Expand this Topic clickable element to expand a topic
Skip to content
Optica Publishing Group
  • Applied Spectroscopy
  • Vol. 61,
  • Issue 5,
  • pp. 497-506
  • (2007)

Blank Augmentation Protocol for Improving the Robustness of Multivariate Calibrations

Not Accessible

Your library or personal account may give you access

Abstract

An updating procedure is described for improving the robustness of multivariate calibration models based on near-infrared spectroscopy. Employing a single blank sample containing no analyte, repeated spectra are acquired during the instrumental warm-up period. These spectra are used to capture the instrumental profile on the analysis day in a way that can be used to update a previously computed calibration model. By augmenting the original spectra of the calibration samples with a group of spectra collected from the blank sample, an updated model can be computed that incorporates any instrumental drift that has occurred. This protocol is evaluated in the context of an analysis of physiological levels of glucose in a simulated biological matrix designed to mimic blood plasma. Employing data of calibration and prediction samples acquired over approximately six months, procedures are studied for implementing the algorithm in conjunction with calibration models based on partial least squares (PLS) regression. Over the range of 1–20 mM glucose, the final algorithm achieves a standard error of prediction (SEP) of 0.79 mM when the augmented PLS model is applied to data collected 176 days after the collection of the calibration spectra. Without updating, the original PLS model produces a seriously degraded SEP of 13.4 mM.

PDF Article
More Like This
Discussion on the validity of NIR spectral data in non-invasive blood glucose sensing

Wanjie Zhang, Rong Liu, Wen Zhang, Hao Jia, and Kexin Xu
Biomed. Opt. Express 4(6) 789-802 (2013)

Determination of glucose in whole blood samples by mid-infrared spectroscopy

Yoen-Joo Kim, Sangjoon Hahn, and Gilwon Yoon
Appl. Opt. 42(4) 745-749 (2003)

Blood analysis by Raman spectroscopy

Annika M. K. Enejder, Tae-Woong Koo, Jeankun Oh, Martin Hunter, Slobodan Sasic, Michael S. Feld, and Gary L. Horowitz
Opt. Lett. 27(22) 2004-2006 (2002)

Cited By

You do not have subscription access to this journal. Cited by links are available to subscribers only. You may subscribe either as an Optica member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access Optica Member Subscription

Select as filters


Select Topics Cancel
© Copyright 2024 | Optica Publishing Group. All Rights Reserved