Abstract

A system to measure light scattering from individual cells excited by an acoustic wave was designed, and tests were performed on live Jurkat cells. Cells passing in a laminar stream within a water bath were excited by a focused ultrasound pulse, while the scattered light from a laser beam was monitored at various scattering angles. The cells were modeled as viscoelastic liquid drops, which return to equilibrium via shape oscillations after an acoustically-induced deformation. The Fast Fourier Transform of the scattered light signal was used to extract information about the highly-damped resonant frequencies of the cells, and the detected frequencies are consistent with theoretical predictions.

© 2008 Optical Society of America

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References

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    [CrossRef] [PubMed]
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    [CrossRef] [PubMed]
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    [CrossRef]
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2007 (4)

2005 (3)

J. Novy, P. Becvarova, J. Skorpikova, V. Mornstein, and R. Janisch, "Discrete Fourier Transform-based analysis of HeLa cell microtubules after ultrasonic exposure," Microsc. Res. Tech. 68, 1-5 (2005).
[CrossRef] [PubMed]

S. H. Bloch, R. E. Short, K. W. Ferrara, and E. R. Wisner, "The effect of size on the acoustic response of polymer-shelled contrast agents," Ultrasound Med. Biol. 31, 439-444 (2005).
[CrossRef] [PubMed]

D. B. Khismatullin and G. A. Truskey, "Three-dimensional numerical simulation of receptor-mediated leukocyte adhesion to surfaces: Effects of cell deformability and viscoelasticity," Phys. Fluids 17, 031505 (2005).
[CrossRef]

2004 (1)

L. Haider, P. Snabre, and M. Boynard, "Rheology and Ultrasound Scattering from Aggregated Red Cell Suspensions in Shear Flow," Biophysics J. 87, 2322-2334 (2004).
[CrossRef]

2001 (1)

D. B. Khismatullin and A. Nadim, "Shape oscillations of a viscoelastic drop," Phys. Rev. E 63, 061508 (2001).
[CrossRef]

2000 (1)

C. Dong and X. Lei, "Biomechanics of cell rolling: Shear flow, cell-surface adhesion, and cell deformability," J. Biomech. 33, 35-43 (2000).
[CrossRef]

1990 (1)

R. A. Roy and R. E. Apfel, "Mechanical characterization of microparticles by scattered ultrasound," J. Acoust. Soc. Am. 87, 2332-2341 (1990).
[CrossRef] [PubMed]

1988 (1)

M. S. Roos and R. E. Apfel, "Application of 30-MHz acoustic scattering to the study of human red blood cells," J. Acoust. Soc. Am. 83, 1639-1644 (1988).
[CrossRef] [PubMed]

1980 (1)

E. D. Hirleman, "Laser-based single particle counters for in situ particulate diagnostics," Opt. Eng. 19, 854-860 (1980).

1979 (1)

1968 (1)

C. A. Miller and L. E. Scriven, "The oscillations of a fluid droplet immersed in another fluid," J. Fluid Mech. 32, 417-435 (1968).
[CrossRef]

Apfel, R. E.

R. A. Roy and R. E. Apfel, "Mechanical characterization of microparticles by scattered ultrasound," J. Acoust. Soc. Am. 87, 2332-2341 (1990).
[CrossRef] [PubMed]

M. S. Roos and R. E. Apfel, "Application of 30-MHz acoustic scattering to the study of human red blood cells," J. Acoust. Soc. Am. 83, 1639-1644 (1988).
[CrossRef] [PubMed]

Becvarova, P.

J. Novy, P. Becvarova, J. Skorpikova, V. Mornstein, and R. Janisch, "Discrete Fourier Transform-based analysis of HeLa cell microtubules after ultrasonic exposure," Microsc. Res. Tech. 68, 1-5 (2005).
[CrossRef] [PubMed]

Bigio, I. J.

C. S. Mulvey, A. L. Curtis, S. K. Singh, and I.J. Bigio, "Elastic scattering spectroscopy as a diagnostic tool for apoptosis in cell cultures," IEEE J. Sel. Topics in Quantum Electron. 13, 1663-1670 (2007).
[CrossRef]

Bloch, S. H.

S. H. Bloch, R. E. Short, K. W. Ferrara, and E. R. Wisner, "The effect of size on the acoustic response of polymer-shelled contrast agents," Ultrasound Med. Biol. 31, 439-444 (2005).
[CrossRef] [PubMed]

Boynard, M.

L. Haider, P. Snabre, and M. Boynard, "Rheology and Ultrasound Scattering from Aggregated Red Cell Suspensions in Shear Flow," Biophysics J. 87, 2322-2334 (2004).
[CrossRef]

Cai, X.

Chalut, K. J.

Curtis, A. L.

C. S. Mulvey, A. L. Curtis, S. K. Singh, and I.J. Bigio, "Elastic scattering spectroscopy as a diagnostic tool for apoptosis in cell cultures," IEEE J. Sel. Topics in Quantum Electron. 13, 1663-1670 (2007).
[CrossRef]

Dong, C.

C. Dong and X. Lei, "Biomechanics of cell rolling: Shear flow, cell-surface adhesion, and cell deformability," J. Biomech. 33, 35-43 (2000).
[CrossRef]

Ferrara, K. W.

S. H. Bloch, R. E. Short, K. W. Ferrara, and E. R. Wisner, "The effect of size on the acoustic response of polymer-shelled contrast agents," Ultrasound Med. Biol. 31, 439-444 (2005).
[CrossRef] [PubMed]

Gouesbet, G.

Gréhan, G.

Haider, L.

L. Haider, P. Snabre, and M. Boynard, "Rheology and Ultrasound Scattering from Aggregated Red Cell Suspensions in Shear Flow," Biophysics J. 87, 2322-2334 (2004).
[CrossRef]

Hirleman, E. D.

E. D. Hirleman, "Laser-based single particle counters for in situ particulate diagnostics," Opt. Eng. 19, 854-860 (1980).

Holve, D.

Janisch, R.

J. Novy, P. Becvarova, J. Skorpikova, V. Mornstein, and R. Janisch, "Discrete Fourier Transform-based analysis of HeLa cell microtubules after ultrasonic exposure," Microsc. Res. Tech. 68, 1-5 (2005).
[CrossRef] [PubMed]

Kawakami, Y.

Keener, J. D.

Khismatullin, D. B.

D. B. Khismatullin and G. A. Truskey, "Three-dimensional numerical simulation of receptor-mediated leukocyte adhesion to surfaces: Effects of cell deformability and viscoelasticity," Phys. Fluids 17, 031505 (2005).
[CrossRef]

D. B. Khismatullin and A. Nadim, "Shape oscillations of a viscoelastic drop," Phys. Rev. E 63, 061508 (2001).
[CrossRef]

Lei, X.

C. Dong and X. Lei, "Biomechanics of cell rolling: Shear flow, cell-surface adhesion, and cell deformability," J. Biomech. 33, 35-43 (2000).
[CrossRef]

Micheletto, R.

Miller, C. A.

C. A. Miller and L. E. Scriven, "The oscillations of a fluid droplet immersed in another fluid," J. Fluid Mech. 32, 417-435 (1968).
[CrossRef]

Mornstein, V.

J. Novy, P. Becvarova, J. Skorpikova, V. Mornstein, and R. Janisch, "Discrete Fourier Transform-based analysis of HeLa cell microtubules after ultrasonic exposure," Microsc. Res. Tech. 68, 1-5 (2005).
[CrossRef] [PubMed]

Mulvey, C. S.

C. S. Mulvey, A. L. Curtis, S. K. Singh, and I.J. Bigio, "Elastic scattering spectroscopy as a diagnostic tool for apoptosis in cell cultures," IEEE J. Sel. Topics in Quantum Electron. 13, 1663-1670 (2007).
[CrossRef]

Nadim, A.

D. B. Khismatullin and A. Nadim, "Shape oscillations of a viscoelastic drop," Phys. Rev. E 63, 061508 (2001).
[CrossRef]

Novy, J.

J. Novy, P. Becvarova, J. Skorpikova, V. Mornstein, and R. Janisch, "Discrete Fourier Transform-based analysis of HeLa cell microtubules after ultrasonic exposure," Microsc. Res. Tech. 68, 1-5 (2005).
[CrossRef] [PubMed]

Piga, R.

Pyhtila, J. W.

Ren, K.

Roos, M. S.

M. S. Roos and R. E. Apfel, "Application of 30-MHz acoustic scattering to the study of human red blood cells," J. Acoust. Soc. Am. 83, 1639-1644 (1988).
[CrossRef] [PubMed]

Roy, R. A.

R. A. Roy and R. E. Apfel, "Mechanical characterization of microparticles by scattered ultrasound," J. Acoust. Soc. Am. 87, 2332-2341 (1990).
[CrossRef] [PubMed]

Scriven, L. E.

C. A. Miller and L. E. Scriven, "The oscillations of a fluid droplet immersed in another fluid," J. Fluid Mech. 32, 417-435 (1968).
[CrossRef]

Self, S. A.

Short, R. E.

S. H. Bloch, R. E. Short, K. W. Ferrara, and E. R. Wisner, "The effect of size on the acoustic response of polymer-shelled contrast agents," Ultrasound Med. Biol. 31, 439-444 (2005).
[CrossRef] [PubMed]

Singh, S. K.

C. S. Mulvey, A. L. Curtis, S. K. Singh, and I.J. Bigio, "Elastic scattering spectroscopy as a diagnostic tool for apoptosis in cell cultures," IEEE J. Sel. Topics in Quantum Electron. 13, 1663-1670 (2007).
[CrossRef]

Skorpikova, J.

J. Novy, P. Becvarova, J. Skorpikova, V. Mornstein, and R. Janisch, "Discrete Fourier Transform-based analysis of HeLa cell microtubules after ultrasonic exposure," Microsc. Res. Tech. 68, 1-5 (2005).
[CrossRef] [PubMed]

Snabre, P.

L. Haider, P. Snabre, and M. Boynard, "Rheology and Ultrasound Scattering from Aggregated Red Cell Suspensions in Shear Flow," Biophysics J. 87, 2322-2334 (2004).
[CrossRef]

Truskey, G. A.

D. B. Khismatullin and G. A. Truskey, "Three-dimensional numerical simulation of receptor-mediated leukocyte adhesion to surfaces: Effects of cell deformability and viscoelasticity," Phys. Fluids 17, 031505 (2005).
[CrossRef]

Wax, A.

Wisner, E. R.

S. H. Bloch, R. E. Short, K. W. Ferrara, and E. R. Wisner, "The effect of size on the acoustic response of polymer-shelled contrast agents," Ultrasound Med. Biol. 31, 439-444 (2005).
[CrossRef] [PubMed]

Xu, F.

Appl. Opt. (1)

Biophysics J. (1)

L. Haider, P. Snabre, and M. Boynard, "Rheology and Ultrasound Scattering from Aggregated Red Cell Suspensions in Shear Flow," Biophysics J. 87, 2322-2334 (2004).
[CrossRef]

J. Acoust. Soc. Am. (2)

R. A. Roy and R. E. Apfel, "Mechanical characterization of microparticles by scattered ultrasound," J. Acoust. Soc. Am. 87, 2332-2341 (1990).
[CrossRef] [PubMed]

M. S. Roos and R. E. Apfel, "Application of 30-MHz acoustic scattering to the study of human red blood cells," J. Acoust. Soc. Am. 83, 1639-1644 (1988).
[CrossRef] [PubMed]

J. Biomech. (1)

C. Dong and X. Lei, "Biomechanics of cell rolling: Shear flow, cell-surface adhesion, and cell deformability," J. Biomech. 33, 35-43 (2000).
[CrossRef]

J. Fluid Mech. (1)

C. A. Miller and L. E. Scriven, "The oscillations of a fluid droplet immersed in another fluid," J. Fluid Mech. 32, 417-435 (1968).
[CrossRef]

J. Opt. Soc. Am. A (1)

Microsc. Res. Tech. (1)

J. Novy, P. Becvarova, J. Skorpikova, V. Mornstein, and R. Janisch, "Discrete Fourier Transform-based analysis of HeLa cell microtubules after ultrasonic exposure," Microsc. Res. Tech. 68, 1-5 (2005).
[CrossRef] [PubMed]

Opt. Eng. (1)

E. D. Hirleman, "Laser-based single particle counters for in situ particulate diagnostics," Opt. Eng. 19, 854-860 (1980).

Opt. Express (1)

Opt. Lett. (1)

Phys. Fluids (1)

D. B. Khismatullin and G. A. Truskey, "Three-dimensional numerical simulation of receptor-mediated leukocyte adhesion to surfaces: Effects of cell deformability and viscoelasticity," Phys. Fluids 17, 031505 (2005).
[CrossRef]

Phys. Rev. E (1)

D. B. Khismatullin and A. Nadim, "Shape oscillations of a viscoelastic drop," Phys. Rev. E 63, 061508 (2001).
[CrossRef]

Topics in Quantum Electron. (1)

C. S. Mulvey, A. L. Curtis, S. K. Singh, and I.J. Bigio, "Elastic scattering spectroscopy as a diagnostic tool for apoptosis in cell cultures," IEEE J. Sel. Topics in Quantum Electron. 13, 1663-1670 (2007).
[CrossRef]

Ultrasound Med. Biol. (1)

S. H. Bloch, R. E. Short, K. W. Ferrara, and E. R. Wisner, "The effect of size on the acoustic response of polymer-shelled contrast agents," Ultrasound Med. Biol. 31, 439-444 (2005).
[CrossRef] [PubMed]

Other (4)

C. Bohren and D. R. Huffman, Absorption and Scattering of Light by Small Particles, (Wylie, 1998).
[CrossRef]

G. M. Cooper and R. E. Hausman, The Cell: A Molecular Approach, (ASM Press, 2003).

H. C. Van de Hulst, Light Scattering by Small Particles, (Wylie, 1957).

G. Chen, N. Chen, A. L. Garner, J. Kolb, R. J. Swanson, S. Beebe, R. P. Joshi, and K. H. Schoenbach, "Conductivity in Jurkat cell suspensions after ultrashort electric pulsing," Proc. 3rd Int’l. Workshop on Biological Effect of EMFs, 56-65 (2004).

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Figures (5)

Fig. 1.
Fig. 1.

Laminar flow setup.

Fig. 2.
Fig. 2.

Excitation-detection system.

Fig. 3.
Fig. 3.

Individual Peak.

Fig. 4.
Fig. 4.

FFT of one peak obtained with the ultrasound off and one peak obtained with the ultrasound on.

Fig. 5.
Fig. 5.

Averaged FFTs of 75 peaks obtained with the ultrasound off and 75 peaks obtained with the ultrasound on.

Equations (1)

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ω e l = 2 ( n 1 ) ( 2 n 2 + 4 n + 3 ) μ c ( 2 n + 1 ) ( 1 + E n ) ρ c R 2 λ 1 c , E n = 4 n ( n 1 ) ( n + 2 ) ( 2 n + 1 ) ( 2 n + 3 ) ( 2 n + 5 ) ,

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