Abstract

Angle-resolved light scattering spectroscopy of biological cells is investigated in the visible wavelength range. A unified Mie and fractal model is shown to provide an accurate global agreement with light scattering spectra from 1.1° to 165° scattering angles. It is found that light scattering in forward directions (<8°) is dominated by Mie scattering by the bare cell and nucleus, whereas light scattering at large angles (>20°) is determined by fractal scattering by subcellular structures. The findings are consistent with the results of experimental investigation of the contributions of different cellular components to light scattering by cells.

© 2007 Optical Society of America

Full Article  |  PDF Article
Related Articles
Light scattering from cells: finite-difference time-domain simulations and goniometric measurements

Rebekah Drezek, Andrew Dunn, and Rebekah Richards-Kortum
Appl. Opt. 38(16) 3651-3661 (1999)

Mechanisms of light scattering from biological cells relevant to noninvasive optical-tissue diagnostics

Judith R. Mourant, James P. Freyer, Andreas H. Hielscher, Angelia A. Eick, Dan Shen, and Tamara M. Johnson
Appl. Opt. 37(16) 3586-3593 (1998)

Reflectance spectroscopy with polarized light: is it sensitive to cellular and nuclear morphology

Konstantin Sokolov, Rebekah Drezek, Kirk Gossage, and Rebecca Richards-Kortum
Opt. Express 5(13) 302-317 (1999)

References

You do not have subscription access to this journal. Citation lists with outbound citation links are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription

Cited By

You do not have subscription access to this journal. Cited by links are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription

Figures (1)

You do not have subscription access to this journal. Figure files are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription

Tables (1)

You do not have subscription access to this journal. Article tables are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription

Equations (2)

You do not have subscription access to this journal. Equations are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription

Metrics

You do not have subscription access to this journal. Article level metrics are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription