Abstract

Ratiometric fluorescent indicators are used for making quantitative measurements of a variety of physiological variables. Their utility is often limited by noise. This is the second in a series of papers describing statistical methods for denoising ratiometric data with the aim of obtaining improved quantitative estimates of variables of interest. Here, we outline a statistical optimization method that is designed for the analysis of ratiometric imaging data in which multiple measurements have been taken of systems responding to the same stimulation protocol. This method takes advantage of correlated information across multiple datasets for objectively detecting and estimating ratiometric signals. We demonstrate our method by showing results of its application on multiple, ratiometric calcium imaging experiments.

© 2008 Optical Society of America

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  1. J. Broder, A. Majumder, E. Porter, G. Srinivasmoorthy, C. Keith, J. Lauderdale, and A. Sornborger, “Estimating weak ratiometric signals in imaging data. I. Dual-channel data,” J. Opt. Soc. Am. A 24, 2921-2931 (2007).
    [CrossRef]
  2. H. Hotelling, “Relations between two sets of variates,” Biometrika 32, 38-45 (1936).
  3. J. D. Carroll, “Generalization of canonical correlation analysis to three or more sets of variables,” Proceedings of 76th Annual Convention of American Psychological Association (APA, 1968), pp. 227-228.
  4. J. Hanks, “Hanks' balanced salt solution and pH control,” Tissue Culture Association Manual 3, 3-5 (1976).
  5. K. Truong, A. Sawano, A. Mizuno, H. Hama, K. Tong, T. Mal, A. Miyawaki, and M. Ikura, “FRET-based in vivoCa2+ imaging by a new calmodulin-GFP fusion molecule,” Nat. Struct. Biol. 8, 1069-1073 (2001).
    [CrossRef]
  6. T. Anderson, An Introduction to Multivariate Statistical Analysis (Wiley, 1984).

2007 (1)

2001 (1)

K. Truong, A. Sawano, A. Mizuno, H. Hama, K. Tong, T. Mal, A. Miyawaki, and M. Ikura, “FRET-based in vivoCa2+ imaging by a new calmodulin-GFP fusion molecule,” Nat. Struct. Biol. 8, 1069-1073 (2001).
[CrossRef]

1976 (1)

J. Hanks, “Hanks' balanced salt solution and pH control,” Tissue Culture Association Manual 3, 3-5 (1976).

1936 (1)

H. Hotelling, “Relations between two sets of variates,” Biometrika 32, 38-45 (1936).

Anderson, T.

T. Anderson, An Introduction to Multivariate Statistical Analysis (Wiley, 1984).

Broder, J.

Carroll, J. D.

J. D. Carroll, “Generalization of canonical correlation analysis to three or more sets of variables,” Proceedings of 76th Annual Convention of American Psychological Association (APA, 1968), pp. 227-228.

Hama, H.

K. Truong, A. Sawano, A. Mizuno, H. Hama, K. Tong, T. Mal, A. Miyawaki, and M. Ikura, “FRET-based in vivoCa2+ imaging by a new calmodulin-GFP fusion molecule,” Nat. Struct. Biol. 8, 1069-1073 (2001).
[CrossRef]

Hanks, J.

J. Hanks, “Hanks' balanced salt solution and pH control,” Tissue Culture Association Manual 3, 3-5 (1976).

Hotelling, H.

H. Hotelling, “Relations between two sets of variates,” Biometrika 32, 38-45 (1936).

Ikura, M.

K. Truong, A. Sawano, A. Mizuno, H. Hama, K. Tong, T. Mal, A. Miyawaki, and M. Ikura, “FRET-based in vivoCa2+ imaging by a new calmodulin-GFP fusion molecule,” Nat. Struct. Biol. 8, 1069-1073 (2001).
[CrossRef]

Keith, C.

Lauderdale, J.

Majumder, A.

Mal, T.

K. Truong, A. Sawano, A. Mizuno, H. Hama, K. Tong, T. Mal, A. Miyawaki, and M. Ikura, “FRET-based in vivoCa2+ imaging by a new calmodulin-GFP fusion molecule,” Nat. Struct. Biol. 8, 1069-1073 (2001).
[CrossRef]

Miyawaki, A.

K. Truong, A. Sawano, A. Mizuno, H. Hama, K. Tong, T. Mal, A. Miyawaki, and M. Ikura, “FRET-based in vivoCa2+ imaging by a new calmodulin-GFP fusion molecule,” Nat. Struct. Biol. 8, 1069-1073 (2001).
[CrossRef]

Mizuno, A.

K. Truong, A. Sawano, A. Mizuno, H. Hama, K. Tong, T. Mal, A. Miyawaki, and M. Ikura, “FRET-based in vivoCa2+ imaging by a new calmodulin-GFP fusion molecule,” Nat. Struct. Biol. 8, 1069-1073 (2001).
[CrossRef]

Porter, E.

Sawano, A.

K. Truong, A. Sawano, A. Mizuno, H. Hama, K. Tong, T. Mal, A. Miyawaki, and M. Ikura, “FRET-based in vivoCa2+ imaging by a new calmodulin-GFP fusion molecule,” Nat. Struct. Biol. 8, 1069-1073 (2001).
[CrossRef]

Sornborger, A.

Srinivasmoorthy, G.

Tong, K.

K. Truong, A. Sawano, A. Mizuno, H. Hama, K. Tong, T. Mal, A. Miyawaki, and M. Ikura, “FRET-based in vivoCa2+ imaging by a new calmodulin-GFP fusion molecule,” Nat. Struct. Biol. 8, 1069-1073 (2001).
[CrossRef]

Truong, K.

K. Truong, A. Sawano, A. Mizuno, H. Hama, K. Tong, T. Mal, A. Miyawaki, and M. Ikura, “FRET-based in vivoCa2+ imaging by a new calmodulin-GFP fusion molecule,” Nat. Struct. Biol. 8, 1069-1073 (2001).
[CrossRef]

Biometrika (1)

H. Hotelling, “Relations between two sets of variates,” Biometrika 32, 38-45 (1936).

J. Opt. Soc. Am. A (1)

Nat. Struct. Biol. (1)

K. Truong, A. Sawano, A. Mizuno, H. Hama, K. Tong, T. Mal, A. Miyawaki, and M. Ikura, “FRET-based in vivoCa2+ imaging by a new calmodulin-GFP fusion molecule,” Nat. Struct. Biol. 8, 1069-1073 (2001).
[CrossRef]

Tissue Culture Association Manual (1)

J. Hanks, “Hanks' balanced salt solution and pH control,” Tissue Culture Association Manual 3, 3-5 (1976).

Other (2)

T. Anderson, An Introduction to Multivariate Statistical Analysis (Wiley, 1984).

J. D. Carroll, “Generalization of canonical correlation analysis to three or more sets of variables,” Proceedings of 76th Annual Convention of American Psychological Association (APA, 1968), pp. 227-228.

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