Expand this Topic clickable element to expand a topic
Skip to content
Optica Publishing Group

Partially coherent light-emitting diode illumination for video-rate in-line holographic microscopy

Not Accessible

Your library or personal account may give you access

Abstract

The light of a light-emitting diode or a common thermal source, such as a tungsten filament lamp, is known to be quasi-incoherent. We generated partially coherent light of these sources with a volume of coherence in the micrometer range of 5100μm3 by spatial and spectral filtering. The corresponding degree of partial coherence was adapted for microscopic interference setups, such as a digital in-line holographic microscope. The practicability of the sources was determined by the spectral emittance and the resulting signal-to-noise ratio (SNR) of the detector. The microscale coherence in correlation with the SNR and its resolution for microscopy were analyzed. We demonstrate how low-light-level, non-laser sources enable holographic imaging with a video frame rate (25frames/s), an intermediate SNR of 8 dB, and a volume of coherence of 3.4×104μm3. Holograms of objects with a lateral resolution of 1 μm were achieved using a microscope lens (50×/NA=0.7) and a CCD camera featuring a 4–12 bit dynamic range.

©2012 Optical Society of America

Full Article  |  PDF Article
More Like This
Partially coherent lensfree tomographic microscopy [Invited]

Serhan O. Isikman, Waheb Bishara, and Aydogan Ozcan
Appl. Opt. 50(34) H253-H264 (2011)

Partially coherent digital in-line holographic microscopy in characterization of a microscopic target

Tomi Pitkäaho, Mikko Niemelä, and Ville Pitkäkangas
Appl. Opt. 53(15) 3233-3240 (2014)

Supplementary Material (4)

Media 1: MOV (3985 KB)     
Media 2: MOV (3983 KB)     
Media 3: MOV (4020 KB)     
Media 4: MOV (4038 KB)     

Cited By

You do not have subscription access to this journal. Cited by links are available to subscribers only. You may subscribe either as an Optica member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access Optica Member Subscription

Figures (7)

You do not have subscription access to this journal. Figure files are available to subscribers only. You may subscribe either as an Optica member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access Optica Member Subscription

Equations (4)

You do not have subscription access to this journal. Equations are available to subscribers only. You may subscribe either as an Optica member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access Optica Member Subscription

Select as filters


Select Topics Cancel
© Copyright 2024 | Optica Publishing Group. All Rights Reserved