Expand this Topic clickable element to expand a topic
Skip to content
Optica Publishing Group
  • Applied Spectroscopy
  • Vol. 64,
  • Issue 11,
  • pp. 1301-1307
  • (2010)

Semi-Quantitative Analysis of Gentian Violet by Surface-Enhanced Raman Spectroscopy Using Silver Colloids

Not Accessible

Your library or personal account may give you access

Abstract

The viability of the application of surface-enhanced Raman spectroscopy (SERS) to the semi-quantitative analysis of the triphenylmethane dye gentian violet was examined by using activated borohydride-reduced silver colloids. Raman and SERS spectra of aqueous solutions of gentian violet at different pH values were acquired for the first time and equally intense SERS signals were obtained at both acidic and alkaline pH values. Two maxima intensities observed in the pH profile revealed the presence of different ionization states of the dye. The pH conditions for SERS were optimized over the pH range 1 to 12 and the biggest enhancement for SERS of this charged dye was found to be at pH 2.0; thus, this condition was used for semi-quantitative analysis. A good linear correlation was observed for the dependence of the signal intensities of the SERS bands at 1620 cm<sup>–1</sup>(<i>R</i> = 0.999) and 1370 cm<sup>–1</sup> (<i>R</i> = 0.952) on dye concentration over the range 10<sup>–6</sup> to 10<sup>–4</sup> mol/L, using laser excitation at 514.5 nm. At concentrations of dye above 10<sup>–2</sup> mol/L, the concentration dependence of the SERS signals is nonlinear. This is explained as due to the precipitation of metallic silver as well as due to saturation caused by complete coverage of the SERS substrate. A series of intensities of the band at 1620 cm<sup>–1</sup> measured from dye molecules proved that the single-molecule limit of gentian violet is attained at the concentration of 10<sup>–9</sup> mol/L.

PDF Article
More Like This
Quantitative analysis of double-stranded DNA amplified by a polymerase chain reaction employing surface-enhanced Raman spectroscopy

X. Dou, T. Takama, Y. Yamaguchi, K. Hirai, H. Yamamoto, S. Doi, and Y. Ozaki
Appl. Opt. 37(4) 759-763 (1998)

Detection of carbamazepine in saliva based on surface-enhanced Raman spectroscopy

Ning Chen, Yanbing Yuan, Ping Lu, Luyao Wang, Xuedian Zhang, Hui Chen, and Pei Ma
Biomed. Opt. Express 12(12) 7673-7688 (2021)

Cited By

You do not have subscription access to this journal. Cited by links are available to subscribers only. You may subscribe either as an Optica member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access Optica Member Subscription

Select as filters


Select Topics Cancel
© Copyright 2024 | Optica Publishing Group. All Rights Reserved