Using a novel dual-beam readout with the generalized phase contrast (GPC) method, a multiple-beam 3D real-time micromanipulation system requiring only one spatial light modulator (SLM) has been realized. A theoretical framework for the new GPC scheme with two parallel illumination beams is presented and corroborated with an experimental demonstration. Three-dimensional arrays of polystyrene microbeads were assembled in the newly described system. The use of air immersion objective lenses with GPC-based optical trapping allowed the simultaneous viewing of the assemblies in two orthogonal bright-field imaging perspectives.
© 2006 Optical Society of AmericaFull Article | PDF Article
René Lynge Eriksen, Vincent Ricardo Daria, and Jesper Glückstad
Opt. Express 10(14) 597-602 (2002)
Peter John Rodrigo, René Lynge Eriksen, Vincent Ricardo Daria, and Jesper Glückstad
Opt. Express 10(26) 1550-1556 (2002)
Peter John Rodrigo, Vincent Ricardo Daria, and Jesper Glückstad
Opt. Express 12(7) 1417-1425 (2004)