Abstract

The use of an optically thin indium–tin–oxide (ITO) electrode is presented for an optoelectric biosensor simultaneously recording optical images and microimpedance to examine time-dependent cellular growth. The transmittance of a 100nm thick ITO electrode layer is approximately the same as the transmittance of a clean glass substrate, whereas the industry-standard Au(47.5nm)Ti(2.5nm) electrode layer drops the transmittance to less than 10% of that of the glass substrate. The simultaneous optoelectric measurements permit determining the correlation of the cell-covered area increase with the microimpedance increase, and the example results obtained for live porcine pulmonary artery endothelial cells delineate the quantitative and comprehensive nature of cellular attachment and spreading to the substrate, which has not been clearly perceived before.

© 2007 Optical Society of America

Full Article  |  PDF Article
OSA Recommended Articles
Integration of Faradaic electrochemical impedance spectroscopy into a scalable surface plasmon biosensor for in tandem detection

Brandon Hong, Alexander Sun, Lin Pang, A. G. Venkatesh, Drew Hall, and Yeshaiahu Fainman
Opt. Express 23(23) 30237-30249 (2015)

Optically trapping tumor cells to assess differentiation and prognosis of cancers

M. Pradhan, S. Pathak, D. Mathur, and U. Ladiwala
Biomed. Opt. Express 7(3) 943-948 (2016)

Time-lapse scanning surface plasmon microscopy of living adherent cells with a radially polarized beam

Lotfi Berguiga, Laura Streppa, Elise Boyer-Provera, Cristina Martinez-Torres, Laurent Schaeffer, Juan Elezgaray, Alain Arneodo, and Françoise Argoul
Appl. Opt. 55(6) 1216-1227 (2016)

References

You do not have subscription access to this journal. Citation lists with outbound citation links are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription

Cited By

You do not have subscription access to this journal. Cited by links are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription

Figures (3)

You do not have subscription access to this journal. Figure files are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription

Equations (4)

You do not have subscription access to this journal. Equations are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription

Metrics

You do not have subscription access to this journal. Article level metrics are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription