Abstract

The in vivo flow cytometer enables the real-time detection and quantification of fluorescent cells circulating within a live animal without the need for incisions or extraction of blood. It has been used in demonstrating flow velocity disparities in biological flows, and in the investigation of the circulation kinetics of various types of cells. However, a shortcoming of this in vivo flow cytometer is that it provides only one excitation slit at one wavelength, resulting in several performance limitations. Therefore, a second in vivo flow cytometer that provides two different laser wavelengths, 473 and 633nm, and one or two excitation slits has been designed and built. Thus far, the two-color system has been used to acquire circulation kinetics data of two different cell populations each labeled with a different marker, one cell population labeled with two different markers, and one cell population expressing the green-fluorescent protein gene. In addition, accurate arterial red blood cell velocities within a mouse have been determined using the cytometer.

© 2007 Optical Society of America

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References

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  1. J. P. Novak, 'Development of the in vivo flow cytometer,' Ph.D. thesis (Massachusetts Institute of Technology, 2004).
  2. J. Novak, I. Georgakoudi, X. Wei, A. Prossin, and C. P. Lin, Opt. Lett. 29, 77 (2004).
    [CrossRef] [PubMed]
  3. I. Georgakoudi, N. Solban, J. Novak, W. Rice, X. Wei, T. Hasan, and C. P. Lin, Cancer Res. 64, 5044 (2004).
    [CrossRef] [PubMed]
  4. Z. Chen, T. Milner, S. Srinivas, A. Malekafzali, J. C. Martin, and J. S. Nelson, Opt. Lett. 22, 1119 (1997).
    [CrossRef] [PubMed]

2004 (2)

J. Novak, I. Georgakoudi, X. Wei, A. Prossin, and C. P. Lin, Opt. Lett. 29, 77 (2004).
[CrossRef] [PubMed]

I. Georgakoudi, N. Solban, J. Novak, W. Rice, X. Wei, T. Hasan, and C. P. Lin, Cancer Res. 64, 5044 (2004).
[CrossRef] [PubMed]

1997 (1)

Cancer Res. (1)

I. Georgakoudi, N. Solban, J. Novak, W. Rice, X. Wei, T. Hasan, and C. P. Lin, Cancer Res. 64, 5044 (2004).
[CrossRef] [PubMed]

Opt. Lett. (2)

Other (1)

J. P. Novak, 'Development of the in vivo flow cytometer,' Ph.D. thesis (Massachusetts Institute of Technology, 2004).

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Figures (5)

Fig. 1
Fig. 1

Schematic of the two-color, double-slit in vivo flow cytometer [1].

Fig. 2
Fig. 2

Detection of leukocyte cells expressing the EGFP gene circulating in the blood stream of a C57LB6 mouse [1].

Fig. 3
Fig. 3

Detection of human red blood cells labeled with DiD circulating in the blood stream of a BALB/c mouse [1].

Fig. 4
Fig. 4

Detection of T-cells of a BALB/c mouse, colabeled with Cell Tracker and DiD by the 473 nm channel (A) and the 633 nm channel (B), respectively [1]. Cell tracker is the fluorescence source for the 473 nm channel and DiD is the fluorescence source for the 633 nm .

Fig. 5
Fig. 5

Detection of human red blood cells in a BALB/c mouse labeled with DiD using two excitation slits at 633 nm wavelength [1].

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