Abstract

Angle-resolved light scattering spectroscopy of biological cells is investigated in the visible wavelength range. A unified Mie and fractal model is shown to provide an accurate global agreement with light scattering spectra from 1.1° to 165° scattering angles. It is found that light scattering in forward directions (<8°) is dominated by Mie scattering by the bare cell and nucleus, whereas light scattering at large angles (>20°) is determined by fractal scattering by subcellular structures. The findings are consistent with the results of experimental investigation of the contributions of different cellular components to light scattering by cells.

© 2007 Optical Society of America

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    [CrossRef] [PubMed]
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2007

M. Xu, T. T. Wu, and J. Y. Qu, Proc. SPIE 6446, 64460Y (2007).
[CrossRef]

T. T. Wu and J. Y. Qu, Appl. Opt. 46, 4834 (2007).
[CrossRef] [PubMed]

2006

R. S. Brock, X. H. Hu, D. A. Weidner, J. R. Mourant, and J. Q. Lu, J. Quant. Spectrosc. Radiat. Transf. 102, 25 (2006).
[CrossRef]

2005

X. Li, A. Taflove, and V. Backman, IEEE J. Sel. Top. Quantum Electron. 11, 759 (2005).
[CrossRef]

M. Xu and R. R. Alfano, Opt. Lett. 30, 3051 (2005).
[CrossRef] [PubMed]

2003

Z. Chen, A. Taflove, and V. Backman, Opt. Lett. 28, 765 (2003).
[CrossRef] [PubMed]

R. Lambert, J. F. Rey, and R. Sankaranarayanan, Endoscopy 35, 437 (2003).
[CrossRef] [PubMed]

2002

A. Wax, C. Yang, V. Backman, K. Badizadegan, C. W. Boone, R. R. Dasari, and M. S. Feld, Biophys. J. 82, 2256 (2002).
[CrossRef] [PubMed]

J. R. Mourant, T. M. Johnson, S. Carpenter, A. Guerra, T. Aida, and J. P. Freyer, J. Biomed. Opt. 7, 378 (2002).
[CrossRef] [PubMed]

2001

1999

1998

L. T. Perelman, V. Backman, M. Wallace, G. Zonios, R. Manoharan, A. Nusrat, S. Shields, M. Seiler, C. Lima, T. Hamano, I. Itzkan, J. Van Dam, J. M. Crawford, and M. S. Feld, Phys. Rev. Lett. 80, 627 (1998).
[CrossRef]

1980

Aida, T.

J. R. Mourant, T. M. Johnson, S. Carpenter, A. Guerra, T. Aida, and J. P. Freyer, J. Biomed. Opt. 7, 378 (2002).
[CrossRef] [PubMed]

Alfano, R. R.

Anderson, M. C.

M. C. Anderson, J. A. Jordan, A. R. Morse, and F. Sharp, A Text and Atlas of Integrated Colposcopy: for Colposcopists, Histopathologists and Cytologists (Chapman & Hall Medical, 1992).

Backman, V.

X. Li, A. Taflove, and V. Backman, IEEE J. Sel. Top. Quantum Electron. 11, 759 (2005).
[CrossRef]

Z. Chen, A. Taflove, and V. Backman, Opt. Lett. 28, 765 (2003).
[CrossRef] [PubMed]

A. Wax, C. Yang, V. Backman, K. Badizadegan, C. W. Boone, R. R. Dasari, and M. S. Feld, Biophys. J. 82, 2256 (2002).
[CrossRef] [PubMed]

L. T. Perelman, V. Backman, M. Wallace, G. Zonios, R. Manoharan, A. Nusrat, S. Shields, M. Seiler, C. Lima, T. Hamano, I. Itzkan, J. Van Dam, J. M. Crawford, and M. S. Feld, Phys. Rev. Lett. 80, 627 (1998).
[CrossRef]

Badizadegan, K.

A. Wax, C. Yang, V. Backman, K. Badizadegan, C. W. Boone, R. R. Dasari, and M. S. Feld, Biophys. J. 82, 2256 (2002).
[CrossRef] [PubMed]

Boone, C. W.

A. Wax, C. Yang, V. Backman, K. Badizadegan, C. W. Boone, R. R. Dasari, and M. S. Feld, Biophys. J. 82, 2256 (2002).
[CrossRef] [PubMed]

Brock, R. S.

R. S. Brock, X. H. Hu, D. A. Weidner, J. R. Mourant, and J. Q. Lu, J. Quant. Spectrosc. Radiat. Transf. 102, 25 (2006).
[CrossRef]

Carpenter, S.

J. R. Mourant, T. M. Johnson, S. Carpenter, A. Guerra, T. Aida, and J. P. Freyer, J. Biomed. Opt. 7, 378 (2002).
[CrossRef] [PubMed]

Chen, Z.

Crawford, J. M.

L. T. Perelman, V. Backman, M. Wallace, G. Zonios, R. Manoharan, A. Nusrat, S. Shields, M. Seiler, C. Lima, T. Hamano, I. Itzkan, J. Van Dam, J. M. Crawford, and M. S. Feld, Phys. Rev. Lett. 80, 627 (1998).
[CrossRef]

Dam, J. Van

L. T. Perelman, V. Backman, M. Wallace, G. Zonios, R. Manoharan, A. Nusrat, S. Shields, M. Seiler, C. Lima, T. Hamano, I. Itzkan, J. Van Dam, J. M. Crawford, and M. S. Feld, Phys. Rev. Lett. 80, 627 (1998).
[CrossRef]

Dasari, R. R.

A. Wax, C. Yang, V. Backman, K. Badizadegan, C. W. Boone, R. R. Dasari, and M. S. Feld, Biophys. J. 82, 2256 (2002).
[CrossRef] [PubMed]

Drezek, R.

Dunn, A.

A. Dunn, http://www.nmr.mgh.harvard.edu/~adunn/papers/dissertation/node73.html.

Feld, M. S.

A. Wax, C. Yang, V. Backman, K. Badizadegan, C. W. Boone, R. R. Dasari, and M. S. Feld, Biophys. J. 82, 2256 (2002).
[CrossRef] [PubMed]

L. T. Perelman, V. Backman, M. Wallace, G. Zonios, R. Manoharan, A. Nusrat, S. Shields, M. Seiler, C. Lima, T. Hamano, I. Itzkan, J. Van Dam, J. M. Crawford, and M. S. Feld, Phys. Rev. Lett. 80, 627 (1998).
[CrossRef]

Freyer, J. P.

J. R. Mourant, T. M. Johnson, S. Carpenter, A. Guerra, T. Aida, and J. P. Freyer, J. Biomed. Opt. 7, 378 (2002).
[CrossRef] [PubMed]

Gossage, K.

Guerra, A.

J. R. Mourant, T. M. Johnson, S. Carpenter, A. Guerra, T. Aida, and J. P. Freyer, J. Biomed. Opt. 7, 378 (2002).
[CrossRef] [PubMed]

Hamano, T.

L. T. Perelman, V. Backman, M. Wallace, G. Zonios, R. Manoharan, A. Nusrat, S. Shields, M. Seiler, C. Lima, T. Hamano, I. Itzkan, J. Van Dam, J. M. Crawford, and M. S. Feld, Phys. Rev. Lett. 80, 627 (1998).
[CrossRef]

Hu, X. H.

R. S. Brock, X. H. Hu, D. A. Weidner, J. R. Mourant, and J. Q. Lu, J. Quant. Spectrosc. Radiat. Transf. 102, 25 (2006).
[CrossRef]

Itzkan, I.

L. T. Perelman, V. Backman, M. Wallace, G. Zonios, R. Manoharan, A. Nusrat, S. Shields, M. Seiler, C. Lima, T. Hamano, I. Itzkan, J. Van Dam, J. M. Crawford, and M. S. Feld, Phys. Rev. Lett. 80, 627 (1998).
[CrossRef]

Johnson, T. M.

J. R. Mourant, T. M. Johnson, S. Carpenter, A. Guerra, T. Aida, and J. P. Freyer, J. Biomed. Opt. 7, 378 (2002).
[CrossRef] [PubMed]

Jordan, J. A.

M. C. Anderson, J. A. Jordan, A. R. Morse, and F. Sharp, A Text and Atlas of Integrated Colposcopy: for Colposcopists, Histopathologists and Cytologists (Chapman & Hall Medical, 1992).

Keller, J. B.

Lambert, R.

R. Lambert, J. F. Rey, and R. Sankaranarayanan, Endoscopy 35, 437 (2003).
[CrossRef] [PubMed]

Li, X.

X. Li, A. Taflove, and V. Backman, IEEE J. Sel. Top. Quantum Electron. 11, 759 (2005).
[CrossRef]

Lima, C.

L. T. Perelman, V. Backman, M. Wallace, G. Zonios, R. Manoharan, A. Nusrat, S. Shields, M. Seiler, C. Lima, T. Hamano, I. Itzkan, J. Van Dam, J. M. Crawford, and M. S. Feld, Phys. Rev. Lett. 80, 627 (1998).
[CrossRef]

Lu, J. Q.

R. S. Brock, X. H. Hu, D. A. Weidner, J. R. Mourant, and J. Q. Lu, J. Quant. Spectrosc. Radiat. Transf. 102, 25 (2006).
[CrossRef]

Manoharan, R.

L. T. Perelman, V. Backman, M. Wallace, G. Zonios, R. Manoharan, A. Nusrat, S. Shields, M. Seiler, C. Lima, T. Hamano, I. Itzkan, J. Van Dam, J. M. Crawford, and M. S. Feld, Phys. Rev. Lett. 80, 627 (1998).
[CrossRef]

Morse, A. R.

M. C. Anderson, J. A. Jordan, A. R. Morse, and F. Sharp, A Text and Atlas of Integrated Colposcopy: for Colposcopists, Histopathologists and Cytologists (Chapman & Hall Medical, 1992).

Moscoso, M.

Mourant, J. R.

R. S. Brock, X. H. Hu, D. A. Weidner, J. R. Mourant, and J. Q. Lu, J. Quant. Spectrosc. Radiat. Transf. 102, 25 (2006).
[CrossRef]

J. R. Mourant, T. M. Johnson, S. Carpenter, A. Guerra, T. Aida, and J. P. Freyer, J. Biomed. Opt. 7, 378 (2002).
[CrossRef] [PubMed]

Nusrat, A.

L. T. Perelman, V. Backman, M. Wallace, G. Zonios, R. Manoharan, A. Nusrat, S. Shields, M. Seiler, C. Lima, T. Hamano, I. Itzkan, J. Van Dam, J. M. Crawford, and M. S. Feld, Phys. Rev. Lett. 80, 627 (1998).
[CrossRef]

Papanicolaou, G.

Perelman, L. T.

L. T. Perelman, V. Backman, M. Wallace, G. Zonios, R. Manoharan, A. Nusrat, S. Shields, M. Seiler, C. Lima, T. Hamano, I. Itzkan, J. Van Dam, J. M. Crawford, and M. S. Feld, Phys. Rev. Lett. 80, 627 (1998).
[CrossRef]

Qu, J. Y.

T. T. Wu and J. Y. Qu, Appl. Opt. 46, 4834 (2007).
[CrossRef] [PubMed]

M. Xu, T. T. Wu, and J. Y. Qu, Proc. SPIE 6446, 64460Y (2007).
[CrossRef]

Rey, J. F.

R. Lambert, J. F. Rey, and R. Sankaranarayanan, Endoscopy 35, 437 (2003).
[CrossRef] [PubMed]

Richards-Kortum, R.

Sankaranarayanan, R.

R. Lambert, J. F. Rey, and R. Sankaranarayanan, Endoscopy 35, 437 (2003).
[CrossRef] [PubMed]

Seiler, M.

L. T. Perelman, V. Backman, M. Wallace, G. Zonios, R. Manoharan, A. Nusrat, S. Shields, M. Seiler, C. Lima, T. Hamano, I. Itzkan, J. Van Dam, J. M. Crawford, and M. S. Feld, Phys. Rev. Lett. 80, 627 (1998).
[CrossRef]

Sharp, F.

M. C. Anderson, J. A. Jordan, A. R. Morse, and F. Sharp, A Text and Atlas of Integrated Colposcopy: for Colposcopists, Histopathologists and Cytologists (Chapman & Hall Medical, 1992).

Shields, S.

L. T. Perelman, V. Backman, M. Wallace, G. Zonios, R. Manoharan, A. Nusrat, S. Shields, M. Seiler, C. Lima, T. Hamano, I. Itzkan, J. Van Dam, J. M. Crawford, and M. S. Feld, Phys. Rev. Lett. 80, 627 (1998).
[CrossRef]

Sokolov, K.

Taflove, A.

X. Li, A. Taflove, and V. Backman, IEEE J. Sel. Top. Quantum Electron. 11, 759 (2005).
[CrossRef]

Z. Chen, A. Taflove, and V. Backman, Opt. Lett. 28, 765 (2003).
[CrossRef] [PubMed]

Wallace, M.

L. T. Perelman, V. Backman, M. Wallace, G. Zonios, R. Manoharan, A. Nusrat, S. Shields, M. Seiler, C. Lima, T. Hamano, I. Itzkan, J. Van Dam, J. M. Crawford, and M. S. Feld, Phys. Rev. Lett. 80, 627 (1998).
[CrossRef]

Wax, A.

A. Wax, C. Yang, V. Backman, K. Badizadegan, C. W. Boone, R. R. Dasari, and M. S. Feld, Biophys. J. 82, 2256 (2002).
[CrossRef] [PubMed]

Weidner, D. A.

R. S. Brock, X. H. Hu, D. A. Weidner, J. R. Mourant, and J. Q. Lu, J. Quant. Spectrosc. Radiat. Transf. 102, 25 (2006).
[CrossRef]

Wiscombe, W. J.

Wu, T. T.

M. Xu, T. T. Wu, and J. Y. Qu, Proc. SPIE 6446, 64460Y (2007).
[CrossRef]

T. T. Wu and J. Y. Qu, Appl. Opt. 46, 4834 (2007).
[CrossRef] [PubMed]

Xu, M.

M. Xu, T. T. Wu, and J. Y. Qu, Proc. SPIE 6446, 64460Y (2007).
[CrossRef]

M. Xu and R. R. Alfano, Opt. Lett. 30, 3051 (2005).
[CrossRef] [PubMed]

Yang, C.

A. Wax, C. Yang, V. Backman, K. Badizadegan, C. W. Boone, R. R. Dasari, and M. S. Feld, Biophys. J. 82, 2256 (2002).
[CrossRef] [PubMed]

Zonios, G.

L. T. Perelman, V. Backman, M. Wallace, G. Zonios, R. Manoharan, A. Nusrat, S. Shields, M. Seiler, C. Lima, T. Hamano, I. Itzkan, J. Van Dam, J. M. Crawford, and M. S. Feld, Phys. Rev. Lett. 80, 627 (1998).
[CrossRef]

Appl. Opt.

Biophys. J.

A. Wax, C. Yang, V. Backman, K. Badizadegan, C. W. Boone, R. R. Dasari, and M. S. Feld, Biophys. J. 82, 2256 (2002).
[CrossRef] [PubMed]

Endoscopy

R. Lambert, J. F. Rey, and R. Sankaranarayanan, Endoscopy 35, 437 (2003).
[CrossRef] [PubMed]

IEEE J. Sel. Top. Quantum Electron.

X. Li, A. Taflove, and V. Backman, IEEE J. Sel. Top. Quantum Electron. 11, 759 (2005).
[CrossRef]

J. Biomed. Opt.

J. R. Mourant, T. M. Johnson, S. Carpenter, A. Guerra, T. Aida, and J. P. Freyer, J. Biomed. Opt. 7, 378 (2002).
[CrossRef] [PubMed]

J. Opt. Soc. Am. A

J. Quant. Spectrosc. Radiat. Transf.

R. S. Brock, X. H. Hu, D. A. Weidner, J. R. Mourant, and J. Q. Lu, J. Quant. Spectrosc. Radiat. Transf. 102, 25 (2006).
[CrossRef]

Opt. Express

Opt. Lett.

Phys. Rev. Lett.

L. T. Perelman, V. Backman, M. Wallace, G. Zonios, R. Manoharan, A. Nusrat, S. Shields, M. Seiler, C. Lima, T. Hamano, I. Itzkan, J. Van Dam, J. M. Crawford, and M. S. Feld, Phys. Rev. Lett. 80, 627 (1998).
[CrossRef]

Proc. SPIE

M. Xu, T. T. Wu, and J. Y. Qu, Proc. SPIE 6446, 64460Y (2007).
[CrossRef]

Other

M. C. Anderson, J. A. Jordan, A. R. Morse, and F. Sharp, A Text and Atlas of Integrated Colposcopy: for Colposcopists, Histopathologists and Cytologists (Chapman & Hall Medical, 1992).

A. Dunn, http://www.nmr.mgh.harvard.edu/~adunn/papers/dissertation/node73.html.

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Figures (1)

Fig. 1
Fig. 1

Experimental and fitting results from the measurements before the application of acetic acid: (a) angular-dependent light scattering signals (averaged intensity from 400 to 700 nm ); (b)–(g) light scattering spectra at representative angles. After the application of acetic acid: (h) angular-dependent light scattering signals; (i)–(n) light scattering spectra at representative angles. Solid curves, experimental spectra; curves with circles, fitting spectra.

Tables (1)

Tables Icon

Table 1 Fitting Parameters in the Unified Mie and Fractal Model

Equations (2)

Equations on this page are rendered with MathJax. Learn more.

S Cell ( λ , θ ) 2 ¯ = S 0 ( λ , θ ) 2 + S n ( λ , θ ) 2 + S bg ( λ , θ ) 2
S bg ( λ , θ ) 2 = β 2 0 ( 2 π n 0 λ ) l max ( 2 π n 0 λ ) D f 1 x 6 D f [ 1 + 2 ( 1 cos θ ) x 2 ] 2 d x .

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