Abstract

We demonstrate that both oxyhemoglobin and deoxyhemoglobin have sequential two-color, two-photon absorption properties that can serve as endogenous contrasts in microvasculature imaging. Using a sensitive modulation transfer technique, we are able to image hemoglobin in red blood cells with micrometer resolution, both in vitro and in vivo. We show that excellent contrast from hemoglobin without any labeling can be obtained in tissue.

© 2007 Optical Society of America

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2007

D. Fu, T. Ye, T. E. Matthews, G. Yurtsever, L. Hong, J. D. Simon, and W. S. Warren, Proc. SPIE 6424, 642402 (2007).

G. O. Clay, C. B. Schaffer, and D. Kleinfeld, J. Chem. Phys. 126, 025102 (2007).
[CrossRef]

2006

V. P. Zharov, E. I. Galanzha, Y. Menyaev, and V. V. Tuchin, J. Biomed. Opt. 11, 054034 (2006).
[CrossRef]

A. R. Hilde, B. Mischa, M. V. Erik, B. S. Chris, and M. Michiel, J. Biomed. Opt. 11, 050502 (2006).
[CrossRef]

T. Ye, G. Yurtsever, M. Fischer, J. D. Simon, and W. S. Warren, Proc. SPIE 6089, 60891X (2006).

G. O. Clay, A. C. Millard, C. B. Schaffer, J. Aus-Der-Au, P. S. Tsai, J. A. Squier, and D. Kleinfeld, J. Opt. Soc. Am. B 23, 932 (2006).
[CrossRef]

2005

J. C. Miller, H. H. Pien, D. Sahani, A. G. Sorensen, and J. H. Thrall, J. Natl. Cancer Inst. 97, 172 (2005).

B. S. Sorg, B. J. Moeller, O. Donovan, Y. T. Cao, and M. W. Dewhirst, J. Biomed. Opt. 10, 044004 (2005).
[CrossRef]

M. C. Fischer, T. Ye, G. Yurtsever, A. Miller, M. Ciocca, W. Wagner, and W. S. Warren, Opt. Lett. 30, 1551 (2005).

B. A. Flusberg, J. C. Lung, E. D. Cocker, E. P. Anderson, and M. J. Schnitzer, Opt. Lett. 30, 2272 (2005).
[CrossRef]

2004

B. W. Pogue, S. D. Jiang, H. Dehghani, C. Kogel, S. Soho, S. Srinivasan, X. M. Song, T. D. Tosteson, S. P. Poplack, and K. D. Paulsen, J. Biomed. Opt. 9, 541 (2004).
[CrossRef]

2003

P. C. Lin, J. Cell. Biochem. 90, 484 (2003).

D. M. McDonald and P. L. Choyke, Nat. Med. 9, 713 (2003).

A. K. Dunn, A. Devor, H. Bolay, M. L. Andermann, M. A. Moskowitz, A. M. Dale, and D. A. Boas, Opt. Lett. 28, 28 (2003).

2002

2001

F. Helmchen, M. S. Fee, D. W. Tank, and W. Denk, Neuron 31, 903 (2001).
[CrossRef]

1999

1998

P. T. C. So, H. Kim, and I. E. Kochevar, Opt. Express 3, 339 (1998).

D. Kleinfeld, P. P. Mitra, F. Helmchen, and W. Denk, Proc. Natl. Acad. Sci. U.S.A. 95, 15741 (1998).
[CrossRef]

1986

A. Grinvald, E. Lieke, R. D. Frostig, C. D. Gilbert, and T. N. Wiesel, Nature 324, 361 (1986).
[CrossRef]

Appl. Opt.

J. Biomed. Opt.

V. P. Zharov, E. I. Galanzha, Y. Menyaev, and V. V. Tuchin, J. Biomed. Opt. 11, 054034 (2006).
[CrossRef]

B. S. Sorg, B. J. Moeller, O. Donovan, Y. T. Cao, and M. W. Dewhirst, J. Biomed. Opt. 10, 044004 (2005).
[CrossRef]

B. W. Pogue, S. D. Jiang, H. Dehghani, C. Kogel, S. Soho, S. Srinivasan, X. M. Song, T. D. Tosteson, S. P. Poplack, and K. D. Paulsen, J. Biomed. Opt. 9, 541 (2004).
[CrossRef]

A. R. Hilde, B. Mischa, M. V. Erik, B. S. Chris, and M. Michiel, J. Biomed. Opt. 11, 050502 (2006).
[CrossRef]

J. Cell. Biochem.

P. C. Lin, J. Cell. Biochem. 90, 484 (2003).

J. Chem. Phys.

G. O. Clay, C. B. Schaffer, and D. Kleinfeld, J. Chem. Phys. 126, 025102 (2007).
[CrossRef]

J. Natl. Cancer Inst.

J. C. Miller, H. H. Pien, D. Sahani, A. G. Sorensen, and J. H. Thrall, J. Natl. Cancer Inst. 97, 172 (2005).

J. Opt. Soc. Am. B

Nat. Med.

D. M. McDonald and P. L. Choyke, Nat. Med. 9, 713 (2003).

Nature

A. Grinvald, E. Lieke, R. D. Frostig, C. D. Gilbert, and T. N. Wiesel, Nature 324, 361 (1986).
[CrossRef]

Neuron

F. Helmchen, M. S. Fee, D. W. Tank, and W. Denk, Neuron 31, 903 (2001).
[CrossRef]

Opt. Express

Opt. Lett.

Proc. Natl. Acad. Sci. U.S.A.

D. Kleinfeld, P. P. Mitra, F. Helmchen, and W. Denk, Proc. Natl. Acad. Sci. U.S.A. 95, 15741 (1998).
[CrossRef]

Proc. SPIE

T. Ye, G. Yurtsever, M. Fischer, J. D. Simon, and W. S. Warren, Proc. SPIE 6089, 60891X (2006).

D. Fu, T. Ye, T. E. Matthews, G. Yurtsever, L. Hong, J. D. Simon, and W. S. Warren, Proc. SPIE 6424, 642402 (2007).

Other

G. Yurtsever, T. Ye, K. Weaver, and W. Warren, presented at the Biomedical Optics Topical Meeting, Fort Lauderdale, Fla., March 19-22, 2006.

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