Abstract

We used a fiber-optical dual-beam trap (single-mode fiber, λ = 532nm, trapping power ∼ 22mW, the distance between the two fiber end-faces = 125μm) to capture a Chinese hamster ovary (CHO) cell with a diameter of approximately 15μm and tracked its three-dimensional Brownian motion via a pair of orthogonal quadrant photodiodes. By analyzing the Brownian motion of the trapped CHO cell, we determined the force constants of the optical force field on the CHO cell to be kx=6.75 pN/μm, ky=5.53 pN/μm, kz=1.96 pN/μm, and kx=2.91 pN/μm, ky=2.7 pN/μm, kz=0.79 pN/μm, respectively, before and after the CHO cell was treated with latrunculin, a toxic drug known to disrupt the cytoskeleton of the cell.

© 2006 Optical Society of America

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References

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    [CrossRef] [PubMed]
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    [CrossRef]
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    [CrossRef] [PubMed]
  14. J. Guck, S. Schinkinger, B. Lincoln, F. Wottawah, S. Ebert, M. Romeyke, D. Lenz, H. M. Erickson, R. Ananthakrishnan, D. Mitchell, J. Kas, S. Ulvick, and C. Bilby, "Optical deformability as an inherent cell marker for testing malignant transformation and metastatic competence," Biophys. J. 88, 3689-3698 (2005).

2005

2002

N. Malagnino, G. Pesce, A. Sasso, and E. Arimondo, "Measurements of trapping efficiency and stiffness inoptical tweezers," Opt. Commun. 214, 15-24 (2002).
[CrossRef]

2000

J. Guck, R. Ananthakrishnan, T. J. Moon, C. C. Cunningham, and J. Kas, "Optical deformability of soft biological dielectrics," Phys. Rev. Lett. 84, 5451-5454 (2000).
[CrossRef] [PubMed]

1998

E.-L. Florin, A. Pralle, E. H. K. Stelzer, and J. K. H. Horber, "Photonic forcemicroscope calibration by thermal noise analysis," Appl. Phys. A 66, 75-78 (1998).
[CrossRef]

1997

1993

1991

S. C. Erzurum, M. L. Kus, C. Bohse, E. L. Elson, and G. S. Worthen, "Mechanical properties of HL60 cells: role of stimulation and differentiation in retention in capillary-sized pores," Am. J. Respir. Cell Mol Biol. 5, 230-241 (1991).
[CrossRef] [PubMed]

1986

1970

A. Ashkin, "Acceleration and trapping of particles by radiation pressure," Phys. Rev. Lett. 24, 156-159 (1970).
[CrossRef]

Ananthakrishnan, R.

J. Guck, S. Schinkinger, B. Lincoln, F. Wottawah, S. Ebert, M. Romeyke, D. Lenz, H. M. Erickson, R. Ananthakrishnan, D. Mitchell, J. Kas, S. Ulvick, and C. Bilby, "Optical deformability as an inherent cell marker for testing malignant transformation and metastatic competence," Biophys. J. 88, 3689-3698 (2005).

J. Guck, R. Ananthakrishnan, T. J. Moon, C. C. Cunningham, and J. Kas, "Optical deformability of soft biological dielectrics," Phys. Rev. Lett. 84, 5451-5454 (2000).
[CrossRef] [PubMed]

Arimondo, E.

N. Malagnino, G. Pesce, A. Sasso, and E. Arimondo, "Measurements of trapping efficiency and stiffness inoptical tweezers," Opt. Commun. 214, 15-24 (2002).
[CrossRef]

Ashkin, A.

Bilby, C.

J. Guck, S. Schinkinger, B. Lincoln, F. Wottawah, S. Ebert, M. Romeyke, D. Lenz, H. M. Erickson, R. Ananthakrishnan, D. Mitchell, J. Kas, S. Ulvick, and C. Bilby, "Optical deformability as an inherent cell marker for testing malignant transformation and metastatic competence," Biophys. J. 88, 3689-3698 (2005).

Bjorkholm, J.

Bohse, C.

S. C. Erzurum, M. L. Kus, C. Bohse, E. L. Elson, and G. S. Worthen, "Mechanical properties of HL60 cells: role of stimulation and differentiation in retention in capillary-sized pores," Am. J. Respir. Cell Mol Biol. 5, 230-241 (1991).
[CrossRef] [PubMed]

Chiou, A.

Chu, S.

Collins, S. D.

Constable, A.

Cunningham, C. C.

J. Guck, R. Ananthakrishnan, T. J. Moon, C. C. Cunningham, and J. Kas, "Optical deformability of soft biological dielectrics," Phys. Rev. Lett. 84, 5451-5454 (2000).
[CrossRef] [PubMed]

Dziedzic, J.

Ebert, S.

J. Guck, S. Schinkinger, B. Lincoln, F. Wottawah, S. Ebert, M. Romeyke, D. Lenz, H. M. Erickson, R. Ananthakrishnan, D. Mitchell, J. Kas, S. Ulvick, and C. Bilby, "Optical deformability as an inherent cell marker for testing malignant transformation and metastatic competence," Biophys. J. 88, 3689-3698 (2005).

Elson, E. L.

S. C. Erzurum, M. L. Kus, C. Bohse, E. L. Elson, and G. S. Worthen, "Mechanical properties of HL60 cells: role of stimulation and differentiation in retention in capillary-sized pores," Am. J. Respir. Cell Mol Biol. 5, 230-241 (1991).
[CrossRef] [PubMed]

Erickson, H. M.

J. Guck, S. Schinkinger, B. Lincoln, F. Wottawah, S. Ebert, M. Romeyke, D. Lenz, H. M. Erickson, R. Ananthakrishnan, D. Mitchell, J. Kas, S. Ulvick, and C. Bilby, "Optical deformability as an inherent cell marker for testing malignant transformation and metastatic competence," Biophys. J. 88, 3689-3698 (2005).

Erzurum, S. C.

S. C. Erzurum, M. L. Kus, C. Bohse, E. L. Elson, and G. S. Worthen, "Mechanical properties of HL60 cells: role of stimulation and differentiation in retention in capillary-sized pores," Am. J. Respir. Cell Mol Biol. 5, 230-241 (1991).
[CrossRef] [PubMed]

Florin, E.-L.

E.-L. Florin, A. Pralle, E. H. K. Stelzer, and J. K. H. Horber, "Photonic forcemicroscope calibration by thermal noise analysis," Appl. Phys. A 66, 75-78 (1998).
[CrossRef]

Ghislain, L. P.

Guck, J.

J. Guck, S. Schinkinger, B. Lincoln, F. Wottawah, S. Ebert, M. Romeyke, D. Lenz, H. M. Erickson, R. Ananthakrishnan, D. Mitchell, J. Kas, S. Ulvick, and C. Bilby, "Optical deformability as an inherent cell marker for testing malignant transformation and metastatic competence," Biophys. J. 88, 3689-3698 (2005).

J. Guck, R. Ananthakrishnan, T. J. Moon, C. C. Cunningham, and J. Kas, "Optical deformability of soft biological dielectrics," Phys. Rev. Lett. 84, 5451-5454 (2000).
[CrossRef] [PubMed]

Horber, J. K. H.

E.-L. Florin, A. Pralle, E. H. K. Stelzer, and J. K. H. Horber, "Photonic forcemicroscope calibration by thermal noise analysis," Appl. Phys. A 66, 75-78 (1998).
[CrossRef]

Jensen-McMullin, C.

Kas, J.

J. Guck, S. Schinkinger, B. Lincoln, F. Wottawah, S. Ebert, M. Romeyke, D. Lenz, H. M. Erickson, R. Ananthakrishnan, D. Mitchell, J. Kas, S. Ulvick, and C. Bilby, "Optical deformability as an inherent cell marker for testing malignant transformation and metastatic competence," Biophys. J. 88, 3689-3698 (2005).

J. Guck, R. Ananthakrishnan, T. J. Moon, C. C. Cunningham, and J. Kas, "Optical deformability of soft biological dielectrics," Phys. Rev. Lett. 84, 5451-5454 (2000).
[CrossRef] [PubMed]

Kim, J.

Knoesen, A.

Kus, M. L.

S. C. Erzurum, M. L. Kus, C. Bohse, E. L. Elson, and G. S. Worthen, "Mechanical properties of HL60 cells: role of stimulation and differentiation in retention in capillary-sized pores," Am. J. Respir. Cell Mol Biol. 5, 230-241 (1991).
[CrossRef] [PubMed]

Lee, H. P.

Lenz, D.

J. Guck, S. Schinkinger, B. Lincoln, F. Wottawah, S. Ebert, M. Romeyke, D. Lenz, H. M. Erickson, R. Ananthakrishnan, D. Mitchell, J. Kas, S. Ulvick, and C. Bilby, "Optical deformability as an inherent cell marker for testing malignant transformation and metastatic competence," Biophys. J. 88, 3689-3698 (2005).

Lincoln, B.

J. Guck, S. Schinkinger, B. Lincoln, F. Wottawah, S. Ebert, M. Romeyke, D. Lenz, H. M. Erickson, R. Ananthakrishnan, D. Mitchell, J. Kas, S. Ulvick, and C. Bilby, "Optical deformability as an inherent cell marker for testing malignant transformation and metastatic competence," Biophys. J. 88, 3689-3698 (2005).

Lyons, E. R.

Malagnino, N.

N. Malagnino, G. Pesce, A. Sasso, and E. Arimondo, "Measurements of trapping efficiency and stiffness inoptical tweezers," Opt. Commun. 214, 15-24 (2002).
[CrossRef]

Mervis, J.

Mitchell, D.

J. Guck, S. Schinkinger, B. Lincoln, F. Wottawah, S. Ebert, M. Romeyke, D. Lenz, H. M. Erickson, R. Ananthakrishnan, D. Mitchell, J. Kas, S. Ulvick, and C. Bilby, "Optical deformability as an inherent cell marker for testing malignant transformation and metastatic competence," Biophys. J. 88, 3689-3698 (2005).

Moon, T. J.

J. Guck, R. Ananthakrishnan, T. J. Moon, C. C. Cunningham, and J. Kas, "Optical deformability of soft biological dielectrics," Phys. Rev. Lett. 84, 5451-5454 (2000).
[CrossRef] [PubMed]

Pesce, G.

N. Malagnino, G. Pesce, A. Sasso, and E. Arimondo, "Measurements of trapping efficiency and stiffness inoptical tweezers," Opt. Commun. 214, 15-24 (2002).
[CrossRef]

Pralle, A.

E.-L. Florin, A. Pralle, E. H. K. Stelzer, and J. K. H. Horber, "Photonic forcemicroscope calibration by thermal noise analysis," Appl. Phys. A 66, 75-78 (1998).
[CrossRef]

Prentiss, M.

Romeyke, M.

J. Guck, S. Schinkinger, B. Lincoln, F. Wottawah, S. Ebert, M. Romeyke, D. Lenz, H. M. Erickson, R. Ananthakrishnan, D. Mitchell, J. Kas, S. Ulvick, and C. Bilby, "Optical deformability as an inherent cell marker for testing malignant transformation and metastatic competence," Biophys. J. 88, 3689-3698 (2005).

Sasso, A.

N. Malagnino, G. Pesce, A. Sasso, and E. Arimondo, "Measurements of trapping efficiency and stiffness inoptical tweezers," Opt. Commun. 214, 15-24 (2002).
[CrossRef]

Schinkinger, S.

J. Guck, S. Schinkinger, B. Lincoln, F. Wottawah, S. Ebert, M. Romeyke, D. Lenz, H. M. Erickson, R. Ananthakrishnan, D. Mitchell, J. Kas, S. Ulvick, and C. Bilby, "Optical deformability as an inherent cell marker for testing malignant transformation and metastatic competence," Biophys. J. 88, 3689-3698 (2005).

Sidick, E.

Stelzer, E. H. K.

E.-L. Florin, A. Pralle, E. H. K. Stelzer, and J. K. H. Horber, "Photonic forcemicroscope calibration by thermal noise analysis," Appl. Phys. A 66, 75-78 (1998).
[CrossRef]

Ulvick, S.

J. Guck, S. Schinkinger, B. Lincoln, F. Wottawah, S. Ebert, M. Romeyke, D. Lenz, H. M. Erickson, R. Ananthakrishnan, D. Mitchell, J. Kas, S. Ulvick, and C. Bilby, "Optical deformability as an inherent cell marker for testing malignant transformation and metastatic competence," Biophys. J. 88, 3689-3698 (2005).

Webb, W. W.

Wei, M.-T.

Worthen, G. S.

S. C. Erzurum, M. L. Kus, C. Bohse, E. L. Elson, and G. S. Worthen, "Mechanical properties of HL60 cells: role of stimulation and differentiation in retention in capillary-sized pores," Am. J. Respir. Cell Mol Biol. 5, 230-241 (1991).
[CrossRef] [PubMed]

Wottawah, F.

J. Guck, S. Schinkinger, B. Lincoln, F. Wottawah, S. Ebert, M. Romeyke, D. Lenz, H. M. Erickson, R. Ananthakrishnan, D. Mitchell, J. Kas, S. Ulvick, and C. Bilby, "Optical deformability as an inherent cell marker for testing malignant transformation and metastatic competence," Biophys. J. 88, 3689-3698 (2005).

Zarinetchi, F.

Am. J. Respir. Cell Mol Biol.

S. C. Erzurum, M. L. Kus, C. Bohse, E. L. Elson, and G. S. Worthen, "Mechanical properties of HL60 cells: role of stimulation and differentiation in retention in capillary-sized pores," Am. J. Respir. Cell Mol Biol. 5, 230-241 (1991).
[CrossRef] [PubMed]

Appl. Opt.

Appl. Phys. A

E.-L. Florin, A. Pralle, E. H. K. Stelzer, and J. K. H. Horber, "Photonic forcemicroscope calibration by thermal noise analysis," Appl. Phys. A 66, 75-78 (1998).
[CrossRef]

Biophys. J.

J. Guck, S. Schinkinger, B. Lincoln, F. Wottawah, S. Ebert, M. Romeyke, D. Lenz, H. M. Erickson, R. Ananthakrishnan, D. Mitchell, J. Kas, S. Ulvick, and C. Bilby, "Optical deformability as an inherent cell marker for testing malignant transformation and metastatic competence," Biophys. J. 88, 3689-3698 (2005).

Opt. Commun.

N. Malagnino, G. Pesce, A. Sasso, and E. Arimondo, "Measurements of trapping efficiency and stiffness inoptical tweezers," Opt. Commun. 214, 15-24 (2002).
[CrossRef]

Opt. Express

Opt. Lett.

Phys. Rev. Lett.

A. Ashkin, "Acceleration and trapping of particles by radiation pressure," Phys. Rev. Lett. 24, 156-159 (1970).
[CrossRef]

J. Guck, R. Ananthakrishnan, T. J. Moon, C. C. Cunningham, and J. Kas, "Optical deformability of soft biological dielectrics," Phys. Rev. Lett. 84, 5451-5454 (2000).
[CrossRef] [PubMed]

Other

http://www.opticsexpress.org/abstract.cfm?URI=OPEX-13-15-5798.

K.-T. Yang, M.-T. Wei, A. Karmenyan and A. Chiou " Mapping of three-dimensional optical force field on a micro-particle trapped in a fiber-optical dual-beam trap" in Optical Trapping and Optical Micromanipulation II, K. Dholakia and G. C. Spalding, eds., Proc SPIE 5930, 147-153 (2005).
[CrossRef] [PubMed]

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Figures (6)

Fig. 1.
Fig. 1.

A schematic diagram of our experimental setup

Fig. 2.
Fig. 2.

(a) A 2.58 μm silica particle trapped in a fiber-optical dual-beam trap, (b) the silica particle driven to the right by a single beam after the beam on the right hand side was turned off.

Fig. 3.
Fig. 3.

Calibration for the conversion of the QPD output voltage to the particle displacement on the optical axis via incoherent imaging on a CCD camera.

Fig. 4.
Fig. 4.

Calibration for the conversion of the QPD output voltage to the particle displacement via the power spectrum of the particle thermal fluctuation and the theoretical fitting (the red line) to a Lorentzian form.

Fig. 5.
Fig. 5.

Experimental data representing the parabolic optical force fields E(x) “□” (in black) and E(z) “□” (in blue) on a 2.58μm silica particle. The solid lines represent the theoretical fits.

Fig. 6.
Fig. 6.

CHO cell trapped and stretched in a fiber-optical dual-beam trap. Total trapping (and stretching) optical power: (a) 22mW, (b) 27mW, (c) 38mW, (d) 50mW.

Tables (1)

Tables Icon

Table. 1. A comparison of optical force constants for silica particles of different sizes and for CHO cell with and without latrunculin treatment.

Equations (3)

Equations on this page are rendered with MathJax. Learn more.

S v ( f ) = K B T / β 2 6 π 3 η r ( f c 2 + f 2 )
ρ ( z ) = C exp [ E ( z ) / K B T ]
E ( z ) = K B T ln ρ ( z ) + K B T ln C = k z Z 2 / 2

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