Abstract

An optical coherence microscopy system based on line illumination and detection is demonstrated. The system uses a Linnik-type interferometer illuminated by a broadband Ti:sapphire laser and detected by a high-speed, line-scan CCD camera. This approach is less sensitive to incoherent scattering and sample motion than full-field imaging. Spatial resolutions of 2μm×3μm (transverse×axial) are achieved. The sensitivity of the system is 93dB with averaging over 30 line scans. En face real time, cellular-level imaging of biological tissues is demonstrated at 2  framess.

© 2007 Optical Society of America

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    [CrossRef]
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2007 (1)

2006 (1)

2005 (1)

2004 (1)

2003 (1)

2002 (2)

2001 (1)

1996 (1)

J. A. Izatt, M. D. Kulkarni, H.-W. Wang, K. Kobayashi, and M. V. Sivak, IEEE J. Quantum Electron. 2, 1017 (1996).
[CrossRef]

1994 (1)

1991 (1)

D. Huang, E. A. Swanson, C. P. Lin, J. S. Schuman, W. G. Stinson, W. Chang, M. R. Hee, T. Flotte, K. Gregory, C. A. Puliafito, and J. G. Fujimoto, Science 254, 1178 (1991).
[CrossRef] [PubMed]

Aguirre, A. D.

Beaurepaire, E.

Boccara, A.-C

Boccara, A.-C.

Boccara, C.

Bouma, B. E.

Chang, W.

D. Huang, E. A. Swanson, C. P. Lin, J. S. Schuman, W. G. Stinson, W. Chang, M. R. Hee, T. Flotte, K. Gregory, C. A. Puliafito, and J. G. Fujimoto, Science 254, 1178 (1991).
[CrossRef] [PubMed]

DiMarzio, C. A.

Dubois, A.

Dwyer, P. J.

Flotte, T.

D. Huang, E. A. Swanson, C. P. Lin, J. S. Schuman, W. G. Stinson, W. Chang, M. R. Hee, T. Flotte, K. Gregory, C. A. Puliafito, and J. G. Fujimoto, Science 254, 1178 (1991).
[CrossRef] [PubMed]

Fujimoto, J. G.

A. D. Aguirre, P. Hsiung, T. H. Ko, I. Hartl, and J. G. Fujimoto, Opt. Lett. 28, 2064 (2003).
[CrossRef] [PubMed]

J. A. Izatt, M. R. Hee, G. M. Owen, E. A. Swanson, and J. G. Fujimoto, Opt. Lett. 19, 590 (1994).
[CrossRef] [PubMed]

D. Huang, E. A. Swanson, C. P. Lin, J. S. Schuman, W. G. Stinson, W. Chang, M. R. Hee, T. Flotte, K. Gregory, C. A. Puliafito, and J. G. Fujimoto, Science 254, 1178 (1991).
[CrossRef] [PubMed]

Gregory, K.

D. Huang, E. A. Swanson, C. P. Lin, J. S. Schuman, W. G. Stinson, W. Chang, M. R. Hee, T. Flotte, K. Gregory, C. A. Puliafito, and J. G. Fujimoto, Science 254, 1178 (1991).
[CrossRef] [PubMed]

Grieve, K.

Hartl, I.

Hee, M. R.

J. A. Izatt, M. R. Hee, G. M. Owen, E. A. Swanson, and J. G. Fujimoto, Opt. Lett. 19, 590 (1994).
[CrossRef] [PubMed]

D. Huang, E. A. Swanson, C. P. Lin, J. S. Schuman, W. G. Stinson, W. Chang, M. R. Hee, T. Flotte, K. Gregory, C. A. Puliafito, and J. G. Fujimoto, Science 254, 1178 (1991).
[CrossRef] [PubMed]

Hsiung, P.

Huang, D.

D. Huang, E. A. Swanson, C. P. Lin, J. S. Schuman, W. G. Stinson, W. Chang, M. R. Hee, T. Flotte, K. Gregory, C. A. Puliafito, and J. G. Fujimoto, Science 254, 1178 (1991).
[CrossRef] [PubMed]

Iftimia, N.

Izatt, J. A.

J. A. Izatt, M. D. Kulkarni, H.-W. Wang, K. Kobayashi, and M. V. Sivak, IEEE J. Quantum Electron. 2, 1017 (1996).
[CrossRef]

J. A. Izatt, M. R. Hee, G. M. Owen, E. A. Swanson, and J. G. Fujimoto, Opt. Lett. 19, 590 (1994).
[CrossRef] [PubMed]

Ko, T. H.

Kobayashi, K.

J. A. Izatt, M. D. Kulkarni, H.-W. Wang, K. Kobayashi, and M. V. Sivak, IEEE J. Quantum Electron. 2, 1017 (1996).
[CrossRef]

Kulkarni, M. D.

J. A. Izatt, M. D. Kulkarni, H.-W. Wang, K. Kobayashi, and M. V. Sivak, IEEE J. Quantum Electron. 2, 1017 (1996).
[CrossRef]

Le Gargasson, J. F.

Lecaque, R.

Lin, C. P.

D. Huang, E. A. Swanson, C. P. Lin, J. S. Schuman, W. G. Stinson, W. Chang, M. R. Hee, T. Flotte, K. Gregory, C. A. Puliafito, and J. G. Fujimoto, Science 254, 1178 (1991).
[CrossRef] [PubMed]

Moneron, G.

Oh, W. Y.

Owen, G. M.

Paques, M.

Puliafito, C. A.

D. Huang, E. A. Swanson, C. P. Lin, J. S. Schuman, W. G. Stinson, W. Chang, M. R. Hee, T. Flotte, K. Gregory, C. A. Puliafito, and J. G. Fujimoto, Science 254, 1178 (1991).
[CrossRef] [PubMed]

Rajadhyaksha, M.

Sahel, J.

Schuman, J. S.

D. Huang, E. A. Swanson, C. P. Lin, J. S. Schuman, W. G. Stinson, W. Chang, M. R. Hee, T. Flotte, K. Gregory, C. A. Puliafito, and J. G. Fujimoto, Science 254, 1178 (1991).
[CrossRef] [PubMed]

Simonutti, M.

Sivak, M. V.

J. A. Izatt, M. D. Kulkarni, H.-W. Wang, K. Kobayashi, and M. V. Sivak, IEEE J. Quantum Electron. 2, 1017 (1996).
[CrossRef]

Stinson, W. G.

D. Huang, E. A. Swanson, C. P. Lin, J. S. Schuman, W. G. Stinson, W. Chang, M. R. Hee, T. Flotte, K. Gregory, C. A. Puliafito, and J. G. Fujimoto, Science 254, 1178 (1991).
[CrossRef] [PubMed]

Swanson, E. A.

J. A. Izatt, M. R. Hee, G. M. Owen, E. A. Swanson, and J. G. Fujimoto, Opt. Lett. 19, 590 (1994).
[CrossRef] [PubMed]

D. Huang, E. A. Swanson, C. P. Lin, J. S. Schuman, W. G. Stinson, W. Chang, M. R. Hee, T. Flotte, K. Gregory, C. A. Puliafito, and J. G. Fujimoto, Science 254, 1178 (1991).
[CrossRef] [PubMed]

Tearney, G. J.

Vabre, L.

Wang, H.-W.

J. A. Izatt, M. D. Kulkarni, H.-W. Wang, K. Kobayashi, and M. V. Sivak, IEEE J. Quantum Electron. 2, 1017 (1996).
[CrossRef]

Yelin, R.

Yun, S. H.

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Figures (3)

Fig. 1
Fig. 1

(a) Schematic of the line-scanning OCM system. SMF, single-mode fiber; CL, cylindrical lens; BS, beam splitter; MO, microscope objectives; ND, neutral density filter; GP, glass plate for dispersion compensation; PZT, piezoelectric transducer; LSCCD, line-scan CCD camera. (b) Measured axial point spread function. (c) Coherence gated image of a U.S. Air Force resolution target.

Fig. 2
Fig. 2

Comparison of system sensitivity versus number of averages ( N ) using line illumination ( R ref = 0.1 % ) and full-field illumination ( R ref = 1 % ) . Dotted lines indicate theoretical values.

Fig. 3
Fig. 3

Line-scanning OCM en face images of a human colon specimen ex vivo at depths of (a) 100 μ m and (b) 150 μ m . Colonic crypt structures can be visualized with individual goblet cells (red arrows). (c) Representaitve en face histology. (d) Three-dimensional segmented rendering of colon specimen with low-scattering crypt lumen (CL) and goblet cells (red arrows). (e) Corresponding cross-sectional histology showing crypt lumen (CL) and goblet cells (red arrows). Bars = 50 μ m .

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