Abstract

In imaging systems and especially in confocal microscopy systems there is a trade-off between the lateral resolution and the obtained depth of focus. The use of complex pupils to improve the lateral resolution by engineering the point spread function is a common approach; however the lateral improvement reduces the effective depth of focus and therefore the fluorescence efficiency. In this work we analytically develop an optimized approach for obtaining a complex pupil with an extended depth of focus. The proposed solution is numerically applied and tested in designing an improved focal depth in confocal microscope configuration.

© 2010 Optical Society of America

Full Article  |  PDF Article
OSA Recommended Articles
Depth extension and sidelobe suppression in optical coherence tomography using pupil filters

Xiaojun Yu, Xinyu Liu, Jun Gu, Dongyao Cui, Junying Wu, and Linbo Liu
Opt. Express 22(22) 26956-26966 (2014)

Axial gain resolution in optical sectioning fluorescence microscopy by shaded-ring filters

M. Martínez-Corral, C. Ibáñez-López, G. Saavedra, and M. T. Caballero
Opt. Express 11(15) 1740-1745 (2003)

Considerations of aperture configuration in focal modulation microscopy from the standpoint of modulation depth

Guangjun Gao, Shau Poh Chong, Colin J. R. Sheppard, and Nanguang Chen
J. Opt. Soc. Am. A 28(4) 496-501 (2011)

References

You do not have subscription access to this journal. Citation lists with outbound citation links are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription

Cited By

You do not have subscription access to this journal. Cited by links are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription

Figures (6)

You do not have subscription access to this journal. Figure files are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription

Equations (25)

You do not have subscription access to this journal. Equations are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription

Metrics

You do not have subscription access to this journal. Article level metrics are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription