A. Karabutov, in Biomedical Photonics Handbook, T.Vo-Dinh, ed. (CRC Press, 2003), Vol. PM125, Chap. 34.

Pang, Y.

X. Wang, Y. Pang, G. Ku, X. Xie, G. Stoica, and L. V. Wang, Nat. Biotechnol. 25, 114 (2003).

Patil, C. A.

Runnels, J. M.

D. A. Sipkins, X. Wei, J. W. Wu, J. M. Runnels, D. Côté, T. K. Means, A. D. Luster, D. T. Scadden, and C. P. Lin, Nature 435, 969 (2005).
[CrossRef]

Scadden, D. T.

D. A. Sipkins, X. Wei, J. W. Wu, J. M. Runnels, D. Côté, T. K. Means, A. D. Luster, D. T. Scadden, and C. P. Lin, Nature 435, 969 (2005).
[CrossRef]

Shashkov, E. V.

Sipkins, D. A.

D. A. Sipkins, X. Wei, J. W. Wu, J. M. Runnels, D. Côté, T. K. Means, A. D. Luster, D. T. Scadden, and C. P. Lin, Nature 435, 969 (2005).
[CrossRef]

Stoica, G.

H. F. Zhang, K. Maslov, G. Stoica, and L. V. Wang, Nat. Biotechnol. 24, 848 (2006).
[CrossRef]

X. Wang, Y. Pang, G. Ku, X. Xie, G. Stoica, and L. V. Wang, Nat. Biotechnol. 25, 114 (2003).

Tang, S.

S. Tang, T. B. Krasieva, Z. Chen, and B. J. Tromberg, J. Biomed. Opt. 11, 020502 (2006).
[CrossRef]

Tromberg, B. J.

S. Tang, T. B. Krasieva, Z. Chen, and B. J. Tromberg, J. Biomed. Opt. 11, 020502 (2006).
[CrossRef]

Tuchin, V. V.

van Leeuwen, T. G.

Wang, L. V.

K. Maslov, H. F. Zhang, S. Hu, and L. V. Wang, Opt. Lett. 33, 929 (2008).
[CrossRef]

H. F. Zhang, K. Maslov, and L. V. Wang, Nat. Protocols 4, 797 (2007).
[CrossRef]

H. F. Zhang, K. Maslov, G. Stoica, and L. V. Wang, Nat. Biotechnol. 24, 848 (2006).
[CrossRef]

X. Wang, Y. Pang, G. Ku, X. Xie, G. Stoica, and L. V. Wang, Nat. Biotechnol. 25, 114 (2003).

Wang, X.

X. Wang, Y. Pang, G. Ku, X. Xie, G. Stoica, and L. V. Wang, Nat. Biotechnol. 25, 114 (2003).

Wei, X.

D. A. Sipkins, X. Wei, J. W. Wu, J. M. Runnels, D. Côté, T. K. Means, A. D. Luster, D. T. Scadden, and C. P. Lin, Nature 435, 969 (2005).
[CrossRef]

Wu, J. W.

D. A. Sipkins, X. Wei, J. W. Wu, J. M. Runnels, D. Côté, T. K. Means, A. D. Luster, D. T. Scadden, and C. P. Lin, Nature 435, 969 (2005).
[CrossRef]

Xie, X.

X. Wang, Y. Pang, G. Ku, X. Xie, G. Stoica, and L. V. Wang, Nat. Biotechnol. 25, 114 (2003).

Zhang, H. F.

K. Maslov, H. F. Zhang, S. Hu, and L. V. Wang, Opt. Lett. 33, 929 (2008).
[CrossRef]

H. F. Zhang, K. Maslov, and L. V. Wang, Nat. Protocols 4, 797 (2007).
[CrossRef]

H. F. Zhang, K. Maslov, G. Stoica, and L. V. Wang, Nat. Biotechnol. 24, 848 (2006).
[CrossRef]

Zharov, V. P.

J. Biomed. Opt. (1)

S. Tang, T. B. Krasieva, Z. Chen, and B. J. Tromberg, J. Biomed. Opt. 11, 020502 (2006).
[CrossRef]

Nat. Biotechnol. (2)

X. Wang, Y. Pang, G. Ku, X. Xie, G. Stoica, and L. V. Wang, Nat. Biotechnol. 25, 114 (2003).

H. F. Zhang, K. Maslov, G. Stoica, and L. V. Wang, Nat. Biotechnol. 24, 848 (2006).
[CrossRef]

Nat. Protocols (1)

H. F. Zhang, K. Maslov, and L. V. Wang, Nat. Protocols 4, 797 (2007).
[CrossRef]

Nature (1)

D. A. Sipkins, X. Wei, J. W. Wu, J. M. Runnels, D. Côté, T. K. Means, A. D. Luster, D. T. Scadden, and C. P. Lin, Nature 435, 969 (2005).
[CrossRef]

Opt. Lett. (3)

Other (2)

Laser Institute of America, American National Standard for Safe Use of Lasers ANSI Z136.1-2007 (American National Standards Institute, Inc., 2007).

A. A. Oraevsky and A. A. Karabutov, in Biomedical Photonics Handbook, T.Vo-Dinh, ed. (CRC Press, 2003), Vol. PM125, Chap. 34.

Cited By

OSA participates in CrossRef's Cited-By Linking service. Citing articles from OSA journals and other participating publishers are listed here.

Alert me when this article is cited.


Metrics