Abstract

Combined confocal scanning ophthalmoscopy/en face T-scan-based ultrahigh-resolution optical coherence tomography (OCT) of the human retina in vivo is reported for the first time to our knowledge. The system uses a superluminescent diode-based broadband source, which gives an axial resolution of 3.2μm in the retina. We demonstrate acquisition of T-scan-based OCT B-scan and simultaneous confocal/C-scan images of the human retina of large lateral size (covering a field of up to 20°) at a frame rate of 2Hz.

© 2006 Optical Society of America

Full Article  |  PDF Article

References

  • View by:
  • |
  • |
  • |

  1. D. Huang, E. A. Swanson, C. P. Lin, J. S. Schuman, W. G. Stintson, W. Chang, M. R. Hee, T. Flotte, K. Gregory, C. A. Puliafito, and J. G. Fujimoto, Science 254, 1178 (1991).
    [CrossRef] [PubMed]
  2. A. Gh. Podoleanu, M. Seeger, G. M. Dobre, D. J. Webb, D. A. Jackson, and F. W. Fitzke, J. Biomed. Opt. 3, 12 (1998).
    [CrossRef]
  3. W. Drexler, U. Morgner, R. K. Ghanta, F. X. Kaertner, J. S. Schuman, and J. G. Fujimoto, Nat. Med. 7, 502 (2001).
    [CrossRef] [PubMed]
  4. T. H. Ko, D. C. Adler, J. G. Fujimoto, D. Mamedov, V. Prokhorov, V. Shidlovski, and S. Yakubovich, Opt. Express 12, 2112 (2004).
    [CrossRef] [PubMed]
  5. L. Vabre, A. C. Dubois, and A. C. Boccara, Opt. Lett. 27, 530 (2002).
    [CrossRef]
  6. K. Wiesauer, M. Pircher, E. Götzinger, S. Bauer, R. Engelke, G. Ahrens, G. Grützner, C. K. Hitzenberger, and D. Stifter, Opt. Express 13, 1015 (2005).
    [CrossRef] [PubMed]
  7. A. Gh. Podoleanu and D. A. Jackson, Electron. Lett. 34, 1088 (1998).
    [CrossRef]
  8. M. Mujat, R. C. Chan, B. Cense, B. H. Park, C. Joo, T. Akkin, T. C. Chen, and J. F. de Boer, Opt. Express 13, 9480 (2005).
    [CrossRef] [PubMed]
  9. American National Standards Institute, 'American National Standard for Safe Use of Lasers,' ANSI Z 136-1 (2000).

2005 (2)

2004 (1)

2002 (1)

2001 (1)

W. Drexler, U. Morgner, R. K. Ghanta, F. X. Kaertner, J. S. Schuman, and J. G. Fujimoto, Nat. Med. 7, 502 (2001).
[CrossRef] [PubMed]

1998 (2)

A. Gh. Podoleanu and D. A. Jackson, Electron. Lett. 34, 1088 (1998).
[CrossRef]

A. Gh. Podoleanu, M. Seeger, G. M. Dobre, D. J. Webb, D. A. Jackson, and F. W. Fitzke, J. Biomed. Opt. 3, 12 (1998).
[CrossRef]

1991 (1)

D. Huang, E. A. Swanson, C. P. Lin, J. S. Schuman, W. G. Stintson, W. Chang, M. R. Hee, T. Flotte, K. Gregory, C. A. Puliafito, and J. G. Fujimoto, Science 254, 1178 (1991).
[CrossRef] [PubMed]

Adler, D. C.

Ahrens, G.

Akkin, T.

Bauer, S.

Boccara, A. C.

Cense, B.

Chan, R. C.

Chang, W.

D. Huang, E. A. Swanson, C. P. Lin, J. S. Schuman, W. G. Stintson, W. Chang, M. R. Hee, T. Flotte, K. Gregory, C. A. Puliafito, and J. G. Fujimoto, Science 254, 1178 (1991).
[CrossRef] [PubMed]

Chen, T. C.

de Boer, J. F.

Dobre, G. M.

A. Gh. Podoleanu, M. Seeger, G. M. Dobre, D. J. Webb, D. A. Jackson, and F. W. Fitzke, J. Biomed. Opt. 3, 12 (1998).
[CrossRef]

Drexler, W.

W. Drexler, U. Morgner, R. K. Ghanta, F. X. Kaertner, J. S. Schuman, and J. G. Fujimoto, Nat. Med. 7, 502 (2001).
[CrossRef] [PubMed]

Dubois, A. C.

Engelke, R.

Fitzke, F. W.

A. Gh. Podoleanu, M. Seeger, G. M. Dobre, D. J. Webb, D. A. Jackson, and F. W. Fitzke, J. Biomed. Opt. 3, 12 (1998).
[CrossRef]

Flotte, T.

D. Huang, E. A. Swanson, C. P. Lin, J. S. Schuman, W. G. Stintson, W. Chang, M. R. Hee, T. Flotte, K. Gregory, C. A. Puliafito, and J. G. Fujimoto, Science 254, 1178 (1991).
[CrossRef] [PubMed]

Fujimoto, J. G.

T. H. Ko, D. C. Adler, J. G. Fujimoto, D. Mamedov, V. Prokhorov, V. Shidlovski, and S. Yakubovich, Opt. Express 12, 2112 (2004).
[CrossRef] [PubMed]

W. Drexler, U. Morgner, R. K. Ghanta, F. X. Kaertner, J. S. Schuman, and J. G. Fujimoto, Nat. Med. 7, 502 (2001).
[CrossRef] [PubMed]

D. Huang, E. A. Swanson, C. P. Lin, J. S. Schuman, W. G. Stintson, W. Chang, M. R. Hee, T. Flotte, K. Gregory, C. A. Puliafito, and J. G. Fujimoto, Science 254, 1178 (1991).
[CrossRef] [PubMed]

Ghanta, R. K.

W. Drexler, U. Morgner, R. K. Ghanta, F. X. Kaertner, J. S. Schuman, and J. G. Fujimoto, Nat. Med. 7, 502 (2001).
[CrossRef] [PubMed]

Götzinger, E.

Gregory, K.

D. Huang, E. A. Swanson, C. P. Lin, J. S. Schuman, W. G. Stintson, W. Chang, M. R. Hee, T. Flotte, K. Gregory, C. A. Puliafito, and J. G. Fujimoto, Science 254, 1178 (1991).
[CrossRef] [PubMed]

Grützner, G.

Hee, M. R.

D. Huang, E. A. Swanson, C. P. Lin, J. S. Schuman, W. G. Stintson, W. Chang, M. R. Hee, T. Flotte, K. Gregory, C. A. Puliafito, and J. G. Fujimoto, Science 254, 1178 (1991).
[CrossRef] [PubMed]

Hitzenberger, C. K.

Huang, D.

D. Huang, E. A. Swanson, C. P. Lin, J. S. Schuman, W. G. Stintson, W. Chang, M. R. Hee, T. Flotte, K. Gregory, C. A. Puliafito, and J. G. Fujimoto, Science 254, 1178 (1991).
[CrossRef] [PubMed]

Jackson, D. A.

A. Gh. Podoleanu and D. A. Jackson, Electron. Lett. 34, 1088 (1998).
[CrossRef]

A. Gh. Podoleanu, M. Seeger, G. M. Dobre, D. J. Webb, D. A. Jackson, and F. W. Fitzke, J. Biomed. Opt. 3, 12 (1998).
[CrossRef]

Joo, C.

Kaertner, F. X.

W. Drexler, U. Morgner, R. K. Ghanta, F. X. Kaertner, J. S. Schuman, and J. G. Fujimoto, Nat. Med. 7, 502 (2001).
[CrossRef] [PubMed]

Ko, T. H.

Lin, C. P.

D. Huang, E. A. Swanson, C. P. Lin, J. S. Schuman, W. G. Stintson, W. Chang, M. R. Hee, T. Flotte, K. Gregory, C. A. Puliafito, and J. G. Fujimoto, Science 254, 1178 (1991).
[CrossRef] [PubMed]

Mamedov, D.

Morgner, U.

W. Drexler, U. Morgner, R. K. Ghanta, F. X. Kaertner, J. S. Schuman, and J. G. Fujimoto, Nat. Med. 7, 502 (2001).
[CrossRef] [PubMed]

Mujat, M.

Park, B. H.

Pircher, M.

Podoleanu, A. Gh.

A. Gh. Podoleanu, M. Seeger, G. M. Dobre, D. J. Webb, D. A. Jackson, and F. W. Fitzke, J. Biomed. Opt. 3, 12 (1998).
[CrossRef]

A. Gh. Podoleanu and D. A. Jackson, Electron. Lett. 34, 1088 (1998).
[CrossRef]

Prokhorov, V.

Puliafito, C. A.

D. Huang, E. A. Swanson, C. P. Lin, J. S. Schuman, W. G. Stintson, W. Chang, M. R. Hee, T. Flotte, K. Gregory, C. A. Puliafito, and J. G. Fujimoto, Science 254, 1178 (1991).
[CrossRef] [PubMed]

Schuman, J. S.

W. Drexler, U. Morgner, R. K. Ghanta, F. X. Kaertner, J. S. Schuman, and J. G. Fujimoto, Nat. Med. 7, 502 (2001).
[CrossRef] [PubMed]

D. Huang, E. A. Swanson, C. P. Lin, J. S. Schuman, W. G. Stintson, W. Chang, M. R. Hee, T. Flotte, K. Gregory, C. A. Puliafito, and J. G. Fujimoto, Science 254, 1178 (1991).
[CrossRef] [PubMed]

Seeger, M.

A. Gh. Podoleanu, M. Seeger, G. M. Dobre, D. J. Webb, D. A. Jackson, and F. W. Fitzke, J. Biomed. Opt. 3, 12 (1998).
[CrossRef]

Shidlovski, V.

Stifter, D.

Stintson, W. G.

D. Huang, E. A. Swanson, C. P. Lin, J. S. Schuman, W. G. Stintson, W. Chang, M. R. Hee, T. Flotte, K. Gregory, C. A. Puliafito, and J. G. Fujimoto, Science 254, 1178 (1991).
[CrossRef] [PubMed]

Swanson, E. A.

D. Huang, E. A. Swanson, C. P. Lin, J. S. Schuman, W. G. Stintson, W. Chang, M. R. Hee, T. Flotte, K. Gregory, C. A. Puliafito, and J. G. Fujimoto, Science 254, 1178 (1991).
[CrossRef] [PubMed]

Vabre, L.

Webb, D. J.

A. Gh. Podoleanu, M. Seeger, G. M. Dobre, D. J. Webb, D. A. Jackson, and F. W. Fitzke, J. Biomed. Opt. 3, 12 (1998).
[CrossRef]

Wiesauer, K.

Yakubovich, S.

Electron. Lett. (1)

A. Gh. Podoleanu and D. A. Jackson, Electron. Lett. 34, 1088 (1998).
[CrossRef]

J. Biomed. Opt. (1)

A. Gh. Podoleanu, M. Seeger, G. M. Dobre, D. J. Webb, D. A. Jackson, and F. W. Fitzke, J. Biomed. Opt. 3, 12 (1998).
[CrossRef]

Nat. Med. (1)

W. Drexler, U. Morgner, R. K. Ghanta, F. X. Kaertner, J. S. Schuman, and J. G. Fujimoto, Nat. Med. 7, 502 (2001).
[CrossRef] [PubMed]

Opt. Express (3)

Opt. Lett. (1)

Science (1)

D. Huang, E. A. Swanson, C. P. Lin, J. S. Schuman, W. G. Stintson, W. Chang, M. R. Hee, T. Flotte, K. Gregory, C. A. Puliafito, and J. G. Fujimoto, Science 254, 1178 (1991).
[CrossRef] [PubMed]

Other (1)

American National Standards Institute, 'American National Standard for Safe Use of Lasers,' ANSI Z 136-1 (2000).

Cited By

OSA participates in CrossRef's Cited-By Linking service. Citing articles from OSA journals and other participating publishers are listed here.

Alert me when this article is cited.


Figures (4)

Fig. 1
Fig. 1

Schematic of the instrument.

Fig. 2
Fig. 2

(a) Spectrum of the Broadlighter source. (b) Axial interferometric point-spread function.

Fig. 3
Fig. 3

Pairs of confocal (left) UHR C-scan images (right) of the macular region of the human retina in vivo: (a) the inner limiting membrane (ILM) and the vitreous (V) are visible; (b) the tilt in the C-scan sectioning plane with respect to the macula reveals retinal layers from a B-scan perspective. NFL, nerve fiber layer; GCL, ganglion cell layer; IPL, inner plexiform layer; INL, inner nuclear layer; OPL, outer plexiform layer; ELM, external limiting membrane; IS/OS, junction between inner and outer photoreceptors; RPE, retinal pigment epithelium; CC, choriocapillaris; C, choroid.

Fig. 4
Fig. 4

T-scan-based UHR OCT B-scan of the macular region of the human retina in vivo (for retinal layer labeling, see Fig. 3).

Metrics