Abstract

We have developed and tested a wide-field coherent anti-Stokes Raman scattering (CARS) microscopy technique, which provides the simultaneous imaging of an extended illuminated area without scanning. This method is based on the non-phase-matching illumination of a sample and imaging of a CARS signal with a CCD camera using conventional microscope optics. We have identified a set of conditions on the illumination and imaging optics, as well as on sample preparation. Imaging of test objects proved high spatial resolution and chemical selectivity of this technique.

© 2007 Optical Society of America

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References

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  1. A. Volkmer, J. Phys. D 38, R59 (2005).
    [CrossRef]
  2. B. S. Hudson, New Laser Techniques for Biophysical Studies, L. J. Mullins, ed., Vol. 6 of Annual Review of Biophysics and Bioengineering (Annual Reviews, 1977), 135-150.
  3. M. D. Duncan, J. Reintjes, and T. J. Manuccia, Opt. Lett. 7, 350 (1982).
    [CrossRef] [PubMed]
  4. C. Ji-Xin, Y. K. Jia, Z. Gengfeng, and X. S. Xie, Biophys. J. 83, 502 (2002).
    [CrossRef]
  5. C. L. Evans, E. O. Potma, M. Puoris'haag, D. Cote, C. P. Lin, and X. S. Xie, Proc. Natl. Acad. Sci. U.S.A. 102, 16807 (2005).
    [CrossRef] [PubMed]
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    [CrossRef]
  7. C. Heinrich, S. Bernet, and M. Ritsch-Marte, New J. Phys. 8, 36 (2006).
    [CrossRef]

2006

C. Heinrich, S. Bernet, and M. Ritsch-Marte, New J. Phys. 8, 36 (2006).
[CrossRef]

2005

A. Volkmer, J. Phys. D 38, R59 (2005).
[CrossRef]

C. L. Evans, E. O. Potma, M. Puoris'haag, D. Cote, C. P. Lin, and X. S. Xie, Proc. Natl. Acad. Sci. U.S.A. 102, 16807 (2005).
[CrossRef] [PubMed]

2004

C. Heinrich, S. Bernet, and M. Ritsch-Marte, Appl. Phys. Lett. 84, 816 (2004).
[CrossRef]

2002

C. Ji-Xin, Y. K. Jia, Z. Gengfeng, and X. S. Xie, Biophys. J. 83, 502 (2002).
[CrossRef]

1982

1977

B. S. Hudson, New Laser Techniques for Biophysical Studies, L. J. Mullins, ed., Vol. 6 of Annual Review of Biophysics and Bioengineering (Annual Reviews, 1977), 135-150.

Bernet, S.

C. Heinrich, S. Bernet, and M. Ritsch-Marte, New J. Phys. 8, 36 (2006).
[CrossRef]

C. Heinrich, S. Bernet, and M. Ritsch-Marte, Appl. Phys. Lett. 84, 816 (2004).
[CrossRef]

Cote, D.

C. L. Evans, E. O. Potma, M. Puoris'haag, D. Cote, C. P. Lin, and X. S. Xie, Proc. Natl. Acad. Sci. U.S.A. 102, 16807 (2005).
[CrossRef] [PubMed]

Duncan, M. D.

Evans, C. L.

C. L. Evans, E. O. Potma, M. Puoris'haag, D. Cote, C. P. Lin, and X. S. Xie, Proc. Natl. Acad. Sci. U.S.A. 102, 16807 (2005).
[CrossRef] [PubMed]

Gengfeng, Z.

C. Ji-Xin, Y. K. Jia, Z. Gengfeng, and X. S. Xie, Biophys. J. 83, 502 (2002).
[CrossRef]

Heinrich, C.

C. Heinrich, S. Bernet, and M. Ritsch-Marte, New J. Phys. 8, 36 (2006).
[CrossRef]

C. Heinrich, S. Bernet, and M. Ritsch-Marte, Appl. Phys. Lett. 84, 816 (2004).
[CrossRef]

Hudson, B. S.

B. S. Hudson, New Laser Techniques for Biophysical Studies, L. J. Mullins, ed., Vol. 6 of Annual Review of Biophysics and Bioengineering (Annual Reviews, 1977), 135-150.

Jia, Y. K.

C. Ji-Xin, Y. K. Jia, Z. Gengfeng, and X. S. Xie, Biophys. J. 83, 502 (2002).
[CrossRef]

Ji-Xin, C.

C. Ji-Xin, Y. K. Jia, Z. Gengfeng, and X. S. Xie, Biophys. J. 83, 502 (2002).
[CrossRef]

Lin, C. P.

C. L. Evans, E. O. Potma, M. Puoris'haag, D. Cote, C. P. Lin, and X. S. Xie, Proc. Natl. Acad. Sci. U.S.A. 102, 16807 (2005).
[CrossRef] [PubMed]

Manuccia, T. J.

Potma, E. O.

C. L. Evans, E. O. Potma, M. Puoris'haag, D. Cote, C. P. Lin, and X. S. Xie, Proc. Natl. Acad. Sci. U.S.A. 102, 16807 (2005).
[CrossRef] [PubMed]

Puoris'haag, M.

C. L. Evans, E. O. Potma, M. Puoris'haag, D. Cote, C. P. Lin, and X. S. Xie, Proc. Natl. Acad. Sci. U.S.A. 102, 16807 (2005).
[CrossRef] [PubMed]

Reintjes, J.

Ritsch-Marte, M.

C. Heinrich, S. Bernet, and M. Ritsch-Marte, New J. Phys. 8, 36 (2006).
[CrossRef]

C. Heinrich, S. Bernet, and M. Ritsch-Marte, Appl. Phys. Lett. 84, 816 (2004).
[CrossRef]

Volkmer, A.

A. Volkmer, J. Phys. D 38, R59 (2005).
[CrossRef]

Xie, X. S.

C. L. Evans, E. O. Potma, M. Puoris'haag, D. Cote, C. P. Lin, and X. S. Xie, Proc. Natl. Acad. Sci. U.S.A. 102, 16807 (2005).
[CrossRef] [PubMed]

C. Ji-Xin, Y. K. Jia, Z. Gengfeng, and X. S. Xie, Biophys. J. 83, 502 (2002).
[CrossRef]

Appl. Phys. Lett.

C. Heinrich, S. Bernet, and M. Ritsch-Marte, Appl. Phys. Lett. 84, 816 (2004).
[CrossRef]

Biophys. J.

C. Ji-Xin, Y. K. Jia, Z. Gengfeng, and X. S. Xie, Biophys. J. 83, 502 (2002).
[CrossRef]

J. Phys. D

A. Volkmer, J. Phys. D 38, R59 (2005).
[CrossRef]

New J. Phys.

C. Heinrich, S. Bernet, and M. Ritsch-Marte, New J. Phys. 8, 36 (2006).
[CrossRef]

Opt. Lett.

Proc. Natl. Acad. Sci. U.S.A.

C. L. Evans, E. O. Potma, M. Puoris'haag, D. Cote, C. P. Lin, and X. S. Xie, Proc. Natl. Acad. Sci. U.S.A. 102, 16807 (2005).
[CrossRef] [PubMed]

Other

B. S. Hudson, New Laser Techniques for Biophysical Studies, L. J. Mullins, ed., Vol. 6 of Annual Review of Biophysics and Bioengineering (Annual Reviews, 1977), 135-150.

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Figures (3)

Fig. 1
Fig. 1

Wide-field illumination geometry. The pump beam goes through the sample positioned on a dichroic mirror. The Stokes is focused by a microscope objective and is reflected from the mirror. The central part of the Stokes beam is blocked by a circular mask. The CARS signal is collected by the same objective.

Fig. 2
Fig. 2

Normalized dependencies of the CARS signals from a polystyrene bead (small scattering object) and a glass coverslip (extended homogeneous material) as a function of the blocked angle of the Stokes beam, α.

Fig. 3
Fig. 3

Images of the glass and polystyrene beads taken at (a) the 3071 cm 1 resonance and (b) out of resonance at 3116 cm 1 (b).

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