Abstract

Advances in the life sciences rely on the ability to observe dynamic processes in live systems and in environments that mimic in-vivo situations. Therefore, new methodological developments have to provide environments that resemble physiologically and clinically relevant conditions as closely as possible. In this work, plasma-induced laser nanosurgery for three-dimensional sample manipulation and sample perturbation is combined with optically sectioning light-sheet based fluorescence microscopy (SPIM) and applied to three-dimensional biological model systems. This means: a) working with a biological system that is not confined to essentially two dimensions like cell cultures on cover glasses, b) gaining intrinsic optical sectioning capabilities by an efficient three-dimensional fluorescence imaging system, and c) using arbitrarily-shaped three-dimensional ablation-patterns by a plasma-induced laser ablation system that prevent damage to surrounding tissues. Spatial levels in our biological applications range from sub-microns during delicate ablation of single microtubules over the confined disruption of cell membranes in an MDCK-cyst to the macroscopic cutting of a millimeter-sized Zebrafish caudal fin with arbitrary three-dimensional ablation patterns. Dynamic processes like laser-induced hemocyte migration can be studied with our SPIM-microscalpel in intact, live embryos.

© 2007 Optical Society of America

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    [CrossRef] [PubMed]
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    [CrossRef] [PubMed]
  3. E. L. Botvinick, V. Venugopalan, J. V. Shah, L. H. Liaw, and M. W. Berns, "Controlled ablation of microtubules using a picosecond laser," Biophys. J. 87, 4203-4212 (2004).
    [CrossRef] [PubMed]
  4. J. Colombelli, E. G. Reynaud, J. Rietdorf, R. Pepperkok, and E. H. K. Stelzer, "In vivo selective cytoskeleton dynamics quantification in interphase cells induced by pulsed ultraviolet laser nanosurgery," Traffic 6, 1093-1102 (2005).
    [CrossRef] [PubMed]
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    [CrossRef] [PubMed]
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    [CrossRef] [PubMed]
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    [CrossRef]
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    [CrossRef]
  23. F. Jankovics, and D. Brunner, "Transiently reorganized microtubules are essential for zippering during dorsal closure in Drosophila melanogaster," Dev. Cell 11, 375-385 (2006).
    [CrossRef] [PubMed]

2007 (3)

H. Dodt, U. Leischner, A. Schierloh, N. Jährling, C. P. Mauch, K. Deininger, J. M. Deussing, M. Eder, W. Zieglgänsberger, and K. Becker, "Ultramicroscopy: three-dimensional visualization of neuronal networks in the whole mouse brain," Nat. Methods 4, 331-336 (2007).
[CrossRef] [PubMed]

P. J. Verveer, J. Swoger, F. Pampaloni, K. Greger, M. Marcello, and E.H.K. Stelzer, "High-resolution three-dimensional imaging of large specimens with light sheet-based microscopy," Nat. Methods 4, 311-313 (2007).
[PubMed]

K. Greger, J. Swoger, and E. H. K. Stelzer, "Basic building units and properties of a fluorescence Single Plane Illumination Microscope (SPIM)," Rev. Sci. Instrum. 78, 023705 (2007).
[CrossRef] [PubMed]

2006 (4)

C. J. Engelbrecht, and E. H. K. Stelzer, "Resolution enhancement in a light-sheet-based microscope (SPIM)," Opt. Lett. 31, 1477-1479 (2006).
[CrossRef] [PubMed]

F. Jankovics, and D. Brunner, "Transiently reorganized microtubules are essential for zippering during dorsal closure in Drosophila melanogaster," Dev. Cell 11, 375-385 (2006).
[CrossRef] [PubMed]

L. G. Griffith, and M. A. Swartz, "Capturing complex 3D tissue physiology in vitro," Nat. Rev. Mol. Cell Biol. 7, 211-224 (2006).
[CrossRef] [PubMed]

S. Kumar, I. Z. Maxwell, A. Heisterkamp, T. R. Polte, T. P. Lele, M. Salanga, E. Mazur, and D. E. Ingber, "Viscoelastic retraction of single living stress fibers and its impact on cell shape, cytoskeletal organization, and extracellular matrix mechanics," Biophys. J. 90, 3762-3773 (2006).
[CrossRef] [PubMed]

2005 (6)

W. Supatto, D. Débarre, B. Moulia, E. Brouzés, J. Martin, E. Farge, and E. Beaurepaire, "In vivo modulation of morphogenetic movements in Drosophila embryos with femtosecond laser pulses," Proc. Natl. Acad. Sci. U S A 102, 1047-1052 (2005).
[CrossRef] [PubMed]

D. B. Edelman and E. W. Keefer, "A cultural renaissance: in vitro cell biology embraces three-dimensional context," Exp. Neurol. 192, 1-6 (2005).
[CrossRef] [PubMed]

J. Colombelli, E. G. Reynaud, J. Rietdorf, R. Pepperkok, and E. H. K. Stelzer, "In vivo selective cytoskeleton dynamics quantification in interphase cells induced by pulsed ultraviolet laser nanosurgery," Traffic 6, 1093-1102 (2005).
[CrossRef] [PubMed]

B. Stramer, W. Wood, M. J. Galko, M. J. Redd, A. Jacinto, S. M. Parkhurst, and P. Martin, "Live imaging of wound inflammation in Drosophila embryos reveals key roles for small GTPases during in vivo cell migration," J. Cell Biol. 168, 567-573 (2005).
[CrossRef] [PubMed]

A. Nimmerjahn, F. Kirchhoff, and F. Helmchen, "Resting microglial cells are highly dynamic surveillants of brain parenchyma in vivo," Science 308, 1314-1318 (2005).
[CrossRef] [PubMed]

R. Farooqui, and G. Fenteany, "Multiple rows of cells behind an epithelial wound edge extend cryptic lamellipodia to collectively drive cell-sheet movement," J. Cell Sci. 118, 51-63 (2005).
[CrossRef]

2004 (3)

E. L. Botvinick, V. Venugopalan, J. V. Shah, L. H. Liaw, and M. W. Berns, "Controlled ablation of microtubules using a picosecond laser," Biophys. J. 87, 4203-4212 (2004).
[CrossRef] [PubMed]

J. Huisken, J. Swoger, F. D. Bene, J. Wittbrodt, and E. H. K. Stelzer, "Optical sectioning deep inside live embryos by selective plane illumination microscopy," Science 305, 1007-1009 (2004).
[CrossRef] [PubMed]

J. Colombelli, S. W. Grill, and E. H. K. Stelzer, "UV diffraction limited nanosurgery of live biological tissues," Rev. Sci. Instrum. 75, 472-478 (2004).
[CrossRef]

2003 (2)

A. Vogel, and V. Venugopalan, "Mechanisms of pulsed laser ablation of biological tissues," Chem. Rev. 103, 577-644 (2003).
[CrossRef] [PubMed]

M. S. Hutson, Y. Tokutake, M. Chang, J. W. Bloor, S. Venakides, D. P. Kiehart, and G. S. Edwards, "Forces for morphogenesis investigated with laser microsurgery and quantitative modeling," Science 300, 145-149 (2003).
[CrossRef] [PubMed]

2002 (1)

L. E. O'Brien, M. M. P. Zegers, and K. E. Mostov, "Opinion: Building epithelial architecture: insights from three-dimensional culture models," Nat. Rev. Mol. Cell Biol. 3, 531-537 (2002).
[CrossRef]

2001 (1)

S. W. Grill, P. Gönczy, E. H. K. Stelzer, and A. A. Hyman, "Polarity controls forces governing asymmetric spindle positioning in the Caenorhabditis elegans embryo," Nature 409, 630-633 (2001).
[CrossRef] [PubMed]

2000 (1)

D. P. Kiehart, C. G. Galbraith, K. A. Edwards, W. L. Rickoll, and R. A. Montague, "Multiple forces contribute to cell sheet morphogenesis for dorsal closure in Drosophila," J. Cell Biol. 149, 471-490 (2000).
[CrossRef] [PubMed]

1999 (1)

1981 (1)

M. W. Berns, J. Aist, J. Edwards, K. Strahs, J. Girton, P. McNeill, J. B. Rattner, M. Kitzes, M. Hammer-Wilson, L. H. Liaw, A. Siemens, M. Koonce, S. Peterson, S. Brenner, J. Burt, R. Walter, P. J. Bryant, D. van Dyk, J. Coulombe, T. Cahill, and G. S. Berns, "Laser microsurgery in cell and developmental biology," Science 213, 505-513 (1981).
[CrossRef] [PubMed]

Aist, J.

M. W. Berns, J. Aist, J. Edwards, K. Strahs, J. Girton, P. McNeill, J. B. Rattner, M. Kitzes, M. Hammer-Wilson, L. H. Liaw, A. Siemens, M. Koonce, S. Peterson, S. Brenner, J. Burt, R. Walter, P. J. Bryant, D. van Dyk, J. Coulombe, T. Cahill, and G. S. Berns, "Laser microsurgery in cell and developmental biology," Science 213, 505-513 (1981).
[CrossRef] [PubMed]

Beaurepaire, E.

W. Supatto, D. Débarre, B. Moulia, E. Brouzés, J. Martin, E. Farge, and E. Beaurepaire, "In vivo modulation of morphogenetic movements in Drosophila embryos with femtosecond laser pulses," Proc. Natl. Acad. Sci. U S A 102, 1047-1052 (2005).
[CrossRef] [PubMed]

Becker, K.

H. Dodt, U. Leischner, A. Schierloh, N. Jährling, C. P. Mauch, K. Deininger, J. M. Deussing, M. Eder, W. Zieglgänsberger, and K. Becker, "Ultramicroscopy: three-dimensional visualization of neuronal networks in the whole mouse brain," Nat. Methods 4, 331-336 (2007).
[CrossRef] [PubMed]

Bene, F. D.

J. Huisken, J. Swoger, F. D. Bene, J. Wittbrodt, and E. H. K. Stelzer, "Optical sectioning deep inside live embryos by selective plane illumination microscopy," Science 305, 1007-1009 (2004).
[CrossRef] [PubMed]

Berns, G. S.

M. W. Berns, J. Aist, J. Edwards, K. Strahs, J. Girton, P. McNeill, J. B. Rattner, M. Kitzes, M. Hammer-Wilson, L. H. Liaw, A. Siemens, M. Koonce, S. Peterson, S. Brenner, J. Burt, R. Walter, P. J. Bryant, D. van Dyk, J. Coulombe, T. Cahill, and G. S. Berns, "Laser microsurgery in cell and developmental biology," Science 213, 505-513 (1981).
[CrossRef] [PubMed]

Berns, M. W.

E. L. Botvinick, V. Venugopalan, J. V. Shah, L. H. Liaw, and M. W. Berns, "Controlled ablation of microtubules using a picosecond laser," Biophys. J. 87, 4203-4212 (2004).
[CrossRef] [PubMed]

M. W. Berns, J. Aist, J. Edwards, K. Strahs, J. Girton, P. McNeill, J. B. Rattner, M. Kitzes, M. Hammer-Wilson, L. H. Liaw, A. Siemens, M. Koonce, S. Peterson, S. Brenner, J. Burt, R. Walter, P. J. Bryant, D. van Dyk, J. Coulombe, T. Cahill, and G. S. Berns, "Laser microsurgery in cell and developmental biology," Science 213, 505-513 (1981).
[CrossRef] [PubMed]

Bloor, J. W.

M. S. Hutson, Y. Tokutake, M. Chang, J. W. Bloor, S. Venakides, D. P. Kiehart, and G. S. Edwards, "Forces for morphogenesis investigated with laser microsurgery and quantitative modeling," Science 300, 145-149 (2003).
[CrossRef] [PubMed]

Botvinick, E. L.

E. L. Botvinick, V. Venugopalan, J. V. Shah, L. H. Liaw, and M. W. Berns, "Controlled ablation of microtubules using a picosecond laser," Biophys. J. 87, 4203-4212 (2004).
[CrossRef] [PubMed]

Brenner, S.

M. W. Berns, J. Aist, J. Edwards, K. Strahs, J. Girton, P. McNeill, J. B. Rattner, M. Kitzes, M. Hammer-Wilson, L. H. Liaw, A. Siemens, M. Koonce, S. Peterson, S. Brenner, J. Burt, R. Walter, P. J. Bryant, D. van Dyk, J. Coulombe, T. Cahill, and G. S. Berns, "Laser microsurgery in cell and developmental biology," Science 213, 505-513 (1981).
[CrossRef] [PubMed]

Brouzés, E.

W. Supatto, D. Débarre, B. Moulia, E. Brouzés, J. Martin, E. Farge, and E. Beaurepaire, "In vivo modulation of morphogenetic movements in Drosophila embryos with femtosecond laser pulses," Proc. Natl. Acad. Sci. U S A 102, 1047-1052 (2005).
[CrossRef] [PubMed]

Brunner, D.

F. Jankovics, and D. Brunner, "Transiently reorganized microtubules are essential for zippering during dorsal closure in Drosophila melanogaster," Dev. Cell 11, 375-385 (2006).
[CrossRef] [PubMed]

Bryant, P. J.

M. W. Berns, J. Aist, J. Edwards, K. Strahs, J. Girton, P. McNeill, J. B. Rattner, M. Kitzes, M. Hammer-Wilson, L. H. Liaw, A. Siemens, M. Koonce, S. Peterson, S. Brenner, J. Burt, R. Walter, P. J. Bryant, D. van Dyk, J. Coulombe, T. Cahill, and G. S. Berns, "Laser microsurgery in cell and developmental biology," Science 213, 505-513 (1981).
[CrossRef] [PubMed]

Burt, J.

M. W. Berns, J. Aist, J. Edwards, K. Strahs, J. Girton, P. McNeill, J. B. Rattner, M. Kitzes, M. Hammer-Wilson, L. H. Liaw, A. Siemens, M. Koonce, S. Peterson, S. Brenner, J. Burt, R. Walter, P. J. Bryant, D. van Dyk, J. Coulombe, T. Cahill, and G. S. Berns, "Laser microsurgery in cell and developmental biology," Science 213, 505-513 (1981).
[CrossRef] [PubMed]

Cahill, T.

M. W. Berns, J. Aist, J. Edwards, K. Strahs, J. Girton, P. McNeill, J. B. Rattner, M. Kitzes, M. Hammer-Wilson, L. H. Liaw, A. Siemens, M. Koonce, S. Peterson, S. Brenner, J. Burt, R. Walter, P. J. Bryant, D. van Dyk, J. Coulombe, T. Cahill, and G. S. Berns, "Laser microsurgery in cell and developmental biology," Science 213, 505-513 (1981).
[CrossRef] [PubMed]

Chang, M.

M. S. Hutson, Y. Tokutake, M. Chang, J. W. Bloor, S. Venakides, D. P. Kiehart, and G. S. Edwards, "Forces for morphogenesis investigated with laser microsurgery and quantitative modeling," Science 300, 145-149 (2003).
[CrossRef] [PubMed]

Colombelli, J.

J. Colombelli, E. G. Reynaud, J. Rietdorf, R. Pepperkok, and E. H. K. Stelzer, "In vivo selective cytoskeleton dynamics quantification in interphase cells induced by pulsed ultraviolet laser nanosurgery," Traffic 6, 1093-1102 (2005).
[CrossRef] [PubMed]

J. Colombelli, S. W. Grill, and E. H. K. Stelzer, "UV diffraction limited nanosurgery of live biological tissues," Rev. Sci. Instrum. 75, 472-478 (2004).
[CrossRef]

Coulombe, J.

M. W. Berns, J. Aist, J. Edwards, K. Strahs, J. Girton, P. McNeill, J. B. Rattner, M. Kitzes, M. Hammer-Wilson, L. H. Liaw, A. Siemens, M. Koonce, S. Peterson, S. Brenner, J. Burt, R. Walter, P. J. Bryant, D. van Dyk, J. Coulombe, T. Cahill, and G. S. Berns, "Laser microsurgery in cell and developmental biology," Science 213, 505-513 (1981).
[CrossRef] [PubMed]

Débarre, D.

W. Supatto, D. Débarre, B. Moulia, E. Brouzés, J. Martin, E. Farge, and E. Beaurepaire, "In vivo modulation of morphogenetic movements in Drosophila embryos with femtosecond laser pulses," Proc. Natl. Acad. Sci. U S A 102, 1047-1052 (2005).
[CrossRef] [PubMed]

Deininger, K.

H. Dodt, U. Leischner, A. Schierloh, N. Jährling, C. P. Mauch, K. Deininger, J. M. Deussing, M. Eder, W. Zieglgänsberger, and K. Becker, "Ultramicroscopy: three-dimensional visualization of neuronal networks in the whole mouse brain," Nat. Methods 4, 331-336 (2007).
[CrossRef] [PubMed]

Deussing, J. M.

H. Dodt, U. Leischner, A. Schierloh, N. Jährling, C. P. Mauch, K. Deininger, J. M. Deussing, M. Eder, W. Zieglgänsberger, and K. Becker, "Ultramicroscopy: three-dimensional visualization of neuronal networks in the whole mouse brain," Nat. Methods 4, 331-336 (2007).
[CrossRef] [PubMed]

Dodt, H.

H. Dodt, U. Leischner, A. Schierloh, N. Jährling, C. P. Mauch, K. Deininger, J. M. Deussing, M. Eder, W. Zieglgänsberger, and K. Becker, "Ultramicroscopy: three-dimensional visualization of neuronal networks in the whole mouse brain," Nat. Methods 4, 331-336 (2007).
[CrossRef] [PubMed]

Edelman, D. B.

D. B. Edelman and E. W. Keefer, "A cultural renaissance: in vitro cell biology embraces three-dimensional context," Exp. Neurol. 192, 1-6 (2005).
[CrossRef] [PubMed]

Eder, M.

H. Dodt, U. Leischner, A. Schierloh, N. Jährling, C. P. Mauch, K. Deininger, J. M. Deussing, M. Eder, W. Zieglgänsberger, and K. Becker, "Ultramicroscopy: three-dimensional visualization of neuronal networks in the whole mouse brain," Nat. Methods 4, 331-336 (2007).
[CrossRef] [PubMed]

Edwards, G. S.

M. S. Hutson, Y. Tokutake, M. Chang, J. W. Bloor, S. Venakides, D. P. Kiehart, and G. S. Edwards, "Forces for morphogenesis investigated with laser microsurgery and quantitative modeling," Science 300, 145-149 (2003).
[CrossRef] [PubMed]

Edwards, J.

M. W. Berns, J. Aist, J. Edwards, K. Strahs, J. Girton, P. McNeill, J. B. Rattner, M. Kitzes, M. Hammer-Wilson, L. H. Liaw, A. Siemens, M. Koonce, S. Peterson, S. Brenner, J. Burt, R. Walter, P. J. Bryant, D. van Dyk, J. Coulombe, T. Cahill, and G. S. Berns, "Laser microsurgery in cell and developmental biology," Science 213, 505-513 (1981).
[CrossRef] [PubMed]

Edwards, K. A.

D. P. Kiehart, C. G. Galbraith, K. A. Edwards, W. L. Rickoll, and R. A. Montague, "Multiple forces contribute to cell sheet morphogenesis for dorsal closure in Drosophila," J. Cell Biol. 149, 471-490 (2000).
[CrossRef] [PubMed]

Engelbrecht, C. J.

Farge, E.

W. Supatto, D. Débarre, B. Moulia, E. Brouzés, J. Martin, E. Farge, and E. Beaurepaire, "In vivo modulation of morphogenetic movements in Drosophila embryos with femtosecond laser pulses," Proc. Natl. Acad. Sci. U S A 102, 1047-1052 (2005).
[CrossRef] [PubMed]

Farooqui, R.

R. Farooqui, and G. Fenteany, "Multiple rows of cells behind an epithelial wound edge extend cryptic lamellipodia to collectively drive cell-sheet movement," J. Cell Sci. 118, 51-63 (2005).
[CrossRef]

Fenteany, G.

R. Farooqui, and G. Fenteany, "Multiple rows of cells behind an epithelial wound edge extend cryptic lamellipodia to collectively drive cell-sheet movement," J. Cell Sci. 118, 51-63 (2005).
[CrossRef]

Galbraith, C. G.

D. P. Kiehart, C. G. Galbraith, K. A. Edwards, W. L. Rickoll, and R. A. Montague, "Multiple forces contribute to cell sheet morphogenesis for dorsal closure in Drosophila," J. Cell Biol. 149, 471-490 (2000).
[CrossRef] [PubMed]

Galko, M. J.

B. Stramer, W. Wood, M. J. Galko, M. J. Redd, A. Jacinto, S. M. Parkhurst, and P. Martin, "Live imaging of wound inflammation in Drosophila embryos reveals key roles for small GTPases during in vivo cell migration," J. Cell Biol. 168, 567-573 (2005).
[CrossRef] [PubMed]

Girton, J.

M. W. Berns, J. Aist, J. Edwards, K. Strahs, J. Girton, P. McNeill, J. B. Rattner, M. Kitzes, M. Hammer-Wilson, L. H. Liaw, A. Siemens, M. Koonce, S. Peterson, S. Brenner, J. Burt, R. Walter, P. J. Bryant, D. van Dyk, J. Coulombe, T. Cahill, and G. S. Berns, "Laser microsurgery in cell and developmental biology," Science 213, 505-513 (1981).
[CrossRef] [PubMed]

Gönczy, P.

S. W. Grill, P. Gönczy, E. H. K. Stelzer, and A. A. Hyman, "Polarity controls forces governing asymmetric spindle positioning in the Caenorhabditis elegans embryo," Nature 409, 630-633 (2001).
[CrossRef] [PubMed]

Greger, K.

P. J. Verveer, J. Swoger, F. Pampaloni, K. Greger, M. Marcello, and E.H.K. Stelzer, "High-resolution three-dimensional imaging of large specimens with light sheet-based microscopy," Nat. Methods 4, 311-313 (2007).
[PubMed]

K. Greger, J. Swoger, and E. H. K. Stelzer, "Basic building units and properties of a fluorescence Single Plane Illumination Microscope (SPIM)," Rev. Sci. Instrum. 78, 023705 (2007).
[CrossRef] [PubMed]

Griffith, L. G.

L. G. Griffith, and M. A. Swartz, "Capturing complex 3D tissue physiology in vitro," Nat. Rev. Mol. Cell Biol. 7, 211-224 (2006).
[CrossRef] [PubMed]

Grill, S. W.

J. Colombelli, S. W. Grill, and E. H. K. Stelzer, "UV diffraction limited nanosurgery of live biological tissues," Rev. Sci. Instrum. 75, 472-478 (2004).
[CrossRef]

S. W. Grill, P. Gönczy, E. H. K. Stelzer, and A. A. Hyman, "Polarity controls forces governing asymmetric spindle positioning in the Caenorhabditis elegans embryo," Nature 409, 630-633 (2001).
[CrossRef] [PubMed]

S. W. Grill, and E.H. K. Stelzer, "Method to calculate lateral and axial gain factors of optical setups with a large solid angle," J. Opt. Soc. Am. A 16, 2658-2665 (1999).
[CrossRef]

Hammer-Wilson, M.

M. W. Berns, J. Aist, J. Edwards, K. Strahs, J. Girton, P. McNeill, J. B. Rattner, M. Kitzes, M. Hammer-Wilson, L. H. Liaw, A. Siemens, M. Koonce, S. Peterson, S. Brenner, J. Burt, R. Walter, P. J. Bryant, D. van Dyk, J. Coulombe, T. Cahill, and G. S. Berns, "Laser microsurgery in cell and developmental biology," Science 213, 505-513 (1981).
[CrossRef] [PubMed]

Heisterkamp, A.

S. Kumar, I. Z. Maxwell, A. Heisterkamp, T. R. Polte, T. P. Lele, M. Salanga, E. Mazur, and D. E. Ingber, "Viscoelastic retraction of single living stress fibers and its impact on cell shape, cytoskeletal organization, and extracellular matrix mechanics," Biophys. J. 90, 3762-3773 (2006).
[CrossRef] [PubMed]

Helmchen, F.

A. Nimmerjahn, F. Kirchhoff, and F. Helmchen, "Resting microglial cells are highly dynamic surveillants of brain parenchyma in vivo," Science 308, 1314-1318 (2005).
[CrossRef] [PubMed]

Huisken, J.

J. Huisken, J. Swoger, F. D. Bene, J. Wittbrodt, and E. H. K. Stelzer, "Optical sectioning deep inside live embryos by selective plane illumination microscopy," Science 305, 1007-1009 (2004).
[CrossRef] [PubMed]

Hutson, M. S.

M. S. Hutson, Y. Tokutake, M. Chang, J. W. Bloor, S. Venakides, D. P. Kiehart, and G. S. Edwards, "Forces for morphogenesis investigated with laser microsurgery and quantitative modeling," Science 300, 145-149 (2003).
[CrossRef] [PubMed]

Hyman, A. A.

S. W. Grill, P. Gönczy, E. H. K. Stelzer, and A. A. Hyman, "Polarity controls forces governing asymmetric spindle positioning in the Caenorhabditis elegans embryo," Nature 409, 630-633 (2001).
[CrossRef] [PubMed]

Ingber, D. E.

S. Kumar, I. Z. Maxwell, A. Heisterkamp, T. R. Polte, T. P. Lele, M. Salanga, E. Mazur, and D. E. Ingber, "Viscoelastic retraction of single living stress fibers and its impact on cell shape, cytoskeletal organization, and extracellular matrix mechanics," Biophys. J. 90, 3762-3773 (2006).
[CrossRef] [PubMed]

Jacinto, A.

B. Stramer, W. Wood, M. J. Galko, M. J. Redd, A. Jacinto, S. M. Parkhurst, and P. Martin, "Live imaging of wound inflammation in Drosophila embryos reveals key roles for small GTPases during in vivo cell migration," J. Cell Biol. 168, 567-573 (2005).
[CrossRef] [PubMed]

Jährling, N.

H. Dodt, U. Leischner, A. Schierloh, N. Jährling, C. P. Mauch, K. Deininger, J. M. Deussing, M. Eder, W. Zieglgänsberger, and K. Becker, "Ultramicroscopy: three-dimensional visualization of neuronal networks in the whole mouse brain," Nat. Methods 4, 331-336 (2007).
[CrossRef] [PubMed]

Jankovics, F.

F. Jankovics, and D. Brunner, "Transiently reorganized microtubules are essential for zippering during dorsal closure in Drosophila melanogaster," Dev. Cell 11, 375-385 (2006).
[CrossRef] [PubMed]

Keefer, E. W.

D. B. Edelman and E. W. Keefer, "A cultural renaissance: in vitro cell biology embraces three-dimensional context," Exp. Neurol. 192, 1-6 (2005).
[CrossRef] [PubMed]

Kiehart, D. P.

M. S. Hutson, Y. Tokutake, M. Chang, J. W. Bloor, S. Venakides, D. P. Kiehart, and G. S. Edwards, "Forces for morphogenesis investigated with laser microsurgery and quantitative modeling," Science 300, 145-149 (2003).
[CrossRef] [PubMed]

D. P. Kiehart, C. G. Galbraith, K. A. Edwards, W. L. Rickoll, and R. A. Montague, "Multiple forces contribute to cell sheet morphogenesis for dorsal closure in Drosophila," J. Cell Biol. 149, 471-490 (2000).
[CrossRef] [PubMed]

Kirchhoff, F.

A. Nimmerjahn, F. Kirchhoff, and F. Helmchen, "Resting microglial cells are highly dynamic surveillants of brain parenchyma in vivo," Science 308, 1314-1318 (2005).
[CrossRef] [PubMed]

Kitzes, M.

M. W. Berns, J. Aist, J. Edwards, K. Strahs, J. Girton, P. McNeill, J. B. Rattner, M. Kitzes, M. Hammer-Wilson, L. H. Liaw, A. Siemens, M. Koonce, S. Peterson, S. Brenner, J. Burt, R. Walter, P. J. Bryant, D. van Dyk, J. Coulombe, T. Cahill, and G. S. Berns, "Laser microsurgery in cell and developmental biology," Science 213, 505-513 (1981).
[CrossRef] [PubMed]

Koonce, M.

M. W. Berns, J. Aist, J. Edwards, K. Strahs, J. Girton, P. McNeill, J. B. Rattner, M. Kitzes, M. Hammer-Wilson, L. H. Liaw, A. Siemens, M. Koonce, S. Peterson, S. Brenner, J. Burt, R. Walter, P. J. Bryant, D. van Dyk, J. Coulombe, T. Cahill, and G. S. Berns, "Laser microsurgery in cell and developmental biology," Science 213, 505-513 (1981).
[CrossRef] [PubMed]

Kumar, S.

S. Kumar, I. Z. Maxwell, A. Heisterkamp, T. R. Polte, T. P. Lele, M. Salanga, E. Mazur, and D. E. Ingber, "Viscoelastic retraction of single living stress fibers and its impact on cell shape, cytoskeletal organization, and extracellular matrix mechanics," Biophys. J. 90, 3762-3773 (2006).
[CrossRef] [PubMed]

Leischner, U.

H. Dodt, U. Leischner, A. Schierloh, N. Jährling, C. P. Mauch, K. Deininger, J. M. Deussing, M. Eder, W. Zieglgänsberger, and K. Becker, "Ultramicroscopy: three-dimensional visualization of neuronal networks in the whole mouse brain," Nat. Methods 4, 331-336 (2007).
[CrossRef] [PubMed]

Lele, T. P.

S. Kumar, I. Z. Maxwell, A. Heisterkamp, T. R. Polte, T. P. Lele, M. Salanga, E. Mazur, and D. E. Ingber, "Viscoelastic retraction of single living stress fibers and its impact on cell shape, cytoskeletal organization, and extracellular matrix mechanics," Biophys. J. 90, 3762-3773 (2006).
[CrossRef] [PubMed]

Liaw, L. H.

E. L. Botvinick, V. Venugopalan, J. V. Shah, L. H. Liaw, and M. W. Berns, "Controlled ablation of microtubules using a picosecond laser," Biophys. J. 87, 4203-4212 (2004).
[CrossRef] [PubMed]

M. W. Berns, J. Aist, J. Edwards, K. Strahs, J. Girton, P. McNeill, J. B. Rattner, M. Kitzes, M. Hammer-Wilson, L. H. Liaw, A. Siemens, M. Koonce, S. Peterson, S. Brenner, J. Burt, R. Walter, P. J. Bryant, D. van Dyk, J. Coulombe, T. Cahill, and G. S. Berns, "Laser microsurgery in cell and developmental biology," Science 213, 505-513 (1981).
[CrossRef] [PubMed]

Marcello, M.

P. J. Verveer, J. Swoger, F. Pampaloni, K. Greger, M. Marcello, and E.H.K. Stelzer, "High-resolution three-dimensional imaging of large specimens with light sheet-based microscopy," Nat. Methods 4, 311-313 (2007).
[PubMed]

Martin, J.

W. Supatto, D. Débarre, B. Moulia, E. Brouzés, J. Martin, E. Farge, and E. Beaurepaire, "In vivo modulation of morphogenetic movements in Drosophila embryos with femtosecond laser pulses," Proc. Natl. Acad. Sci. U S A 102, 1047-1052 (2005).
[CrossRef] [PubMed]

Martin, P.

B. Stramer, W. Wood, M. J. Galko, M. J. Redd, A. Jacinto, S. M. Parkhurst, and P. Martin, "Live imaging of wound inflammation in Drosophila embryos reveals key roles for small GTPases during in vivo cell migration," J. Cell Biol. 168, 567-573 (2005).
[CrossRef] [PubMed]

Mauch, C. P.

H. Dodt, U. Leischner, A. Schierloh, N. Jährling, C. P. Mauch, K. Deininger, J. M. Deussing, M. Eder, W. Zieglgänsberger, and K. Becker, "Ultramicroscopy: three-dimensional visualization of neuronal networks in the whole mouse brain," Nat. Methods 4, 331-336 (2007).
[CrossRef] [PubMed]

Maxwell, I. Z.

S. Kumar, I. Z. Maxwell, A. Heisterkamp, T. R. Polte, T. P. Lele, M. Salanga, E. Mazur, and D. E. Ingber, "Viscoelastic retraction of single living stress fibers and its impact on cell shape, cytoskeletal organization, and extracellular matrix mechanics," Biophys. J. 90, 3762-3773 (2006).
[CrossRef] [PubMed]

Mazur, E.

S. Kumar, I. Z. Maxwell, A. Heisterkamp, T. R. Polte, T. P. Lele, M. Salanga, E. Mazur, and D. E. Ingber, "Viscoelastic retraction of single living stress fibers and its impact on cell shape, cytoskeletal organization, and extracellular matrix mechanics," Biophys. J. 90, 3762-3773 (2006).
[CrossRef] [PubMed]

McNeill, P.

M. W. Berns, J. Aist, J. Edwards, K. Strahs, J. Girton, P. McNeill, J. B. Rattner, M. Kitzes, M. Hammer-Wilson, L. H. Liaw, A. Siemens, M. Koonce, S. Peterson, S. Brenner, J. Burt, R. Walter, P. J. Bryant, D. van Dyk, J. Coulombe, T. Cahill, and G. S. Berns, "Laser microsurgery in cell and developmental biology," Science 213, 505-513 (1981).
[CrossRef] [PubMed]

Montague, R. A.

D. P. Kiehart, C. G. Galbraith, K. A. Edwards, W. L. Rickoll, and R. A. Montague, "Multiple forces contribute to cell sheet morphogenesis for dorsal closure in Drosophila," J. Cell Biol. 149, 471-490 (2000).
[CrossRef] [PubMed]

Mostov, K. E.

L. E. O'Brien, M. M. P. Zegers, and K. E. Mostov, "Opinion: Building epithelial architecture: insights from three-dimensional culture models," Nat. Rev. Mol. Cell Biol. 3, 531-537 (2002).
[CrossRef]

Moulia, B.

W. Supatto, D. Débarre, B. Moulia, E. Brouzés, J. Martin, E. Farge, and E. Beaurepaire, "In vivo modulation of morphogenetic movements in Drosophila embryos with femtosecond laser pulses," Proc. Natl. Acad. Sci. U S A 102, 1047-1052 (2005).
[CrossRef] [PubMed]

Nimmerjahn, A.

A. Nimmerjahn, F. Kirchhoff, and F. Helmchen, "Resting microglial cells are highly dynamic surveillants of brain parenchyma in vivo," Science 308, 1314-1318 (2005).
[CrossRef] [PubMed]

O'Brien, L. E.

L. E. O'Brien, M. M. P. Zegers, and K. E. Mostov, "Opinion: Building epithelial architecture: insights from three-dimensional culture models," Nat. Rev. Mol. Cell Biol. 3, 531-537 (2002).
[CrossRef]

Pampaloni, F.

P. J. Verveer, J. Swoger, F. Pampaloni, K. Greger, M. Marcello, and E.H.K. Stelzer, "High-resolution three-dimensional imaging of large specimens with light sheet-based microscopy," Nat. Methods 4, 311-313 (2007).
[PubMed]

Parkhurst, S. M.

B. Stramer, W. Wood, M. J. Galko, M. J. Redd, A. Jacinto, S. M. Parkhurst, and P. Martin, "Live imaging of wound inflammation in Drosophila embryos reveals key roles for small GTPases during in vivo cell migration," J. Cell Biol. 168, 567-573 (2005).
[CrossRef] [PubMed]

Pepperkok, R.

J. Colombelli, E. G. Reynaud, J. Rietdorf, R. Pepperkok, and E. H. K. Stelzer, "In vivo selective cytoskeleton dynamics quantification in interphase cells induced by pulsed ultraviolet laser nanosurgery," Traffic 6, 1093-1102 (2005).
[CrossRef] [PubMed]

Peterson, S.

M. W. Berns, J. Aist, J. Edwards, K. Strahs, J. Girton, P. McNeill, J. B. Rattner, M. Kitzes, M. Hammer-Wilson, L. H. Liaw, A. Siemens, M. Koonce, S. Peterson, S. Brenner, J. Burt, R. Walter, P. J. Bryant, D. van Dyk, J. Coulombe, T. Cahill, and G. S. Berns, "Laser microsurgery in cell and developmental biology," Science 213, 505-513 (1981).
[CrossRef] [PubMed]

Polte, T. R.

S. Kumar, I. Z. Maxwell, A. Heisterkamp, T. R. Polte, T. P. Lele, M. Salanga, E. Mazur, and D. E. Ingber, "Viscoelastic retraction of single living stress fibers and its impact on cell shape, cytoskeletal organization, and extracellular matrix mechanics," Biophys. J. 90, 3762-3773 (2006).
[CrossRef] [PubMed]

Rattner, J. B.

M. W. Berns, J. Aist, J. Edwards, K. Strahs, J. Girton, P. McNeill, J. B. Rattner, M. Kitzes, M. Hammer-Wilson, L. H. Liaw, A. Siemens, M. Koonce, S. Peterson, S. Brenner, J. Burt, R. Walter, P. J. Bryant, D. van Dyk, J. Coulombe, T. Cahill, and G. S. Berns, "Laser microsurgery in cell and developmental biology," Science 213, 505-513 (1981).
[CrossRef] [PubMed]

Redd, M. J.

B. Stramer, W. Wood, M. J. Galko, M. J. Redd, A. Jacinto, S. M. Parkhurst, and P. Martin, "Live imaging of wound inflammation in Drosophila embryos reveals key roles for small GTPases during in vivo cell migration," J. Cell Biol. 168, 567-573 (2005).
[CrossRef] [PubMed]

Reynaud, E. G.

J. Colombelli, E. G. Reynaud, J. Rietdorf, R. Pepperkok, and E. H. K. Stelzer, "In vivo selective cytoskeleton dynamics quantification in interphase cells induced by pulsed ultraviolet laser nanosurgery," Traffic 6, 1093-1102 (2005).
[CrossRef] [PubMed]

Rickoll, W. L.

D. P. Kiehart, C. G. Galbraith, K. A. Edwards, W. L. Rickoll, and R. A. Montague, "Multiple forces contribute to cell sheet morphogenesis for dorsal closure in Drosophila," J. Cell Biol. 149, 471-490 (2000).
[CrossRef] [PubMed]

Rietdorf, J.

J. Colombelli, E. G. Reynaud, J. Rietdorf, R. Pepperkok, and E. H. K. Stelzer, "In vivo selective cytoskeleton dynamics quantification in interphase cells induced by pulsed ultraviolet laser nanosurgery," Traffic 6, 1093-1102 (2005).
[CrossRef] [PubMed]

Salanga, M.

S. Kumar, I. Z. Maxwell, A. Heisterkamp, T. R. Polte, T. P. Lele, M. Salanga, E. Mazur, and D. E. Ingber, "Viscoelastic retraction of single living stress fibers and its impact on cell shape, cytoskeletal organization, and extracellular matrix mechanics," Biophys. J. 90, 3762-3773 (2006).
[CrossRef] [PubMed]

Schierloh, A.

H. Dodt, U. Leischner, A. Schierloh, N. Jährling, C. P. Mauch, K. Deininger, J. M. Deussing, M. Eder, W. Zieglgänsberger, and K. Becker, "Ultramicroscopy: three-dimensional visualization of neuronal networks in the whole mouse brain," Nat. Methods 4, 331-336 (2007).
[CrossRef] [PubMed]

Shah, J. V.

E. L. Botvinick, V. Venugopalan, J. V. Shah, L. H. Liaw, and M. W. Berns, "Controlled ablation of microtubules using a picosecond laser," Biophys. J. 87, 4203-4212 (2004).
[CrossRef] [PubMed]

Siemens, A.

M. W. Berns, J. Aist, J. Edwards, K. Strahs, J. Girton, P. McNeill, J. B. Rattner, M. Kitzes, M. Hammer-Wilson, L. H. Liaw, A. Siemens, M. Koonce, S. Peterson, S. Brenner, J. Burt, R. Walter, P. J. Bryant, D. van Dyk, J. Coulombe, T. Cahill, and G. S. Berns, "Laser microsurgery in cell and developmental biology," Science 213, 505-513 (1981).
[CrossRef] [PubMed]

Stelzer, E. H. K.

K. Greger, J. Swoger, and E. H. K. Stelzer, "Basic building units and properties of a fluorescence Single Plane Illumination Microscope (SPIM)," Rev. Sci. Instrum. 78, 023705 (2007).
[CrossRef] [PubMed]

C. J. Engelbrecht, and E. H. K. Stelzer, "Resolution enhancement in a light-sheet-based microscope (SPIM)," Opt. Lett. 31, 1477-1479 (2006).
[CrossRef] [PubMed]

J. Colombelli, E. G. Reynaud, J. Rietdorf, R. Pepperkok, and E. H. K. Stelzer, "In vivo selective cytoskeleton dynamics quantification in interphase cells induced by pulsed ultraviolet laser nanosurgery," Traffic 6, 1093-1102 (2005).
[CrossRef] [PubMed]

J. Colombelli, S. W. Grill, and E. H. K. Stelzer, "UV diffraction limited nanosurgery of live biological tissues," Rev. Sci. Instrum. 75, 472-478 (2004).
[CrossRef]

J. Huisken, J. Swoger, F. D. Bene, J. Wittbrodt, and E. H. K. Stelzer, "Optical sectioning deep inside live embryos by selective plane illumination microscopy," Science 305, 1007-1009 (2004).
[CrossRef] [PubMed]

S. W. Grill, P. Gönczy, E. H. K. Stelzer, and A. A. Hyman, "Polarity controls forces governing asymmetric spindle positioning in the Caenorhabditis elegans embryo," Nature 409, 630-633 (2001).
[CrossRef] [PubMed]

Stelzer, E.H. K.

Stelzer, E.H.K.

P. J. Verveer, J. Swoger, F. Pampaloni, K. Greger, M. Marcello, and E.H.K. Stelzer, "High-resolution three-dimensional imaging of large specimens with light sheet-based microscopy," Nat. Methods 4, 311-313 (2007).
[PubMed]

Strahs, K.

M. W. Berns, J. Aist, J. Edwards, K. Strahs, J. Girton, P. McNeill, J. B. Rattner, M. Kitzes, M. Hammer-Wilson, L. H. Liaw, A. Siemens, M. Koonce, S. Peterson, S. Brenner, J. Burt, R. Walter, P. J. Bryant, D. van Dyk, J. Coulombe, T. Cahill, and G. S. Berns, "Laser microsurgery in cell and developmental biology," Science 213, 505-513 (1981).
[CrossRef] [PubMed]

Stramer, B.

B. Stramer, W. Wood, M. J. Galko, M. J. Redd, A. Jacinto, S. M. Parkhurst, and P. Martin, "Live imaging of wound inflammation in Drosophila embryos reveals key roles for small GTPases during in vivo cell migration," J. Cell Biol. 168, 567-573 (2005).
[CrossRef] [PubMed]

Supatto, W.

W. Supatto, D. Débarre, B. Moulia, E. Brouzés, J. Martin, E. Farge, and E. Beaurepaire, "In vivo modulation of morphogenetic movements in Drosophila embryos with femtosecond laser pulses," Proc. Natl. Acad. Sci. U S A 102, 1047-1052 (2005).
[CrossRef] [PubMed]

Swartz, M. A.

L. G. Griffith, and M. A. Swartz, "Capturing complex 3D tissue physiology in vitro," Nat. Rev. Mol. Cell Biol. 7, 211-224 (2006).
[CrossRef] [PubMed]

Swoger, J.

K. Greger, J. Swoger, and E. H. K. Stelzer, "Basic building units and properties of a fluorescence Single Plane Illumination Microscope (SPIM)," Rev. Sci. Instrum. 78, 023705 (2007).
[CrossRef] [PubMed]

P. J. Verveer, J. Swoger, F. Pampaloni, K. Greger, M. Marcello, and E.H.K. Stelzer, "High-resolution three-dimensional imaging of large specimens with light sheet-based microscopy," Nat. Methods 4, 311-313 (2007).
[PubMed]

J. Huisken, J. Swoger, F. D. Bene, J. Wittbrodt, and E. H. K. Stelzer, "Optical sectioning deep inside live embryos by selective plane illumination microscopy," Science 305, 1007-1009 (2004).
[CrossRef] [PubMed]

Tokutake, Y.

M. S. Hutson, Y. Tokutake, M. Chang, J. W. Bloor, S. Venakides, D. P. Kiehart, and G. S. Edwards, "Forces for morphogenesis investigated with laser microsurgery and quantitative modeling," Science 300, 145-149 (2003).
[CrossRef] [PubMed]

van Dyk, D.

M. W. Berns, J. Aist, J. Edwards, K. Strahs, J. Girton, P. McNeill, J. B. Rattner, M. Kitzes, M. Hammer-Wilson, L. H. Liaw, A. Siemens, M. Koonce, S. Peterson, S. Brenner, J. Burt, R. Walter, P. J. Bryant, D. van Dyk, J. Coulombe, T. Cahill, and G. S. Berns, "Laser microsurgery in cell and developmental biology," Science 213, 505-513 (1981).
[CrossRef] [PubMed]

Venakides, S.

M. S. Hutson, Y. Tokutake, M. Chang, J. W. Bloor, S. Venakides, D. P. Kiehart, and G. S. Edwards, "Forces for morphogenesis investigated with laser microsurgery and quantitative modeling," Science 300, 145-149 (2003).
[CrossRef] [PubMed]

Venugopalan, V.

E. L. Botvinick, V. Venugopalan, J. V. Shah, L. H. Liaw, and M. W. Berns, "Controlled ablation of microtubules using a picosecond laser," Biophys. J. 87, 4203-4212 (2004).
[CrossRef] [PubMed]

A. Vogel, and V. Venugopalan, "Mechanisms of pulsed laser ablation of biological tissues," Chem. Rev. 103, 577-644 (2003).
[CrossRef] [PubMed]

Verveer, P. J.

P. J. Verveer, J. Swoger, F. Pampaloni, K. Greger, M. Marcello, and E.H.K. Stelzer, "High-resolution three-dimensional imaging of large specimens with light sheet-based microscopy," Nat. Methods 4, 311-313 (2007).
[PubMed]

Vogel, A.

A. Vogel, and V. Venugopalan, "Mechanisms of pulsed laser ablation of biological tissues," Chem. Rev. 103, 577-644 (2003).
[CrossRef] [PubMed]

Walter, R.

M. W. Berns, J. Aist, J. Edwards, K. Strahs, J. Girton, P. McNeill, J. B. Rattner, M. Kitzes, M. Hammer-Wilson, L. H. Liaw, A. Siemens, M. Koonce, S. Peterson, S. Brenner, J. Burt, R. Walter, P. J. Bryant, D. van Dyk, J. Coulombe, T. Cahill, and G. S. Berns, "Laser microsurgery in cell and developmental biology," Science 213, 505-513 (1981).
[CrossRef] [PubMed]

Wittbrodt, J.

J. Huisken, J. Swoger, F. D. Bene, J. Wittbrodt, and E. H. K. Stelzer, "Optical sectioning deep inside live embryos by selective plane illumination microscopy," Science 305, 1007-1009 (2004).
[CrossRef] [PubMed]

Wood, W.

B. Stramer, W. Wood, M. J. Galko, M. J. Redd, A. Jacinto, S. M. Parkhurst, and P. Martin, "Live imaging of wound inflammation in Drosophila embryos reveals key roles for small GTPases during in vivo cell migration," J. Cell Biol. 168, 567-573 (2005).
[CrossRef] [PubMed]

Zegers, M. M. P.

L. E. O'Brien, M. M. P. Zegers, and K. E. Mostov, "Opinion: Building epithelial architecture: insights from three-dimensional culture models," Nat. Rev. Mol. Cell Biol. 3, 531-537 (2002).
[CrossRef]

Zieglgänsberger, W.

H. Dodt, U. Leischner, A. Schierloh, N. Jährling, C. P. Mauch, K. Deininger, J. M. Deussing, M. Eder, W. Zieglgänsberger, and K. Becker, "Ultramicroscopy: three-dimensional visualization of neuronal networks in the whole mouse brain," Nat. Methods 4, 331-336 (2007).
[CrossRef] [PubMed]

Biophys. J. (2)

E. L. Botvinick, V. Venugopalan, J. V. Shah, L. H. Liaw, and M. W. Berns, "Controlled ablation of microtubules using a picosecond laser," Biophys. J. 87, 4203-4212 (2004).
[CrossRef] [PubMed]

S. Kumar, I. Z. Maxwell, A. Heisterkamp, T. R. Polte, T. P. Lele, M. Salanga, E. Mazur, and D. E. Ingber, "Viscoelastic retraction of single living stress fibers and its impact on cell shape, cytoskeletal organization, and extracellular matrix mechanics," Biophys. J. 90, 3762-3773 (2006).
[CrossRef] [PubMed]

Chem. Rev. (1)

A. Vogel, and V. Venugopalan, "Mechanisms of pulsed laser ablation of biological tissues," Chem. Rev. 103, 577-644 (2003).
[CrossRef] [PubMed]

Dev. Cell (1)

F. Jankovics, and D. Brunner, "Transiently reorganized microtubules are essential for zippering during dorsal closure in Drosophila melanogaster," Dev. Cell 11, 375-385 (2006).
[CrossRef] [PubMed]

Exp. Neurol. (1)

D. B. Edelman and E. W. Keefer, "A cultural renaissance: in vitro cell biology embraces three-dimensional context," Exp. Neurol. 192, 1-6 (2005).
[CrossRef] [PubMed]

J. Cell Biol. (2)

D. P. Kiehart, C. G. Galbraith, K. A. Edwards, W. L. Rickoll, and R. A. Montague, "Multiple forces contribute to cell sheet morphogenesis for dorsal closure in Drosophila," J. Cell Biol. 149, 471-490 (2000).
[CrossRef] [PubMed]

B. Stramer, W. Wood, M. J. Galko, M. J. Redd, A. Jacinto, S. M. Parkhurst, and P. Martin, "Live imaging of wound inflammation in Drosophila embryos reveals key roles for small GTPases during in vivo cell migration," J. Cell Biol. 168, 567-573 (2005).
[CrossRef] [PubMed]

J. Cell Sci. (1)

R. Farooqui, and G. Fenteany, "Multiple rows of cells behind an epithelial wound edge extend cryptic lamellipodia to collectively drive cell-sheet movement," J. Cell Sci. 118, 51-63 (2005).
[CrossRef]

J. Opt. Soc. Am. A (1)

Nat. Methods (2)

P. J. Verveer, J. Swoger, F. Pampaloni, K. Greger, M. Marcello, and E.H.K. Stelzer, "High-resolution three-dimensional imaging of large specimens with light sheet-based microscopy," Nat. Methods 4, 311-313 (2007).
[PubMed]

H. Dodt, U. Leischner, A. Schierloh, N. Jährling, C. P. Mauch, K. Deininger, J. M. Deussing, M. Eder, W. Zieglgänsberger, and K. Becker, "Ultramicroscopy: three-dimensional visualization of neuronal networks in the whole mouse brain," Nat. Methods 4, 331-336 (2007).
[CrossRef] [PubMed]

Nat. Rev. Mol. Cell Biol. (2)

L. E. O'Brien, M. M. P. Zegers, and K. E. Mostov, "Opinion: Building epithelial architecture: insights from three-dimensional culture models," Nat. Rev. Mol. Cell Biol. 3, 531-537 (2002).
[CrossRef]

L. G. Griffith, and M. A. Swartz, "Capturing complex 3D tissue physiology in vitro," Nat. Rev. Mol. Cell Biol. 7, 211-224 (2006).
[CrossRef] [PubMed]

Nature (1)

S. W. Grill, P. Gönczy, E. H. K. Stelzer, and A. A. Hyman, "Polarity controls forces governing asymmetric spindle positioning in the Caenorhabditis elegans embryo," Nature 409, 630-633 (2001).
[CrossRef] [PubMed]

Opt. Lett. (1)

Proc. Natl. Acad. Sci. U S A (1)

W. Supatto, D. Débarre, B. Moulia, E. Brouzés, J. Martin, E. Farge, and E. Beaurepaire, "In vivo modulation of morphogenetic movements in Drosophila embryos with femtosecond laser pulses," Proc. Natl. Acad. Sci. U S A 102, 1047-1052 (2005).
[CrossRef] [PubMed]

Rev. Sci. Instrum. (2)

K. Greger, J. Swoger, and E. H. K. Stelzer, "Basic building units and properties of a fluorescence Single Plane Illumination Microscope (SPIM)," Rev. Sci. Instrum. 78, 023705 (2007).
[CrossRef] [PubMed]

J. Colombelli, S. W. Grill, and E. H. K. Stelzer, "UV diffraction limited nanosurgery of live biological tissues," Rev. Sci. Instrum. 75, 472-478 (2004).
[CrossRef]

Science (4)

J. Huisken, J. Swoger, F. D. Bene, J. Wittbrodt, and E. H. K. Stelzer, "Optical sectioning deep inside live embryos by selective plane illumination microscopy," Science 305, 1007-1009 (2004).
[CrossRef] [PubMed]

M. W. Berns, J. Aist, J. Edwards, K. Strahs, J. Girton, P. McNeill, J. B. Rattner, M. Kitzes, M. Hammer-Wilson, L. H. Liaw, A. Siemens, M. Koonce, S. Peterson, S. Brenner, J. Burt, R. Walter, P. J. Bryant, D. van Dyk, J. Coulombe, T. Cahill, and G. S. Berns, "Laser microsurgery in cell and developmental biology," Science 213, 505-513 (1981).
[CrossRef] [PubMed]

M. S. Hutson, Y. Tokutake, M. Chang, J. W. Bloor, S. Venakides, D. P. Kiehart, and G. S. Edwards, "Forces for morphogenesis investigated with laser microsurgery and quantitative modeling," Science 300, 145-149 (2003).
[CrossRef] [PubMed]

A. Nimmerjahn, F. Kirchhoff, and F. Helmchen, "Resting microglial cells are highly dynamic surveillants of brain parenchyma in vivo," Science 308, 1314-1318 (2005).
[CrossRef] [PubMed]

Traffic (1)

J. Colombelli, E. G. Reynaud, J. Rietdorf, R. Pepperkok, and E. H. K. Stelzer, "In vivo selective cytoskeleton dynamics quantification in interphase cells induced by pulsed ultraviolet laser nanosurgery," Traffic 6, 1093-1102 (2005).
[CrossRef] [PubMed]

Supplementary Material (2)

» Media 1: MOV (799 KB)     
» Media 2: MOV (592 KB)     

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Figures (7)

Fig. 1.
Fig. 1.

SPIM laser cutter optical setup: (a) schematic and (b) perspective representations. Pulses (blue lines) from the UV-Laser UVL are intensity-adjusted by an acousto-optic modulator AOM and deflected by a mirror M. Only the 1st diffracted order from the AOM passes the irises I1 and I2 and is expanded by a beam expander BE. Subsequently, the x/y beam steering device BSD rotates the beam around its principle point. The scan lens SL and tube lens TL1 image this rotation into the back focal plane of the detection objective lens OL. Thus, the UV-focus is scanned across the specimen S in the sample chamber SC. For fluorescence excitation, a collimated visible laser beam (green bars) is focused by a cylindrical lens CL, which generates a light sheet. The numerical aperture and the height (along the x-axis) are adjusted by a rectangular diaphragm RD. The light sheet excites only fluorescent molecules in the focal plane of the detection objective lens, since the illumination axis is rotated by an angle θ = 90° relative to the detection axis. The specimen can be translated along three principal axes x, y, z and rotated about an angle φ. Three-dimensional image stacks are acquired by moving the specimen along the z-axis; all optical parts are stationary. A conventional widefield microscope setup (OL, emission filter EL, tube lens TL2, and camera CCD) is used for fluorescence detection.

Fig. 2.
Fig. 2.

Dissection of single microtubules. Fluorescence images of Cy3-labeled, Taxol-stabilized microtubules are provided before (a) and 2 seconds after (b) dissection. Shearing as well as bleached areas (yellow arrows) are visible after irradiation. The blue line was scanned by 50 UV-pulses with energies of 40 ± 5 nJ at a pulse repetition rate of 1 kHz and a scan step width of 0.1 μm. Dissection and imaging were performed through a Zeiss Achroplan 100x/1.0W objective lens. A fluorescence excitation wavelength of λexc = 514 nm and a Razoredge 514 LP emission filter were used. Scale bars are 10 μm.

Fig. 3.
Fig. 3.

Dissection of single microtubules in three dimensions. Perspective representations (volume rendering) of Cy3-labeled, Taxol-stabilized microtubules are provided before (a) and 10 seconds after dissection (b). The data stacks are parts of a time lapse consisting of 24 time points acquired over a period of 4 minutes. Cutting and imaging parameters were identical to those in Fig. 2, except that the blue line was scanned by 75 UV-pulses instead of 50. Projected raster lines in the background have a spacing of 5 μm.

Fig. 4.
Fig. 4.

Targeted membrane breakdown of MDCK cysts. The cells expressed a GFP-actin construct. The left column provides single section planes (~ 14 μm inside the cyst, roughly 1/3 of the cyst diameter, plane of ablation) from fluorescence datasets, whereas the right column provides perspective representations (volume rendering) of the whole cyst. The yellow plane indicates the position of the section planes in the left column. Membranes of single cells were irradiated on two points at their outer edge (blue arrows). Energies of 0.25 ± 0.03 μJ and a repetition rate of 100 Hz were used to focus 20 UV pulses onto each target through a Zeiss Achroplan 63x/0.9W objective lens. The green signal (λexc = 488 nm, Razoredge 488 LP emission filter, signal subtraction) corresponds to the GFP signal, whereas the red channel (λexc = 543 nm, 610/75 band pass emission filter) provides the PI distribution. The datasets shown in rows a, b, and c were recorded right before, directly after, and 5 minutes after laser ablation, respectively. Scale bars are 20 μm.

Fig. 5.
Fig. 5.

Three-dimensional ablation of a fixed Zebrafish caudal fin. The images in the left column are recorded in transmission, whereas the right column provides perspective isosurface renderings of fluorescence datasets (excitation wavelength λexc = 488 nm, emission filter Razoredge 488 LP). The cells are fluorescently labeled with 1 mM DioC6. Cutting and imaging were performed with a Zeiss Achroplan 40x/0.8W objective lens. The blue x/z planes have dimensions of 82.5 × 50.0 μm, 72.0 × 50.0 μm, and 30.0 × 50.0 μm respectively, and indicate the dissected areas. Every μm2 of these cutting planes was irradiated with 15 UV pulses with energies of 0.57 ± 0.03 μJ at a repetition rate of 800 Hz. The yellow arrows in row (c) point to a region in the vicinity of the ablated planes (a) and (b), where fluorescent molecules were bleached by the UV pulses. Scale bars are 50 μm.

Fig. 6.
Fig. 6.

Laser-induced hemocyte migration in stage 15 Drosophila embryo: Maximum projections through a stack of fluorescence images before (a) and after (b) laser wounding are provided. Hemocyte trajectories over time are indicated by the red traces in figure (b). The data has been recorded with a Zeiss Achroplan 40x/0.8W objective lens, an excitation wavelength of λexc = 488 nm, and an emission filter Razoredge 488 LP. GFP is selectively expressed in hemocytes. At t=0 min, the indicated area (filled blue circle) was irradiated with 100 UV-pulses with pulse energies of 0.74±0.04 μJ at a repetition rate of 1 kHz. Supplementary Movies 1 and 2 demonstrate the migration of hemocytes over a time of 29 mins after irradiation. The scale bar is 50 μm. [Media 1] [Media 2]

Fig. 7.
Fig. 7.

Sample mounting on the microscope-microscalpel system. In the left panel, the approach used with the cysts and MTs is illustrated. Liquid containing the biological samples of interest is filled into a heat-sealed cylinder consisting of BioFoil (In Vitro Systems & Services, Germany). In the right panel, the mounting scheme used in the Zebrafish-experiment is illustrated. After dissection and staining, the fin is clipped in a syringe and mounted on the microsope for imaging and laser ablation.

Tables (2)

Tables Icon

Table 1: Measured and calculated values for lateral and axial extents of the UV-ablated volumes

Tables Icon

Table 2: Ablation parameters used in the different experiments

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