Abstract

As a scanning microscope, STimulated Emission Depletion (STED) nanoscopy needs parallelization for fast wide-field imaging. Using well-designed optical lattices for depletion together with wide-field excitation and a fast camera for detection, we achieve large parallelization of STED nanoscopy. Wide field of view super-resolved images are acquired by scanning over a single unit cell of the optical lattice, which can be as small as 290 nm * 290 nm. Optical Lattice STED (OL-STED) imaging is demonstrated with a resolution down to 70 nm at 12.5 frames per second.

© 2014 Optical Society of America

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2013

A. Chmyrov, J. Keller, T. Grotjohann, M. Ratz, E. d’Este, S. Jakobs, C. Eggeling, S. W. Hell, “Nanoscopy with more than 100,000 ‘doughnuts’,” Nat. Methods 10(8), 737–740 (2013).
[CrossRef] [PubMed]

2012

T. Grotjohann, I. Testa, M. Reuss, T. Brakemann, C. Eggeling, S. W. Hell, S. Jakobs, “rsEGFP2 enables fast RESOLFT nanoscopy of living cells,” eLife 1, e00248 (2012).

E. H. Rego, L. Shao, J. J. Macklin, L. Winoto, G. A. Johansson, N. Kamps-Hughes, M. W. Davidson, M. G. L. Gustafsson, “Nonlinear structured-illumination microscopy with a photoswitchable protein reveals cellular structures at 50-nm resolution,” Proc. Natl. Acad. Sci. U. S. A. 109(3), E135–E143 (2012).
[CrossRef] [PubMed]

2011

2010

G. Giannone, E. Hosy, F. Levet, A. Constals, K. Schulze, A. I. Sobolevsky, M. P. Rosconi, E. Gouaux, R. Tampé, D. Choquet, L. Cognet, “Dynamic superresolution imaging of endogenous proteins on living cells at ultra-high density,” Biophys. J. 99(4), 1303–1310 (2010).
[CrossRef] [PubMed]

2009

P. Kner, B. B. Chhun, E. R. Griffis, L. Winoto, M. G. L. Gustafsson, “Super-resolution video microscopy of live cells by structured illumination,” Nat. Methods 6(5), 339–342 (2009).
[CrossRef] [PubMed]

E. Rittweger, K. Y. Han, S. E. Irvine, C. Eggeling, S. W. Hell, “STED microscopy reveals crystal colour centres with nanometric resolution,” Nat. Photonics 3(3), 144–147 (2009).
[CrossRef]

D. Wildanger, J. Bückers, V. Westphal, S. W. Hell, L. Kastrup, “A STED microscope aligned by design,” Opt. Express 17(18), 16100–16110 (2009).
[CrossRef] [PubMed]

S. W. Hell, “Microscopy and its focal switch,” Nat. Methods 6(1), 24–32 (2009).
[CrossRef] [PubMed]

2008

J. Vogelsang, R. Kasper, C. Steinhauer, B. Person, M. Heilemann, M. Sauer, P. Tinnefeld, “A Reducing and oxidizing system minimizes photobleaching and blinking of fluorescent dyes,” Angew. Chem. Int. Ed. Engl. 47(29), 5465–5469 (2008).
[CrossRef] [PubMed]

B. Harke, J. Keller, C. K. Ullal, V. Westphal, A. Schönle, S. W. Hell, “Resolution scaling in STED microscopy,” Opt. Express 16(6), 4154–4162 (2008).
[CrossRef] [PubMed]

J. L. Stay, T. K. Gaylord, “Three-beam-interference lithography: contrast and crystallography,” Appl. Opt. 47(18), 3221–3230 (2008).
[CrossRef] [PubMed]

D. Wildanger, E. Rittweger, L. Kastrup, S. W. Hell, “STED microscopy with a supercontinuum laser source,” Opt. Express 16(13), 9614–9621 (2008).
[CrossRef] [PubMed]

U. V. Nägerl, K. I. Willig, B. Hein, S. W. Hell, T. Bonhoeffer, “Live-cell imaging of dendritic spines by STED microscopy,” Proc. Natl. Acad. Sci. U.S.A. 105(48), 18982–18987 (2008).
[CrossRef] [PubMed]

V. Westphal, S. O. Rizzoli, M. A. Lauterbach, D. Kamin, R. Jahn, S. W. Hell, “Video-rate far-field optical nanoscopy dissects synaptic vesicle movement,” Science 320(5873), 246–249 (2008).
[CrossRef] [PubMed]

J. Fölling, M. Bossi, H. Bock, R. Medda, C. A. Wurm, B. Hein, S. Jakobs, C. Eggeling, S. W. Hell, “Fluorescence nanoscopy by ground-state depletion and single-molecule return,” Nat. Methods 5(11), 943–945 (2008).
[CrossRef] [PubMed]

M. Heilemann, S. van de Linde, M. Schüttpelz, R. Kasper, B. Seefeldt, A. Mukherjee, P. Tinnefeld, M. Sauer, “Subdiffraction-resolution fluorescence imaging with conventional fluorescent probes,” Angew. Chem. Int. Ed. Engl. 47(33), 6172–6176 (2008).
[CrossRef] [PubMed]

2007

G. Donnert, C. Eggeling, S. W. Hell, “Major signal increase in fluorescence microscopy through dark-state relaxation,” Nat. Methods 4(1), 81–86 (2007).
[CrossRef] [PubMed]

2006

A. Sharonov, R. M. Hochstrasser, “Wide-field subdiffraction imaging by accumulated binding of diffusing probes,” Proc. Natl. Acad. Sci. U.S.A. 103(50), 18911–18916 (2006).
[CrossRef] [PubMed]

M. J. Rust, M. Bates, X. Zhuang, “Sub-diffraction-limit imaging by stochastic optical reconstruction microscopy (STORM),” Nat. Methods 3(10), 793–796 (2006).
[CrossRef] [PubMed]

E. Betzig, G. H. Patterson, R. Sougrat, O. W. Lindwasser, S. Olenych, J. S. Bonifacino, M. W. Davidson, J. Lippincott-Schwartz, H. F. Hess, “Imaging intracellular fluorescent proteins at nanometer resolution,” Science 313(5793), 1642–1645 (2006).
[CrossRef] [PubMed]

S. T. Hess, T. P. K. Girirajan, M. D. Mason, “Ultra-High resolution imaging by fluorescence photoactivation localization microscopy,” Biophys. J. 91(11), 4258–4272 (2006).
[CrossRef] [PubMed]

G. Donnert, J. Keller, R. Medda, M. A. Andrei, S. O. Rizzoli, R. Lührmann, R. Jahn, C. Eggeling, S. W. Hell, “Macromolecular-scale resolution in biological fluorescence microscopy,” Proc. Natl. Acad. Sci. U. S. A. 103(31), 11440–11445 (2006).
[CrossRef] [PubMed]

2005

M. Hofmann, C. Eggeling, S. Jakobs, S. W. Hell, “Breaking the diffraction barrier in fluorescence microscopy at low light intensities by using reversibly photoswitchable proteins,” Proc. Natl. Acad. Sci. U. S. A. 102(49), 17565–17569 (2005).
[CrossRef] [PubMed]

M. G. L. Gustafsson, “Nonlinear structured-illumination microscopy: wide-field fluorescence imaging with theoretically unlimited resolution,” Proc. Natl. Acad. Sci. U. S. A. 102(37), 13081–13086 (2005).
[CrossRef] [PubMed]

V. Westphal, S. W. Hell, “Nanoscale resolution in the focal plane of an optical microscope,” Phys. Rev. Lett. 94(14), 143903 (2005).
[CrossRef] [PubMed]

2003

J. I. Cirac, P. Zoller, “Physics. How to manipulate cold atoms,” Science 301(5630), 176–177 (2003).
[CrossRef] [PubMed]

M. P. MacDonald, G. C. Spalding, K. Dholakia, “Microfluidic sorting in an optical lattice,” Nature 426(6965), 421–424 (2003).
[CrossRef] [PubMed]

2002

2000

M. G. L. Gustafsson, “Surpassing the lateral resolution limit by a factor of two using structured illumination microscopy,” J. Microsc. 198(2), 82–87 (2000).
[CrossRef] [PubMed]

1995

S. W. Hell, M. Kroug, “Ground-state-depletion fluorescence microscopy: A concept for breaking the diffraction resolution limit,” Appl. Phys. B 60(5), 495–497 (1995).
[CrossRef]

1994

1993

A. Hemmerich, T. W. Hänsch, “Two-dimesional atomic crystal bound by light,” Phys. Rev. Lett. 70(4), 410–413 (1993).
[CrossRef] [PubMed]

G. Grynberg, B. Lounis, P. Verkerk, J.-Y. Courtois, C. Salomon, “Quantized motion of cold cesium atoms in two- and three-dimensional optical potentials,” Phys. Rev. Lett. 70(15), 2249–2252 (1993).
[CrossRef] [PubMed]

Andrei, M. A.

G. Donnert, J. Keller, R. Medda, M. A. Andrei, S. O. Rizzoli, R. Lührmann, R. Jahn, C. Eggeling, S. W. Hell, “Macromolecular-scale resolution in biological fluorescence microscopy,” Proc. Natl. Acad. Sci. U. S. A. 103(31), 11440–11445 (2006).
[CrossRef] [PubMed]

Bates, M.

M. J. Rust, M. Bates, X. Zhuang, “Sub-diffraction-limit imaging by stochastic optical reconstruction microscopy (STORM),” Nat. Methods 3(10), 793–796 (2006).
[CrossRef] [PubMed]

Betzig, E.

E. Betzig, G. H. Patterson, R. Sougrat, O. W. Lindwasser, S. Olenych, J. S. Bonifacino, M. W. Davidson, J. Lippincott-Schwartz, H. F. Hess, “Imaging intracellular fluorescent proteins at nanometer resolution,” Science 313(5793), 1642–1645 (2006).
[CrossRef] [PubMed]

Bingen, P.

Bock, H.

J. Fölling, M. Bossi, H. Bock, R. Medda, C. A. Wurm, B. Hein, S. Jakobs, C. Eggeling, S. W. Hell, “Fluorescence nanoscopy by ground-state depletion and single-molecule return,” Nat. Methods 5(11), 943–945 (2008).
[CrossRef] [PubMed]

Bonhoeffer, T.

U. V. Nägerl, K. I. Willig, B. Hein, S. W. Hell, T. Bonhoeffer, “Live-cell imaging of dendritic spines by STED microscopy,” Proc. Natl. Acad. Sci. U.S.A. 105(48), 18982–18987 (2008).
[CrossRef] [PubMed]

Bonifacino, J. S.

E. Betzig, G. H. Patterson, R. Sougrat, O. W. Lindwasser, S. Olenych, J. S. Bonifacino, M. W. Davidson, J. Lippincott-Schwartz, H. F. Hess, “Imaging intracellular fluorescent proteins at nanometer resolution,” Science 313(5793), 1642–1645 (2006).
[CrossRef] [PubMed]

Bossi, M.

J. Fölling, M. Bossi, H. Bock, R. Medda, C. A. Wurm, B. Hein, S. Jakobs, C. Eggeling, S. W. Hell, “Fluorescence nanoscopy by ground-state depletion and single-molecule return,” Nat. Methods 5(11), 943–945 (2008).
[CrossRef] [PubMed]

Brakemann, T.

T. Grotjohann, I. Testa, M. Reuss, T. Brakemann, C. Eggeling, S. W. Hell, S. Jakobs, “rsEGFP2 enables fast RESOLFT nanoscopy of living cells,” eLife 1, e00248 (2012).

Bückers, J.

Chhun, B. B.

P. Kner, B. B. Chhun, E. R. Griffis, L. Winoto, M. G. L. Gustafsson, “Super-resolution video microscopy of live cells by structured illumination,” Nat. Methods 6(5), 339–342 (2009).
[CrossRef] [PubMed]

Chmyrov, A.

A. Chmyrov, J. Keller, T. Grotjohann, M. Ratz, E. d’Este, S. Jakobs, C. Eggeling, S. W. Hell, “Nanoscopy with more than 100,000 ‘doughnuts’,” Nat. Methods 10(8), 737–740 (2013).
[CrossRef] [PubMed]

Choquet, D.

G. Giannone, E. Hosy, F. Levet, A. Constals, K. Schulze, A. I. Sobolevsky, M. P. Rosconi, E. Gouaux, R. Tampé, D. Choquet, L. Cognet, “Dynamic superresolution imaging of endogenous proteins on living cells at ultra-high density,” Biophys. J. 99(4), 1303–1310 (2010).
[CrossRef] [PubMed]

Cirac, J. I.

J. I. Cirac, P. Zoller, “Physics. How to manipulate cold atoms,” Science 301(5630), 176–177 (2003).
[CrossRef] [PubMed]

Cognet, L.

G. Giannone, E. Hosy, F. Levet, A. Constals, K. Schulze, A. I. Sobolevsky, M. P. Rosconi, E. Gouaux, R. Tampé, D. Choquet, L. Cognet, “Dynamic superresolution imaging of endogenous proteins on living cells at ultra-high density,” Biophys. J. 99(4), 1303–1310 (2010).
[CrossRef] [PubMed]

Constals, A.

G. Giannone, E. Hosy, F. Levet, A. Constals, K. Schulze, A. I. Sobolevsky, M. P. Rosconi, E. Gouaux, R. Tampé, D. Choquet, L. Cognet, “Dynamic superresolution imaging of endogenous proteins on living cells at ultra-high density,” Biophys. J. 99(4), 1303–1310 (2010).
[CrossRef] [PubMed]

Courtois, J.-Y.

G. Grynberg, B. Lounis, P. Verkerk, J.-Y. Courtois, C. Salomon, “Quantized motion of cold cesium atoms in two- and three-dimensional optical potentials,” Phys. Rev. Lett. 70(15), 2249–2252 (1993).
[CrossRef] [PubMed]

Cremer, C.

d’Este, E.

A. Chmyrov, J. Keller, T. Grotjohann, M. Ratz, E. d’Este, S. Jakobs, C. Eggeling, S. W. Hell, “Nanoscopy with more than 100,000 ‘doughnuts’,” Nat. Methods 10(8), 737–740 (2013).
[CrossRef] [PubMed]

Davidson, M. W.

E. H. Rego, L. Shao, J. J. Macklin, L. Winoto, G. A. Johansson, N. Kamps-Hughes, M. W. Davidson, M. G. L. Gustafsson, “Nonlinear structured-illumination microscopy with a photoswitchable protein reveals cellular structures at 50-nm resolution,” Proc. Natl. Acad. Sci. U. S. A. 109(3), E135–E143 (2012).
[CrossRef] [PubMed]

E. Betzig, G. H. Patterson, R. Sougrat, O. W. Lindwasser, S. Olenych, J. S. Bonifacino, M. W. Davidson, J. Lippincott-Schwartz, H. F. Hess, “Imaging intracellular fluorescent proteins at nanometer resolution,” Science 313(5793), 1642–1645 (2006).
[CrossRef] [PubMed]

Dholakia, K.

M. P. MacDonald, G. C. Spalding, K. Dholakia, “Microfluidic sorting in an optical lattice,” Nature 426(6965), 421–424 (2003).
[CrossRef] [PubMed]

Donnert, G.

G. Donnert, C. Eggeling, S. W. Hell, “Major signal increase in fluorescence microscopy through dark-state relaxation,” Nat. Methods 4(1), 81–86 (2007).
[CrossRef] [PubMed]

G. Donnert, J. Keller, R. Medda, M. A. Andrei, S. O. Rizzoli, R. Lührmann, R. Jahn, C. Eggeling, S. W. Hell, “Macromolecular-scale resolution in biological fluorescence microscopy,” Proc. Natl. Acad. Sci. U. S. A. 103(31), 11440–11445 (2006).
[CrossRef] [PubMed]

Eggeling, C.

A. Chmyrov, J. Keller, T. Grotjohann, M. Ratz, E. d’Este, S. Jakobs, C. Eggeling, S. W. Hell, “Nanoscopy with more than 100,000 ‘doughnuts’,” Nat. Methods 10(8), 737–740 (2013).
[CrossRef] [PubMed]

T. Grotjohann, I. Testa, M. Reuss, T. Brakemann, C. Eggeling, S. W. Hell, S. Jakobs, “rsEGFP2 enables fast RESOLFT nanoscopy of living cells,” eLife 1, e00248 (2012).

E. Rittweger, K. Y. Han, S. E. Irvine, C. Eggeling, S. W. Hell, “STED microscopy reveals crystal colour centres with nanometric resolution,” Nat. Photonics 3(3), 144–147 (2009).
[CrossRef]

J. Fölling, M. Bossi, H. Bock, R. Medda, C. A. Wurm, B. Hein, S. Jakobs, C. Eggeling, S. W. Hell, “Fluorescence nanoscopy by ground-state depletion and single-molecule return,” Nat. Methods 5(11), 943–945 (2008).
[CrossRef] [PubMed]

G. Donnert, C. Eggeling, S. W. Hell, “Major signal increase in fluorescence microscopy through dark-state relaxation,” Nat. Methods 4(1), 81–86 (2007).
[CrossRef] [PubMed]

G. Donnert, J. Keller, R. Medda, M. A. Andrei, S. O. Rizzoli, R. Lührmann, R. Jahn, C. Eggeling, S. W. Hell, “Macromolecular-scale resolution in biological fluorescence microscopy,” Proc. Natl. Acad. Sci. U. S. A. 103(31), 11440–11445 (2006).
[CrossRef] [PubMed]

M. Hofmann, C. Eggeling, S. Jakobs, S. W. Hell, “Breaking the diffraction barrier in fluorescence microscopy at low light intensities by using reversibly photoswitchable proteins,” Proc. Natl. Acad. Sci. U. S. A. 102(49), 17565–17569 (2005).
[CrossRef] [PubMed]

Engelhardt, J.

Fölling, J.

J. Fölling, M. Bossi, H. Bock, R. Medda, C. A. Wurm, B. Hein, S. Jakobs, C. Eggeling, S. W. Hell, “Fluorescence nanoscopy by ground-state depletion and single-molecule return,” Nat. Methods 5(11), 943–945 (2008).
[CrossRef] [PubMed]

Gaylord, T. K.

Giannone, G.

G. Giannone, E. Hosy, F. Levet, A. Constals, K. Schulze, A. I. Sobolevsky, M. P. Rosconi, E. Gouaux, R. Tampé, D. Choquet, L. Cognet, “Dynamic superresolution imaging of endogenous proteins on living cells at ultra-high density,” Biophys. J. 99(4), 1303–1310 (2010).
[CrossRef] [PubMed]

Girirajan, T. P. K.

S. T. Hess, T. P. K. Girirajan, M. D. Mason, “Ultra-High resolution imaging by fluorescence photoactivation localization microscopy,” Biophys. J. 91(11), 4258–4272 (2006).
[CrossRef] [PubMed]

Gouaux, E.

G. Giannone, E. Hosy, F. Levet, A. Constals, K. Schulze, A. I. Sobolevsky, M. P. Rosconi, E. Gouaux, R. Tampé, D. Choquet, L. Cognet, “Dynamic superresolution imaging of endogenous proteins on living cells at ultra-high density,” Biophys. J. 99(4), 1303–1310 (2010).
[CrossRef] [PubMed]

Griffis, E. R.

P. Kner, B. B. Chhun, E. R. Griffis, L. Winoto, M. G. L. Gustafsson, “Super-resolution video microscopy of live cells by structured illumination,” Nat. Methods 6(5), 339–342 (2009).
[CrossRef] [PubMed]

Grotjohann, T.

A. Chmyrov, J. Keller, T. Grotjohann, M. Ratz, E. d’Este, S. Jakobs, C. Eggeling, S. W. Hell, “Nanoscopy with more than 100,000 ‘doughnuts’,” Nat. Methods 10(8), 737–740 (2013).
[CrossRef] [PubMed]

T. Grotjohann, I. Testa, M. Reuss, T. Brakemann, C. Eggeling, S. W. Hell, S. Jakobs, “rsEGFP2 enables fast RESOLFT nanoscopy of living cells,” eLife 1, e00248 (2012).

Grynberg, G.

G. Grynberg, B. Lounis, P. Verkerk, J.-Y. Courtois, C. Salomon, “Quantized motion of cold cesium atoms in two- and three-dimensional optical potentials,” Phys. Rev. Lett. 70(15), 2249–2252 (1993).
[CrossRef] [PubMed]

Gustafsson, M. G. L.

E. H. Rego, L. Shao, J. J. Macklin, L. Winoto, G. A. Johansson, N. Kamps-Hughes, M. W. Davidson, M. G. L. Gustafsson, “Nonlinear structured-illumination microscopy with a photoswitchable protein reveals cellular structures at 50-nm resolution,” Proc. Natl. Acad. Sci. U. S. A. 109(3), E135–E143 (2012).
[CrossRef] [PubMed]

P. Kner, B. B. Chhun, E. R. Griffis, L. Winoto, M. G. L. Gustafsson, “Super-resolution video microscopy of live cells by structured illumination,” Nat. Methods 6(5), 339–342 (2009).
[CrossRef] [PubMed]

M. G. L. Gustafsson, “Nonlinear structured-illumination microscopy: wide-field fluorescence imaging with theoretically unlimited resolution,” Proc. Natl. Acad. Sci. U. S. A. 102(37), 13081–13086 (2005).
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M. G. L. Gustafsson, “Surpassing the lateral resolution limit by a factor of two using structured illumination microscopy,” J. Microsc. 198(2), 82–87 (2000).
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Han, K. Y.

E. Rittweger, K. Y. Han, S. E. Irvine, C. Eggeling, S. W. Hell, “STED microscopy reveals crystal colour centres with nanometric resolution,” Nat. Photonics 3(3), 144–147 (2009).
[CrossRef]

Hänsch, T. W.

A. Hemmerich, T. W. Hänsch, “Two-dimesional atomic crystal bound by light,” Phys. Rev. Lett. 70(4), 410–413 (1993).
[CrossRef] [PubMed]

Harke, B.

Heilemann, M.

M. Heilemann, S. van de Linde, M. Schüttpelz, R. Kasper, B. Seefeldt, A. Mukherjee, P. Tinnefeld, M. Sauer, “Subdiffraction-resolution fluorescence imaging with conventional fluorescent probes,” Angew. Chem. Int. Ed. Engl. 47(33), 6172–6176 (2008).
[CrossRef] [PubMed]

J. Vogelsang, R. Kasper, C. Steinhauer, B. Person, M. Heilemann, M. Sauer, P. Tinnefeld, “A Reducing and oxidizing system minimizes photobleaching and blinking of fluorescent dyes,” Angew. Chem. Int. Ed. Engl. 47(29), 5465–5469 (2008).
[CrossRef] [PubMed]

Hein, B.

J. Fölling, M. Bossi, H. Bock, R. Medda, C. A. Wurm, B. Hein, S. Jakobs, C. Eggeling, S. W. Hell, “Fluorescence nanoscopy by ground-state depletion and single-molecule return,” Nat. Methods 5(11), 943–945 (2008).
[CrossRef] [PubMed]

U. V. Nägerl, K. I. Willig, B. Hein, S. W. Hell, T. Bonhoeffer, “Live-cell imaging of dendritic spines by STED microscopy,” Proc. Natl. Acad. Sci. U.S.A. 105(48), 18982–18987 (2008).
[CrossRef] [PubMed]

Heintzmann, R.

Hell, S. W.

A. Chmyrov, J. Keller, T. Grotjohann, M. Ratz, E. d’Este, S. Jakobs, C. Eggeling, S. W. Hell, “Nanoscopy with more than 100,000 ‘doughnuts’,” Nat. Methods 10(8), 737–740 (2013).
[CrossRef] [PubMed]

T. Grotjohann, I. Testa, M. Reuss, T. Brakemann, C. Eggeling, S. W. Hell, S. Jakobs, “rsEGFP2 enables fast RESOLFT nanoscopy of living cells,” eLife 1, e00248 (2012).

P. Bingen, M. Reuss, J. Engelhardt, S. W. Hell, “Parallelized STED fluorescence nanoscopy,” Opt. Express 19(24), 23716–23726 (2011).
[CrossRef] [PubMed]

E. Rittweger, K. Y. Han, S. E. Irvine, C. Eggeling, S. W. Hell, “STED microscopy reveals crystal colour centres with nanometric resolution,” Nat. Photonics 3(3), 144–147 (2009).
[CrossRef]

S. W. Hell, “Microscopy and its focal switch,” Nat. Methods 6(1), 24–32 (2009).
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D. Wildanger, J. Bückers, V. Westphal, S. W. Hell, L. Kastrup, “A STED microscope aligned by design,” Opt. Express 17(18), 16100–16110 (2009).
[CrossRef] [PubMed]

B. Harke, J. Keller, C. K. Ullal, V. Westphal, A. Schönle, S. W. Hell, “Resolution scaling in STED microscopy,” Opt. Express 16(6), 4154–4162 (2008).
[CrossRef] [PubMed]

J. Fölling, M. Bossi, H. Bock, R. Medda, C. A. Wurm, B. Hein, S. Jakobs, C. Eggeling, S. W. Hell, “Fluorescence nanoscopy by ground-state depletion and single-molecule return,” Nat. Methods 5(11), 943–945 (2008).
[CrossRef] [PubMed]

U. V. Nägerl, K. I. Willig, B. Hein, S. W. Hell, T. Bonhoeffer, “Live-cell imaging of dendritic spines by STED microscopy,” Proc. Natl. Acad. Sci. U.S.A. 105(48), 18982–18987 (2008).
[CrossRef] [PubMed]

V. Westphal, S. O. Rizzoli, M. A. Lauterbach, D. Kamin, R. Jahn, S. W. Hell, “Video-rate far-field optical nanoscopy dissects synaptic vesicle movement,” Science 320(5873), 246–249 (2008).
[CrossRef] [PubMed]

D. Wildanger, E. Rittweger, L. Kastrup, S. W. Hell, “STED microscopy with a supercontinuum laser source,” Opt. Express 16(13), 9614–9621 (2008).
[CrossRef] [PubMed]

G. Donnert, C. Eggeling, S. W. Hell, “Major signal increase in fluorescence microscopy through dark-state relaxation,” Nat. Methods 4(1), 81–86 (2007).
[CrossRef] [PubMed]

G. Donnert, J. Keller, R. Medda, M. A. Andrei, S. O. Rizzoli, R. Lührmann, R. Jahn, C. Eggeling, S. W. Hell, “Macromolecular-scale resolution in biological fluorescence microscopy,” Proc. Natl. Acad. Sci. U. S. A. 103(31), 11440–11445 (2006).
[CrossRef] [PubMed]

V. Westphal, S. W. Hell, “Nanoscale resolution in the focal plane of an optical microscope,” Phys. Rev. Lett. 94(14), 143903 (2005).
[CrossRef] [PubMed]

M. Hofmann, C. Eggeling, S. Jakobs, S. W. Hell, “Breaking the diffraction barrier in fluorescence microscopy at low light intensities by using reversibly photoswitchable proteins,” Proc. Natl. Acad. Sci. U. S. A. 102(49), 17565–17569 (2005).
[CrossRef] [PubMed]

S. W. Hell, M. Kroug, “Ground-state-depletion fluorescence microscopy: A concept for breaking the diffraction resolution limit,” Appl. Phys. B 60(5), 495–497 (1995).
[CrossRef]

S. W. Hell, J. Wichmann, “Breaking the diffraction resolution limit by stimulated emission: stimulated-emission-depletion fluorescence microscopy,” Opt. Lett. 19(11), 780–782 (1994).
[CrossRef] [PubMed]

Hemmerich, A.

A. Hemmerich, T. W. Hänsch, “Two-dimesional atomic crystal bound by light,” Phys. Rev. Lett. 70(4), 410–413 (1993).
[CrossRef] [PubMed]

Hess, H. F.

E. Betzig, G. H. Patterson, R. Sougrat, O. W. Lindwasser, S. Olenych, J. S. Bonifacino, M. W. Davidson, J. Lippincott-Schwartz, H. F. Hess, “Imaging intracellular fluorescent proteins at nanometer resolution,” Science 313(5793), 1642–1645 (2006).
[CrossRef] [PubMed]

Hess, S. T.

S. T. Hess, T. P. K. Girirajan, M. D. Mason, “Ultra-High resolution imaging by fluorescence photoactivation localization microscopy,” Biophys. J. 91(11), 4258–4272 (2006).
[CrossRef] [PubMed]

Hochstrasser, R. M.

A. Sharonov, R. M. Hochstrasser, “Wide-field subdiffraction imaging by accumulated binding of diffusing probes,” Proc. Natl. Acad. Sci. U.S.A. 103(50), 18911–18916 (2006).
[CrossRef] [PubMed]

Hofmann, M.

M. Hofmann, C. Eggeling, S. Jakobs, S. W. Hell, “Breaking the diffraction barrier in fluorescence microscopy at low light intensities by using reversibly photoswitchable proteins,” Proc. Natl. Acad. Sci. U. S. A. 102(49), 17565–17569 (2005).
[CrossRef] [PubMed]

Hosy, E.

G. Giannone, E. Hosy, F. Levet, A. Constals, K. Schulze, A. I. Sobolevsky, M. P. Rosconi, E. Gouaux, R. Tampé, D. Choquet, L. Cognet, “Dynamic superresolution imaging of endogenous proteins on living cells at ultra-high density,” Biophys. J. 99(4), 1303–1310 (2010).
[CrossRef] [PubMed]

Irvine, S. E.

E. Rittweger, K. Y. Han, S. E. Irvine, C. Eggeling, S. W. Hell, “STED microscopy reveals crystal colour centres with nanometric resolution,” Nat. Photonics 3(3), 144–147 (2009).
[CrossRef]

Jahn, R.

V. Westphal, S. O. Rizzoli, M. A. Lauterbach, D. Kamin, R. Jahn, S. W. Hell, “Video-rate far-field optical nanoscopy dissects synaptic vesicle movement,” Science 320(5873), 246–249 (2008).
[CrossRef] [PubMed]

G. Donnert, J. Keller, R. Medda, M. A. Andrei, S. O. Rizzoli, R. Lührmann, R. Jahn, C. Eggeling, S. W. Hell, “Macromolecular-scale resolution in biological fluorescence microscopy,” Proc. Natl. Acad. Sci. U. S. A. 103(31), 11440–11445 (2006).
[CrossRef] [PubMed]

Jakobs, S.

A. Chmyrov, J. Keller, T. Grotjohann, M. Ratz, E. d’Este, S. Jakobs, C. Eggeling, S. W. Hell, “Nanoscopy with more than 100,000 ‘doughnuts’,” Nat. Methods 10(8), 737–740 (2013).
[CrossRef] [PubMed]

T. Grotjohann, I. Testa, M. Reuss, T. Brakemann, C. Eggeling, S. W. Hell, S. Jakobs, “rsEGFP2 enables fast RESOLFT nanoscopy of living cells,” eLife 1, e00248 (2012).

J. Fölling, M. Bossi, H. Bock, R. Medda, C. A. Wurm, B. Hein, S. Jakobs, C. Eggeling, S. W. Hell, “Fluorescence nanoscopy by ground-state depletion and single-molecule return,” Nat. Methods 5(11), 943–945 (2008).
[CrossRef] [PubMed]

M. Hofmann, C. Eggeling, S. Jakobs, S. W. Hell, “Breaking the diffraction barrier in fluorescence microscopy at low light intensities by using reversibly photoswitchable proteins,” Proc. Natl. Acad. Sci. U. S. A. 102(49), 17565–17569 (2005).
[CrossRef] [PubMed]

Johansson, G. A.

E. H. Rego, L. Shao, J. J. Macklin, L. Winoto, G. A. Johansson, N. Kamps-Hughes, M. W. Davidson, M. G. L. Gustafsson, “Nonlinear structured-illumination microscopy with a photoswitchable protein reveals cellular structures at 50-nm resolution,” Proc. Natl. Acad. Sci. U. S. A. 109(3), E135–E143 (2012).
[CrossRef] [PubMed]

Jovin, T. M.

Kamin, D.

V. Westphal, S. O. Rizzoli, M. A. Lauterbach, D. Kamin, R. Jahn, S. W. Hell, “Video-rate far-field optical nanoscopy dissects synaptic vesicle movement,” Science 320(5873), 246–249 (2008).
[CrossRef] [PubMed]

Kamps-Hughes, N.

E. H. Rego, L. Shao, J. J. Macklin, L. Winoto, G. A. Johansson, N. Kamps-Hughes, M. W. Davidson, M. G. L. Gustafsson, “Nonlinear structured-illumination microscopy with a photoswitchable protein reveals cellular structures at 50-nm resolution,” Proc. Natl. Acad. Sci. U. S. A. 109(3), E135–E143 (2012).
[CrossRef] [PubMed]

Kasper, R.

M. Heilemann, S. van de Linde, M. Schüttpelz, R. Kasper, B. Seefeldt, A. Mukherjee, P. Tinnefeld, M. Sauer, “Subdiffraction-resolution fluorescence imaging with conventional fluorescent probes,” Angew. Chem. Int. Ed. Engl. 47(33), 6172–6176 (2008).
[CrossRef] [PubMed]

J. Vogelsang, R. Kasper, C. Steinhauer, B. Person, M. Heilemann, M. Sauer, P. Tinnefeld, “A Reducing and oxidizing system minimizes photobleaching and blinking of fluorescent dyes,” Angew. Chem. Int. Ed. Engl. 47(29), 5465–5469 (2008).
[CrossRef] [PubMed]

Kastrup, L.

Keller, J.

A. Chmyrov, J. Keller, T. Grotjohann, M. Ratz, E. d’Este, S. Jakobs, C. Eggeling, S. W. Hell, “Nanoscopy with more than 100,000 ‘doughnuts’,” Nat. Methods 10(8), 737–740 (2013).
[CrossRef] [PubMed]

B. Harke, J. Keller, C. K. Ullal, V. Westphal, A. Schönle, S. W. Hell, “Resolution scaling in STED microscopy,” Opt. Express 16(6), 4154–4162 (2008).
[CrossRef] [PubMed]

G. Donnert, J. Keller, R. Medda, M. A. Andrei, S. O. Rizzoli, R. Lührmann, R. Jahn, C. Eggeling, S. W. Hell, “Macromolecular-scale resolution in biological fluorescence microscopy,” Proc. Natl. Acad. Sci. U. S. A. 103(31), 11440–11445 (2006).
[CrossRef] [PubMed]

Kner, P.

P. Kner, B. B. Chhun, E. R. Griffis, L. Winoto, M. G. L. Gustafsson, “Super-resolution video microscopy of live cells by structured illumination,” Nat. Methods 6(5), 339–342 (2009).
[CrossRef] [PubMed]

Kroug, M.

S. W. Hell, M. Kroug, “Ground-state-depletion fluorescence microscopy: A concept for breaking the diffraction resolution limit,” Appl. Phys. B 60(5), 495–497 (1995).
[CrossRef]

Lauterbach, M. A.

V. Westphal, S. O. Rizzoli, M. A. Lauterbach, D. Kamin, R. Jahn, S. W. Hell, “Video-rate far-field optical nanoscopy dissects synaptic vesicle movement,” Science 320(5873), 246–249 (2008).
[CrossRef] [PubMed]

Levet, F.

G. Giannone, E. Hosy, F. Levet, A. Constals, K. Schulze, A. I. Sobolevsky, M. P. Rosconi, E. Gouaux, R. Tampé, D. Choquet, L. Cognet, “Dynamic superresolution imaging of endogenous proteins on living cells at ultra-high density,” Biophys. J. 99(4), 1303–1310 (2010).
[CrossRef] [PubMed]

Lindwasser, O. W.

E. Betzig, G. H. Patterson, R. Sougrat, O. W. Lindwasser, S. Olenych, J. S. Bonifacino, M. W. Davidson, J. Lippincott-Schwartz, H. F. Hess, “Imaging intracellular fluorescent proteins at nanometer resolution,” Science 313(5793), 1642–1645 (2006).
[CrossRef] [PubMed]

Lippincott-Schwartz, J.

E. Betzig, G. H. Patterson, R. Sougrat, O. W. Lindwasser, S. Olenych, J. S. Bonifacino, M. W. Davidson, J. Lippincott-Schwartz, H. F. Hess, “Imaging intracellular fluorescent proteins at nanometer resolution,” Science 313(5793), 1642–1645 (2006).
[CrossRef] [PubMed]

Lounis, B.

G. Grynberg, B. Lounis, P. Verkerk, J.-Y. Courtois, C. Salomon, “Quantized motion of cold cesium atoms in two- and three-dimensional optical potentials,” Phys. Rev. Lett. 70(15), 2249–2252 (1993).
[CrossRef] [PubMed]

Lührmann, R.

G. Donnert, J. Keller, R. Medda, M. A. Andrei, S. O. Rizzoli, R. Lührmann, R. Jahn, C. Eggeling, S. W. Hell, “Macromolecular-scale resolution in biological fluorescence microscopy,” Proc. Natl. Acad. Sci. U. S. A. 103(31), 11440–11445 (2006).
[CrossRef] [PubMed]

MacDonald, M. P.

M. P. MacDonald, G. C. Spalding, K. Dholakia, “Microfluidic sorting in an optical lattice,” Nature 426(6965), 421–424 (2003).
[CrossRef] [PubMed]

Macklin, J. J.

E. H. Rego, L. Shao, J. J. Macklin, L. Winoto, G. A. Johansson, N. Kamps-Hughes, M. W. Davidson, M. G. L. Gustafsson, “Nonlinear structured-illumination microscopy with a photoswitchable protein reveals cellular structures at 50-nm resolution,” Proc. Natl. Acad. Sci. U. S. A. 109(3), E135–E143 (2012).
[CrossRef] [PubMed]

Mason, M. D.

S. T. Hess, T. P. K. Girirajan, M. D. Mason, “Ultra-High resolution imaging by fluorescence photoactivation localization microscopy,” Biophys. J. 91(11), 4258–4272 (2006).
[CrossRef] [PubMed]

Medda, R.

J. Fölling, M. Bossi, H. Bock, R. Medda, C. A. Wurm, B. Hein, S. Jakobs, C. Eggeling, S. W. Hell, “Fluorescence nanoscopy by ground-state depletion and single-molecule return,” Nat. Methods 5(11), 943–945 (2008).
[CrossRef] [PubMed]

G. Donnert, J. Keller, R. Medda, M. A. Andrei, S. O. Rizzoli, R. Lührmann, R. Jahn, C. Eggeling, S. W. Hell, “Macromolecular-scale resolution in biological fluorescence microscopy,” Proc. Natl. Acad. Sci. U. S. A. 103(31), 11440–11445 (2006).
[CrossRef] [PubMed]

Mukherjee, A.

M. Heilemann, S. van de Linde, M. Schüttpelz, R. Kasper, B. Seefeldt, A. Mukherjee, P. Tinnefeld, M. Sauer, “Subdiffraction-resolution fluorescence imaging with conventional fluorescent probes,” Angew. Chem. Int. Ed. Engl. 47(33), 6172–6176 (2008).
[CrossRef] [PubMed]

Nägerl, U. V.

U. V. Nägerl, K. I. Willig, B. Hein, S. W. Hell, T. Bonhoeffer, “Live-cell imaging of dendritic spines by STED microscopy,” Proc. Natl. Acad. Sci. U.S.A. 105(48), 18982–18987 (2008).
[CrossRef] [PubMed]

Olenych, S.

E. Betzig, G. H. Patterson, R. Sougrat, O. W. Lindwasser, S. Olenych, J. S. Bonifacino, M. W. Davidson, J. Lippincott-Schwartz, H. F. Hess, “Imaging intracellular fluorescent proteins at nanometer resolution,” Science 313(5793), 1642–1645 (2006).
[CrossRef] [PubMed]

Patterson, G. H.

E. Betzig, G. H. Patterson, R. Sougrat, O. W. Lindwasser, S. Olenych, J. S. Bonifacino, M. W. Davidson, J. Lippincott-Schwartz, H. F. Hess, “Imaging intracellular fluorescent proteins at nanometer resolution,” Science 313(5793), 1642–1645 (2006).
[CrossRef] [PubMed]

Person, B.

J. Vogelsang, R. Kasper, C. Steinhauer, B. Person, M. Heilemann, M. Sauer, P. Tinnefeld, “A Reducing and oxidizing system minimizes photobleaching and blinking of fluorescent dyes,” Angew. Chem. Int. Ed. Engl. 47(29), 5465–5469 (2008).
[CrossRef] [PubMed]

Ratz, M.

A. Chmyrov, J. Keller, T. Grotjohann, M. Ratz, E. d’Este, S. Jakobs, C. Eggeling, S. W. Hell, “Nanoscopy with more than 100,000 ‘doughnuts’,” Nat. Methods 10(8), 737–740 (2013).
[CrossRef] [PubMed]

Rego, E. H.

E. H. Rego, L. Shao, J. J. Macklin, L. Winoto, G. A. Johansson, N. Kamps-Hughes, M. W. Davidson, M. G. L. Gustafsson, “Nonlinear structured-illumination microscopy with a photoswitchable protein reveals cellular structures at 50-nm resolution,” Proc. Natl. Acad. Sci. U. S. A. 109(3), E135–E143 (2012).
[CrossRef] [PubMed]

Reuss, M.

T. Grotjohann, I. Testa, M. Reuss, T. Brakemann, C. Eggeling, S. W. Hell, S. Jakobs, “rsEGFP2 enables fast RESOLFT nanoscopy of living cells,” eLife 1, e00248 (2012).

P. Bingen, M. Reuss, J. Engelhardt, S. W. Hell, “Parallelized STED fluorescence nanoscopy,” Opt. Express 19(24), 23716–23726 (2011).
[CrossRef] [PubMed]

Rittweger, E.

E. Rittweger, K. Y. Han, S. E. Irvine, C. Eggeling, S. W. Hell, “STED microscopy reveals crystal colour centres with nanometric resolution,” Nat. Photonics 3(3), 144–147 (2009).
[CrossRef]

D. Wildanger, E. Rittweger, L. Kastrup, S. W. Hell, “STED microscopy with a supercontinuum laser source,” Opt. Express 16(13), 9614–9621 (2008).
[CrossRef] [PubMed]

Rizzoli, S. O.

V. Westphal, S. O. Rizzoli, M. A. Lauterbach, D. Kamin, R. Jahn, S. W. Hell, “Video-rate far-field optical nanoscopy dissects synaptic vesicle movement,” Science 320(5873), 246–249 (2008).
[CrossRef] [PubMed]

G. Donnert, J. Keller, R. Medda, M. A. Andrei, S. O. Rizzoli, R. Lührmann, R. Jahn, C. Eggeling, S. W. Hell, “Macromolecular-scale resolution in biological fluorescence microscopy,” Proc. Natl. Acad. Sci. U. S. A. 103(31), 11440–11445 (2006).
[CrossRef] [PubMed]

Rosconi, M. P.

G. Giannone, E. Hosy, F. Levet, A. Constals, K. Schulze, A. I. Sobolevsky, M. P. Rosconi, E. Gouaux, R. Tampé, D. Choquet, L. Cognet, “Dynamic superresolution imaging of endogenous proteins on living cells at ultra-high density,” Biophys. J. 99(4), 1303–1310 (2010).
[CrossRef] [PubMed]

Rust, M. J.

M. J. Rust, M. Bates, X. Zhuang, “Sub-diffraction-limit imaging by stochastic optical reconstruction microscopy (STORM),” Nat. Methods 3(10), 793–796 (2006).
[CrossRef] [PubMed]

Salomon, C.

G. Grynberg, B. Lounis, P. Verkerk, J.-Y. Courtois, C. Salomon, “Quantized motion of cold cesium atoms in two- and three-dimensional optical potentials,” Phys. Rev. Lett. 70(15), 2249–2252 (1993).
[CrossRef] [PubMed]

Sauer, M.

M. Heilemann, S. van de Linde, M. Schüttpelz, R. Kasper, B. Seefeldt, A. Mukherjee, P. Tinnefeld, M. Sauer, “Subdiffraction-resolution fluorescence imaging with conventional fluorescent probes,” Angew. Chem. Int. Ed. Engl. 47(33), 6172–6176 (2008).
[CrossRef] [PubMed]

J. Vogelsang, R. Kasper, C. Steinhauer, B. Person, M. Heilemann, M. Sauer, P. Tinnefeld, “A Reducing and oxidizing system minimizes photobleaching and blinking of fluorescent dyes,” Angew. Chem. Int. Ed. Engl. 47(29), 5465–5469 (2008).
[CrossRef] [PubMed]

Schönle, A.

Schulze, K.

G. Giannone, E. Hosy, F. Levet, A. Constals, K. Schulze, A. I. Sobolevsky, M. P. Rosconi, E. Gouaux, R. Tampé, D. Choquet, L. Cognet, “Dynamic superresolution imaging of endogenous proteins on living cells at ultra-high density,” Biophys. J. 99(4), 1303–1310 (2010).
[CrossRef] [PubMed]

Schüttpelz, M.

M. Heilemann, S. van de Linde, M. Schüttpelz, R. Kasper, B. Seefeldt, A. Mukherjee, P. Tinnefeld, M. Sauer, “Subdiffraction-resolution fluorescence imaging with conventional fluorescent probes,” Angew. Chem. Int. Ed. Engl. 47(33), 6172–6176 (2008).
[CrossRef] [PubMed]

Seefeldt, B.

M. Heilemann, S. van de Linde, M. Schüttpelz, R. Kasper, B. Seefeldt, A. Mukherjee, P. Tinnefeld, M. Sauer, “Subdiffraction-resolution fluorescence imaging with conventional fluorescent probes,” Angew. Chem. Int. Ed. Engl. 47(33), 6172–6176 (2008).
[CrossRef] [PubMed]

Shao, L.

E. H. Rego, L. Shao, J. J. Macklin, L. Winoto, G. A. Johansson, N. Kamps-Hughes, M. W. Davidson, M. G. L. Gustafsson, “Nonlinear structured-illumination microscopy with a photoswitchable protein reveals cellular structures at 50-nm resolution,” Proc. Natl. Acad. Sci. U. S. A. 109(3), E135–E143 (2012).
[CrossRef] [PubMed]

Sharonov, A.

A. Sharonov, R. M. Hochstrasser, “Wide-field subdiffraction imaging by accumulated binding of diffusing probes,” Proc. Natl. Acad. Sci. U.S.A. 103(50), 18911–18916 (2006).
[CrossRef] [PubMed]

Sobolevsky, A. I.

G. Giannone, E. Hosy, F. Levet, A. Constals, K. Schulze, A. I. Sobolevsky, M. P. Rosconi, E. Gouaux, R. Tampé, D. Choquet, L. Cognet, “Dynamic superresolution imaging of endogenous proteins on living cells at ultra-high density,” Biophys. J. 99(4), 1303–1310 (2010).
[CrossRef] [PubMed]

Sougrat, R.

E. Betzig, G. H. Patterson, R. Sougrat, O. W. Lindwasser, S. Olenych, J. S. Bonifacino, M. W. Davidson, J. Lippincott-Schwartz, H. F. Hess, “Imaging intracellular fluorescent proteins at nanometer resolution,” Science 313(5793), 1642–1645 (2006).
[CrossRef] [PubMed]

Spalding, G. C.

M. P. MacDonald, G. C. Spalding, K. Dholakia, “Microfluidic sorting in an optical lattice,” Nature 426(6965), 421–424 (2003).
[CrossRef] [PubMed]

Stay, J. L.

Steinhauer, C.

J. Vogelsang, R. Kasper, C. Steinhauer, B. Person, M. Heilemann, M. Sauer, P. Tinnefeld, “A Reducing and oxidizing system minimizes photobleaching and blinking of fluorescent dyes,” Angew. Chem. Int. Ed. Engl. 47(29), 5465–5469 (2008).
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Tampé, R.

G. Giannone, E. Hosy, F. Levet, A. Constals, K. Schulze, A. I. Sobolevsky, M. P. Rosconi, E. Gouaux, R. Tampé, D. Choquet, L. Cognet, “Dynamic superresolution imaging of endogenous proteins on living cells at ultra-high density,” Biophys. J. 99(4), 1303–1310 (2010).
[CrossRef] [PubMed]

Testa, I.

T. Grotjohann, I. Testa, M. Reuss, T. Brakemann, C. Eggeling, S. W. Hell, S. Jakobs, “rsEGFP2 enables fast RESOLFT nanoscopy of living cells,” eLife 1, e00248 (2012).

Tinnefeld, P.

M. Heilemann, S. van de Linde, M. Schüttpelz, R. Kasper, B. Seefeldt, A. Mukherjee, P. Tinnefeld, M. Sauer, “Subdiffraction-resolution fluorescence imaging with conventional fluorescent probes,” Angew. Chem. Int. Ed. Engl. 47(33), 6172–6176 (2008).
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J. Vogelsang, R. Kasper, C. Steinhauer, B. Person, M. Heilemann, M. Sauer, P. Tinnefeld, “A Reducing and oxidizing system minimizes photobleaching and blinking of fluorescent dyes,” Angew. Chem. Int. Ed. Engl. 47(29), 5465–5469 (2008).
[CrossRef] [PubMed]

Ullal, C. K.

van de Linde, S.

M. Heilemann, S. van de Linde, M. Schüttpelz, R. Kasper, B. Seefeldt, A. Mukherjee, P. Tinnefeld, M. Sauer, “Subdiffraction-resolution fluorescence imaging with conventional fluorescent probes,” Angew. Chem. Int. Ed. Engl. 47(33), 6172–6176 (2008).
[CrossRef] [PubMed]

Verkerk, P.

G. Grynberg, B. Lounis, P. Verkerk, J.-Y. Courtois, C. Salomon, “Quantized motion of cold cesium atoms in two- and three-dimensional optical potentials,” Phys. Rev. Lett. 70(15), 2249–2252 (1993).
[CrossRef] [PubMed]

Vogelsang, J.

J. Vogelsang, R. Kasper, C. Steinhauer, B. Person, M. Heilemann, M. Sauer, P. Tinnefeld, “A Reducing and oxidizing system minimizes photobleaching and blinking of fluorescent dyes,” Angew. Chem. Int. Ed. Engl. 47(29), 5465–5469 (2008).
[CrossRef] [PubMed]

Westphal, V.

D. Wildanger, J. Bückers, V. Westphal, S. W. Hell, L. Kastrup, “A STED microscope aligned by design,” Opt. Express 17(18), 16100–16110 (2009).
[CrossRef] [PubMed]

B. Harke, J. Keller, C. K. Ullal, V. Westphal, A. Schönle, S. W. Hell, “Resolution scaling in STED microscopy,” Opt. Express 16(6), 4154–4162 (2008).
[CrossRef] [PubMed]

V. Westphal, S. O. Rizzoli, M. A. Lauterbach, D. Kamin, R. Jahn, S. W. Hell, “Video-rate far-field optical nanoscopy dissects synaptic vesicle movement,” Science 320(5873), 246–249 (2008).
[CrossRef] [PubMed]

V. Westphal, S. W. Hell, “Nanoscale resolution in the focal plane of an optical microscope,” Phys. Rev. Lett. 94(14), 143903 (2005).
[CrossRef] [PubMed]

Wichmann, J.

Wildanger, D.

Willig, K. I.

U. V. Nägerl, K. I. Willig, B. Hein, S. W. Hell, T. Bonhoeffer, “Live-cell imaging of dendritic spines by STED microscopy,” Proc. Natl. Acad. Sci. U.S.A. 105(48), 18982–18987 (2008).
[CrossRef] [PubMed]

Winoto, L.

E. H. Rego, L. Shao, J. J. Macklin, L. Winoto, G. A. Johansson, N. Kamps-Hughes, M. W. Davidson, M. G. L. Gustafsson, “Nonlinear structured-illumination microscopy with a photoswitchable protein reveals cellular structures at 50-nm resolution,” Proc. Natl. Acad. Sci. U. S. A. 109(3), E135–E143 (2012).
[CrossRef] [PubMed]

P. Kner, B. B. Chhun, E. R. Griffis, L. Winoto, M. G. L. Gustafsson, “Super-resolution video microscopy of live cells by structured illumination,” Nat. Methods 6(5), 339–342 (2009).
[CrossRef] [PubMed]

Wurm, C. A.

J. Fölling, M. Bossi, H. Bock, R. Medda, C. A. Wurm, B. Hein, S. Jakobs, C. Eggeling, S. W. Hell, “Fluorescence nanoscopy by ground-state depletion and single-molecule return,” Nat. Methods 5(11), 943–945 (2008).
[CrossRef] [PubMed]

Zhuang, X.

M. J. Rust, M. Bates, X. Zhuang, “Sub-diffraction-limit imaging by stochastic optical reconstruction microscopy (STORM),” Nat. Methods 3(10), 793–796 (2006).
[CrossRef] [PubMed]

Zoller, P.

J. I. Cirac, P. Zoller, “Physics. How to manipulate cold atoms,” Science 301(5630), 176–177 (2003).
[CrossRef] [PubMed]

Angew. Chem. Int. Ed. Engl.

M. Heilemann, S. van de Linde, M. Schüttpelz, R. Kasper, B. Seefeldt, A. Mukherjee, P. Tinnefeld, M. Sauer, “Subdiffraction-resolution fluorescence imaging with conventional fluorescent probes,” Angew. Chem. Int. Ed. Engl. 47(33), 6172–6176 (2008).
[CrossRef] [PubMed]

J. Vogelsang, R. Kasper, C. Steinhauer, B. Person, M. Heilemann, M. Sauer, P. Tinnefeld, “A Reducing and oxidizing system minimizes photobleaching and blinking of fluorescent dyes,” Angew. Chem. Int. Ed. Engl. 47(29), 5465–5469 (2008).
[CrossRef] [PubMed]

Appl. Opt.

Appl. Phys. B

S. W. Hell, M. Kroug, “Ground-state-depletion fluorescence microscopy: A concept for breaking the diffraction resolution limit,” Appl. Phys. B 60(5), 495–497 (1995).
[CrossRef]

Biophys. J.

G. Giannone, E. Hosy, F. Levet, A. Constals, K. Schulze, A. I. Sobolevsky, M. P. Rosconi, E. Gouaux, R. Tampé, D. Choquet, L. Cognet, “Dynamic superresolution imaging of endogenous proteins on living cells at ultra-high density,” Biophys. J. 99(4), 1303–1310 (2010).
[CrossRef] [PubMed]

S. T. Hess, T. P. K. Girirajan, M. D. Mason, “Ultra-High resolution imaging by fluorescence photoactivation localization microscopy,” Biophys. J. 91(11), 4258–4272 (2006).
[CrossRef] [PubMed]

eLife

T. Grotjohann, I. Testa, M. Reuss, T. Brakemann, C. Eggeling, S. W. Hell, S. Jakobs, “rsEGFP2 enables fast RESOLFT nanoscopy of living cells,” eLife 1, e00248 (2012).

J. Microsc.

M. G. L. Gustafsson, “Surpassing the lateral resolution limit by a factor of two using structured illumination microscopy,” J. Microsc. 198(2), 82–87 (2000).
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J. Opt. Soc. Am. A

Nat. Methods

S. W. Hell, “Microscopy and its focal switch,” Nat. Methods 6(1), 24–32 (2009).
[CrossRef] [PubMed]

A. Chmyrov, J. Keller, T. Grotjohann, M. Ratz, E. d’Este, S. Jakobs, C. Eggeling, S. W. Hell, “Nanoscopy with more than 100,000 ‘doughnuts’,” Nat. Methods 10(8), 737–740 (2013).
[CrossRef] [PubMed]

G. Donnert, C. Eggeling, S. W. Hell, “Major signal increase in fluorescence microscopy through dark-state relaxation,” Nat. Methods 4(1), 81–86 (2007).
[CrossRef] [PubMed]

M. J. Rust, M. Bates, X. Zhuang, “Sub-diffraction-limit imaging by stochastic optical reconstruction microscopy (STORM),” Nat. Methods 3(10), 793–796 (2006).
[CrossRef] [PubMed]

J. Fölling, M. Bossi, H. Bock, R. Medda, C. A. Wurm, B. Hein, S. Jakobs, C. Eggeling, S. W. Hell, “Fluorescence nanoscopy by ground-state depletion and single-molecule return,” Nat. Methods 5(11), 943–945 (2008).
[CrossRef] [PubMed]

P. Kner, B. B. Chhun, E. R. Griffis, L. Winoto, M. G. L. Gustafsson, “Super-resolution video microscopy of live cells by structured illumination,” Nat. Methods 6(5), 339–342 (2009).
[CrossRef] [PubMed]

Nat. Photonics

E. Rittweger, K. Y. Han, S. E. Irvine, C. Eggeling, S. W. Hell, “STED microscopy reveals crystal colour centres with nanometric resolution,” Nat. Photonics 3(3), 144–147 (2009).
[CrossRef]

Nature

M. P. MacDonald, G. C. Spalding, K. Dholakia, “Microfluidic sorting in an optical lattice,” Nature 426(6965), 421–424 (2003).
[CrossRef] [PubMed]

Opt. Express

Opt. Lett.

Phys. Rev. Lett.

V. Westphal, S. W. Hell, “Nanoscale resolution in the focal plane of an optical microscope,” Phys. Rev. Lett. 94(14), 143903 (2005).
[CrossRef] [PubMed]

A. Hemmerich, T. W. Hänsch, “Two-dimesional atomic crystal bound by light,” Phys. Rev. Lett. 70(4), 410–413 (1993).
[CrossRef] [PubMed]

G. Grynberg, B. Lounis, P. Verkerk, J.-Y. Courtois, C. Salomon, “Quantized motion of cold cesium atoms in two- and three-dimensional optical potentials,” Phys. Rev. Lett. 70(15), 2249–2252 (1993).
[CrossRef] [PubMed]

Proc. Natl. Acad. Sci. U. S. A.

G. Donnert, J. Keller, R. Medda, M. A. Andrei, S. O. Rizzoli, R. Lührmann, R. Jahn, C. Eggeling, S. W. Hell, “Macromolecular-scale resolution in biological fluorescence microscopy,” Proc. Natl. Acad. Sci. U. S. A. 103(31), 11440–11445 (2006).
[CrossRef] [PubMed]

E. H. Rego, L. Shao, J. J. Macklin, L. Winoto, G. A. Johansson, N. Kamps-Hughes, M. W. Davidson, M. G. L. Gustafsson, “Nonlinear structured-illumination microscopy with a photoswitchable protein reveals cellular structures at 50-nm resolution,” Proc. Natl. Acad. Sci. U. S. A. 109(3), E135–E143 (2012).
[CrossRef] [PubMed]

M. Hofmann, C. Eggeling, S. Jakobs, S. W. Hell, “Breaking the diffraction barrier in fluorescence microscopy at low light intensities by using reversibly photoswitchable proteins,” Proc. Natl. Acad. Sci. U. S. A. 102(49), 17565–17569 (2005).
[CrossRef] [PubMed]

M. G. L. Gustafsson, “Nonlinear structured-illumination microscopy: wide-field fluorescence imaging with theoretically unlimited resolution,” Proc. Natl. Acad. Sci. U. S. A. 102(37), 13081–13086 (2005).
[CrossRef] [PubMed]

Proc. Natl. Acad. Sci. U.S.A.

A. Sharonov, R. M. Hochstrasser, “Wide-field subdiffraction imaging by accumulated binding of diffusing probes,” Proc. Natl. Acad. Sci. U.S.A. 103(50), 18911–18916 (2006).
[CrossRef] [PubMed]

U. V. Nägerl, K. I. Willig, B. Hein, S. W. Hell, T. Bonhoeffer, “Live-cell imaging of dendritic spines by STED microscopy,” Proc. Natl. Acad. Sci. U.S.A. 105(48), 18982–18987 (2008).
[CrossRef] [PubMed]

Science

V. Westphal, S. O. Rizzoli, M. A. Lauterbach, D. Kamin, R. Jahn, S. W. Hell, “Video-rate far-field optical nanoscopy dissects synaptic vesicle movement,” Science 320(5873), 246–249 (2008).
[CrossRef] [PubMed]

E. Betzig, G. H. Patterson, R. Sougrat, O. W. Lindwasser, S. Olenych, J. S. Bonifacino, M. W. Davidson, J. Lippincott-Schwartz, H. F. Hess, “Imaging intracellular fluorescent proteins at nanometer resolution,” Science 313(5793), 1642–1645 (2006).
[CrossRef] [PubMed]

J. I. Cirac, P. Zoller, “Physics. How to manipulate cold atoms,” Science 301(5630), 176–177 (2003).
[CrossRef] [PubMed]

Other

B. Yang, F. Przybilla, M. Mestre, J.-B. Trebbia, and B. Lounis, “Massive parallelization of STED nanoscopy using optical lattices,” arXiv:1307.3833 [physics] (2013).

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