Abstract

A significant increase in the use of the herbicide glyphosate has generated many questions about its residual accumulation in the environment and possible damage to crops. In this study, changes in chlorophyll a (chl-a) fluorescence induced by glyphosate in three varieties of glyphosate-resistant soybean plants were determined with an in vivo analysis based on a portable laser-induced fluorescence system. Strong suppression of chl-a fluorescence was observed for all plants treated with the herbicide. Moreover, the ratio of the emission bands in the red and far-red regions (685nm/735nm) indicates that the application of glyphosate led to chlorophyll degradation. The results also indicated that the use of glyphosate, even at concentrations recommended by the manufacturer, suppressed chl-a fluorescence. In summary, this study shows that fluorescence spectroscopy can detect, in vivo, very early changes in the photosynthetic status of transgenic soybeans treated with this herbicide.

© 2013 Optical Society of America

Full Article  |  PDF Article
OSA Recommended Articles
Detection of bacterial infection of agave plants by laser-induced fluorescence

Jesús Cervantes-Martínez, Ricardo Flores-Hernández, Benjamín Rodríguez-Garay, and Fernando Santacruz-Ruvalcaba
Appl. Opt. 41(13) 2541-2545 (2002)

Feasibility of airborne detection of laser-induced fluorescence emissions from green terrestrial plants

Frank E. Hoge, R. N. Swift, and J. K. Yungel
Appl. Opt. 22(19) 2991-3000 (1983)

Analysis of laser-induced fluorescence spectra of in vitro plant tissue cultures

Ana Celia Muñoz-Muñoz, Humberto Gutiérrez-Pulido, José Manuel Rodríguez-Domínguez, Antonia Gutiérrez-Mora, Benjamín Rodríguez-Garay, and Jesús Cervantes-Martínez
Appl. Opt. 46(11) 2138-2142 (2007)

References

You do not have subscription access to this journal. Citation lists with outbound citation links are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription

Cited By

You do not have subscription access to this journal. Cited by links are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription

Figures (8)

You do not have subscription access to this journal. Figure files are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription

Metrics

You do not have subscription access to this journal. Article level metrics are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription